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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... transferred to 5 mL of LB broth (Fisher Scientific, BP1426-2), and incubated shaking overnight at 37℃ ...
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bioRxiv - Cell Biology 2020Quote: ... with single-cell passaging every 2-3 days using accutase (ThermoFisher Scientific A1110501) at split ratios between 1:5 and 1:12.
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bioRxiv - Plant Biology 2020Quote: ... from 2-3 weeks old seedlings and 500ng was treated with DNaseI (Ambion) and used for reverse transcription ...
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bioRxiv - Neuroscience 2021Quote: ... TRPA1−/− male mice were loaded with Fura-2 (3 μg/mL, Life Technologies) and pluronic acid (1:1 ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... were all purchased from Sigma and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium was purchased from Thermofisher.
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bioRxiv - Biochemistry 2020Quote: ... and cells were loaded with 3 μM Fura-2 AM dye (Molecular Probes) in sterile-filtered HEPES-Tyrode Buffer (HTB ...
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bioRxiv - Cell Biology 2021Quote: ... Primary cells were loaded with Fura-2 AM (3 μM; Invitrogen/ThermoFisher Scientific) for 45 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Primary cells were loaded with Fura-2 AM (3 μM; Invitrogen/ThermoFisher Scientific) for 45 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... p97 siRNAs (2-HSS111263 and 3-HSS111264) were from Invitrogen (Thermo Fisher Scientific). p97 rescue constructs were previously published 53 and were resistant to siRNA # 2 ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 and Caco-2 cells were cultured in minimum essential medium (GIBCO) while A549-ACE2 cells were maintained in DMEM/F-12 medium (GIBCO) ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) (M6494, Thermo Fisher Scientific) was added to each well to a final concentration of 0.67 mg/ml and incubated at 37oC ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Cells were passaged every 2-3 days using 0.05% Trypsin-EDTA (Gibco, 25300062). These cells were cultured in Knockout™ D-MEM (Gibco ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days using 0.25% trypsin-EDTA (#25200056, Gibco) to keep them below 80% confluency ...
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bioRxiv - Genetics 2023Quote: ... each coverslip was incubated in 3 μl fura-2 acetoxymethyl (AM) ester (Invitrogen) in 1X HBSS containing 5 mg/ml BSA (Sigma- Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged every 2-3 days using TryplLE™ express enzyme (GIBCO) and trypsin inhibitor (GIBCO).
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bioRxiv - Cancer Biology 2023Quote: ... Capan-2 and BxPC-3 cell lines were cultured in RPMI medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... and the pellet resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 15-20 min at 37°C to dissociate the organoids into single cells ...
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bioRxiv - Immunology 2023Quote: ... and the pellet resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 12 min at 37°C to keep the LPC spheroids as aggregates ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific). Images were captured automatically every two hours for 48 hours using the IncuCyte™ S3 Live-Cell Analysis Instrument (Essen BioScience) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following 3 days of selection with 2 µg/ml puromycin (Gibco, A11138-03), reference samples were collected (t=0 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 2-3 hours at room temperature and incubated with Hoechst 33342 (Invitrogen) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and treated with 2 µM Caspase-3/7 Green detection reagent (C10423, Invitrogen) along with the specified compounds ...
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bioRxiv - Plant Biology 2024Quote: ... 2-3 µg of total RNA were treated with DNase I (Thermo fisher), and half was reverse transcribed using the SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The final products were visualized via gel electrophoresis using 2-3% Agarose (Invitrogen) gels ...
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bioRxiv - Microbiology 2024Quote: ... which was then transferred to 2-3 mL TRIzol Reagent (Invitrogen, Waltham, MA) to form a slurry and finally thawed ...
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bioRxiv - Immunology 2024Quote: Cells were loaded with 2 µg/ml Fluo-3 AM (Thermo Fisher Scientific) and 5 µg/ml Fura Red AM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Mice were sacrificed and transcardially perfused with 2% paraformaldehyde (PFA, Merk Darmstadt, Germany) in Dulbecco’s phosphate-buffered saline (DPBS, Gibco, Paisley, UK); isolated brains were post-fixed in 2% PFA overnight and embedded in 2% low-melt agarose (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... Detached roots were then incubated in 1X PBS (Phosphate Buffered Saline) buffer with 50 μM H2DCFDA (2’, 7’-dichlorodihydrofluorescein diacetate, Invitrogen, D399) for 30 min shaking at 200 rpm/min at room temperature (Allan and Fluhr ...
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bioRxiv - Biophysics 2020Quote: ... the activated surface was incubated with fibronectin (Life Technologies, Carlsbad, USA, Cat. No. 33016015, 1.8 μg cm−2 in phosphate-buffered saline (or PBS, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Beads were washed four-times with sterile phosphate-buffered saline (PBS) to remove any residual RML6 using a DynaMag-2 (Thermo Fisher) for magnetic separation of the beads from the buffer and stored at −20 °C until further use ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50μl/well of RBD monomer at a concentration of 2 μg/ml in Dulbecco’s phosphate buffered saline (DPBS; Gibco Life Technologies). Plates were incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... samples were homogenized and ∼15g was diluted 1:2 (w/v) in cold phosphate-buffered saline (PBS; Gibco, ref.:10010-031), vortexed for 1 minute and centrifuged at 4000xg for 15 minutes at 4°C instead of 3000xg ...
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bioRxiv - Immunology 2022Quote: ... Whole blood was diluted 2-fold in calcium- and magnesium-free phosphate-buffered saline (PBS pH: 7.2; Fisher Scientific, Waltham, MA) within 30 min of collection ...
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bioRxiv - Biophysics 2023Quote: ... was washed with buffer A (0.1 M sodium phosphate, 150 mM NaCl, 0.01% Tween20, pH 7.4) using DynaMag™-2 Magnet (Thermo Fisher Scientific, 12321D). After the wash ...
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bioRxiv - Cancer Biology 2024Quote: ... MECs were placed in the fresh MEC media and incubated for 2 days before trypsinized and washed with Dulbecco’s phosphate-buffered saline (DPBS, Thermo Fisher Scientific); 5 x 105 cells in 50-100 μls DPBS (Ca2+/Mg2+ free ...
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bioRxiv - Bioengineering 2024Quote: ... Samples then underwent mechanical dissociation using “program C” on a gentle MACS dissociator (Miltenyi Biotech) and were passed through a 40 µm filter with 2 mL Dulbecco’s phosphate-buffered saline (PBS, Gibco, Cat# 14190144) added ...
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bioRxiv - Cell Biology 2020Quote: ... or DiD (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine perchlorate, aka DiIC18(5)) (Thermo Fisher #D307) at a final concentration of 4 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed 3 times in 5% Bovine Serum Albumin (BSA; ThermoFisher Scientific, 11423164) in PBS then incubated with primary antibody to H2AX (Ser139) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg mtDNA were digested with 3 ul DraI Fastdigest restriction enzyme (Thermo Scientific) at 37°C for 3h and extracted with 1 volume phenol-chloroform ...
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bioRxiv - Microbiology 2022Quote: ... FAM-5=CAT CCA ATC AAA GAC ATT GCG A 3=-TAMRA (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed 3 × 5 min and mounted on slides using Prolong Gold (Invitrogen). Deconvolution wide-field microscopy was performed using the DeltaVision Elite system equipped with an NA 1.42 60x PlanApo objective (Olympus ...
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bioRxiv - Cell Biology 2023Quote: ... The following siRNA target sequences were used: GTPBP5 siRNA: 5’-CGGUGGACACGUCAUUCUGTT-3’ (134621, Ambion); GTPBP7 siRNA ...
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bioRxiv - Cell Biology 2022Quote: ... CCDC66 was depleted using an siRNA with the sequence 5′-CAGTGTAATCAGTTCACAAtt-3′ (Thermo Scientific). Silencer Select Negative Control No ...
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bioRxiv - Cell Biology 2024Quote: ... si-PRELID1: 5’-CCCGAAUCCCUAUAGCAAA-3’) were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific, 13778075). Assays were normally carried out 48 h after transfection ...
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bioRxiv - Genomics 2024Quote: ... and a QuantStudio 3 or QuantStudio 5 Real-Time PCR instrument (Applied Biosystems™). Primers used for qPCR are listed in Suppl ...
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bioRxiv - Immunology 2024Quote: ... tonsils were rinsed 3 x 5 minutes with 1X PBS (10010023, ThermoFisher Scientific, USA) without agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... zLL 5’- and 3’- RACE PCRs were performed using the GeneRacer core kit (Invitrogen). Total RNA (2 µg ...
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bioRxiv - Cell Biology 2023Quote: ... CCDC15 was depleted using an siRNA with sequence 5′-GCAGUACCUGAGACAUAGAtt-3′ (Ambion, Cat. # s36888). For depletion of POC5 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 3-5 days as necessary using 0.5mM EDTA (Thermo Fisher). All staining and qPCR experiments included in Figure 1 were carried out in H1 and H9 stem cells ...