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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... were recloned from Addgene vectors 49435 and 49437 into pcDNA5FRT/TO and transfected into Flp-In T-REx 293 cells (ThermoFisher) to create stable cell lines with tet-inducible expression ...
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bioRxiv - Physiology 2022Quote: ... The 293-T cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; ThermoFisher Scientific, 12430112) with 10% FBS (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... American Type Culture Collection (ATCC)] was cultured in 293 SFM II medium (Thermo Fisher Scientific, MI, USA) supplemented with 2% GlutaMAX™-I (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293S GnTI- cells were grown at 37°C with 8% carbon dioxide in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... or ER GFP1-10 were generated using the Flp-In T-Rex 293 Cell Line (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... The control shCtr and shPGS1 constructs were transfected into T-REx™293 cells using lipofectamine (Invitrogen). Following transfection ...
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bioRxiv - Biochemistry 2022Quote: ... and added to 0.8 L of HEK293F cells grown in suspension in FreeStyle 293 media (Thermo Fisher). Transfected cells were incubated at 37°C and 8% CO2 for 24 hours then 1 mM Na butyrate was added and incubated at 37°C and 8% CO2 for an additional 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293F cells were grown in FreeStyle 293 Expression Medium (GIBCO™, Thermo Fisher Scientific, Mississauga, ON, Canada). All cell lines tested negative for mycoplasma using the Mycoplasma Plus™ PCR kit (#302008 ...
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bioRxiv - Microbiology 2023Quote: ... and postfusion hMPV B2 F53 were expressed by transient transfection of FreeStyle 293-F cells (Thermo Fisher) with polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies were produced by transfecting the IgG1 expression constructs using the ExpiFectamine™ 293 Transfection Kit (ThermoFisher) in Expi293F (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... were grown to a density of 1~2×106 cells/mL in FreeStyle 293 expression medium (ThermoFisher) supplemented with 0.1 mg/mL penicillin-streptomycin antibiotics ...
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bioRxiv - Biophysics 2024Quote: ... This construct was expressed in ExpiHEK-293 cell cultures using ExpiFectamine as a transfection reagent (Thermo Fisher). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... and COV-2-2196 were made by transfecting 3.60 × 108 293-Freestyle cells (ThermoFisher Scientific, Waltman, MA) in 240 mL FreeStyle 293 media (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: Plasmid was transfected to suspension Expi293F cells using Expifectamine 293 Transfection Kit (Gibco, Thermo Fisher, Cat # A14524) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2022Quote: Plasmid was transfected to suspension Expi293F cells using Expifectamine 293 Transfection Kit (Gibco, Thermo Fisher, Cat # A14524) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were produced at MAB Discovery GmbH using the FreeStyle™ 293 expression system from Thermo Fisher. Transfected HEK-293 FreeStyle™ cells were incubated for 10-11 days at 37°C ...
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bioRxiv - Biophysics 2023Quote: Flp-In™ T-REx™ 293 cells (referred to as HEK293 through the manuscript; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
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bioRxiv - Immunology 2023Quote: ... HEK293F cells in suspension used for expression were maintained in serum-free FreeStyle 293 Expression Medium (Invitrogen) at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Flp-In T-REx 293 cells were either electroporated with the Neon Transfection system (Thermo Fisher Scientific) [25] for fluorescence microscopy imaging and flow cytometry analyses or transfected using the JetPrime reagent (Polyplus ...
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bioRxiv - Plant Biology 2019Quote: ... The mixture was analyzed by immunoblotting anti-6×His (Invitrogen, #MA1-135), anti-MBP (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified using HisPur Ni-NTA Resin (Thermo Fisher). Resin-bound proteins were washed (25 mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and templated using the Ion PI Hi-Q Chef kit (Thermo Fisher). The libraries were sequenced on an Ion Proton system (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Hi-Q reagents and PI™ chip kit V3 (ThermoFisher, Waltham, USA) and the sequencing was performed for 300 flows ...
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bioRxiv - Synthetic Biology 2019Quote: ... Dual immunogold labeling was conducted with 6x-His Tag Monoclonal Antibody (Invitrogen) and the HA Tag Polyclonal Antibody as primary antibodies and an anti-mouse IgG-gold (40 nm ...
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bioRxiv - Genetics 2019Quote: To the amplification products were added formamide (Hi-Di Formamide, Applied Biosystems) and the GeneScan 500 molecular weight standard labeled with the fluorochrome LIZ ™ (orange fluorescence ...
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bioRxiv - Cancer Biology 2020Quote: ... respectively supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco). Co-cultures of JeKo-1/REC-1 with MS-5 and HS-5 at a 10:1 ratio was maintained under the same conditions as for MS-5 or HS-5 cells alone ...
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bioRxiv - Plant Biology 2021Quote: ... using the Ion PI Hi-Q OT2 200 Kit (ionTorrent, Life Technologies).
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bioRxiv - Genetics 2019Quote: pET22b-mZCWPW1-His construct was transformed into BL21 (DE3) E.Coli cells (Invitrogen). Bacterial cultures were grown to a density with O.D600∼0.7 ...
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bioRxiv - Genomics 2020Quote: All splicing reporters were cloned into pAC-5.1-V5-His (ThermoFisher Scientific) using compatible restriction sites ...
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bioRxiv - Microbiology 2020Quote: ... and 9.8 μL of highly deionized Hi-DiTM formamide (Applied Biosystems, France). Samples were analyzed by Eurofins genomics (Ebersberg ...
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bioRxiv - Biophysics 2021Quote: ... The His-MBP tag was removed by cleavage with TEV protease (Invitrogen). Further purification was achieved through capture of the cleaved protein by Strep-Tactin resin (IBA ...
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bioRxiv - Microbiology 2020Quote: ... 4 and 8 were initially cloned into pCDNA3.1/myc-His A (Invitrogen) and were later subcloned into pBJ5 vector using the NEBuilder HiFi DNA assembly kit (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... and ACE2 His constructs were produced in Expi293 cells (Thermo Fisher A14527) in Gibco Expi293 Expression Medium at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Microbiology 2021Quote: ... or His tag was purified using Protein G (cat.# 20399, Thermo Scientific) and Ni-NTA (cat.# R901100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All anCas genes were cloned into pBAD/His expression vector (ThermoFisher Scientific) and transformed in E ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal mouse anti-6x-His antibodies (Epitope-tag-clone: HIS.H8, Thermo Fisher) were employed ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS; Gibco™), 1% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The His-tagged protein was purified with Nickel-NTA resin (Thermo Scientific) in 20 mM Sodium Phosphate (pH 7.0 ...
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bioRxiv - Neuroscience 2019Quote: ... GBA was cloned into pcDNA3.1/myc-His A vector (Thermo Fisher Scientific) after digestion with EcoRI and XhoI ...
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bioRxiv - Immunology 2019Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT2 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% heat inactivated Foetal bovine serum (HI-FBS, Life Technologies), 2 mM GlutaMax (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... were coated at 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) for >4 hours at ambient temperature and blocked ≥1 hour with 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Dynabeads™ His-Tag isolation and pulldown were purchased from Thermo Fisher Scientific.
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT4 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% Heat-Inactivated Fetal Bovine Serum (HI FBS; Life Technologies), 10 mL per L of Antibiotic Antimycotic Solution (Penicillin/Streptomycin/Amphoterichin B ...
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bioRxiv - Biophysics 2022Quote: ... blots were incubated with 6x-His Tag Monoclonal Antibody (Thermo Fisher Scientific). As a control ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 2% HI-FBS and 1% penicillin-streptomycin (Gibco 15140-122). Cells were maintained in humidified incubators at 37°C and 5% CO2 ...