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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were split every 5-7 days by incubation with 0.5 μM EDTA (Thermo Fisher Scientific) for 4 min at room temperature and clumps were dissociated into small clumps by pipetting ...
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bioRxiv - Biochemistry 2023Quote: ... 7 mM NaOH) followed by an incubation of 5 hrs with Dynabeads Protein G (Invitrogen, 10004D). Beads were then washed with Wash Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mM MgCl) with Zeba™ Spin Desalting Column with a 7 kDa MWCO (Thermo Scientific). The protein was used to prepare a serial dilution in a black 384-well ...
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bioRxiv - Pathology 2022Quote: Cell viability of the MRC-5 cells cultured on LdECM and Ru-LdECM hydrogels was assessed after 1 and 7 days using Calcein AM (Thermo Scientific, Breda, the Netherlands) to stain live cells and propidium iodide (PI ...
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bioRxiv - Genomics 2020Quote: ... Cells were grown in HyClone RPMI-1640 medium (15% FBS [HyClone], 1% L-Glutamine and 1% Penicillin:streptomycin [Invitrogen]; 37°C, 5% CO2). RNA was extracted with Trizol LS (Invitrogen ...
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bioRxiv - Immunology 2022Quote: A culture flask containing 6.0 × 106 Vero cells was infected for one hour at 37°C with 0.01 MOI ZIKV in 5 mL of MEM medium with 2% FBS (Serum Fetal bovine; Thermo Fisher Scientific, cat: 12657029). After one hour of infection ...
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bioRxiv - Molecular Biology 2019Quote: ... 10% glycerol and 5 mM β-mercaptoethanol) and then TEV cleaved for 2 hours at 16°C using AcTEV™ protease (Invitrogen, Germany). TEV elute was digested with RNace-It™ Ribonuclease Cocktail (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... glucose-free RPMI 1640 and incubated at 37 °C and 5% CO2 for 2 hours in glucose-free RPMI (Thermo Fisher Scientific, 11879020) supplemented with 10 mM 13C6-glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 (colorectal adenocarcinoma, ATCC® HTB-37) were grown at 37 °C under 5% CO2 in DMEM low glucose pyruvate (Gibco, 31885-023) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were dehydrated by sequential submersion in 100% ethanol (2× 5 min) and SafeClear II (2× 5 min, Fisher Scientific) before being mounted in Cytoseal 60 (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... differentiated Th2 cells were restimulated with anti-mouse CD3 (4 µg/mL) for 3 hours followed by 2 hours of incubation with monensin (Thermo Fisher Scientific, MA, USA) at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 52h after plating 5 µM 5-ethynyl-2′-deoxyuridine (EdU, Life Technologies) was added for 20 h ...
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bioRxiv - Microbiology 2024Quote: ... for 1h at 37°C and then with 3 µM DAPI (Invitrogen) for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then passaged 1:3 as clumps using 15 min 37°C incubation with 1mg/ml collagenase IV (Invitrogen) onto plates coated with 1:50 GFR-Matrigel (Corning ...
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bioRxiv - Immunology 2022Quote: ... Heat inactivated serum samples (56°C for 1 hour) were serially diluted (3-fold) in minimum essential media (MEM; Gibco) supplemented with 2 mM L-glutamine (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA-treated cells were washed twice with 37°C M1 and replaced with M1 containing 1 μM TO-PRO-3 iodide (ThermoFisher) or washed once with 37°C M2 containing 5 mM ethylene glycol-bis(2-aminoethylether)-N ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted in 100 µL of nuclease free water and precipitated at -80 °C for ≥ 3 hours with 1 µL of 15 mg/mL GlycoBlue coprecipitant (ThermoFisher), 10 µL of 3 M sodium acetate pH 5.5 ...
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bioRxiv - Microbiology 2024Quote: OG1RF cultures were grown overnight in 3 mL of CBM at 37 °C and diluted 1:50 into an optically clear 96-well plate (Nunc MicroWell tissue culture- treated optical bottom plates) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was incubated overnight at 4 °C with beads (Invitrogen) previously conjugated with 1 µg of mouse monoclonal anti-human TRAP1 (sc-73604 ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C and the supernatant added to Ni-NTA Agarose (Invitrogen) equilibrated in His-tag buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... at +4 °C using a SnakeSkin dialysis membrane (Thermo Fisher Scientific) with a 3.5 kDa molecular-weight cutoff ...
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bioRxiv - Physiology 2024Quote: ... were incubated overnight at 4°C and the secondary antibodies (Invitrogen) were incubated for 1 h at room temperature.
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bioRxiv - Cell Biology 2024Quote: ... for overnight at 4°C and Alexa-labeled secondary antibodies (Invitrogen, A11006 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were immobilized overnight at 4°C on Maxisorb™ (Nunc) 96 wells microplates at 1µg/well in 100 µl NaHCO3 0.1 M pH 8.9 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated in primary antibody overnight at 4°C (rabbit anti-GFP 1:1000; Invitrogen A-6455 RRID:AB_221570), and in secondary antibody at 1:500 for 3 hours at room temperature goat (anti-rabbit Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2022Quote: ... at 4°C overnight followed by donkey anti-rabbit Alexa 488 secondary antibody (1:1000; A32766, Thermo Fisher Scientific), donkey anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... Oocytes were injected with 0.1 to 10 ng cRNA of mASIC1a (volumes between 9 and 50 nL) and incubated for 1–4 days at 18 °C in Leibovitz’s L-15 medium (Gibco), supplemented with 3 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were first incubated overnight at 4°C in rabbit anti-CALR (1:500, ThermoFisher, Waltham, MA, cat# PA3900) then goat anti-rabbit Alexa Fluor 488 (1:2000 ...
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bioRxiv - Neuroscience 2019Quote: ... at 4 °C followed by incubation the next day with goat anti-chicken antibody (647nm, A21449, ThermoFisher, 1:600) and goat anti-rabbit antibody (568 nm ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with primary antibodies (in 0.01 M PBS with 1% BSA and 0.3% Triton X-100) overnight at 4 °C and subsequently with corresponding secondary antibodies (1:1,000, Alexa-Fluor-conjugated, Invitrogen). DAPI was used to label the nuclei and sections were mounted with 75% glycerol ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting supernatant was centrifuged at 20,000 ×g for 1 hour at 4°C (Sorvall WX 100+ Ultracentrifuge, ThermoFisher) to pellet large EVs and the remaining supernatant was again centrifuged at 100,000 ×g for 1 hour at 4°C to pellet crude small EVs ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated overnight at 4 °C with primary antibody against ZO-1 (7.5 μg/ml, Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Molecular Biology 2021Quote: ... Tubes were incubated for 1 hour at 4°C and then protein A/G agarose magnetic beads (Thermo Scientific) were added for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The organoids were incubated overnight at 4◦C with a primary antibody against KI67 (Invitrogen #MA5-14520; 1:200) or MUC2 (Santa Cruz #sc-15334 ...
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bioRxiv - Cell Biology 2020Quote: ... islets were similarly incubated for 1 hr in imaging buffer at 37 °C with the addition of 2.5 μM Fluo-4 (Invitrogen). Islets incubated with Fluo-4 were washed three times with imaging buffer prior to imaging ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were precleared by incubation for 1 hour at 4°C on a rotator with 10μl Protein G magnetic beads (ThermoFisher). The indicated antibody was incubated in 600μl IP buffer containing 1μl 10mg/ml acetylated BSA and 5μl Protein G magnetic beads for 4 hours with rotation at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Crosslinking was reversed by incubating the beads at 65°C for 4 hr in the presence of 0.3% SDS and 1 mg/ml of Proteinase K (Ambion). DNA was purified using Zymo ChIP DNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then incubated overnight at 4°C with a 1:500 dilution of rabbit anti-HA antibody (Invitrogen) in PBS 1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and with anti-rabbit IgG Alex Fluor 633 (ThermoFisher, Cat# A-21070, dilution 1:2000) for one hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... lysate from mock transfected cells was incubated at 4°C for 1 hour with Sepharose-G beads (Fisher Scientific), previously washed with 5% BSA in TBS-Triton buffer and preincubated with 2.5 μg anti-GFP antibody ...
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bioRxiv - Neuroscience 2021Quote: ... at 14000 × g at 4 °C for 1 min and loaded into an HPLC system (Thermo Scientific Ultimate 3000). The mobile phase was 12.5% acetonitrile buffer (pH 3.0 ...
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bioRxiv - Neuroscience 2019Quote: Tissue from anonymized donors was received at 4°C in 1 ml “fibroblast medium” containing MEM alpha (Gibco, #12571), 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with primary antibodies (anti-ACE2 (1:1000; MA5-32307 - Thermo Fisher), anti-Sigma-1 R (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Precipitated virus particles were pelleted by centrifugation at 1,600 x g for 1 hour at 4 °C and resuspended with DMEM (GIBCO). Concentrated virus particles were frozen and stored at − 80 °C until use.
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bioRxiv - Neuroscience 2021Quote: ... at 4°C for 1hr and 45 minutes in 1X NuPAGE® Transfer Buffer (Life Technologies, cat# NP0006-1). Blocking and antibody incubation was performed in 2% BSA (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with rabbit anti-ApoE antibody (1:200, #701241, Invitrogen, Carlsbad, CA, USA) and either rat anti-CD31 antibody (1:200 ...
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bioRxiv - Immunology 2020Quote: ... Baker) for 45 min at 4 °C and then permeabilized with either 1 × Permeabilization Buffer (transcriptional factor staining, ThermoFisher) or 1 × Perm/Wash Buffer (cytokine staining ...
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bioRxiv - Immunology 2021Quote: ... Blots were incubated overnight at 4°C with agitation in blocking buffer and anti-HisG-HRP (1:5000, Invitrogen), or anti-V5-HRP (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... CCR10 (248918) from R&D, CD11a (M17/4) from eBioscience, FcεRI (MAR-1), c-Kit (CD117, 2B8) from Invitrogen and Mouse BD Fc Block™ ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and with anti-rat IgG Alex Fluor 488 (ThermoFisher, Cat# A-11006, dilution 1:1000), anti-guinea pig IgG Alex Fluor 568 (ThermoFisher ...