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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... cells were cultured and transiently co-transfected with wild-type or mutant Nav1.2 channel variant (5 μg) and enhanced green fluorescent protein (1 μg) using Lipofectamine 3000 kit (Thermo Fisher Scientific), as previously described (26) ...
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bioRxiv - Genomics 2023Quote: ... for 5 min at room temperature and washed them three times with 1× PBS (diluted from 10× PBS Liquid Concentrate, Gibco, USA). We then lysed parasites with 0.1% Sarkosyl Solution (Bioworld ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Developmental Biology 2024Quote: ... manually minced and enzymatically digested in an orbital shaker for 2 hours at 37°C in digestion mix (9.2 ml DMEM/F12, 5% FCS, 1% Penicillin/Streptomycin, 25 mM HEPES (Gibco, cat. #15630056) and 300 U/ml Collagenase IV (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Glycine was then aspirated and cells permeabilised and blocked for 5 minutes with PBS containing 1% bovine serum albumin (BSA) (BP1600-100, Fisher Scientific) and 0.1% saponin (84510-100G ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3 μg of RNA was used for cDNA synthesis and 1 μl of 1:5 diluted cDNA was used in the RT-qPCR reaction triplicate (Luminaris Color HiGreen qPCR master mix, Thermo Scientific). Efficiencies of primers was calculated from standard curves to ensure similar amplification efficiencies between quantified genes ...
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bioRxiv - Immunology 2024Quote: ... major Friedlin (MHOM/SU/73/5-ASKH) strain (1×105 logarithmic promastigotes per mL) were cultured at 26 °C in M199 medium (Gibco, USA) (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed using RIPA lysis buffer (0.1% SDS, BP8200100, Fisher Scientific, 1% IPEGAL CA630, 8896, EMD-Millipore; 0.5% sodium deoxycholate, D6750, Sigma, 50mM Tris, BP152-5, Fisher Scientific; 150mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were washed with PBS three times for 5 min and incubated with secondary antibodies (goat anti-rabbit-AlexaFluor488: 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... 2 µl drops of yeast suspension were plated in five 1:5 serial dilutions on 100 mm square plates (Thermo Fisher) with selective SD medium and increasing concentrations (0.07–7 µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and NOD2−/− primary microglia treated with M18-MDP (1 μg/ml) or α-Syn PFF (5 μg/ml) were lysed in ubiquitination lysis buffer (#PI89900, ThermoFisher), supplemented with protease inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... brains were then washed four times in 0.05% PBS-T and then incubated with 5% NGS and secondary antibody (1:400 Alexa 594 goat anti-mouse IgG, Invitrogen A11032) in 0.5% PBS-T for 2 days at 4 °C with nutation ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed and incubated with the secondary antibody dilution (Horse anti-mouse, HRP-ligated, PI 2000, 1:5000 in 5%dry milk, Thermo Fisher) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated with blocking solution (1× PBS with 5% Normal Goat Serum [50-062Z; ThermoFisher Scientific], 0.1% Triton X-100) for 15 min and with primary antibodies solutions for 2 to 3 hours at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen, G401002). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Neuroscience 2024Quote: ... Fluorescein or Texas Red labelled dextran (0.1mL of 1-5% of 70kDa dextran solution in saline; Sigma-Aldrich #46945 or Thermofisher D1830) was injected intravenously immediately before the start of imaging to visualize blood flow ...
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bioRxiv - Microbiology 2024Quote: ... and 5% O2 in complete RPMI culture medium (cRPMI) composed of iRPMI 1640 with 1% AlbuMax I (Life Technologies, Carlsbad, CA), 0.21% sodium bicarbonate (KD Medical ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was then diluted (1:5) with RNAase/DNAase-free water and qRT-PCR performed using SYBR-Green Master Mix (ThermoFisher, #4309155) and primers listed in Supplementary Data ...
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bioRxiv - Microbiology 2024Quote: ... and mounted on glass slides of 1.5 mm thickness (Fisherbrand) and covered with #1 thickness glass coverslips (Fisherbrand) using the ProLong Gold Antifade mounting medium (ThermoFisher Scientific). Images were obtained using a Nikon C2 confocal system and a Plan Apo 60X oil/1.40NA objective ...
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bioRxiv - Immunology 2024Quote: ... 1 × TE buffer (5 mM Tris base, pH 8.0 and 1 mM EDTA acid) containing 15% w/v PEG-8000 (Fisher Scientific, BP2331) and 510 mM NaCl was added to the SQB sample at 1:1 volume and mixed gently via pipetting ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed with TBS-T buffer and blocked with either 5% milk diluted in TBS-T or 1% casein in TBS (ThermoFisher: 37532) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted fragment solution was then directly injected at a rate of 5 μL min-1 into the Q-exactive Plus Mass Spectrometer (Thermo Scientific) and measured in negative mode ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time PCR was performed in triplicates using 1 µl cDNA and 9 µl master mix consisting of 5 µl SYBR Green PCR Mix (4309155, Applied Biosystems), 3 µl H2O and 1 µl of forward and reverse primers (0.5 µM final concentration) ...
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bioRxiv - Cancer Biology 2024Quote: ... while a global protein aliquot was resuspended in 50 μl of 0.1% FA and 5 µl was loaded twice onto a Dionex U3000 RSLC in front of an Orbitrap Eclipse (Thermo Fisher) equipped with an EasySpray ion source ...
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bioRxiv - Cell Biology 2024Quote: ... cells were cultured for 72 h in RPMI 1640 medium supplemented with 1% pen-strep and 5% exosome-depleted FBS (GibcoTM, ThermoFisher Scientific). sEV derived from A549 cells were isolated from the conditioned medium by differential ultracentrifugation ...
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bioRxiv - Bioengineering 2023Quote: ... A total of 20 µL mixture was loaded into 384-well plate and centrifuged at 1000 rpm for 1 min before running the Melting Curve program on QuantStudio 5 Real-Time PCR System (Applied Biosystems). circRNA underwent a denaturing process at 95°C for 5 min with a ramp rate of 0.05°C/s starting from 25°C ...
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bioRxiv - Microbiology 2024Quote: ... The parasites were washed three times for 5 min in 1 x PBS before the second antibody (Alexa Fluor 647-conjugated goat anti-Rabbit antibody (Life Technologies)) ...
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bioRxiv - Genetics 2019Quote: ... Slides were washed three times in PBS (5 min each) and incubated with secondary antisera (AlexaFluor594-conjugated goat anti-mouse antibody;Life Technologies A11005; 1:1000 in 1% skimmed milk, PBS), 150 μl per slide ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Biochemistry 2020Quote: ... soluble form of HO2 spanning residues 1-248 (the core of the protein) was expressed from the pET28a vector in BL21(DE) cells (Life Technologies) and purified by Ni-NTA– agarose affinity chromatography (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... De novo synthesis of Sgo1 protein was quenched by the addition of 2% glucose and 1 mg/mL cycloheximide (Acros Organics).
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bioRxiv - Microbiology 2022Quote: BHK-21 [C-13] and HepG2 cell lines were purchased from Banco de Células do Rio de Janeiro (BCRJ) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, GIBCO) containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Immunology 2021Quote: Thymi from 4-week-old wild type mice were harvested and 5 million thymocytes per condition were cultured in IMDM medium (Gibco) with 2% FBS (HyClone Thermo Fischer Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Alkaline phosphatase staining was performed using the one-step nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP) solution (Thermofisher).
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bioRxiv - Molecular Biology 2020Quote: ... produced from DKO piglet were prepared and stained at 4°C for 45 minutes with Alexa 488-conjugated Griffonia simplicfolia isolectin IB4 (GS-IB4) (5 μg/mL, Invitrogen). The stained cells were washed twice and then analyzed by flow cytometer (BD Accuri™ C6 Plus) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were boiled at 95°C for 5 min before running on a NuPAGE 4-12% Bis-Tris protein gradient mini gel (Invitrogen) at 200 V for 5 min in MOPS running buffer (Invitrogen ...
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bioRxiv - Genomics 2020Quote: Neuronal suspensions were labelled at 37°C with a combination of the Fluo-4 calcium indicator (5 μg/ml; Invitrogen) and either Alexa-Fluor 594-coupled Wheat Germ Agglutinin (WGA ...
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bioRxiv - Cell Biology 2019Quote: ... 10-15% of RIPA-soluble fraction and 2.5-5% of insoluble fraction were loaded on 4-12% Bis-Tris gels and run using MOPS buffer (Life Technologies) and visualized by western blotting on PVDF ...
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bioRxiv - Genetics 2020Quote: ... For morphological analyses 105 cells were resuspended in 200 ml PBS and spun (5 min, 400 rpm) in a Cytospin 4 (ThermoFisher), before staining with modified Wright’s Stain on a Hematek (Bayer Health Care) ...
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bioRxiv - Genetics 2021Quote: ... 100 µL of the cell suspension were then placed in a cytofunnel and spun at 1200 rpm for 5 min with high acceleration using a cytocentrifuge (Shandon Cytospin 4; ThermoFisher). For FISH ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 4-12% Novex WedgeWell Tris-Glycine gels (ThermoFisher).
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bioRxiv - Bioengineering 2021Quote: ... 10 µg of each sample was boiled at 95°C for 5 min and loaded onto the 4-12% Bis-Tris Gel (NP0336, ThermoFisher). Gels were run for 1 hour and 15 min at 120 V in with NuPage MES Buffer (NP0002 ...
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bioRxiv - Biophysics 2021Quote: Cells were magnetically labelled thanks to an incubation of 2h with a solution of iron oxide nanoparticles at [Fe] = 4 mM and supplemented with 5 mM citrate in RPMI medium (Gibco). The viability and the proliferation of cells after magnetic labelling were assessed by Alamar Blue showing no impact of the magnetic labelling right after the labelling or after one day (Supplementary Figure 1).
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bioRxiv - Biochemistry 2021Quote: ... and samples were incubated at 70°C for 5 minutes and loaded onto Bis-Tris 4-12 % gradient gels (ThermoFisher). Bands were stained by CBB and relative band intensity was quantified by scanning and semi-automated analysis in ImageQuant (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... and anti-CD127-PE-Cy5 (clone eBioRDR5; 5 μL; cat. # 15-1278-42) all from Thermo Fisher (Supplementary Fig. 4). mAbs for chemokine receptors (i.e ...