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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: BP were dissected in cold L-15 media and incubated for 1 hour at room temperature with 5 µM pHrodo Red Intracellular pH Indicator (Invitrogen P35372) and 1 nM SiR-actin (SpiroChrome SC001 ...
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 in 5% BSA in TBST was used for viral loading control and α-GAPDH primary antibody (Invitrogen #AM4300) at 1:1000 in 5% BSA in TBST for cellular loading control ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatographic separation was carried out using a Hypercarb porous graphitic carbon (PGC) column (5 µm, 150 mm x 1 mm, Thermo Scientific). A 40-minute binary gradient using 3% ACN in water containing 0.1% formic acid (Solvent A ...
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bioRxiv - Cell Biology 2023Quote: ... then with the following antibodies in 5% BSA for 1 h: donkey anti-rabbit IgG Alexa Fluor 488 (A21206, Thermo Fisher) and α-Bungarotoxin Alexa Fluor 594 (B13423 ...
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bioRxiv - Cell Biology 2023Quote: ... the beads were collected by centrifugation and washed with inhibitor-containing lysis buffer 5 times before being mixed with 20-40 μl 1× Laemmli sample buffer (diluted from Invitrogen, NP0008) supplemented with β-mercaptoethanol ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then blocked with a 5% (v/v) FBS solution and incubated for 90 min in a 1:200 phalloidin–Alexa Fluor 594 (Life Technologies) diluted in a 1% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... cells were cultured and transiently co-transfected with wild-type or mutant Nav1.2 channel variant (5 μg) and enhanced green fluorescent protein (1 μg) using Lipofectamine 3000 kit (Thermo Fisher Scientific), as previously described (26) ...
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bioRxiv - Genomics 2023Quote: ... for 5 min at room temperature and washed them three times with 1× PBS (diluted from 10× PBS Liquid Concentrate, Gibco, USA). We then lysed parasites with 0.1% Sarkosyl Solution (Bioworld ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Developmental Biology 2024Quote: ... Enzymatic digestion was stopped by adding 500 μl of HBSS+ (HBSS, 5% Fetal Bovine Serum, 1% HEPES (Thermo Fisher, 15630-080) 1M ...
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bioRxiv - Microbiology 2024Quote: ... The dry pooled sample was resuspended in 1 ml of 5% formic acid and was desalted using the EasyPep Maxi Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... mouse calvariae were dissected from 1–3 days old neonatal mice and digested sequentially 5 times for 25 minutes in α-MEM (Gibco) containing 0.1% collagenase (Roche ...
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bioRxiv - Biophysics 2024Quote: ... wild-type α-synuclein was mixed with an A90C variant labelled with Alexa 647 at a 100:1 molar ratio in 50 mM Tris-HCL and 5% polyethylene glycol 10,000 (PEG) (Thermo Fisher Scientific). The final mixture was pipetted onto a 35-mm glass-bottom dish (P35G-1.5-20-C ...
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bioRxiv - Neuroscience 2024Quote: Brain sections were blocked and permeabilized for 1 hour in 1xPBS containing 5% normal goat serum (ref # 16210064, Thermo Fisher Scientific) and 0.5% Triton X-100 (ref # BP151 ...
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bioRxiv - Bioengineering 2024Quote: ... in 50 µL of PBS + 5% FBS for 30 minutes followed by a 1:1000 dilution of Alexa Fluor 488-conjugated secondary antibody (Invitrogen; A10631) in 50 µL of PBS + 5% FBS for another 30 minutes.
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bioRxiv - Microbiology 2024Quote: ... The grids were immediately blotted for 1-5 s and plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) device operating at 4 °C and 100% humidity ...
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bioRxiv - Biophysics 2024Quote: ... The resulting peptides are trapped and desalted on a C18 trapping column (Acclaim 5 µm, PepMap 300, 1 mm x 15 mm, Fisher Scientific). Once desalting is complete the trap column is placed in line with a separation column (Hypersil GOLD 1.9 µm particle size ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 monocytes were cultured at 37°C with 5% CO2 to confluence in RPMI media (MEDIUM 1640 CE, Gibco, 21875034) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and the flowthrough was ethanol precipitated in the presence of 5 mM MgCl2 and 1 µL GlycoBlue (Invitrogen, Cat. No. AM9516). The RNA pellet was resuspended in 10 µL of RNase-free water and immediately processed for library preparation.
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 3-5 minutes at 37°C with a 1:10 dilution of trypsin 2.5% (Thermo Fisher #15090-046) in PBS (HSCs ...
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bioRxiv - Genetics 2024Quote: ... Samples were resuspended in 50 ul lysis buffer (50 mM Na-HEPES pH 7.5, 200 mM NaOAc, 1 mM EDTA, 0.25% NP-40, 3mM DTT, and 1X Halt Protease Inhibitor Cocktail [Thermo Fisher Scientific]). Approximately 100 ul of 0.5 mm glass beads were added to each sample ...
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Leveraging single cell multiomic analyses to identify factors that drive human chondrocyte cell fatebioRxiv - Developmental Biology 2024Quote: ... and cultured in suspension in the presence of BMP4 (1 ng/mL) and Y-27632 (5 μM) in Stem-Pro-34 media (Life Technologies) supplemented with L-glutamine ...
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bioRxiv - Synthetic Biology 2024Quote: ... and afterwards stained for 1 h in 50 ml of 0.5X TBE buffer supplemented with 5 µl of SYBR Gold dye (Invitrogen, Waltham, MA) while gently shaken ...
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bioRxiv - Genetics 2019Quote: ... Slides were washed three times in PBS (5 min each) and incubated with secondary antisera (AlexaFluor594-conjugated goat anti-mouse antibody;Life Technologies A11005; 1:1000 in 1% skimmed milk, PBS), 150 μl per slide ...
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bioRxiv - Biochemistry 2020Quote: ... soluble form of HO2 spanning residues 1-248 (the core of the protein) was expressed from the pET28a vector in BL21(DE) cells (Life Technologies) and purified by Ni-NTA– agarose affinity chromatography (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... De novo synthesis of Sgo1 protein was quenched by the addition of 2% glucose and 1 mg/mL cycloheximide (Acros Organics).
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Microbiology 2022Quote: BHK-21 [C-13] and HepG2 cell lines were purchased from Banco de Células do Rio de Janeiro (BCRJ) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, GIBCO) containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Immunology 2021Quote: Thymi from 4-week-old wild type mice were harvested and 5 million thymocytes per condition were cultured in IMDM medium (Gibco) with 2% FBS (HyClone Thermo Fischer Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Alkaline phosphatase staining was performed using the one-step nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP) solution (Thermofisher).
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bioRxiv - Molecular Biology 2020Quote: ... produced from DKO piglet were prepared and stained at 4°C for 45 minutes with Alexa 488-conjugated Griffonia simplicfolia isolectin IB4 (GS-IB4) (5 μg/mL, Invitrogen). The stained cells were washed twice and then analyzed by flow cytometer (BD Accuri™ C6 Plus) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were boiled at 95°C for 5 min before running on a NuPAGE 4-12% Bis-Tris protein gradient mini gel (Invitrogen) at 200 V for 5 min in MOPS running buffer (Invitrogen ...
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bioRxiv - Genomics 2020Quote: Neuronal suspensions were labelled at 37°C with a combination of the Fluo-4 calcium indicator (5 μg/ml; Invitrogen) and either Alexa-Fluor 594-coupled Wheat Germ Agglutinin (WGA ...
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bioRxiv - Cell Biology 2019Quote: ... 10-15% of RIPA-soluble fraction and 2.5-5% of insoluble fraction were loaded on 4-12% Bis-Tris gels and run using MOPS buffer (Life Technologies) and visualized by western blotting on PVDF ...
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bioRxiv - Genetics 2020Quote: ... For morphological analyses 105 cells were resuspended in 200 ml PBS and spun (5 min, 400 rpm) in a Cytospin 4 (ThermoFisher), before staining with modified Wright’s Stain on a Hematek (Bayer Health Care) ...
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bioRxiv - Genetics 2021Quote: ... 100 µL of the cell suspension were then placed in a cytofunnel and spun at 1200 rpm for 5 min with high acceleration using a cytocentrifuge (Shandon Cytospin 4; ThermoFisher). For FISH ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 4-12% Novex WedgeWell Tris-Glycine gels (ThermoFisher).
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bioRxiv - Bioengineering 2021Quote: ... 10 µg of each sample was boiled at 95°C for 5 min and loaded onto the 4-12% Bis-Tris Gel (NP0336, ThermoFisher). Gels were run for 1 hour and 15 min at 120 V in with NuPage MES Buffer (NP0002 ...
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bioRxiv - Biophysics 2021Quote: Cells were magnetically labelled thanks to an incubation of 2h with a solution of iron oxide nanoparticles at [Fe] = 4 mM and supplemented with 5 mM citrate in RPMI medium (Gibco). The viability and the proliferation of cells after magnetic labelling were assessed by Alamar Blue showing no impact of the magnetic labelling right after the labelling or after one day (Supplementary Figure 1).
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bioRxiv - Biochemistry 2021Quote: ... and samples were incubated at 70°C for 5 minutes and loaded onto Bis-Tris 4-12 % gradient gels (ThermoFisher). Bands were stained by CBB and relative band intensity was quantified by scanning and semi-automated analysis in ImageQuant (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... and anti-CD127-PE-Cy5 (clone eBioRDR5; 5 μL; cat. # 15-1278-42) all from Thermo Fisher (Supplementary Fig. 4). mAbs for chemokine receptors (i.e ...
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bioRxiv - Cell Biology 2021Quote: ... 5–12 µl of protein lysate was loaded into pre-cast protein gels (4–12 % Bolt Bis Tris Plus, ThermoFisher) together with 10 µl of the molecular marker (PageRuler prestained ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Systems Biology 2021Quote: ... and samples were centrifuged at 16,000g for 5 minutes at −4°C in a Sorvall Legend Micro 17R microcentrifuge (Thermo Scientific). The supernatant (extract ...