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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco, 31985062). Approximately 24 h later ...
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bioRxiv - Neuroscience 2022Quote: ... We next rinsed 3 x 5 min with PBS and incubated with appropriate secondary antibodies conjugated to Alexa488 or Alexa647 fluorophores (1:250, Molecular Probes) at RT for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked for 30 minutes using 5% skimmed milk in PBST and then probed with rat anti-GFAP primary antibody (1:2000, cat # 13-0300, Thermo Scientific) overnight at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Neuroscience 2022Quote: ... SH-SY5Y cells were replated into primary astrocytes in 6-well plates in density 1.103cells/cm2and co-cultivated for further 5 days in DMEM medium (PAA Laboratories GmbH, Germany) containing 1% FCS (Invitrogen, Carlsbad, CA), 2 mM L-glutamine (PAA Laboratories GmbH ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were run on 1% Agarose-LE gels at low voltage for 4 hours along with 1ng each of positive control positive sense segment 1 and segment 5 transcripts and RiboRuler High Range RNA ladder (Thermo Fisher). Nucleic acids were blotted on to Nytran SuPerCharge nylon membranes (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed with PBS (3x 5 min) and incubated in donkey-anti-guinea pig 647 (1:1000; ThermoFisher, Cat# A21450) in antibody diluent for 2h at RT ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4.75 μL of nuclease-free water and 400 nM of each primer or by adding 1 µl of yeast lysate to 5 µl of DreamTaq Green PCR Master Mix (2X) (ThermoFisher Scientific) plus 4 µL of nuclease-free water with 200 nM of each primer ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS and then incubated in PBS containing 5 % normal goat serum with fluorescent conjugated secondary antibodies (Alexa Fluor 555-conjugated goat anti-mouse IgG, 1:500, Thermo Fisher, A21422 ...
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bioRxiv - Biophysics 2022Quote: ... Frogs are euthanized by immersion for 10 min in 1 L water + 5 g Tricaine-S (Pentair via Fisher Scientific #NC0342409) + 10 g sodium bicarbonate (Fisher Scientific # MK-7396-500) ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed three times for 5 min in TBS-T at room temperature and incubated in 1:10000 secondary anti-Rabbit-HRP (Invitrogen, #31460) in TBS-T/Milk 5% for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained in phosphate buffered saline containing 1 μg/mL PI and 5 μg/mL Hoechst 33342 (Thermo Fisher Scientific) and the average number of PI- live cells per condition was determined using a CellInsight CX5 High Content Screening Platform ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% Penicillin Streptomycin at 37 °C and 5% CO2 and split when confluent using Trypsin-EDTA (0.05%) (Life Technologies, 25300054). When indicated ...
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bioRxiv - Biochemistry 2023Quote: ... washed twice in DMEM with 10% CCS and suspended in Williams E medium supplemented with 5 % CCS and 1 mM glutamine (Invitrogen, CA). To separate live from dead cells ...
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bioRxiv - Immunology 2023Quote: ... in PBS for 1 h at 25°C followed by permeabilization in 5% BSA in the presence of 0.05% Tween-20 (Thermo Fisher Scientific) for 15 minutes at 25°C ...
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bioRxiv - Biophysics 2023Quote: ... 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio) were mixed with 15 ml FreeStyle 293 media (Thermo Fisher) and 1.2 ml of PEI (Polysciences ...
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bioRxiv - Developmental Biology 2023Quote: BP were dissected in cold L-15 media and incubated for 1 hour at room temperature with 5 µM pHrodo Red Intracellular pH Indicator (Invitrogen P35372) and 1 nM SiR-actin (SpiroChrome SC001 ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged every 5-6 days as small clumps by dissociation with a buffer containing 1 mg per ml Collagenase IV (ThermoFisher Scientific), 0.025% Trypsin (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were washed 3 times for 5 min with PBTx before incubation at room temperature in 1 μg/mL DAPI (Invitrogen, ThermoFisher Scientific) solution in PBS for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by a 5 min incubation with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) and revealed using a Chemidoc MP Immaging System (BIO-RAD) ...
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bioRxiv - Molecular Biology 2023Quote: ... Serial 10-fold dilutions of gene fragments were performed in sheared salmon sperm DNA 5 µg mL-1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Immunology 2023Quote: ... 2.5 µl of a 200 nM stock pool of each primer pair (Supplementary File 1) was added to 5 µl of TaqMan PreAmp Master Mix (Applied Biosystems) and 2.5 µl of 1:5 dilution of cDNA ...
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bioRxiv - Microbiology 2023Quote: ... The azide group of ADA-DA was labelled (post-fixation) with 5 µg ml−1 Alexa Fluor 488 Alkyne using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen) according to the manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... A 1:5 dilution of cDNA and custom primers were input into qPCR reactions with PowerUP SYBR Green Master Mix (Applied Biosystems).qRT-PCR was performed using a QuantStudio 3 Real Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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bioRxiv - Microbiology 2023Quote: ... 1X MOPS (20 mM MOPS, 5 mM NaOAc, 1 mM EDTA, pH 7.0) and 1X RNA loading dye (Thermo Fisher Scientific) for 10 min at 70°C and incubated on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... The tops of the micropillars were coated with a mixture of unlabeled and Alexa Fluor 647-labeled fibronectin (1:5, Life Technologies) using micro-contact printing ...
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bioRxiv - Biochemistry 2022Quote: ... Labeling was performed by the addition of dyes at a molar ratio of 5:1 dye:nanobody using Alexa 488 NHS (Thermo Fisher Scientific) for the GFP nanobody and Alexa 568 NHS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse transcribed and amplified in 5 reactions with 1 µg RNA template using SuperScript IV One-Step RT-PCR System with ezDNase (Invitrogen, 12595025) and the primers ...
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bioRxiv - Cancer Biology 2023Quote: For sEVs isolation cells were cultured for 72 h in RPMI 1640 medium supplemented with 1% pen-strep and 5% exosome-depleted FBS (GibcoTM, ThermoFisher Scientific). sEV were isolated from the conditioned medium (CM ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 mg/ml fibrinogen and 0.2% LAP for photoinitiation and cultivated in vitro for up to 5 days with endothelial basal medium containing 0.1% FBS and 1% P/S (Thermo Fisher Scientific). For rescue experiment ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Biophysics 2023Quote: Adhered cells were labeled for plasma membrane and DNA by incubation for 1 h with CellBrite™ Green (Biotum) and 5 mg/mL Hoechst (Invitrogen), respectively ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 600 g for 5 minutes post-fixation and permeabilized with 1 mL ice-cold methanol (Fisher Scientific) for 20 minutes at 4C ...
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... and followed the same procedure described above until the application of the secondary antibody solution (5% normal goat serum in PBST, Alexa Fluor 488 anti-guinea pig 1:200 (Thermo Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... the transfected cells were blocked with 5% BSA at room temperature for 15 min and labeled with anti-FLAG antibody (1:100, Thermo Fisher) at 4°C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... and ATTO647N (Atto-Tec) were dissolved in DMSO (10 and 5 mg.mL-1, respectively) while octadecyl rhodamine B chloride (R18, Thermo Fisher Scientific) was dissolved in ethanol (10 mM).
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bioRxiv - Cell Biology 2023Quote: ... mouse calvariae were dissected from 1–3 days old neonatal mice and digested sequentially 5 times for 25 minutes in α-MEM (Gibco) containing 0.1% collagenase (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatographic separation was carried out using a Hypercarb porous graphitic carbon (PGC) column (5 µm, 150 mm x 1 mm, Thermo Scientific). A 40-minute binary gradient using 3% ACN in water containing 0.1% formic acid (Solvent A ...
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bioRxiv - Neuroscience 2024Quote: ... and NOD2−/− primary microglia treated with M18-MDP (1 μg/ml) or α-Syn PFF (5 μg/ml) were lysed in ubiquitination lysis buffer (#PI89900, ThermoFisher), supplemented with protease inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were washed with PBS three times for 5 min and incubated with secondary antibodies (goat anti-rabbit-AlexaFluor488: 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The dry pooled sample was resuspended in 1 ml of 5% formic acid and was desalted using the EasyPep Maxi Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... cells were cultured and transiently co-transfected with wild-type or mutant Nav1.2 channel variant (5 μg) and enhanced green fluorescent protein (1 μg) using Lipofectamine 3000 kit (Thermo Fisher Scientific), as previously described (26) ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then blocked with a 5% (v/v) FBS solution and incubated for 90 min in a 1:200 phalloidin–Alexa Fluor 594 (Life Technologies) diluted in a 1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... the beads were collected by centrifugation and washed with inhibitor-containing lysis buffer 5 times before being mixed with 20-40 μl 1× Laemmli sample buffer (diluted from Invitrogen, NP0008) supplemented with β-mercaptoethanol ...