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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... brains were then washed four times in 0.05% PBS-T and then incubated with 5% NGS and secondary antibody (1:400 Alexa 594 goat anti-mouse IgG, Invitrogen A11032) in 0.5% PBS-T for 2 days at 4 °C with nutation ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed and incubated with the secondary antibody dilution (Horse anti-mouse, HRP-ligated, PI 2000, 1:5000 in 5%dry milk, Thermo Fisher) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated with blocking solution (1× PBS with 5% Normal Goat Serum [50-062Z; ThermoFisher Scientific], 0.1% Triton X-100) for 15 min and with primary antibodies solutions for 2 to 3 hours at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen, G401002). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Neuroscience 2024Quote: ... Fluorescein or Texas Red labelled dextran (0.1mL of 1-5% of 70kDa dextran solution in saline; Sigma-Aldrich #46945 or Thermofisher D1830) was injected intravenously immediately before the start of imaging to visualize blood flow ...
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bioRxiv - Microbiology 2024Quote: ... and 5% O2 in complete RPMI culture medium (cRPMI) composed of iRPMI 1640 with 1% AlbuMax I (Life Technologies, Carlsbad, CA), 0.21% sodium bicarbonate (KD Medical ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was then diluted (1:5) with RNAase/DNAase-free water and qRT-PCR performed using SYBR-Green Master Mix (ThermoFisher, #4309155) and primers listed in Supplementary Data ...
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bioRxiv - Microbiology 2024Quote: ... and mounted on glass slides of 1.5 mm thickness (Fisherbrand) and covered with #1 thickness glass coverslips (Fisherbrand) using the ProLong Gold Antifade mounting medium (ThermoFisher Scientific). Images were obtained using a Nikon C2 confocal system and a Plan Apo 60X oil/1.40NA objective ...
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bioRxiv - Immunology 2024Quote: ... 1 × TE buffer (5 mM Tris base, pH 8.0 and 1 mM EDTA acid) containing 15% w/v PEG-8000 (Fisher Scientific, BP2331) and 510 mM NaCl was added to the SQB sample at 1:1 volume and mixed gently via pipetting ...
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bioRxiv - Microbiology 2024Quote: ... The parasites were washed three times for 5 min in 1 x PBS before the second antibody (Alexa Fluor 647-conjugated goat anti-Rabbit antibody (Life Technologies)) ...
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bioRxiv - Bioengineering 2023Quote: ... A total of 20 µL mixture was loaded into 384-well plate and centrifuged at 1000 rpm for 1 min before running the Melting Curve program on QuantStudio 5 Real-Time PCR System (Applied Biosystems). circRNA underwent a denaturing process at 95°C for 5 min with a ramp rate of 0.05°C/s starting from 25°C ...
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bioRxiv - Developmental Biology 2024Quote: ... manually minced and enzymatically digested in an orbital shaker for 2 hours at 37°C in digestion mix (9.2 ml DMEM/F12, 5% FCS, 1% Penicillin/Streptomycin, 25 mM HEPES (Gibco, cat. #15630056) and 300 U/ml Collagenase IV (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Glycine was then aspirated and cells permeabilised and blocked for 5 minutes with PBS containing 1% bovine serum albumin (BSA) (BP1600-100, Fisher Scientific) and 0.1% saponin (84510-100G ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3 μg of RNA was used for cDNA synthesis and 1 μl of 1:5 diluted cDNA was used in the RT-qPCR reaction triplicate (Luminaris Color HiGreen qPCR master mix, Thermo Scientific). Efficiencies of primers was calculated from standard curves to ensure similar amplification efficiencies between quantified genes ...
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bioRxiv - Immunology 2024Quote: ... major Friedlin (MHOM/SU/73/5-ASKH) strain (1×105 logarithmic promastigotes per mL) were cultured at 26 °C in M199 medium (Gibco, USA) (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed using RIPA lysis buffer (0.1% SDS, BP8200100, Fisher Scientific, 1% IPEGAL CA630, 8896, EMD-Millipore; 0.5% sodium deoxycholate, D6750, Sigma, 50mM Tris, BP152-5, Fisher Scientific; 150mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed with TBS-T buffer and blocked with either 5% milk diluted in TBS-T or 1% casein in TBS (ThermoFisher: 37532) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted fragment solution was then directly injected at a rate of 5 μL min-1 into the Q-exactive Plus Mass Spectrometer (Thermo Scientific) and measured in negative mode ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time PCR was performed in triplicates using 1 µl cDNA and 9 µl master mix consisting of 5 µl SYBR Green PCR Mix (4309155, Applied Biosystems), 3 µl H2O and 1 µl of forward and reverse primers (0.5 µM final concentration) ...
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bioRxiv - Cancer Biology 2024Quote: ... while a global protein aliquot was resuspended in 50 μl of 0.1% FA and 5 µl was loaded twice onto a Dionex U3000 RSLC in front of an Orbitrap Eclipse (Thermo Fisher) equipped with an EasySpray ion source ...
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bioRxiv - Cell Biology 2024Quote: ... cells were cultured for 72 h in RPMI 1640 medium supplemented with 1% pen-strep and 5% exosome-depleted FBS (GibcoTM, ThermoFisher Scientific). sEV derived from A549 cells were isolated from the conditioned medium by differential ultracentrifugation ...
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bioRxiv - Immunology 2023Quote: ... in PBS for 1 h at 25°C followed by permeabilization in 5% BSA in the presence of 0.05% Tween-20 (Thermo Fisher Scientific) for 15 minutes at 25°C ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed three times for 5 min in TBS-T at room temperature and incubated in 1:10000 secondary anti-Rabbit-HRP (Invitrogen, #31460) in TBS-T/Milk 5% for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained in phosphate buffered saline containing 1 μg/mL PI and 5 μg/mL Hoechst 33342 (Thermo Fisher Scientific) and the average number of PI- live cells per condition was determined using a CellInsight CX5 High Content Screening Platform ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% Penicillin Streptomycin at 37 °C and 5% CO2 and split when confluent using Trypsin-EDTA (0.05%) (Life Technologies, 25300054). When indicated ...
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bioRxiv - Biochemistry 2023Quote: ... washed twice in DMEM with 10% CCS and suspended in Williams E medium supplemented with 5 % CCS and 1 mM glutamine (Invitrogen, CA). To separate live from dead cells ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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bioRxiv - Microbiology 2023Quote: ... 1X MOPS (20 mM MOPS, 5 mM NaOAc, 1 mM EDTA, pH 7.0) and 1X RNA loading dye (Thermo Fisher Scientific) for 10 min at 70°C and incubated on ice ...
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bioRxiv - Microbiology 2023Quote: ... The azide group of ADA-DA was labelled (post-fixation) with 5 µg ml−1 Alexa Fluor 488 Alkyne using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen) according to the manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... A 1:5 dilution of cDNA and custom primers were input into qPCR reactions with PowerUP SYBR Green Master Mix (Applied Biosystems).qRT-PCR was performed using a QuantStudio 3 Real Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Serial 10-fold dilutions of gene fragments were performed in sheared salmon sperm DNA 5 µg mL-1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by a 5 min incubation with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) and revealed using a Chemidoc MP Immaging System (BIO-RAD) ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged every 5-6 days as small clumps by dissociation with a buffer containing 1 mg per ml Collagenase IV (ThermoFisher Scientific), 0.025% Trypsin (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were washed 3 times for 5 min with PBTx before incubation at room temperature in 1 μg/mL DAPI (Invitrogen, ThermoFisher Scientific) solution in PBS for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... 2.5 µl of a 200 nM stock pool of each primer pair (Supplementary File 1) was added to 5 µl of TaqMan PreAmp Master Mix (Applied Biosystems) and 2.5 µl of 1:5 dilution of cDNA ...
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bioRxiv - Biochemistry 2022Quote: ... Labeling was performed by the addition of dyes at a molar ratio of 5:1 dye:nanobody using Alexa 488 NHS (Thermo Fisher Scientific) for the GFP nanobody and Alexa 568 NHS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse transcribed and amplified in 5 reactions with 1 µg RNA template using SuperScript IV One-Step RT-PCR System with ezDNase (Invitrogen, 12595025) and the primers ...
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bioRxiv - Cancer Biology 2023Quote: For sEVs isolation cells were cultured for 72 h in RPMI 1640 medium supplemented with 1% pen-strep and 5% exosome-depleted FBS (GibcoTM, ThermoFisher Scientific). sEV were isolated from the conditioned medium (CM ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 mg/ml fibrinogen and 0.2% LAP for photoinitiation and cultivated in vitro for up to 5 days with endothelial basal medium containing 0.1% FBS and 1% P/S (Thermo Fisher Scientific). For rescue experiment ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Biophysics 2023Quote: Adhered cells were labeled for plasma membrane and DNA by incubation for 1 h with CellBrite™ Green (Biotum) and 5 mg/mL Hoechst (Invitrogen), respectively ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 600 g for 5 minutes post-fixation and permeabilized with 1 mL ice-cold methanol (Fisher Scientific) for 20 minutes at 4C ...
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... and followed the same procedure described above until the application of the secondary antibody solution (5% normal goat serum in PBST, Alexa Fluor 488 anti-guinea pig 1:200 (Thermo Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... the transfected cells were blocked with 5% BSA at room temperature for 15 min and labeled with anti-FLAG antibody (1:100, Thermo Fisher) at 4°C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... and ATTO647N (Atto-Tec) were dissolved in DMSO (10 and 5 mg.mL-1, respectively) while octadecyl rhodamine B chloride (R18, Thermo Fisher Scientific) was dissolved in ethanol (10 mM).
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bioRxiv - Cancer Biology 2023Quote: ... The tops of the micropillars were coated with a mixture of unlabeled and Alexa Fluor 647-labeled fibronectin (1:5, Life Technologies) using micro-contact printing ...
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bioRxiv - Biophysics 2023Quote: ... 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio) were mixed with 15 ml FreeStyle 293 media (Thermo Fisher) and 1.2 ml of PEI (Polysciences ...