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Citations for Thermo Fisher :
2151 - 2200 of 3952 citations for Rubella Virus VLP strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Vero FM cells were infected with passage 3 stock at an MOI of 0.01 in virus infection medium [DMEM (Gibco) containing 2% FCS (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Immunology 2020Quote: ... and Covid-19 virus detection was performed using One-Step qRT-PCR SuperScript™ III Platinum™ (Life Technologies) targeting the gene E ...
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bioRxiv - Microbiology 2021Quote: ... Fibroblasts were reprogrammed into iPSCs by using the episomal Sendai virus approach (CytoTune-iPS 1.0 Sendai Kit, Life Technologies) to obtain hiPSCs ...
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bioRxiv - Immunology 2022Quote: ... according to manufacturer’s recommendation and qRT-PCR was performed using TaqMan Fast Virus 1-Step Mix (Applied Biosystems, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Viral stocks were passaged and expanded using MDCK-II cells in virus growth medium comprised of Opti-MEM (Gibco), 2.5 mg/mL bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... and then quantified by qRT-PCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with the TaqMan™ 2019nCoV Assay Kit v1 (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
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bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was removed 16-20 hours following transduction with fresh neural media [Neurobasal medium (NBM; Life Technologies, #21103-049) supplemented with 1x N-2 supplement 1x B-27 supplement ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A virus growth medium consisting of infection media supplemented with an additional 1 µg/mL trypsin-TPCK (ThermoFisher, USA) was added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and virus pellet was resuspended in the same volume of RIPA buffer (Cat #89901, ThermoFisher, 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Moloney murine leukemia virus (M-MLV) reverse transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor were purchased from Invitrogen (Carlsbad, CA). The Phospho-Akt (Ser473 ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin used for virus propagation was standard cell culture grade 0.25% porcine trypsin with EDTA and phenol red (ThermoFisher). Huh-7 cells were seeded on a 24-well plate and incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: The RNA of H1N1pdm09 was extracted from virus samples using a PureLink™ viral RNA/DNA mini kit (Invitrogen). For qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were prepared by three passages in Vero (ATCC CCL-81) in Dulbecco’s modified Eagle Medium (DMEM) (Gibco) with 1% Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... Viral plaques were passaged at an MOI of ∼0.001 in MDCK-II cells in virus growth medium comprised of Opti-MEM (Gibco), 2.5 mg/ml bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells infected with pLENTI-Luciferase-expressing virus (generous gift of Charlotte Kuperwasser, Tufts University) were selected with neomycin (G418, 500μg/mL, Gibco) for 2-3 weeks ...
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bioRxiv - Biophysics 2023Quote: ... Negatively stained virus particles were observed on a 30 kV scanning electron microscope (Thermo Fisher Scientific, Quanta FEG 650) in scanning transmission electron microscopy (STEM ...
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bioRxiv - Cell Biology 2023Quote: ... HT914 and HT915 iPSC lines) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) as previously reported [43–45] ...
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bioRxiv - Microbiology 2023Quote: ... The RT-PCR was performed using the Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a QuantStudio3 (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... MED1-IDR containing plasmid was obtained from Addgene #145276 and inserted into pcDNATM6.2-C-EmGFP-DEST supplemented with the Simian Virus 40 NLS sequence by Gateway strategy (Invitrogen) using primers described in Suppl ...
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bioRxiv - Microbiology 2023Quote: ... MRC-University of Glasgow Centre for Virus Research) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher, 10566016) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: The virus riboswitch (vRibo) RNAs were synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific Cat# K0441) from the DNA templates generated by PCR ...
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bioRxiv - Microbiology 2024Quote: ... The first strand cDNA was obtained using oligo(dT) primers and Moloney murine leukaemia virus-reverse transcriptase (Life Technologies), using 100 ng of purified RNA ...
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bioRxiv - Physiology 2024Quote: ... and infected with sendai virus (SeV) carried polycistronic Klf4-Oct3/4-Sox2 and Klf4 vectors (ThermoFisher, Waltham, MA, #A16517), here referred as OSK-SeV ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we extracted virus from the pooled samples from each assay grid using a sterile pellet pestle (Fisher Scientific, U.S.A.) to manually burst larvae and release viral occlusion bodies ...
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bioRxiv - Cell Biology 2024Quote: ... the virus titer was quantified using quantitative polymerase chain reaction (qPCR) with SYBR Green Master Mix (Thermo Scientific, 4309155) and primers targeting ITRs (Supplementary Table 1) ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted from the supernatant of MDCK cells infected with the PB2 TRAP V5 virus using Trizol (Invitrogen). RNA was reverse-transcribed with Superscript III (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... at a 6:1:2 ratio for each virus using the Bac-to-Bac baculovirus expression system (Thermo Fisher). Cell cultures were grown in ESF 921 medium (Expression Systems ...
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bioRxiv - Immunology 2024Quote: ... RNA was then reverse transcribed from 200 ng total RNA with Moloney murine leukemia virus reverse transcriptase (Invitrogen #28025013) using oligo (dT)18 (Thermo Scientific #SO131 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with N2 (made in-house 2x stock in Advanced DMEM/F-12 (Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was replaced with culturing medium (Ham’s F-10 (Cellgro) containing 10% horse serum (Invitrogen), 10% FBS (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... A549 cells were purchased from ATCC and cultured in Ham’s F-12K medium (Thermo Fisher, 21127022). Human induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs ...
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bioRxiv - Cell Biology 2020Quote: ... and hTERT-RPE-1 Cyclin A2-eYFP54 were cultured with DMEM/F-12 + GlutaMax (Life Technologies), and BJ primary human foreskin fibroblasts (a kind gift from Bennie Lemmens ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured in DMEM/F-12 supplemented with 2.5 mM L-glutamine (Gibco, Carlsbad, CA, USA) and 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... and Adgrg6 KO ATDC5 cells were maintained in DMEM/F-12 (1:1) medium (Gibco, 11330032) supplemented with 5% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: FreeStyle 293-F (cat# R79007) and Expi293F cells (cat# A14528; RRID: CVCL_D615) were purchased from ThermoFisher Scientific Inc ...
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bioRxiv - Physiology 2019Quote: ... qPCR was performed using the DyNAmo HS SYBR Green qPCR Master Mix (Fisher Scientific, F-410). All samples were run in triplicate reactions ...
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bioRxiv - Molecular Biology 2019Quote: ... Base media for mESCs was made with DMEM/F-12 lacking Lysine and Arginine (Life Technologies) plus all additional components required for mESC culture as listed above ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... F(ab)’ fragment affinity-purified unconjugated goat anti-mouse IgG (Thermo Fisher Scientific; 1.25 μg/ml) and goat anti-mouse IgG (H&L ...
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bioRxiv - Cell Biology 2019Quote: ... goat anti-mouse Alexa Fluor 488 F(ab’)2 Fragment (2 μg/ml, A11017; Molecular Probes), Alexa Fluor 568–phalloidin (1:200 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12; Gibco) supplemented with 10% fetal bovine serum (Gibco) ...