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Citations for Thermo Fisher :
2301 - 2350 of 3952 citations for Rubella Virus VLP strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... perRB (M1474) and double perRAperRB mutant strains were harvested and resuspended in 1 ml TRIzol (ThermoFisher Scientific) and stored at -80°C ...
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bioRxiv - Molecular Biology 2021Quote: Cells from each strain and condition were first resuspended in 0.5 ml TRI reagent each (ThermoFisher, AM9738). One aliquot of frozen S ...
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bioRxiv - Microbiology 2019Quote: ... The strain was also incubated under anaerobic conditions in a Forma Anaerobic System Glovebox (Thermo Scientific, 1025). The growth pattern of the isolate was also determined on Salmonella-Shigella Agar plate (Himedia ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid construct was transformed into an electrocompetent strain of Escherichia coli DH10β cells (ThermoFisher Scientific, USA) via electroporation ...
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bioRxiv - Biochemistry 2021Quote: The DNA sequence encoding the TBEV NS2B-NS3pro enzyme (strain Hypr; GeneBank: KP716978.1) was commercially synthesized (Invitrogen) and encoded NS2B (residues 45-96) ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae serotype 2 strain D39 [17] was plated on brain heart infusion (BHI, Thermo Scientific, MA, USA) agar plates supplemented with 3% v/v defibrinated horse blood (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... All HTM cell strains were validated with dexamethasone-induced (DEX; Fisher Scientific, Waltham, MA, USA; 100 nM) myocilin expression in more than 50% of cells by immunocytochemistry and immunoblot analyses (Suppl ...
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bioRxiv - Genetics 2022Quote: ... both strains were harvested at an OD600 of 0.3 pelleted and immediately suspended in RNAlater (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: The H99S strain [21] of Cn serotype A was grown in yeast peptone dextrose (YPD; ThermoFisher Scientific) broth ...
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bioRxiv - Biochemistry 2019Quote: ... smegmatis wild-type or Rv1626D65E over-expression strain was used for DNase I (AM2238, Thermo Fisher Scientific) treatment ...
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bioRxiv - Synthetic Biology 2020Quote: Promastigote-form of Leishmania donovani (Ag83 strain) was cultured at 26 *C in M199 media (GIBCO, India) pH 7.4 supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
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bioRxiv - Microbiology 2020Quote: Approximately 2.8 × 107 cells/ml of Escherichia coli strain DH10B harboring a pBAD vector (Thermofisher Scientific, Canada), suspended in a total of 50 ml 1× PBS ...
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bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
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bioRxiv - Immunology 2021Quote: ... aeruginosa (strain PA14) and clinical isolates were grown in lysogeny broth (LB, Thermo Fisher Scientific, Waltham, MA) liquid cultures at 37°C with shaking at 225 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... Yeast strains used in Tables S1-S2-S3 were taken from the heterozygote yeast deletion collection (ThermoFisher). For nicotinamide (NAM ...
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bioRxiv - Microbiology 2022Quote: Spn serotype 4 strain TIGR4 was grown overnight on 1% agar- Todd Hewitt Broth (Thermo Fisher Scientific) with yeast (THY ...
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bioRxiv - Molecular Biology 2022Quote: ... falciparum parasite strains (3D7, NF54, R539T, and RKL-9) were cultured in RPMI 1640 media (Gibco, USA) supplemented with 50 mg/L hypoxanthine (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... total RNA was extracted from wild type zebrafish larvae (AB strain) through TRIzol RNA-extraction (Invitrogen, 10296010) and cDNA synthesis was performed using iScript Reverse Transcription SuperMix (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... donovani (strain BPK190, (Decuypere et al., 2005)) were grown at 28°C in M199 medium (Life Technologies) supplemented with 2.2 g/L NaHCO3 ...
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bioRxiv - Microbiology 2024Quote: ... pneumoniae strains to a range of antibiotics was determined using broth microdilution (Trek Sensititre®, ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: MDCK strain II cells were cultured in minimum essential medium with Earle’s Salts and l-glutamine (Gibco) supplemented with 10% v/v foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... infantum (strain MHOM/FR/91/LEM2259, (40)) were grown at 28°C in M199 medium (Life Technologies) supplemented with 2.2 g/L NaHCO3 ...
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bioRxiv - Microbiology 2023Quote: ... or Ara-YenR ΔYeIspn/YeOspn strain cells were clipped in cryo-FIB-specific AutoGrids (Thermo Fisher Scientific) with alignment markers and a cut-out for milling at shallow angles ...
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bioRxiv - Microbiology 2023Quote: ... Parasites (CDC1132 or G3 strain) were labelled with Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin) (Invitrogen), added to confluent BPH-1 cells (1:3 parasite ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A-tagged strains were immunoprecipitated with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) (Vakiloroayaei et al. ...
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bioRxiv - Microbiology 2023Quote: ... nucleatum strains ATCC 23726 and 25586 were cultured on solid agar plates made with Columbia Broth (Gibco) substituted with hemin (5 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... coli strains with one of the following kits: GeneJet plasmid miniprep kit (catalog number K0503; Thermo Scientific). QIAprep Spin Miniprep Kit (catalog number 27104 ...
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bioRxiv - Microbiology 2023Quote: ... aureus strains were grown in modified M63 medium to mid-logarithmic phase and then WGA-488 (Invitrogen) was added to 1 μg ml-1 final concentration and incubated for 5 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence (excitation 512 nm, emission 527 nm) of each strain (200 µl – Nunc black well plates) was read in triplicate using an Ensight multimode plate reader (PerkinElmer ...
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bioRxiv - Plant Biology 2023Quote: ... The product of recombination reactions (BP reactions) was used to transform competent Escherichia coli strain TOP10 (Invitrogen), by heat shock ...
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bioRxiv - Molecular Biology 2023Quote: ... AJY4660 and AJY4661 were made by integrating PmeI-cut pAOL47-04 into the respective GFP strains (Invitrogen). AJY4654 was made by integrating PmeI-cut pAOL47-04 into AJY4047 ...
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bioRxiv - Molecular Biology 2023Quote: ... AJY3027 was constructed by sequential crossing appropriate haploid strains originally derived from the heterozygous deletion collection (Invitrogen). The arx1 deletion allele was converted from KanMX to NatMX by homologous replacement using p433939 ...
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bioRxiv - Microbiology 2024Quote: ... falciparum strains were cultured at 37°C in RPMI 1640 medium supplemented with 0.5% Albumax II (Gibco) (Trager and Jensen 1976) ...
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bioRxiv - Microbiology 2024Quote: ... parviporum strains (Supplemental Table S1) were treated with the TURBO DNA-free™ Kit (Thermo Fisher Scientific) and sent to SEQme s.r.o ...
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bioRxiv - Microbiology 2020Quote: ... five-fold serial dilutions of each virus stocks were prepared in a separate 96-well plate in Opti-MEM (Gibco). Medium in the 96-well plate was removed and the cells were washed with 100 µL of warm PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... the plate was checked for confluence and the cells were infected with serial dilution of virus in OptiPro serum free media (Thermofisher) with 1% L-Glutamin (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μl of RNAse-Out and 1 μl of the Moloney Murine Leukemia Virus reverse transcriptase enzyme (MML-V, Invitrogen). Quantitative PCR was carried out in a total of 15μl consisting of 3μl cDNA ...
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bioRxiv - Plant Biology 2020Quote: ... NAA50 was cloned into the cauliflower mosaic virus 35S promoter vector pEarleyGate100 (Earley et al. 2006) using a modified multisite Gateway recombination cloning system (Invitrogen) as described in (Qi et al ...
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bioRxiv - Developmental Biology 2019Quote: Pure cultures of 10 × 104 prostate stromal cells seeded on twelve well plates were transduced using Cytotune 2.0 Sendai Virus reprogramming vectors (KOS, c-Myc and Klf4, Life Technologies) at a multiplicity of infection of 5-5-3 (KOS MOI=5 ...
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bioRxiv - Microbiology 2019Quote: ... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
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bioRxiv - Cell Biology 2019Quote: ... and HY03 were reprogrammed using the Sendai virus and the CytoTune®-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), and display all the PSC features ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with PBS to remove residual input virus and overlaid with DMEM with glutamine (Life Technologies) with 0.7% IgG-free BSA (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... Untransduced cells and cells treated with the serially diluted virus were both cultured in the presence of 2 µg/ml puromycin (Invitrogen). The lentiviral titer was quantified by counting the number of living cells at the time when all of the untransduced cells died as previously described26.
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bioRxiv - Biochemistry 2020Quote: ... The plasmid was linearized with XbaI and in vitro transcribed into the genomic RNA of DENV-Luc virus using the T7 Megascript Kit (Ambion) in the presence of m7G(5’)ppp(5’)G RNA Cap Structure Analog (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... The virus particles were then harvested 24 to 48 hours later by passing through a 0.45 µm syringe filter (Thermo Scientific). Viruses were mixed with an equal volume of fresh media supplemented with 10% FBS and polybrene was added at a final concentration of 5 µg/mL to increase infection efficiency ...
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bioRxiv - Neuroscience 2021Quote: ... Cell reprogramming was performed using the Sendai virus system with SOX2/OCT4/KLF4/MYC (CytoTune-iPS Reprogramming Kit; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... by the acid guanidinium/phenol/chloroform method using ISOGEN (Nippon Gene) was converted to cDNA using Moloney murine leukemia virus reverse transcription (Invitrogen) and oligo-dT primers ...
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bioRxiv - Bioengineering 2021Quote: ... a pure DI244 seed virus at MODIP 1E–3 and 20 U/mL trypsin (5000 U/mL in PBS; 27250018, Gibco) were added ...