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Citations for Thermo Fisher :
2401 - 2450 of 3952 citations for Rubella Virus VLP strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Virus propagation and titration was performed in LLC-MK2 cells using Opti-MEM supplemented with 1.6% TrypLE™ Express (Gibco) and 100 μg/ml CaCl2 (OptiMEMinf ...
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bioRxiv - Plant Biology 2022Quote: ... was transcribed following DNase I treatment using Moloney Murine Leukemia Virus reverse transcriptase and an oligo dT(18) primer according to the manufacturer’s protocol (ThermoFisher Scientific). qPCR analysis was carried out in a CFX96-C1000 96-well plate thermocycler (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg of total RNA was subjected to random reverse transcription using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT; Invitrogen) and random primers (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... GH3 cells were transduced with supernatants containing virus carrying the shMeCP2 or shhnRNP L construct using the Lipofectamine 3000 reagent (Invitrogen). After 48 h ...
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bioRxiv - Neuroscience 2024Quote: ... PBMCs were reprogrammed into iPS cells using transient overexpression of pluripotency-associated factors (OCT4, KLF4, and SOX2) via non-integrating Sendai virus particles (Cytotune 2.0 kit, Life Technologies). At least five clones per TSC2+/+ and TSC2+/− donor were established into iPS cell lines ...
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bioRxiv - Genetics 2024Quote: ... The iPSCs were made Sendai virus free by keeping in culture and testing for the presence of Sendai Virus (SeV) at different times by measuring SeV levels in total RNA using RT-qPCR with FAM labeled SeV (Thermofisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription with random hexamer as the primer (SuperScript III, ThermoFisher Scientific). The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase ...
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bioRxiv - Synthetic Biology 2023Quote: ... The virus concentration using Lenti-X Concentrator was performed with the manufacturer’s protocol and dissolved in Opti-MEM (Gibco #31985062) to yield a 10- or 15-fold concentration ...
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bioRxiv - Microbiology 2023Quote: ... generated by Knut Falk in the late 1990s) or mouse IgG2a targeting influenza virus NP (clone D67J, Thermo Fisher Scientific) (both ...
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bioRxiv - Developmental Biology 2023Quote: ... PD2 and TD1 hiPSC were generated from deidentified commercially available human peripheral blood mononuclear cell and tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (ThermoFisher, A16517). Every other month ...
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bioRxiv - Microbiology 2023Quote: BmN cells infected with WT virus at indicated time were harvested and total RNA was extracted by using TRIzol reagent (Invitrogen). 2 μg total RNA was prepared to synthesis the cDNA by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen) ...
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bioRxiv - Microbiology 2023Quote: ... The following day we washed cells with DPBS twice and added half-log10 dilutions of the virus sample in infection media (MEM medium (Gibco) containing 0.3% BSA (fraction V ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed according to the manufacturer’s protocol using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher). Fluorescent signals were acquired using QuantStudio 6 Real-time PCR system (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... Lungs from K18-ACE2 C57BL/6 mice infected with virus were collected at indicated time points and were homogenized in Trizol (Invitrogen) and RNA was isolated using manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Total RNA was extracted from clinical specimens (swabs and tissues) and virus isolates using the MagMax AM1836 96 Viral RNA Isolation Kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT) (Invitrogen).
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bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...
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bioRxiv - Biochemistry 2023Quote: ... Concentrated virus was applied through a 0.45 μm surfactant free cellulose acetate syringe filter (Nalgene, Fisher Scientific #09-740-106) and immediately used or stored at -80ºC for up to one month ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed with 1 µg of RNA using 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to instruction of the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... the fibroblasts were thawed and plated at a density of 2.5×105 cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Cell Biology 2024Quote: ... in this study were generated from deidentified commercially available human tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (Thermo Fisher) as described before24 ...
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bioRxiv - Cell Biology 2024Quote: We established mouse iPSC lines (CD1 iPSC or ntdTomato iPSC) using Sendai virus-mediated reprogramming kit: CytoTune2.0 (Thermo Fisher, A16517) and followed manufactured protocol as described before24 ...
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bioRxiv - Neuroscience 2024Quote: ... sections of virus-injected mice were incubated in a solution of chicken anti-eGFP (1:2000, Thermo Fisher Scientific, A10262) and mouse anti-gephyrin (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Biochemistry 2024Quote: ... Ten micrograms of total RNA was reverse transcribed (RT) at 37 °C using random hexamer primers and Moloney murine leukemia virus retrotranscriptase (Invitrogen-Life Technologies ...
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bioRxiv - Microbiology 2024Quote: Virus-infected cells and the supernatants were dissolved with Tris-glycine SDS sample buffer (Thermo Fisher Scientific, Waltham, MA, USA), boiled for 5 min in the absence of reducing agent ...
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bioRxiv - Cell Biology 2024Quote: ... were co-transfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using Lipofectamine 3000 (Cat# L3000001, ThermoFisher). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplification was performed using Phusion High-Fidelity DNA Polymerase (2 U/µl; Thermo Fisher, F-530L). For this 2 μl of cDNA were mixed with 10 μl of HF buffer ...
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bioRxiv - Genomics 2021Quote: ... Cells were then treated with APC-conjugated F(ab’)2-goat anti-mouse secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... Tissues were then washed and plated with DMEM/F-12 medium containing 15% fetal bovine serum (GIBCO) and Antibiotic Antimycin (GIBCO) ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel droplets were overlaid with recombinant organoid medium (Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used were F(ab’)2 goat anti-rabbit Alexa Fluor 568 (Molecular Probes, 1:1000), goat anti-mouse Alexa Fluor 488 (Molecular Probes 1:1000).
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 0.03% Pluronic F-127 and 0.6 μM cell-permeable form of indicator (Fluo4-AM, Invitrogen) for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The beads were washed again with PBS using magnets and resuspended in 5% Pluronic F-68 (Gibco) PBS solution and incubated for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... primary GBM cells were cultured in DMEM/F-12 medium (Thermo Fisher Scientific, Gibco, #8117165, Shanghai, China) supplemented with B27 (1× ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were incubated with 5 μM Fura-2 AM and 0.04% Pluronic F-127 (Thermo Fisher Scientific) for 45-60 min at 37 °C in standard extracellular solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary NP cells were cultured with growth medium (Dulbecco’sModified Eagle’s Medium and Ham’s F-12 Nutrient Mixture (DMEM-F12; Gibco), 20% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Biopsy tissues are transported in 50ml conical tubes containing collection media (Advanced DMEM/F-12 (Invitrogen 12634028) supplemented with 2 mM GlutaMax ...
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bioRxiv - Developmental Biology 2019Quote: ... tracheas were dissected in ice-cold Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (ThermoFisher, DMEM/F12), and sliced open along the proximal-distal axis prior to imaging ...
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bioRxiv - Biophysics 2019Quote: ... tracheas were dissected in ice-cold Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (ThermoFisher, DMEM/F12), and sliced open along the proximal-distal axis ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... Cellular DNA and F-actin was then counterstained with DAPI and Alexa Fluor 647 Phalloidin (Thermo Fisher). Image acquisition and quantitative analysis were performed as described above.
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bioRxiv - Microbiology 2019Quote: ... ATCC no: CCL-61) were maintained in Kaighn’s modification of Ham’s F-12K nutrient mixture (Life Technologies). All cells were supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2019Quote: ... The normal cell culture medium is made from Advance DMEM/F-12 culture medium (A2494301, ThermoFisher scientific) supplemented with 5% fetal bovine serum (12676011 ...
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bioRxiv - Molecular Biology 2019Quote: ... UPCI-SCC154 (ATCC) cells were cultured on Collagen I coated plates in DMEM/F-12 (Gibco, 11320082) supplemented with GlutaMAX (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... F-actin and nuclei were stained with phalloidin conjugated to an Alexa Fluor 488 fluorophore (Life Technologies) and 4’,6-diamidino-2-phenylindole ...
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bioRxiv - Microbiology 2021Quote: ... in complete neural stem cell (CNSC) medium containing Knock-out D-MEM/F-12 (A1370801, Thermo Fisher), 2 mM Glutamax (35050038 ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM F12, Gibco, ThermoFisher Scientific) supplemented with 5ml streptomycin-penicillin (10,000 U/mL ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM F12, Gibco, ThermoFisher Scientific) supplemented with 5ml streptomycin-penicillin (10,000 U/mL ...