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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... thermoblocks at 90°C and 50°C (e.g., Thermo Fisher, 88870004), an ultracentrifuge capable of spinning 50 ml falcon tubes at 10,000 rpm (Beckman Coulter Avanti J-30 I ultracentrifuge and a Beckman JA-12 fixed angle rotor) ...
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bioRxiv - Neuroscience 2024Quote: ... Fast SYBR™C Green Master Mix (Applied Biosystems™C) was employed along with primers at a concentration of 5nM ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide separation was performed by an EASY-Spray column at 45 °C (50 cm × 75 µm ID, PepMap RSLC C18, 2 µm; Thermo Fisher Scientific). A 30 min linear 8-30% [vol/vol] ACN gradient was used with a flow rate of 200 nl/min ...
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bioRxiv - Genetics 2021Quote: ... at 85 V for 2 hours 15 minutes at an ambient temperature of 4 °C in NuPAGE™ Transfer Buffer (Invitrogen, UK) containing 10% 2-propanol (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and the extracted RNA was precipitated at −20°C for 2 h in 300 μl absolute ethanol with 1μl Glycoblue (Invitrogen, cat. No. AM9516). The RNA was pelleted in a chilled microcentrifuge at maximum speed for 10 min and washed with 500 μl 70% ethanol ...
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bioRxiv - Epidemiology 2019Quote: ... for 2 h at room temperature and incubated overnight at 4°C with rabbit polyclonal anti-myeloperoxidase (MPO, 1:100, Invitrogen, CA, USA) or rabbit monoclonal anti-F4/80 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Embryo hippocampi were dissected in 4 °C Hibernate E supplemented with 2% B27 supplements and 100 U/mL Penicillin/Strep (Thermo Fisher Scientific). Hippocampal tissues were digested in Hibernate E containing 20 U/mL papain ...
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bioRxiv - Cell Biology 2020Quote: ... For transient overexpression (Fig 2 E-H, S2G) seipin tagged with C-terminal EGFP [34] was inserted into pcDNA5/FRT/TO (Thermo Fisher Scientific) through HindIII/NotI sites ...
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bioRxiv - Neuroscience 2021Quote: ... hMOs were then washed overnight with 1X PBS at 4°C and incubated with Hoechst 33342 solution in 1X PBS for 2 h (1:1000, Thermo Fisher Scientific) and washed in PBS for 2 h ...
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bioRxiv - Cancer Biology 2019Quote: ... washed extensively with phenol red-free RPMI containing 2% FBS and stored overnight at 4°C prior to staining with CellEvent Senescence Green (Thermo Fisher Scientific). Flow cytometric analysis and sorting was performed at the Flow Cytometry shared Resource Facility at the Icahn School of Medicine at Mount Sinai ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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bioRxiv - Biochemistry 2021Quote: ... Grids were then blotted with filter paper for 2 s at 100 % humidity at 4 °C and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: The ubiquitylated worm CMG was then incubated for 30 minutes at 4°C with 2 µl (per 10 µl ubiquitylation reaction mix) magnetic beads (Dynabeads M-270 Epoxy; Life Technologies, 14302D) that had been coupled to anti-worm SLD-5 antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The peptides were injected into a reverse phase C-18 column (PepMap RSLC C18, 2 μm, 100 Å, 75 μm × 50 cm, Thermofisher Scientific) and separated by a gradient flow of solvent B (0.1% formic acid in 80/20 acetonitrile/water ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove the excess of primary antibody and incubated with the appropriate Alexa-Fluor secondary antibodies for 2 h at 25 °C (1:1000, Thermo Fisher Scientific). The excess of secondary antibody was washed ...
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bioRxiv - Microbiology 2020Quote: ... cells or tissue explants were incubated with virus (MOI 0.05-5) for 1-2 hr or overnight respectively at 37°C using fresh media supplemented with fetal bovine serum (Thermo Fisher Scientific). For co-infection assays ...
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bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were kept at 37°C and 5% CO2 in neurobasal medium containing 2 mM L-glutamine and B27 supplement (Thermo Fisher Scientific) with an exchange of half the medium after 4 days in culture.
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bioRxiv - Cancer Biology 2021Quote: ... and a heated (50 °C) reversed-phase analytical column (Acclaim PepMap RSLC C18, 2 µm, 100 Å, 75 µm × 150 mm, nanoViper, Thermo Scientific) coupled online with the MS ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were maintained at 37°C in a 5% CO2 humidified atmosphere and were passaged every 2 days by dissociation with TrypLE (Thermo Fisher Scientific). Culture media was replaced with fresh stocks daily ...
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bioRxiv - Cell Biology 2022Quote: ... 45 s in air at 15 mA and 0.4 mbar) holey carbon grid (C-flat: 2/1-3C-T) and vitrified using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) after blotting for 3 seconds with blot force 18 ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were lysed in a hot (60 °C) phenol solution (2 mM EDTA, pH8, 1% SDS, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) ...
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bioRxiv - Microbiology 2019Quote: ... The prehybridization (1-2 h) and hybridization (16 h) steps were performed at 37°C using NorthernMax® prehybridization and hybridization buffers (LifeTech, Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2019Quote: ... The agarose plugs were washed with Tris-EDTA buffer the following day and digested at 43°C with GELase™ Agarose Gel-Digesting Prep (2 unit/µl, Thermo Fisher) for 50 min ...
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bioRxiv - Immunology 2019Quote: ... 5×106 cells/ml PBMC were incubated at 37°C for 18 h or 2×106 cells/ml PBMC for 5 days in RPMI 1640 GlutaMAX medium (Life Technologies/Invitrogen) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: The expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI-(Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were then separated on a 45°C heated EASY-Spray column (50 cm × 75 µm ID, PepMap RSLC C18, 2 µm; Thermo Fisher Scientific) using a 3 h linear 8-30% (vol/vol ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Genomics 2020Quote: ... was reverse transcribed for 50 min at 50°C using 50 μM oligo dT and 2 μl of SuperScript III (Thermo Fisher Scientific) in a 20 μl reaction ...
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bioRxiv - Microbiology 2021Quote: Three µL of purified virus particles were loaded on a glow-charged lacey carbon copper grid and flash frozen in liquid ethane after 2 s blotting in 100% humidity at 4°C using a Vitrobot Mark IV (Thermo Fisher Scientific). The particles images were collected using a 300 kV Titan Krios G2 microscope (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times with PBS and incubated for 2 h at 4°C with appropriate combinations of AlexaFluor-conjugated secondary antibodies (Invitrogen,1:2000) for 2 hours at 4C protected from light ...
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bioRxiv - Microbiology 2022Quote: ... the expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI- (Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Neuroscience 2022Quote: The intracellular Ca2+ concentration was assayed for 20 min at 37°C with 2 µM Fluo-4-AM (catalog number F14201, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of lysates were incubated with anti-53BP1 for 2 hours at 4°C and subsequently with Protein A magnetic beads (Thermo Fisher Scientific) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells (CCL-2; ATCC) were cultured at 37°C and 5% CO2 in DMEM/high-glucose/GlutaMAX medium (10564029; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C to an A600 of 0.6 was used to inoculate 2 liters of Terrific Broth (Fisher Scientific, cat. no. 22711022) supplemented with 100 μg/ml ampicillin ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were then resolved on a 50 cm × 75 μm C18 analytical column with integrated electrospray emitter heated to 40 °C (Easy-SPRAY, 2 μm, 100 Å, Thermo Scientific) using the following gradient at a flow rate of 250 nL/min ...
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bioRxiv - Microbiology 2022Quote: ... and treated during 30 minutes at 37°C with 4 µg of RNAse and 2 U of Turbo DNAse (Ambion, Ref AM2239). Another incubation of 30 minutes at 37°C with an additional quantity of Turbo DNAse (2 U ...
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bioRxiv - Neuroscience 2024Quote: ... Joachim Herz) for 2 h at 4°C followed by overnight incubation with 20 μL of protein G Ultralink resin (ThermoFisher Cat# 53128). The resins were washed with immunoprecipitation buffer and precipitated proteins were eluted with reducing SDS sample buffer ...
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bioRxiv - Physiology 2024Quote: ... Coverslips with attached cells were incubated for 30 min at 37 °C in ES containing 2 µM Calcium Green-1 AM (Invitrogen C3011MP, Thermo Fisher Scientific) and 0.02% Pluronic F-127 (Invitrogen P6867) ...
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bioRxiv - Cancer Biology 2024Quote: Neuroblastoma BE(2)-C) cells were cultured in DME/F-12 1:1 (HyClone SH30023) supplemented with 10% Fetal Bovine Serum (Fisher Scientific FB12999102) and 1% Penicillin-Streptomycin (Lonza 17602E) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Biophysics 2023Quote: ... at a flow rate of 75 μL/min on a 15-cm C18 column maintained at 40°C (Acclaim Pepmap 1 mm I.D., 2 μm particle size, 100 Å pore size; Thermo Fisher Scientific). The LC method for DDA starts at 2% B and is held constant for 1 min ...
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bioRxiv - Biochemistry 2024Quote: ... Quantifoil R2/2 200-mesh copper grids with 2 nm carbon (EM sciences) at 4 °C and 100% humidity using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). The sample was blotted for 5s ...
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bioRxiv - Genomics 2023Quote: ... was reverse transcribed for 50 min at 50°C using 50 μM oligo dT and 2 μl of SuperScript III (Thermo Fisher Scientific) in a 20 μl reaction ...
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bioRxiv - Cell Biology 2023Quote: ... the feeder cells treated with 10 μg/ml mitomycin C (Nacalai tesque, Japan) for 2 hours were seeded at 2.0 × 104 cells/cm2 in α-MEM (Thermo Fisher Scientific) containing 10% FBS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed with 6 mL Accutase for 10 sec and incubated in 4 mL TrypLE select for 2 min/37°C before addition of 200 μL of Neuronal isolation enzyme (Thermo Scientific, 88285) and further incubating 1 min/37°C ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...