Labshake search
Citations for Thermo Fisher :
2151 - 2200 of 4028 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Cells infected with pLENTI-Luciferase-expressing virus (generous gift of Charlotte Kuperwasser, Tufts University) were selected with neomycin (G418, 500μg/mL, Gibco) for 2-3 weeks ...
-
bioRxiv - Molecular Biology 2023Quote: ... MED1-IDR containing plasmid was obtained from Addgene #145276 and inserted into pcDNATM6.2-C-EmGFP-DEST supplemented with the Simian Virus 40 NLS sequence by Gateway strategy (Invitrogen) using primers described in Suppl ...
-
bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
-
bioRxiv - Microbiology 2023Quote: RNA was extracted from the supernatant of MDCK cells infected with the PB2 TRAP V5 virus using Trizol (Invitrogen). RNA was reverse-transcribed with Superscript III (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... MRC-University of Glasgow Centre for Virus Research) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher, 10566016) supplemented with 10 % fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... A virus growth medium consisting of infection media supplemented with an additional 1 µg/mL trypsin-TPCK (ThermoFisher, USA) was added to the cells ...
-
bioRxiv - Bioengineering 2024Quote: The virus riboswitch (vRibo) RNAs were synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific Cat# K0441) from the DNA templates generated by PCR ...
-
bioRxiv - Microbiology 2024Quote: ... The first strand cDNA was obtained using oligo(dT) primers and Moloney murine leukaemia virus-reverse transcriptase (Life Technologies), using 100 ng of purified RNA ...
-
bioRxiv - Cell Biology 2024Quote: ... Virus was removed 16-20 hours following transduction with fresh neural media [Neurobasal medium (NBM; Life Technologies, #21103-049) supplemented with 1x N-2 supplement 1x B-27 supplement ...
-
bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
-
bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and virus pellet was resuspended in the same volume of RIPA buffer (Cat #89901, ThermoFisher, 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
-
bioRxiv - Cell Biology 2020Quote: ... T1249-Fc were expressed by transient transfection in 293-6E (CNRC) or Expi293 (ThermoFisher Scientific) cells and purified from transfected cell supernatants using a HiTrap MabSelect SuRe column (GE Healthcare ...
-
bioRxiv - Cell Biology 2019Quote: ... absorbance at 405 nm was measured in a MultiSkan FC microplate reader (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2021Quote: ... Both compensation controls and samples were washed twice with 200μL FACS buffer (2% FCS (Gibco) in PBS (Gibco)) ...
-
bioRxiv - Immunology 2020Quote: ... ECD region of mouse CLEC2 was cloned into mammalian expression vector pFuse-rIgG-Fc (Invitrogen). The construct was used to stable transfect HEK-293T cells with PEI method ...
-
bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
-
bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
-
bioRxiv - Neuroscience 2020Quote: ... 10% FCS and 100 units / ml of penicillin and 100 μg / ml of streptomycin (Gibco). U87MG ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were blocked with anti-mouse CD16/32 (Fc-block, Invitrogen, San Jose, CA, USA) for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11 ...
-
bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-rabbit IgG Fc secondary antibody (Invitrogen, Cat. No. 31463).
-
bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). MLV P30 protein within the pseudovirus capsid was detected using a rabbit anti-MLV-P30 polyclonal antibody (Origene ...
-
bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). Beta-actin was used as an internal control.
-
bioRxiv - Molecular Biology 2020Quote: ... Absorbance at 570 nm was measured using a spectrophotometer (Multiskan FC Microplate Photometer, Thermo Fisher). A standard curve was generated using standard glycogen solution in the reaction ...
-
bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
-
bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
-
bioRxiv - Microbiology 2021Quote: ... Cells were infected with the specified viruses and MOIs in DMEM without FCS (Life Technologies) for 1h at 37.0°C (Cts24 ...
-
bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) for 3 hours before fixation for 15 minutes (3.7% FA and 0.5% triton X-100) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and then blocked in PBS supplemented with 20% foetal calf serum (FCS, Thermo Fisher Scientific) and 20% normal goat serum (NGS ...
-
bioRxiv - Immunology 2022Quote: ... and were used after freezing in Fetal Calf Serum (FCS) (Invitrogen, Waltham, Massachusetts, United States) containing 10% DMSO (Sigma-Aldrich).
-
bioRxiv - Microbiology 2022Quote: ... Absorbance at 405 nm was read on a Multiskan FC plate reader (Thermo Scientific, USA).
-
bioRxiv - Biochemistry 2020Quote: ... SKOV3-cells were cultured in McCoy’s 5A Medium supplemented with 10% FCS (Thermo Fisher Scientific). HROC24-cells were cultured in DMEM/Ham’s F12 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... 1mM sodium pyruvate and 3.7 g/L NaHCO3 and supplemented with 15% FCS (Gibco, A3160801), 10 mM HEPES (Gibco ...
-
bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked with anti-mouse CD16/CD32 (20 µg/ml, clone 93; Invitrogen) for 20min ...
-
bioRxiv - Microbiology 2019Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (P/S; Thermo Fisher) in a humidified atmosphere at 37°C with 5% CO2 ...
-
bioRxiv - Physiology 2019Quote: ... Cells were kept in DMEM supplemented with 10% tetracycline-free Hyclone™ FCS (Fisher Scientific) and the above-mentioned antibiotics.
-
bioRxiv - Immunology 2020Quote: ... Cells were washed twice following staining and resuspended in FC buffer containing 7AAD (Life Technologies) or FVS700 (BD Bioscience ...
-
bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at 450 nm by a Multiskan™ FC Microplate Photometer (Fisher Scientific).
-
bioRxiv - Microbiology 2019Quote: ... supplemented with 4% FCS and 0.75% sodium bicarbonate containing 0.5% low melting agarose (Invitrogen, USA). The overlay was allowed to solidify at RT ...
-
bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS) and 2mM L-glutamine (all from Life Technologies). HEK293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) ...
-
bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% foetal calf serum (FCS) and 1mM Sodium Pyruvate (all from Life Technologies). DLD-1 and COLO205 cells were maintained in Roswell Park Memorial Institute (RPMI ...
-
bioRxiv - Biochemistry 2020Quote: ... The absorbance was measured with a Multiscan™ FC microplate photometer (Thermo Fisher Scientific, USA). Cell viability is presented as the percentage of control (untreated cells) ...
-
bioRxiv - Microbiology 2020Quote: ... Enzyme and Fc-portion was removed on HIS-Pur Ni-NTA resin (Thermo Fisher Scientific) and Protein G sepharose (GE Healthcare ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with 10% inactivated fetal calf serum (FCS) (Cat: a3520501 Thermo Fisher Scientific Inc, USA), an 1% antibiotic mixture (Cat ...
-
bioRxiv - Immunology 2020Quote: ... DMEM/HAM’s F12 (FAD) medium with low Ca2+ (50 µM) supplemented with 10% FCS (Gibco) treated with chelex 100 resin (Bio-Rad ...
-
bioRxiv - Immunology 2020Quote: ... When needed neutrophils were cultured in RPMI 1640 containing 10% fetal calf serum (FCS, Gibco), 2 mM L-glutamine ...
-
bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...