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Citations for Thermo Fisher :
1951 - 2000 of 4028 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... according to the manufacturer’s instructions using a Multiskan™ FC microplate photometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... Detection was performed with secondary goat anti-mouse IgG Fc HRP antibody (Invitrogen, A16084) and WesternBright Sirius chemiluminescent HRP conjugate (advansta ...
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bioRxiv - Neuroscience 2023Quote: ... single cell suspensions were incubated with Fc Block CD16/32 (Invitrogen; 14-0161-85). The cells were then incubated for 30 minutes with the surface antibodies and Live/Dead Ghost Dye Violet 510 (Tonbow Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, Gibco Cell Culture, 10270-106), 0.5% (v/v ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were incubated with Fc Block CD16/32 (Invitrogen, 14-0161-85). The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved at −150°C in 40% heat-inactivated fetal bovine serum (FCS, Gibco), 50% RPMI-1640 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with anti-CD16/CD32 (diluted 1:50, Thermo Fisher Scientific) and cells were stained with cell surface antigens ...
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bioRxiv - Cell Biology 2023Quote: ... After Fc receptor blockage using an antibody for CD16/32 (14-0161-85, Invitrogen), stainings were performed in FACS buffer (1 % BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10 % fetal calf serum (FCS, Gibco, ThermoFisher Scientific, Hanover Park, IL, USA). In order to induce the Tripz-construct cells were treated with a final concentration of 5 µg/ml doxycycline hyclate (DOX) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and Fc fusions were purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13 was purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions with the exception that all buffers were supplemented with 1tab/50mL of cOmplete Protease Inhibitor Cocktail and 10uM Pepstatin A ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FCS (Invitrogen, 10270-106, batch number 41F8126K), 2,000 units ml−1 LIF (Millipore ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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bioRxiv - Microbiology 2024Quote: ... 10% (v/v) heat inactivated fetal calf serum (FCS, Hyclone, Thermo Scientific, Rockford, IL), 100 U/mL penicillin ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human Fc chip was regenerated to baseline using IgG Elution Buffer (Thermo Scientific) and the process was repeated for all humAbs in the panel ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... All iPSCs were generated by Sendai virus reprogramming and clonal expansion using the CytoTune – iPSC 2.0 kit (ThermoFisher) to deliver the reprogramming factors ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2019Quote: ... Ka539 cells transfected with a virus expressing luciferase reporter were cultured in DMEM/high glucose and IMDM (Gibco) supplemented with 10% FBS (Natocor) ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated for 2 hours with the virus and then washed twice with Neurobasal-A medium (Gibco) containing 2% B-27 (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified via qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) on a QuantStudio 3 (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA extractions of 80 μL of virus stocks lyzed with 240 μL of TRIzol LS Reagent (Invitrogen) were performed in triplicate with the Direct-zol RNA MiniPrep kit (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... All the procedures follow the manufacturer’s instructions of the Applied Biosystems TaqMan Fast Virus one-step mastermix (ThermoFisher). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA was retrotranscribed to complementary DNA using moloney murine leukemia virus reverse transcriptase (Thermo Fisher) and random hexamer primers (Roche) ...
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bioRxiv - Pathology 2021Quote: ... Total RNA from virus supernatants was extracted using the PureLink™ Viral RNA/DNA Mini Kit (Thermo Fisher) according to the manufacturer’s protocol and then converted to cDNA by SuperScript® III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... we exchanged the medium in the filtered virus collections three times with Opti-MEM I (1X) + GlutaMAX (Gibco) using an Amicon Ultra-15 column (100 kDa cutoff) ...
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bioRxiv - Microbiology 2022Quote: ... quantitative polymerase chain reaction (qPCR) was performed using TaqMan Fast virus 1 Step PCR Master Mix (Applied Biosystems); a standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... Reverse-transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo (dT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative real-time PCR was performed using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), 2019-nCoV RUO Kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated to passage three on Vero E6 cells in Opti-MEM I (1X) + GlutaMAX (Gibco), supplemented with penicillin (10,000 IU/mL ...
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bioRxiv - Microbiology 2019Quote: FVG-R infections were performed by inoculating A549 cells with virus diluted in Opti-MEM I (Invitrogen, 31985070) supplemented with 0.2% FBS ...
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... Samples KAB_3 and KAB_7 were reprogrammed using Sendai virus (CytoTune™-iPS 2.0 Sendai Reprogramming Kit, ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: Concentrated virus particles were treated with RNase A before extraction of RNA using Trizol LS (Ambion, Life Technologies). The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2020Quote: Concentrated virus particles were treated with RNase A before extraction of RNA using Trizol LS (Ambion, Life Technologies). The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... Transmissible gastroenteritis virus [TGEV] and Swine deltacoronavirus [SDCoV] (VetMAX™ PEDV/TGEV/SDCoV, Thermo Fisher Scientific, MA, USA) and Senecavirus A [SVA] (EXOone Seneca Virus Valley ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo(dT ...
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bioRxiv - Synthetic Biology 2022Quote: ... undiluted SINV-containing supernatants were combined with the TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher, #4444434) and the appropriate primer-probe sets in technical triplicates ...
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bioRxiv - Microbiology 2022Quote: ... Premixes were prepared for each amplification reaction using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... C and S genes of the Hepatitis B virus (accession number: KY00323035) was ordered from GeneArt (ThermoFisher Scientific) and is available from Addgene (Addgene # 179155).
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bioRxiv - Neuroscience 2023Quote: ... C-Myc) introduced by Sendai virus according to the manufacturer’s instructions (Cytotune iPS 2.0 Sendai reprogramming kit, ThermoFisher) or by using an episomal method ...
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bioRxiv - Cell Biology 2023Quote: ... After a brief culture to expand the erythroblast population a Sendai virus based approach (CytoTune 2.0 Kit, Invitrogen) was used to express the Yamanaka factors without any genomic integration (27) ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Biochemistry 2023Quote: Bacmid preparation and virus production were performed according to the Bac-to-Bac baculovirus system manual (Gibco, Invitrogen). Spodoptera frugiperda Sf9 cells at a density of 3 × 106 cells/ml were co-infected with baculoviruses encoding receptor and Gi trimer at the ratio of 1:1 ...