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Citations for Thermo Fisher :
2001 - 2050 of 4028 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Epstein–Barr virus-transformed lymphoblasts were obtained from Coriell Cell Repositories and were grown in RPMI 1640 (Gibco) supplemented with 10% FBS and penicillin/streptomycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Immunology 2023Quote: ... Stock virus was sub-passaged through Madin-Darby canine kidney (MDCK) cells in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), harvested as tissue culture supernatant and viral titres determined by cytopathic effects on MDCK cells and expressed as the mean log10 tissue culture infective dose required to kill 50% of the cells (TCID50 ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA synthesis and PCR were performed using TaqMan™ Fast Virus 1-Step Multiplex Master Mix (#4331182; ThermoFisher). Probes were used to detect the mouse transcripts of Htr2a and Gapdh (Mm00555764_m1 and Mm99999915_g1 4448892 ...
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bioRxiv - Cell Biology 2023Quote: ... infected with Sendai virus containing Yamanaka factors from the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (ThermoFisher, A165167) according to manufacturer recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... 8 ml P4 virus was used to infect 800 ml cultures of Expi293F GnTI-Cells (Thermo Fisher Scientific) cells at 2-3 × 106 cells supplemented with 1% Fetal Bovine Serum (FBS) ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080], 5% FBS [Hyclone SH30070.03HI] and 1% Penn-Strep [Gibco 10378016]). Monoclonal antibody dilution plates were prepared similarly to serum dilution plates ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 10-fold serial dilutions of the virus for 1 h and overlaid with 0.55% methylcellulose or 1.0% cellulose in minimal essential medium (MEM) (Gibco) supplemented with 2% FBS (Corning ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Genomics 2024Quote: ... Virus was produced by transfecting HEK293T cells with the plasmid and the Lipofectamine 3000 kit (Thermo Fisher Scientific). U87MG were transduced with the supernatant containing the NY-ESO-1 virus ...
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bioRxiv - Microbiology 2024Quote: ... and CC81 (feline cell line transformed by mouse sarcoma virus ECACC 90031403) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-high glucose (GlutaMAX, Gibco) supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Virus production medium contained DMEM supplemented with L-glutamine and sodium pyruvate and 10% heat-inactivated FBS (Gibco). Viral supernatants collected roughly 48- and 72-hours post-transfection were sterile-filtered through a 0.45-micron filter and concentrated in 100kDa molecular weight cutoff filters.
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bioRxiv - Microbiology 2019Quote: ... alongside a synthetic CAP cDNA-HA sequence with C6,8A mutations (GeneArt strings, Life Technologies), was Gibson cloned (Gibson et al. ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Cell Biology 2019Quote: ... the following labeling reagents were used: rabbit anti-HA antibody (1:600; ThermoFisher, 715500), Alexa488- or Alexa647-conjugated goat anti-rabbit antibody (1:600 ...
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bioRxiv - Cell Biology 2019Quote: ... the following labeling reagents were used: rabbit anti-HA antibody (1:600; ThermoFisher, 715500), mouse anti-GFP antibody (1:600 ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used in blotting: rabbit anti-HA (1:1000; ThermoFisher, 715500), mouse anti-tubulin (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Staining for CS and HA GAGs with Mouse anti-CS-56 (Thermofisher, MA1-83055) and Biotinylated HABP (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was transferred to anti-HA magnetic beads (Thermo Scientific cat. no. PI88836) for the pulldown of intact lysosomes ...
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bioRxiv - Cell Biology 2021Quote: ... A KpnI to NotI digest was performed to clone RAD54-HA into pENTR1A (Invitrogen), followed by transfer into pLentiCMV/TO DEST#2 (Campeau et al ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were transfected with pLenti.ACE2-HA DNA by lipofection with lipofectamine 2000 (Invitrogen). After 2 days ...
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bioRxiv - Synthetic Biology 2021Quote: ... minicells were incubated with 1:200 dilution of anti-HA immunotag monoclonal antibody (Invitrogen) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... TcATAT-HA coding regions was then inserted into a pENTR3C vector (Gateway system Invitrogen) using the BamHI/XhoI restriction sites included in the oligonucleotides (underlined ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysates were then added to anti-HA antibody conjugated magnetic beads (Thermo Scientific) or RFP trap beads (Chromotek ...
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bioRxiv - Microbiology 2022Quote: ... or rabbit polyclonal antibody (pAb) anti-HA (diluted 1:1000) (Invitrogen, catalog no. PI715500). The Ty1 epitope was detected with mouse mAb BB2 (diluted 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal anti-HA (clone 2-2.2.14) DyLight 650 (1:1000; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... with the retroviral vector encoding MC4R-3×HA using Lipofectamine 3000 (Thermo fisher scientific), in accordance with the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... The ChIP was performed with Pierce Anti-HA Magnetic Beads (ThermoFisher Scientific, MA, USA) and was adapted from previous studies in human cell lines and Zymoseptoria tritici57,58 and our recently developed method for ChIP-Seq on Histone H3 (H3K4me2 ...
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bioRxiv - Cell Biology 2019Quote: ... the beads were eluted in 30µl 500µg/ml HA peptide (Thermo Fisher Scientific, #26184) at 37°C for 30mins ...
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bioRxiv - Cancer Biology 2020Quote: ... HA-tagged RON was cloned into the expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) by fusion PCR ...
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bioRxiv - Cell Biology 2021Quote: ... The Alexa Fluor 647-conjugated anti-HA antibody for dSTORM experiments was from Invitrogen (Renfrew ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixed lysate was incubated with pre-crosslinked HA Dynabeads protein G (Invitrogen 10004D) following the manufacturer’s directions ...
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bioRxiv - Immunology 2022Quote: ... The remaining 700µl were loaded on 75µl Pierce Anti-HA Magnetic Beads (Thermo Fisher) and pulled down at 4°C for 3 hours with constant rotation ...
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bioRxiv - Cell Biology 2022Quote: ... and NBPXa-CtermLoxP/HA constructs were first cloned into plasmid backbone (Geneart, Thermofisher Scientific), containing a multi-cloning site with SacII ...
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bioRxiv - Plant Biology 2023Quote: ... Conjugated anti-HA-HRP (Cat#12013819001, MilliporeSigma) and anti-FLAG-HRP (Thermal Fisher Scientific) antibodies were used to detect HA- and FLAG-tagged proteins ...
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bioRxiv - Plant Biology 2022Quote: ... BIK1 was detected in Western blots assays using HA-HRP antibody (Invitrogen, 26183-HRP)) and its phosphorylation was quantified by calculating the ratio between the intensity of the shifted upper band of phosphorylated BIK1 (pBIK1 ...
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bioRxiv - Cell Biology 2022Quote: ... They include the following antibodies and dilutions: mouse monoclonal anti-HA (Invitrogen 2-2.2.14) (1:250) ...
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bioRxiv - Developmental Biology 2023Quote: ... pH 7.6 (Rabbit anti-HA, Cell Signaling, 37245; rabbit anti-Podocin, Invitrogen, PA5-79757). Sections were incubated overnight at 4°C followed by 4×10 min washes in TBS/BSA-Ac to remove unbound antibody ...
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bioRxiv - Cell Biology 2023Quote: ... the purified protein was eluted using 2 µg/mL HA peptide (Thermo Scientific, 26184) in 50 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed and stained at 24 hpi with rabbit anti-HA (Thermo Scientific) followed by anti-rabbit Alexa Fluor 488 ...
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bioRxiv - Genetics 2023Quote: ... was used for anti-HA immunoprecipitation following manufacturer recommendations (#26180, Thermo Fisher Scientific, Gmbh). After immunoprecipitations ...
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bioRxiv - Biochemistry 2023Quote: ... or goat anti-rabbit secondary antibody (for HA; Thermo Fisher Scientific Cat# 31460, RRID:AB_228341) and ECL substrate (Thermo Fisher Scientific Cat# 32106).
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bioRxiv - Neuroscience 2024Quote: RNA has been extracted from myotubes differentiated for 7 days using Trizol (Life Technologies) followed by Chloroform/Isoamyl alcool (49:1 v/v) ...
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bioRxiv - Neuroscience 2023Quote: ... N2A cells were transfected with different HA tagged WDR47 variants using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysates were then added to anti-HA antibody-conjugated magnetic beads (Thermo Scientific) which had been equilibrated in NP-40 extraction buffer ...
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bioRxiv - Cell Biology 2024Quote: HA hydrogel embedded alveolar organoids were fixated in 4% paraformaldehyde (ThermoFisher Scientific Catalog #J61899) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were first incubated with rabbit anti-HA antibody (1:1000; ThermoFisher, #71-5500) overnight at 4°C and then with anti-rabbit 488 (1:200 ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...