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Citations for Thermo Fisher :
2401 - 2450 of 4235 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Pierce Trypsin/Lys-C Protease Mix and tandem mass tag (TMT)-labeling kit were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were purified via affinity pulldown of their StrepII tags using a StrepTrap HP column (Fisher Scientific) and eluted into Buffer A supplemented with 2.5mM desthiobiotin (Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tandem Mass Tag™ 6-plex (TMTsixplex™) reagents kits for isotopic labeling were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2022Quote: ... Desalted peptide samples from each sample were labeled with 10-plex tandem mass tags (TMT, ThermoFisher Scientific) with one of the TMT channels (131 ...
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bioRxiv - Microbiology 2022Quote: ... labelled with Tandem Mass Tag (TMT) eleven plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Physiology 2022Quote: ... 25 μg of peptides per sample were labeled with isobaric tandem mass tags (TMT10plex, Thermo Fisher Scientific) as described previously [48] ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The ATP2 proteins fused to the BAD tag were detected with the Avidin HRP-conjugate probe (Invitrogen), diluted to 1:20 000 in 5 % (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... and delivered into a modified pEG containing 3XFLAG and 9XMYC tags using the LR reaction kit (Invitrogen). The pEG destination vectors were transformed into Agrobacterium strain AGL0 ...
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bioRxiv - Molecular Biology 2020Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and pooled ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Systems Biology 2020Quote: ... 250 μg of peptide were labeled using TMT11Plex Mass Tag Labeling Kit (Thermo Scientific, Lot number UE283355) following the manufacturer’s instructions and they were combined as shown in Figure 1 ...
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bioRxiv - Microbiology 2020Quote: Proteins with C-terminal biotin tags were attached to streptavidin-coated dynabeads (Dynabead M-280 Streptavidin, Invitrogen) following standard procedures ...
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bioRxiv - Biochemistry 2021Quote: ... Desalted peptides were labeled using a Tandem Mass Tag 11-plex isobaric label reagent set (ThermoFisher #A34808) with the following setup ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2020Quote: ... the polyhistidine tag of 6xHis-TEV-dreC5a was removed by incubation with TEV protease (Thermo Fisher Scientific) and the protein was FITC-labelled at the N-terminus using the sortagging method (37) ...
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bioRxiv - Microbiology 2019Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled ...
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bioRxiv - Physiology 2020Quote: ... 25 μg of peptides were labeled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific) as described previously (34) ...
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bioRxiv - Biochemistry 2021Quote: ... as described in (62).The 6xHA with linker and mCitrine tags were cloned into pDONR-P2RP3 (Invitrogen) as described in de Saint Germain et al ...
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bioRxiv - Cell Biology 2021Quote: The LAP tag from pIC113 vector (Cheeseman and Desai, 2005) was subcloned into pcDNA5.0/FRT vector (Invitrogen) to make the pcDNA5.0/FRT-LAP-N vector ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat ...
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bioRxiv - Biochemistry 2021Quote: ... followed by a short linker FLAG-epitope tag and C-terminal Histidine6 was synthesised by GeneArt (Invitrogen). To generate a FLAG-free HuACE2 fusion ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... labelled with Tandem Mass Tag (TMT) 6-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting peptide mixtures were labelled with isobaric TMT-tags according to the manufacturer’s protocol (Thermo Scientific). Online LC-MS was performed using a Dionex UltiMate™ 3000 RSLCnano System coupled to a Q-Exactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (ThermoFisher Scientific, Inc) and the labelled samples pooled.
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bioRxiv - Immunology 2022Quote: ... and the MERS-CoV S protein with Avi-tag was conjugated to streptavidin-AF647 (Invitrogen cat.# S21374). After incubating the cells with Ab mixture for cell surface markers for 15 min in dark ...
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bioRxiv - Neuroscience 2022Quote: ... with 0.5 mg of Tandem Mass Tag Pro (TMTpro) reagent (16-plex kit, manufactures instructions Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cardiomyocytes were labeled with of DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, MA1-142-A488) at 1:500 dilution in 1% BSA ...
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bioRxiv - Systems Biology 2024Quote: ... The protocol was performed as in (Ref.16) with a V5-tag antibody (Thermo Fisher, R960-25) that was added to a final dilution of 1:100.
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bioRxiv - Microbiology 2024Quote: ... PCR amplification was subsequently performed using Platinum Tag DNA Polymerase High Fidelity (ThermoFisher Scientific, Cat. #: 11304-102). Amplicons of the same samples were pooled together before purification ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... Eluted peptides were vacuum dried and tandem mass tags labeling was performed (11-plex TMT, Thermo Scientific) by following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated overnight at 4°C with 100 ng of 6x His tag monoclonal antibodies (Invitrogen) in PBS and washed the next day ...
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bioRxiv - Biochemistry 2024Quote: ... Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher, P/N 90061). After labeling ...
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bioRxiv - Cancer Biology 2024Quote: ... Tandem Mass Tagging (TMT) labelling was performed using Tandem Mass Tag Reagents kit (Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Biochemistry 2023Quote: ... and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen). Supernatants were applied to Strep-Tactin XT resin (IBA ...
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bioRxiv - Cell Biology 2023Quote: ... CSS1 and CSS2 were synthesised together with a V5-tag using GeneArt DNA Synthesis (Thermo Fisher Scientific) and then cloned into pEU-Flexi ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... resuspended in 200 mM HEPES and incubated with 0.1 mg Tandem Mass Tag (TMTzeroTM, Thermo Fisher Scientific) label reagent for 2h at 26°C and 450 rpm ...
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bioRxiv - Cell Biology 2023Quote: A cDNA encoding p38α was subcloned into pcDNA6 with an N-terminal myc tag (Invitrogen, Carlsbad, CA), and a kinase-dead (D168A ...
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bioRxiv - Cell Biology 2023Quote: ... Tandem mass tag labeling (TMT) was performed using the TMT 10-plex reagent kit (ThermoFisher, Waltham, MA). Detailed methods of liquid chromatography-mass spectroscopy analysis is included in the Supplemental Material.
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bioRxiv - Cell Biology 2023Quote: ... Proteins of interest were labelled with antisera against the FLAG tag (Thermo Fisher Scientific, Waltham, MA, USA) and ATP5A (Abcam ...
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bioRxiv - Biochemistry 2023Quote: WDR5 and WDR5 N225A were cloned into the pEF-dest51 destination vector with a V5 tag (Invitrogen) resulting in the expression clones pEF-dest51-WDR5 and pEF-dest51-WDR5-N225A ...
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bioRxiv - Molecular Biology 2023Quote: ... HDR templates containing the FKPB12F36V tag were synthesised (see supplementary table 1) and cloned by GeneArt (Invitrogen) into double-stranded plasmid vectors (pMX).