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Citations for Thermo Fisher :
2351 - 2400 of 4235 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... MED1-IDR containing plasmid was obtained from Addgene #145276 and inserted into pcDNATM6.2-C-EmGFP-DEST supplemented with the Simian Virus 40 NLS sequence by Gateway strategy (Invitrogen) using primers described in Suppl ...
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bioRxiv - Microbiology 2023Quote: ... MRC-University of Glasgow Centre for Virus Research) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher, 10566016) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: The virus riboswitch (vRibo) RNAs were synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific Cat# K0441) from the DNA templates generated by PCR ...
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bioRxiv - Microbiology 2024Quote: ... The first strand cDNA was obtained using oligo(dT) primers and Moloney murine leukaemia virus-reverse transcriptase (Life Technologies), using 100 ng of purified RNA ...
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bioRxiv - Physiology 2024Quote: ... and infected with sendai virus (SeV) carried polycistronic Klf4-Oct3/4-Sox2 and Klf4 vectors (ThermoFisher, Waltham, MA, #A16517), here referred as OSK-SeV ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we extracted virus from the pooled samples from each assay grid using a sterile pellet pestle (Fisher Scientific, U.S.A.) to manually burst larvae and release viral occlusion bodies ...
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bioRxiv - Cell Biology 2024Quote: ... the virus titer was quantified using quantitative polymerase chain reaction (qPCR) with SYBR Green Master Mix (Thermo Scientific, 4309155) and primers targeting ITRs (Supplementary Table 1) ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted from the supernatant of MDCK cells infected with the PB2 TRAP V5 virus using Trizol (Invitrogen). RNA was reverse-transcribed with Superscript III (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... at a 6:1:2 ratio for each virus using the Bac-to-Bac baculovirus expression system (Thermo Fisher). Cell cultures were grown in ESF 921 medium (Expression Systems ...
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bioRxiv - Immunology 2024Quote: ... RNA was then reverse transcribed from 200 ng total RNA with Moloney murine leukemia virus reverse transcriptase (Invitrogen #28025013) using oligo (dT)18 (Thermo Scientific #SO131 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were probed with mouse anti-HA (12CA5) and mouse anti-Pgk1 (22C5D8, Molecular Probes), followed by probing with mouse anti-Pgk1 (22C5D8 ...
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bioRxiv - Molecular Biology 2021Quote: ... had an RPL3-HA gene that had been inserted using the Flp-In (Thermo Fisher) system ...
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bioRxiv - Cell Biology 2022Quote: ... that has been biotin-labeled with EZ-Link™ Sulfo-NHS-LC-LC-Biotin (ThermoFisher) was mixed with 50 μg/mL competitor antibody before adding into the coated plates ...
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bioRxiv - Microbiology 2019Quote: ... This DNA fragment and a synthetic CAP cDNA-BamHI-HA sequence (GeneArt strings, Life Technologies), were Gibson cloned (Gibson et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... or 100 ng pBJ5-SERINC5-HA or empty pBJ5 plasmid and Opti-MEM media (Gibco). The transfection mix was then added to Lipofectamine 3000 diluted in Opti-MEM and incubated at room temperature for 15 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The THAP9-H cell lysate was incubated with 5ug of HA antibody (Invitrogen, 71-5500) at 4°C for 14-16h on a rotating tumbler followed by incubation with protein G sepharose beads (GE ...
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bioRxiv - Plant Biology 2021Quote: ... to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher) to pull down AGO2-HA ...
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bioRxiv - Neuroscience 2020Quote: ... 1X PBS) before the addition of antibodies (1:400 mouse anti-HA (Thermo Fisher Scientific) or Streptavidin-HRP (Genscript ...
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bioRxiv - Microbiology 2020Quote: ... that has been pre-labelled using Alexa Fluor™ 647 Protein Labeling Kit (A20173, Invitrogen) for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2019Quote: Neuraminidase (NA) and haemagglutinin (HA) genes of the following strains were synthesised by GeneArt (Invitrogen) and cloned into a pHW2000 vector [68] ...
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bioRxiv - Cell Biology 2019Quote: ... the extracts were incubated with 30µl mouse anti-HA magnetic beads (Thermo Fisher Scientific, #88836) at 4°C for 3 hours ...
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bioRxiv - Immunology 2020Quote: ... HA ectodomain trimer constructs were expressed by transient transfection using the Expi293 Expression System (ThermoFisher), and soluble HA trimers were purified from transfected cell supernatants by standard Ni-NTA chromatography using a prepacked HisTrap™ HP column (GE Healthcare ...
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bioRxiv - Physiology 2020Quote: ... the organ has been perfused with 50 mg of a dextran (Dextran fluorescein; Molecular Probes), solubilized in 2 mL of Tyrode Solution ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5% Triton X-100) and bound to 40 μl Pierce anti-HA magnetic beads (ThermoFisher) for 4 h rotating at 4°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each C-terminally HA tagged GC-C variant was synthesized via gene synthesis (Life Technologies). GC-C was then cloned into the lentiviral transfer vector pUltra (Addgene #24129 ...
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bioRxiv - Microbiology 2021Quote: ... the supernatant was applied to 100 µL of Pierce α-HA magnetic beads (Thermo Scientific) in a 50 ml tube and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated mutant Cal09-HA (1.5μg) was complexed with streptavidin conjugated to APC (8μL; Thermofisher #S868) or PE (8μL ...
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bioRxiv - Biochemistry 2022Quote: ... Agarose-conjugated antibodies used for immunoprecipitations included Anti-HA agarose (Thermo-Fisher Scientific, Waltham, MA), and Anti-FlagM2 agarose (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were extracted and purified using Pierce Anti-HA magnetic beads (ThermoFisher Catalog number 88836) for 3 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... lysates were incubated with 40 μl of mouse anti-HA agarose beads (Thermo Fisher 26182) at 4°C on a turning wheel for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... at least 400ug of lysate and 25uL of Dynabeads conjugated with either HA-antibody (ThermoFisher) or MYC-antibody (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies used were mouse anti-Ty (76) and rabbit anti-HA (71-5500, Invitrogen) or rabbit anti-TgACT1(77) ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was cut into slices and probed with mouse anti-HA (Invitrogen 2-2.2.14) (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: Primary antibodies used in the IFAs included rabbit-anti-HA (SG77; ThermoFisher Scientific; 1:100), mouse-anti-RAP1 (2.29 ...
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bioRxiv - Cell Biology 2024Quote: ... Clarified lysates were incubated with 25 µl of anti-HA magnetic beads (Thermo Scientific, 88837) overnight at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The remainder was incubated with equilibrated anti-HA coupled Protein G magnetic beads (12CA5, Thermofisher) for 2.5 hours at 4°C on a rotator ...
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bioRxiv - Genetics 2023Quote: ... 25 µl (1/10th homogenate volume) of Pierce Anti-HA Magnetic Beads (Thermo Scientific, 88836) were washed with 175 µl 1X TBS-T (Pierce 20X TBS Tween 20 Buffer ...
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bioRxiv - Molecular Biology 2023Quote: Frog oocyte or embryo lysate was incubated with anti-HA magnetic beads (Thermo Fisher, 88837) using 20 µl slurry per 500 µl lysate ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were incubated with anti-c-myc or anti-HA magnetic beads (Thermo Scientific) for 2-4 hours and washed with the same buffer without phosphatase inhibitors ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-afadin (clone D1Y3Z) or Alexa Fluor 488 anti-DYKDDDDK (flag) tag monoclonal antibody (L5, Life Technologies). The plasma membrane was stained with CF594 dye conjugated wheat germ agglutinin (Biotium) ...
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bioRxiv - Cell Biology 2019Quote: ... and pENTR-Megator constructs with pHWG (blasticidinR; C-terminal EGFP tag) using Gateway LR Clonase II (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were immunoprecipitated by incubating with an antibody to the c-Myc tag (Invitrogen 13-2500) on BBS2 as described above and immunoblotted with the c-Myc antibody for BBS2 and with the anti-Flag antibody (Sigma F3165 ...
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bioRxiv - Neuroscience 2020Quote: ... Tandem Mass Tags (TMT 10-plex) kits and microBCA protein assay kit were purchased from Thermo Fisher Scientific (Rockford ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained with anti-GFP antibody (5 μg/ml, Thermo Fisher, RFP Tag Monoclonal Antibody RF5R) and whole node confocal scans were used to quantify percent metastatic area of total node via image thresholding in ImageJ ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Biophysics 2020Quote: ... the kdpFABC operon with the 8x histidine tag was cloned into the pBAD vector (Invitrogen, Carlsbad CA) and introduced into E ...