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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... T-cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of PBMC-derived CECs (ratio 1:2 ...
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bioRxiv - Bioengineering 2019Quote: ... 10x of mouse (for NIH 3T3 cells) or human (for HCT116 cells) Cot-1 DNA (Thermo Fisher Scientific Cat ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor conjugated secondary antibodies used: goat anti-human Alexa-488 (1:1000, Cat. # A11013, Life Technologies), donkey anti-mouse Alexa-594 (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Human PDCD1 or PDL1 cell surface protein staining was analyzed using anti-PD-1 (ThermoFisher clone MIH4) or anti-PD-L1 (ThermoFisher clone MIH1 ...
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bioRxiv - Microbiology 2021Quote: ... 50 µL of a 1:100 dilution of mouse anti-human IgM phycoerythrin conjugate (Invitrogen, MA1-10381) or goat anti-human IgG phycoerythrin conjugate (Sigma ...
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bioRxiv - Immunology 2020Quote: ... and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482, 1:10,000).
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bioRxiv - Cell Biology 2019Quote: ... The secondary anti-human TRITC antibody (Thermo Fisher; 1:200 in 0.01% acBSA in PBS, pH 7.2) was used in this assay ...
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bioRxiv - Cell Biology 2020Quote: ... Released IL-1β was quantified using the Human IL-1 beta ELISA Ready-Set-Go! Kit (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 105 hCD3+ T cells were mixed with human T-Activator CD3/CD28 Dynabeads (Thermofisher, ratio 3:1) or PHA (1μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... 1×106 cells of human fibroblast cells at early passages were trypsinized (0.25% Trypsin/0.5mM EDTA, Gibco) and electroporated with indicated episomal plasmids using Amaxa Basic Nucleofector Kit for primary mammalian fibroblasts ...
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bioRxiv - Microbiology 2021Quote: ... resting or stimulated for 1 hr with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell) ...
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bioRxiv - Cell Biology 2022Quote: Human monocytic cells THP-1 (ATCC) were cultured in RPMI-1640 media supplemented with 10% FBS (Gibco), 50 U/mL penicillin ...
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bioRxiv - Cell Biology 2022Quote: Human monocytic cells THP-1 (ATCC) were cultured in RPMI-1640 media supplemented with 10% FBS (Gibco), 50 U/mL penicillin ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... Wells were washed 3 times and incubated with 1 μg/ml biotinylated anti-human IgE (A18803, Invitrogen) in 1% skim milk for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were counted and plated according to Table 1 in ECM (human endothelial Serum Free Media (Invitrogen) with 1% platelet-poor plasma-derived bovine serum (Alfa Aesar J64483) ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of conditioned media were analyzed by ELISA (IL-1 beta Human ELISA Kit, Thermofisher KHC0011), using manufacturer protocol ...
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bioRxiv - Immunology 2020Quote: ... flow cytometric analysis was performed using rabbit anti-human ANXA1 (Thermo Fisher Scienific, Ulm, Germany, 1:100) and Alexa Fluor 647-conjugated goat anti-rabbit IgG H+L (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of a secondary Alexa Fluor 647 goat-anti human antibody (1:500) (Life Technologies A21455) was used ...
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bioRxiv - Microbiology 2022Quote: ... The remainder was stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA (Fraction V ...
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bioRxiv - Cell Biology 2022Quote: The assay was performed using the 1-Step Human Coupled IVT Kit (# 88882, Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions using ‘turbo-type’ green fluorescent protein (tGFP ...
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bioRxiv - Genetics 2022Quote: ... A mix of 6 ul of nick translated RNA probe with 12ul of Human Cot-1 (ThermoFisher) and 2ul of salmon testes (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with an anti-human Alexa Fluor 488 conjugate (1:200, A-10631, Thermo Fisher). For comparing tumor cell growth in different groups ...
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bioRxiv - Immunology 2023Quote: ... Anti-mouse ZAKa antibody (proteintech, #14945-1-AP) and anti-human ZAKa antibody were from Thermo Fisher Scientific (A301-993A) ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with primary antibodies: rat anti-human Gal3 (Invitrogen #14-5301-82, 1:50 dilution) and mouse anti-human CD68 (Dako #M0814 ...
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated for 1 h with goat anti-human IgG Alexa Fluor 647 (Invitrogen) and goat anti-mouse IgG Alexa Fluor 488 (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 human monocytes (ATCC) were maintained in Roswell Park Memorial Institute 1640 Medium (RPMI 1640, Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... 1:2000 dilution of secondary Alexa FLUOR-647 GOAT-Anti human IgG [H+L] (Invitrogen; #A-21445) was added to the cells for 30 minutes followed by 3 washes with FACS Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary staining was done using a goat anti-Human Alexa Fluor (AF) 594 antibody (1:1000, Invitrogen), for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...
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bioRxiv - Genomics 2019Quote: ... Hi-C DNA bound to capture RNA was isolated using a DynaMag-2 magnet (Life Technologies). Washes (15 min in 500 μl wash buffer I at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Completed reactions were ethanol precipitated and re-suspended in deionized formamide (Hi-Di formamide, Thermo Fisher) with the G-ladders ...
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Biochemistry 2020Quote: ... optiMEM (1X) reduced serum medium and heat-inactivated fetal bovine serum (HI-FBS) were from Gibco chemicals ...
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bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
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bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... All cloning was performed using a pcDNA 3.1-V5/His TOPO-TA cloning kit (Invitrogen K480001) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... This mix was added to 50 µl pre-washed His-Pur magnetic Nickel beads (Thermofisher Scientific) and incubated for 1 h at 4 °C on a rotor ...
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bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.