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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Recombinant constructs were co-expressed with BirA in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media supplemented with 50 mM biotin ...
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bioRxiv - Pathology 2022Quote: ... and incubated with PierceTM Recombinant Protein L Biotinylated (60µL per kidney, ThermoFisher #29997) in a tube rotator (25min. ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant 3CLpros were expressed in Escherichia coli BL21 cells (Invitrogen, Carlsbad, CA) grown in Luria Bertani broth induced with 1mM isopropyl ß-D-thiogalactopyranoside ...
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bioRxiv - Developmental Biology 2022Quote: ... The recombinant plasmids were introduced into HEK293-EBNA cells (Invitrogen, Carlsbad, CA, USA) using FuGENE 6 transfection reagents (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant zebrafish Nid2a and Hmcn1 fragments were generated in HEK293-EBNA cells (Invitrogen) and purified as described above for the mouse proteins and used to immunize rabbits or guinea pigs (Pineda Antikörper Service ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant TTR was detoxified using a high-capacity endotoxin removal resin (Thermo Scientific) and quantified using the Lowry based DC Protein Assay (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2020Quote: ... the recombinant plasmids were linearized with PacI before transfection with Lipofectamine 2000 (ThermoFisher) into the adenovirus packaging cell lines HEK293 cells ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 20 U/ml RNase-free Recombinant DNase I (Invitrogen, Cat # AM2235) and incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant BACs were purified using PureLink™ HiPure Plasmid Midiprep Kit (ThermoFisher Scientific) and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant N protein was purified using Ni2+ resin (Thermo Fisher Scientific, USA). An indirect ELISA assay was conducted with the recombinant N protein as the coating antigen (3 ng/μl ...
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bioRxiv - Cancer Biology 2022Quote: ... and purified recombinant Cas9 proteins (250 ng/ml: Thermo Fisher Scientific, MA, USA). The gRNA sequences are as follows:
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bioRxiv - Microbiology 2019Quote: ... cells were activated by adding 50 ng/mL recombinant mouse IFN gamma (Invitrogen). On day 7 ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... The samples were mixed with recombinant protein G-Sepharose 4B beads (Thermo Fisher) and 1 µl anti-Flag antibodies (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... The constructs were used for generating recombinant baculovirus in Sf21 insect cells (Invitrogen). RBA1 and RBA1 mutant proteins were expressed in Sf21 insect cells with recombinant baculovirus infection at 28 °C for 48 hr ...
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bioRxiv - Immunology 2021Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 μL (20 U) of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Life Technologies, USA) was added to each aliquot and inverted to mix ...
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bioRxiv - Neuroscience 2020Quote: ... to enhance viral expression (primary antibody: GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the nucleotide sequence was inserted by means of a BP recombinant reaction (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Recombinant lentiviruses were produced by transient transfection in HEK293FT cells (Invitrogen, CA, USA), as described earlier 52 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were generated using the Bac-to-Bac Baculovirus Expression system (Invitrogen). Sf9 cells were cultured in Sf-900 III SFM medium (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant viruses were generated using the Bac-to-Bac Baculovirus expression system (Invitrogen). The proteins were expressed by infecting 50 ml of Sf9 insect cells at density of 1.0 x 106 ml−1 with the above-described viruses ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were generated using the Bac-to-Bac baculovirus expression system (Invitrogen) and the proteins were expressed in Sf9 insect cells infected at a density of 1.2 x 106 ml−1 with P3 baculovirus stocks in a 1:1 (v/v ...
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bioRxiv - Cell Biology 2021Quote: This recombinant S protein was overexpressed using the HEK 293F mammalian cells (Invitrogen) at 37°C under 5% CO2 in a Multitron-Pro shaker (Infors ...
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bioRxiv - Biochemistry 2021Quote: Recombinant proteins were labeled with Alexa Fluor™ 647 NHS ester (Thermo Fisher) as described previously.20 In brief ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM Pierce™ recombinant protein A (#21184, Thermo Fisher Scientific, Waltham, USA) 50 nM bovine serum albumin (#A4612 ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was removed with recombinant DNase (TURBO DNA-free™ Kit, Ambion). Concentration and purity of RNA were both evaluated with NanoDrop spectrophotometer ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells for recombinant antigen and monoclonal antibody production (Thermo Fisher Scientific, A1452) were maintained at 37°C in 8% CO2 in Expi293F expression medium (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... and ligated to produce recombinant constructs using T4 DNA ligase (Thermo Fisher Scientific). Ligation reactions contained 50 ng of vector ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection ...
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bioRxiv - Neuroscience 2022Quote: ... recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock ...
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bioRxiv - Neuroscience 2024Quote: For recombinant lentivirus production - HEK293FT cell line (cat# R70007, ThermoFisher Scientific, Auckland, NZ), HT1080 cell line (cat# CCL-121 ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Genomics 2022Quote: ... Recombinant plasmids were transfected into cell lines using Lipofectamine 2000 (Invitrogen; cat.no: 11668019). Vector backbone and unmethylated constructs were used as internal controls for comparison ...
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bioRxiv - Microbiology 2023Quote: ... Secondary horseradish peroxidase (HRP)-conjugated recombinant A/G protein (Invitrogen, lot number XH346151) diluted 1:20,000 was used for detection and visualized with KPL TMB two-component peroxidase substrate kit (SeraCare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant T-REx-HEK-CFTR cells were generated by lipofectamine transfection (Life Technologies) of plasmids containing mutated versions of CFTR (RefSeq NM_000492) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were quantified with Pierce™ BCA Protein Assay Kit (Life Technologies) and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant chimeric antibodies were produced using the Expi293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Physiology 2023Quote: ... Recombinant TEVp was purified as previously described1 or was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and incubated with recombinant enzymes TrypLE (Thermo Fisher) for 10 minutes at 37°C for single cell dissociation ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50 ng of recombinant Gag/well (35) in 1xPBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 ng/ml recombinant noggin (Thermo Fisher Scientific, cat# 120-10C-250UG). The 3dGRO™ human ISO72 colorectal cancer tumoroid line was purchased from Millipore Sigma (Burlington ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...
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bioRxiv - Genomics 2019Quote: ... Hi-C DNA bound to capture RNA was isolated using a DynaMag-2 magnet (Life Technologies). Washes (15 min in 500 μl wash buffer I at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Completed reactions were ethanol precipitated and re-suspended in deionized formamide (Hi-Di formamide, Thermo Fisher) with the G-ladders ...