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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: THP-1 cells (1.0 × 106) were transfected with human siRNA (10 nM) using Lipofectamine 3000 (Invitrogen, Waltham, MA). All siRNAs were ON-TARGETplus SMARTpool (Dharmacon ...
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bioRxiv - Neuroscience 2021Quote: ... human DRGs immediately were cut into 1-2 mm pieces and stored in RNA-later (ThermoFisher, Cat# AM7021). For ISH ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibody was incubated overnight at 4°C [human Pax6 polyclonal antibody (1:100, Thermo Fisher Scientific)] ...
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bioRxiv - Immunology 2019Quote: Human monocyte THP-1 cells from ATTC were maintained in Roswell Park Memorial Institute (RPMI-1640) medium (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Evolutionary Biology 2020Quote: Cells in 6-well plates were transfected with 1 ug human or cow CD44-promoter-pNL2.1 or empty vector pNL2.1 using Lipofectamine 3000 (Invitrogen) as manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 1 mM EDTA.) with the following anti human antibodies: anti CD3-APC-eFluor 780 (Invitrogen, 47-0037-41), anti CD14-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Positive proliferation control wells were set up by plating 1 Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) for every 200 T-cells ...
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bioRxiv - Immunology 2021Quote: ... The human multiple myeloma cell line XG-1 was cultured in Iscove modified Dulbecco medium (Invitrogen Life Technologies) containing 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... The human multiple myeloma cell line XG-1 was cultured in Iscove modified Dulbecco medium (Invitrogen Life Technologies) containing 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
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bioRxiv - Cell Biology 2022Quote: Human acute monocytic leukemia THP-1 (ATCC, TIB-202™) cells were cultured in RPMI-1640 medium (Gibco) supplemented with heat-inactivated 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human NRP-1 Ab (AD5-17F6; Milteny Biotec) pre-labeled with Alexa Fluor 647 (Molecular Probes, A20186), and DAPI ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated with mouse anti-human E-cad ectodomain mAb (1:5000) (HECD-131700, Invitrogen, USA) for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... Human monocyte cell line THP-1 cells were cultured in RPMI 1640 medium with added 10% FBS (Gibco) and 1% penicillin-streptomycin (Hyclone) ...
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bioRxiv - Bioengineering 2023Quote: Human Osteosarcoma cell line SJSA-1 (formerly OsA-CL) was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human Osteosarcoma cell line SJSA-1 (formerly OsA-CL) was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Microbiology 2023Quote: ... IL-1β secretion from PMA-THP1 was detected using IL-1 beta Human ELISA kit (#KHC0011, ThermoFisher Scientific) and IL-1 beta Human ELISA kit ...
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bioRxiv - Immunology 2023Quote: ... the ELISA plates were incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (1:5000, Invitrogen) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Human RPE-1 cells were cultured in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F- 12 (DMEM/F12) (GIBCO), supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with Alexa Fluor 647-conjugated goat anti-human antibody (Invitrogen; cat# A-21445, 1/500). The control VHH Fc (R3VQFc ...
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bioRxiv - Cell Biology 2024Quote: ... The human monocytic THP-1 cell line (from ATCC) was cultured in RPMI1640 medium (Thermo Fisher Scientific Inc.) supplemented with 10% FBS and stimulated with OxLDL1 and OxLDL2 at a concentration of 20 µg/mL.
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bioRxiv - Neuroscience 2024Quote: ... supplemented with with 5% human platelet lysate (hPL, Stemmatters, Portugal) and 1% Penicillin-Streptomycin (P/S, Invitrogen USA). ASCs were maintained at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 48h at 4°C in the same solution with mouse anti-NeuN (1:2000; Chemicon; MAB377, Temecula, CA, USA) and anti-human PHF-Tau antibody clone AT8 (1:2000, MN1020, Thermo Scientific, Waltham, MA, USA); for the sake of clarity ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed 4X in PBST and subsequently incubated with secondary antibodies (goat anti-rabbit IgG 488, Invitrogen, Carlsbad, CA; #A-11034, 1:4000; & goat anti-human 647, Invitrogen #A-21445, 1:4000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
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bioRxiv - Cell Biology 2020Quote: ... The pDONR201 clones were transferred into GFP or SFB tagged DEST vectors using the Gateway LR cloning system (Invitrogen). KDM5AΔPID deletion mutant was generated by PCR in pDONR201 vector following standard cloning method and then subcloned into Gateway destination vector derived from pcDNA5/FRT/TO plasmids containing the NLS and SFB- or GFP-tagged in N-terminal ...
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bioRxiv - Cell Biology 2020Quote: ... GFP or luciferase tagged versions of the proteins were generated using the Gateway recombination cloning procedure (Invitrogen, Carlsbad, CA) and custom-made vectors.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells grown on 100-mm dishes were cotransfected with Flag-tagged ASK1_KR and PKA or empty vector using Lipofectamine 2000 (Invitrogen). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (Tf) and Epidermal Growth Factor (EGF) tagged with Alexa Fluor 488 was obtained from Invitrogen (T13342 and E13345). Dako fluorescent mounting medium from Agilent (S3023 ...
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bioRxiv - Genomics 2020Quote: ... 20.25 μg 3×Flag-tagged dCas9 plasmid and 20.25 μg sgRNA plasmid were transfected into cells using Lipofectamine 3000 (Invitrogen). Two biological replicates were performed for each condition ...
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bioRxiv - Cancer Biology 2021Quote: Control MCF-10A cells or cells expressing HA-tagged PEAK3 were cultured in -EGF medium (DMEM/F-12 (Gibco) supplemented with 0.5◻μg/ml hydrocortisone (Sigma) ...
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bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
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bioRxiv - Biophysics 2021Quote: ... and the tagged SΔTM was purified by affinity chromatography using Ni-NTA agarose beads (Invitrogen™, Waltham, MA, USA) and size exclusion chromatography as described above.
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bioRxiv - Microbiology 2021Quote: ... bacteria were tagged by Gfp or stained by cell membrane stain FM4-64 (10 µM final concentration, Molecular Probes) for 2 mins in the dark (without flow) ...
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bioRxiv - Cell Biology 2021Quote: ... Stable cell lines expressing BioID tagged genes of interest were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5/FRT/TO construct and Flipase expressing pOG44 plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified A-C/EBP (30 - 40 μg) was tagged with Alexa Fluor 488 according to manufacturer’s protocol (Thermo fisher). Time lag expression of A-C/EBP protein was observed using EGFP tagged A-C/EBP plasmid (pCMV-A-C/EBP-EGFP ...
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bioRxiv - Immunology 2019Quote: ... The labeled cells were counter-stained with a fluorophore-tagged secondary goat anti-rabbit IgG (H+L) antibody (Invitrogen), before acquisition on a LSR Fortessa (BD Biosciences) ...
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bioRxiv - Biophysics 2020Quote: ... The C-terminally SBP-tagged DISP1-A or DISP1-A-NNN was captured using Streptavidin-agarose affinity resin (ThermoFisher). The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff ...
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... captured dsRNA was detected by incubating with sulfo-tagged rat anti-mouse IgM antibody (Thermo Fisher Scientific, Waltham, MA) for 1h before washing and adding MSD GOLD Read Buffer A (Meso Scale ...
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...