Labshake search
Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The RAW 264.7 macrophage cell line (referred to herein as RAW cells) (ATCC TIB-71) and CD300lf-expressing Huh-7 cells were maintained in DMEM-10 medium (Gibco DMEM medium #11995-065 with 4.5 g/L D-Glucose and 110 mg/L Sodium Pyruvate ...
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bioRxiv - Neuroscience 2023Quote: ... or dominant negative (DN) CaMKII were transfected into COS-7 cells using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific). COS-7 cells were lysed in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5 ng/mL IL-7 (Miltenyi, USA) and 12.5 ng/mL IL-15 (Miltenyi, USA) or RPMI 1640 (Gibco, USA) medium supplemented with 100 U/mL IL-2 (Sci-Store ...
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bioRxiv - Neuroscience 2023Quote: ... Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco; UK) on ice ...
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bioRxiv - Microbiology 2023Quote: ... Five to 10 μg of total RNA were separated on a 7% (v/v) polyacrylamide gel (7.3 M urea, 1× TBE) and compared to RiboRuler LowRange RNA ladder (ThermoFisher Scientific). RNA from the gel was then blotted on a nylon membrane (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... and the Applied Biosystems® ViiATM 7 Real-Time PCR System with 384-well Block (Thermo Fisher Scientific; Cat #4453536). We used the comparative Ct (ýýCt ...
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bioRxiv - Biochemistry 2023Quote: ... buffer was exchanged to 200 mM ammonium acetate (pH 4.5) with a Zeba™ 7 K MWCO desalting column (ThermoFisher). After buffer exchange ...
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bioRxiv - Cell Biology 2023Quote: ... Cyclin B14E7E cells were selected for 72 h with Nutlin-3A 5 µM and for 7 days with Geneticin (Gibco) 0.4 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... following manufacturer’s protocols and gene expression quantified via Applied Biosystems Quant Studio 6/7 Real-Time PCR System (Applied Biosystems), for TaqMan (Thermo Fisher Scientific ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... and on a and on a ViiA 7 Real-Time PCR System using PowerTrack SYBR Green Master Mix (Applied Biosystems) for SV-EnhΔ samples ...
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bioRxiv - Immunology 2023Quote: ... and washed with HBSS and resuspended in appropriate volume of HBSS pH 7.4 supplemented with 5% FBS before staining with 7-aminoactinomycin D (Life Technologies) to exclude dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... was peeled off from the underlying tissue using a wet sterile cotton swab (7) and placed in Opti-MEM medium (Invitrogen) containing Pen-Strep ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Neuroscience 2023Quote: ... The relative levels of mRNA expression were examined utilizing real-time PCR (Applied Biosystems ViiA 7 Real-Time PCR System) with SYBR® GreenER™ reagent (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonads were then dissected in 7% MgCl2·H2O on ice and stored in RNAlater (Thermo Fisher Scientific Inc., Waltham, USA), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Animals were kept individually in 6-well plates with 7 mL of 0.22 µm FASW and final concentration of 1,000 U/mL penicillin and streptomycin (Fisher Scientific, 15140122). Feeding began 3 days post amputation to allow for the gut to reform ...
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bioRxiv - Molecular Biology 2024Quote: The fluorescence-microscopy based analysis of proliferation described above for the screen was used to validate the effect of the 7 selected miRNA mimics (Dharmacon, ThermoFisher) on HPASMCs (n=4) ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... RT-qPCR assays were carried out in accordance with the manufacturer’s instructions on the QuantStudioTM 7 Flex RT PCR System (Applied Biosystems), utilizing the indicated amplification cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... or 96-well flat-bottom plates at a concentration of 7 × 105 – 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco). For both workflows ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 58°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). For the telomere primers ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 56°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). Each sample was run in duplicate ...
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bioRxiv - Neuroscience 2024Quote: ... was performed using the Applied Biosystems QuantStudio 7 Flex Real-Time PCR system and analysed with the QuantStudio Real-Time PCR Software (v1.7 Thermo Fisher). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... Liposomes at 5-7 mM were applied to the carbon side of a grid held in a Vitrobot Mark IV (ThermoFisher) chamber kept at 4°C and 100% relative humidity ...
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bioRxiv - Cell Biology 2024Quote: ... 60L°C/60Lseconds), and melt curve (95L°C/15Lseconds, 60L°C/60Lseconds 95L°C/15Lseconds) on a QuantStudio 7 Flex (ThermoFisher). Messenger RNA (mRNA ...
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bioRxiv - Immunology 2024Quote: ... BHK-21 cells (7×105/well) were transfected in tissue culture-treated 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) with the LCMV Arm plasmids pCAGGS-NP (0.8 µg) ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s directions on a QuantStudio 7 Flex Real-Time PCR System (Cat. No. 4485701, Applied Biosystems, Waltham, MA). Amplification data and melt curves were analyzed using the QuantStudio Real-Time PCR software v1.7.1 ...
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bioRxiv - Immunology 2024Quote: ... The protein was purified with water using a 10 mL 7 kDa MWCO Zeba spin desalting column (Thermo Fisher Scientific), and then by dialysis using a 3.5 kDa MWCO regenerated cellulose Spectra/Por®3 dialysis membrane (Spectrum Labs) ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was replaced with DMEM + 10% FBS + CellEvent Caspase-3/7 Detection Reagent (Green) according to the manufacturers recommendations (Invitrogen). The plate was then imaged every 15 minutes using phase and GFP channels in the ZEISS Celldiscoverer 7 Automated Live Cell Imager set to 42°C at 5% CO2 and 20% O2 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1 mL LB and added to a mixture of 2:1 methanol : chloroform (3.5 mL total) in borosilicate glass tubes (16×100 mm, Fisher Scientific 1495935AA). Samples were vortexed for 1 min to form a single phase ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 3.5 μg pMD2.G with Lipofectamine 2000 (Invitrogen) in Opti-MEM media (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Gene expression was determined by qPCR using Brightgreen Dye qPCR master mix on a ViiA 7 Real Time PCR System (Applied Biosystems). Primer sequences were designed using NCBI Primer Blast ...
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bioRxiv - Microbiology 2020Quote: ... The ORFs encoding the MV-C protein and Muc7 protein (Mucin 7 was used as a negative control) were cloned into entry vector pDON207 (Gateway system; Invitrogen, USA). They were transferred into the destination vector pSNL-N2 by Gateway cloning ...
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bioRxiv - Developmental Biology 2021Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems QuantStudio Flex 7 Real Time PCR System (ThermoFisher Scientific). The ΔΔCT method was used for relative quantification of target cDNA abundance representing mRNA expression level normalized to Rn18s (18S rRNA).
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bioRxiv - Cell Biology 2020Quote: ... cells (either MDA MB 231 GFP or a mixture of MDA MB 231 GFP and MCF 7 at a ratio of 1:1) were suspended in cold neutralized bovine collagen solution (2.5 mg/ml) (Thermo Fisher Scientific). This cell suspension was then added to pre-warmed (37 ̊C ...
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bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
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bioRxiv - Cell Biology 2020Quote: ... On day 7 the media was switched to repro media2 and E7 reprogramming media (1: 1) containing E6 basal media (Thermo Fisher) with NaB ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR was performed using PowerUp SYBR Green Gene Expression Assays (Thermofischer A25741) and a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Relative expression values were calculated using the comparative Ct (ΔΔCt ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed using SYBR Green PCR Master Mix (Lifetech, #43-676-59) on a ViiA 7 Real-Time PCR System (Applied Biosystems). The primers used are listed in Table S2 ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR reactions were performed from in vitro cultivated samples with 50ng +RT and –RT cDNA using a ViiA 7 Real-Time PCR system (Applied Biosystems) and Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified with Agencourt AMPure XP beads in 0.7:1 ratio (beads to PCR reaction volume) and quantified with the Quant-iT™ High-Sensitivity dsDNA Assay Kit (Invitrogen). The amplicons were pooled together and sequenced on the Illumina HiSeq 2500 using HiSeq Rapid SBS Kit v2 (500 cycles ...
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bioRxiv - Genomics 2020Quote: ... Experiments were performed in biological triplicates per condition and expression was measured in four technical replicates on QuantStudio 7 (Life Technologies) using TaqMan Gene Expression buffer (Life Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Axial and sagittal sections of the tissue at 7–10 μm thick were then processed and mounted onto cold Superfrost Plus slides (Fisher Scientific) by the PALM Histology Core Facility at the University of Ottawa ...
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bioRxiv - Developmental Biology 2022Quote: ... using KAPA SYBR FAST qPCR Master Mix (2X) ABI Prism (Thermo, KK4617) on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) thermal cycler ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio 7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min followed by glycerol in PBS (volume ratio 7:3) for 30 min before being mounted onto superfrost slides (Fisher Scientific) for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Amplicons from the 7 clones that showed loss of a StyI site upon digestion were cloned into Zero Blunt TOPO (ThermoFisher K280020) and sequenced using primers SP6 and T7 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-ELAV (MAb 7D, 1:20, which recognizes 7 amino acids unique to ELAV) and anti-GFP (1:250; Invitrogen A11122) and visualized with Alexa Fluor 488- and/or Alexa Fluor 647-coupled secondary antibodies (1:250 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR (qPCR) was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system (Applied Biosystems). Expression of genes was normalised to three housekeeping genes (Gapdh ...
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bioRxiv - Molecular Biology 2021Quote: ... and real-time quantitative PCR was performed using a Viia 7 Real-Time PCR System on 384 custom-made TaqMan microfluidic cards (ThermoFisher, 4342253). CTs were normalised to the average CT values of Gapdh and Hprt ...
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bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...