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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Human breast cancer cell lines MDA-MB-231 (ATCC) and MCF-7 (ATCC) were grown in DMEM/F12 (Gibco) with 15 mM HEPES supplemented with 10% v/v fetal bovine serum (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR analysis was performed with a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific) using the ΔΔCt method [50] ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR analysis was performed with a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific) using the ΔΔCt method [50] ...
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bioRxiv - Physiology 2023Quote: ... Quantitative real-time PCR was carried out on a ViiA 7 instrument (Thermo Fisher Scientific, Waltham, MA 02451, USA) using HOT FIREPol Probe mix Universal (Solis Biodyne ...
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bioRxiv - Molecular Biology 2023Quote: ... miRNAs were then quantified using individual TaqMan assays on an ABI QuantStudio 7 real time PCR machine (Applied Biosystems). Assay IDs include ...
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... the transfection mixture was replaced with low-glucose DMEM supplemented with 7% FBS (Gibco BRI; Life Technologies, Gaithersburg, MD). Cells were then cultured for 4-6 days at 30°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: A549 cells grown in 96-well plates were stained with CellEvent Caspase 3/7 green detection reagent (Thermo Scientific) according to manufacturer’s instructions and the fluorescence measured using a plate reader at 488 nm wavelength ...
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bioRxiv - Cell Biology 2024Quote: ... African green monkey kidney fibroblast-like cell line (COS-7) was maintained in minimal essential medium (MEM, Gibco/BRL) supplemented with 5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... thoracic and lumbar DRGs were harvested from 7-to 10-week-old MrgprdGFP mice and transferred to Hank’s buffered saline solution (HBSS) (Invitrogen). Ganglia were treated with collagenase (2 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... Raji B cells were labelled in serum-free medium with 10 μM 7-amino-4-chloromethylcoumarin (CMAC, Thermofisher, USA) for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... SYBR-Green-based real-time quantitative PCR was performed in an ABI QuantStudio 7 Flex machine (Applied Biosystems, USA). Fold-change was calculated by the delta-delta Ct method (Livak & Schmittgen ...
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bioRxiv - Cell Biology 2024Quote: Cells cultured for 4 days at a starting density of 7 x 104/cm2 were lysed in TRIzol (Ambion) for 5 min and transferred to an RNA-free microcentrifuge tube following manufacturer recommendations ...
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bioRxiv - Physiology 2024Quote: ... Approximately 7 µg of tryptic peptide from each tissue sample was labeled with TMT-Pro 16 plex (Thermo Scientific) reagents according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation was monitored every 24 or 72 hr for 3-7 consecutive days using CyQuant Reagent (Invitrogen, C35011) by measuring bottom-read fluorescence at 520 nm with excitation wavelength set at 480 nm using EnSight Multimode Plate Reader (PerkinElmer) ...
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bioRxiv - Cancer Biology 2024Quote: ... and then cultured on Petri culture dishes for 7 days in Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco 12440-053) supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... The Taqman real-time PCR was carried out using Applied Biosystems QuantStudio 7 Flex Real-Time PCR System (Thermofisher), and all the procedures were followed as per the instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Polystyrene beads (diameter of 3.5 µm; S37224, ThermoFisher) were diluted 1:50 in FluoroBrite DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 3.5 % StemPro-34 nutrients (Life Technologies), SCF 100 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... and 3.5 μg/mL laminin (Thermo Fisher Scientific) coated glass bottom plates (CellVis ...
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bioRxiv - Neuroscience 2023Quote: ... and 3.5 μg/mL laminin (Thermo Fisher Scientific)-coated 6 well plates (Greiner ...
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bioRxiv - Microbiology 2022Quote: ... and 3.5 μL of nuclease-free water (Thermofisher), with a total volume of 15 μL per reaction ...
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bioRxiv - Neuroscience 2023Quote: ... and Dispase II (Gibco 17105041; 3.5 mg/mL) in DPBS (Gibco 14190094) ...
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bioRxiv - Microbiology 2023Quote: ... and 3.5 μL of nuclease-free water (Thermofisher), with a total volume of 15 μL per reaction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... assigning loci with GENEMAPPER software 3.5 (Applied Biosystems), and assigning paternity with CERVUS v.3.0 with 99% confidence (Marshall et al ...
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bioRxiv - Biophysics 2021Quote: ... sperm were incubated with 15 μM DEACM-caged cGMP for 7 min in the presence of 0.25% Pluronic™ F-127 (ThermoFisher). Uncaging was performed in the stopped-flow cuvette by a 360 nm LED (Thorlabs ...
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bioRxiv - Biophysics 2021Quote: The proteoliposomes were incubated with buffer IN (100 mM KCl, 20 mM HEPES pH 7, containing either 250 μM Calcein (Invitrogen), 100 μM Fura-2 (ThermoFischer Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... Baculovirus was harvested about 7 days after transfecting bacmid into Sf9 cells cultured in Sf-900 III SFM medium (Gibco) at 27 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time PCR was performed on a high-productivity real-time quantitative PCR ViiATM 7 system (Life Technologies, U.S.A.) using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Cell Biology 2020Quote: ... were injected in the pancreas with 4 ml of collagenase solution (7 ml HBSS, 70 μL HEPES (10 μM) (Gibco), 16,5μL DNAse (10mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative transcript analysis was performed using a QuantStudio 7 Flex Real-Time PCR System with the Power SYBR Green RNA-to-Ct Real-Time qPCR Kit (all ThermoFisher). Total RNA template (25 ng per reaction ...
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bioRxiv - Immunology 2021Quote: ... total bone marrow cells were isolated from the femur and tibias of mice and differentiated into macrophages for 7 days in complete DMEM medium (500 mL, ThermoFisher) supplemented with M-CSF (40 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from pygidia and several posterior segments of young regenerates (7-10 days of regeneration) of Alitta virens and Platynereis dumerilii using TRIzol reagent (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng template was used per reaction with SYBR Green Mastermix in ViiA 7 Real-Time PCR System (Applied Biosystems). As endogenous control ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was then performed for at least 3 biological replicates using Biozym Blue S’Green qPCR Mix Separate ROX (No. 331416XL) on QuantStudio 7 Flex Real-Time PCR System from Applied Biosystems. Pax3 fold change was calculated from ΔCt using Gapdh as housekeeping gene (2^-(ΔCt)) ...
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bioRxiv - Developmental Biology 2021Quote: Micropatterned coverslips with 225-μm diameter synthetic lung buds at day 7 of lung induction were dissociated with TrypLE Express (Gibco) for 10 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... was cultured in Dulbecco’ ss Modified Eagle Medium (DMEM) supplemented with 7% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco) at 37°C with 5% CO2.
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bioRxiv - Immunology 2022Quote: ... was used and results were analyzed by quantitative RT-PCR with a Vii 7 Real-time PCR system (Applied Biosystems). The comparative threshold cycle method and internal control (Gapdh ...
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bioRxiv - Developmental Biology 2020Quote: ... We use the following conditions for the 40-cycle reaction on the Quant Studio TM 7 Flex System (Thermo Fisher): a ...
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bioRxiv - Microbiology 2019Quote: ... Samples with RIN > 7 and DNA contamination <10% were used for cDNA synthesis using SuperScript III first-strand synthesis kit (Invitrogen). RT-qPCR was performed using 2xSYBR FAST qPCR universal MasterMix kit (Kappa Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... an in-house generated model of human hepatic Huh-7 cells stably overexpressing mRFP-Rab5 were cultured in DMEM/GlutaMax/High glucose (Gibco) supplemented with 10 % FBS ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
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bioRxiv - Developmental Biology 2019Quote: ... After 7-10d culture cells were shaken in an orbital shaker at 350-400rpm (1.9cm orbital radius, MaxQ 4450, ThermoFisher Scientific) at 37°C to obtain a culture of cortical astrocytes ...
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bioRxiv - Biophysics 2019Quote: 20-nm fluorescent dark red beads samples (Fig. 2d, Supplementary Fig. 5) were prepared using a 5.10−7 dilution of the initial solution (F8783, Thermo Fisher). We performed the dilution in PBS + 5% glucose to match the index of the dSTORM imaging buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF-7 and MDA-MB-231 cells were plated on glass coverslips in serum-free Gibco OptiMEM medium (Life Technologies) and transduced with 4.8×1010 PFU of Ad5-Laconic (Vector Biolabs) ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were transfected at 7-9 days in vitro (DIV) using Lipofectamine 2000 following the manufacturer’s directions (Thermo Fisher Scientific). A total of 1 μg of DNA was transfected for each well of a 12-well plate for 2-3 hours before switching back to conditioned media ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... of WT and Adrb2-/- Cl4 mice (7-12 weeks old) with a negative isolation kit according to the manufacturer’s instructions (Invitrogen #11417D). Donor T cells were derived from CD90.1 congenic animals in order to track their frequency in CD90.2 hosts ...