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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed using SYBR green reagents on an Applied Biosystems QuantStudio 3 and ViiA 7 Real-Time PCR Systems (ThermoFisher).
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bioRxiv - Cancer Biology 2022Quote: ... and ran on QuantStudioTM 6 Flex Real-Time PCR System using QuantStudioTM 6 and 7 Flex Real-Time PCR software v1.0 (Applied Biosystems). Relative gene expression levels were quantified using β-actin or human TBP as housekeeping genes ...
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bioRxiv - Molecular Biology 2019Quote: ... 250 ng of m1A-containing synthetic oligonucleotide (see Extended Data Table 7) were spotted in triplicate on a BrightStar membrane (ThermoFisher), allowed to briefly air-dry ...
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bioRxiv - Pathology 2020Quote: ... Gene expression by qPCR was performed using a ViiA™ 7 Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). All reactions were performed as biological triplicates and technical duplicates using a GoTaq® qPCR Master Mix Kit (Promega) ...
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bioRxiv - Biochemistry 2019Quote: ... RNAs after hDcp2 treatment were resolved electrophoretically on denaturing 15% acrylamide / 7 M urea 1xTBE gel stained with SYBR Gold (Invitrogen) and visualized using a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... LC-MS/MS data was collected as previously described.7 The raw data files were processed and quantified using Proteome Discoverer software v1.4 (Thermo Scientific) and searched against bacterial genome and horizontally acquired resistance genes as described previously.8
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bioRxiv - Systems Biology 2019Quote: All the reactions were performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems by Life Technologies). Cycling conditions were ...
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bioRxiv - Systems Biology 2019Quote: All the reactions were performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems by Life Technologies). Cycling conditions were ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed on an ABI PRISM 7900HT or QuantStudio 7 Flex Real-Time PCR System (Life Technologies) with Power SYBR Green PCR Mix (Life Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... To measure leukocytosis blood was harvested 7 days post-infection by cardiac puncture into ethylenediaminetetraacetic acid (EDTA) containing 1.7mL tubes (Fisher Scientific) and treated with ammonium-chloride-potassium (ACK ...
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bioRxiv - Physiology 2019Quote: ... Quantitative Real-time PCR (qRT-PCR) was performed in duplicate using the ViiA 7 Real-time PCR system (Applied biosystems). All expression data were normalized by the 2(-ΔCt ...
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bioRxiv - Microbiology 2020Quote: ... The dose-response plates are 200 times diluted in 300μL Phosphate Buffered Saline (PBS; pH= 7-7.2 without Ca/Mg) (Gibco, 14190-144) by transferring 1.5 μL of serially diluted compound ...
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bioRxiv - Physiology 2020Quote: ... and incubated in cell* dishes at 37° C for 4 – 7 hours before adding cell culture medium (RPMI 1640, -glucose +glutamine, Gibco), supplemented with penstrep (10,000 U/ml penicillin / 10,000 μg/ml streptomycin ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... 18.7 mM NH4Cl and 0.2 g/L glucose and supplemented with 20 mL/L MEM essential amino acids (Gibco 11130051), 10 mL/L MEM nonessential amino acids (Gibco 11140050) ...
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bioRxiv - Genomics 2020Quote: ... Quantitative transcript analysis was performed using a QuantStudio 7 Flex Real-Time PCR System with the Power SYBR Green RNA-to-Ct Real-Time qPCR Kit (all ThermoFisher). Total RNA template (25 ng per reaction ...
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bioRxiv - Pathology 2021Quote: ... Quantitative miR expression data were acquired and analyzed using the ViiA 7 real-time PCR system (Applied Biosystems, Cat# 4453545).
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bioRxiv - Pathology 2021Quote: ... and 001234) and ViiA 7 real-time PCR system (Cat# 4453545) were purchased from Applied Biosystems (Foster City, CA, USA). Rabbit polyclonal anti-calnexin (CNX ...
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bioRxiv - Molecular Biology 2021Quote: ... using a 7900HT Fast Real-Time PCR System or a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). The mRNA expression levels were calculated with the 2-ΔΔCt method ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time PCR was performed and analysed using the ABI ViiA™ 7 real time PCR system (Thermo Fisher Scientific). TaqMan Gene Expression Assays (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2020Quote: ... African Green monkey kidney epithelial cells) and Huh-7 cells (human hepatocellular carcinoma cells) were cultured in Dulbecco’s minimal essential medium (DMEM; Life Technologies), supplemented with 10 % fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... Digestion was stopped by addition of an 8.6% SDS/7 mM EDTA solution and crosslinks were reversed by adding Proteinase K (20 mg/ml; Life Technologies) and incubating 6 hr at 65°C ...
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bioRxiv - Cell Biology 2019Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (AL007A, HiMedia, Mumbai, Maharashtra, India) supplemented with 10% FBS (10270-106, GIBCO-Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and from day 7 cells were fed with fresh RPMI medium supplemented with B27 with insulin (#17504044, Thermo Fisher Scientific) without extra supplement ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 18.7 mM NH4Cl and 0.2 g/L glucose and supplemented with 20 mL/L MEM essential amino acids (Gibco 11130051), 10 mL/L MEM nonessential amino acids (Gibco 11140050) ...
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bioRxiv - Cell Biology 2020Quote: ... FACS analysis of transfected BEAS-2B cells for caspase 3 & caspase 7 along with SYTOX AADvancedTM Dead Cell Stain was performed per manufacturer’s protocol (Thermo Scientific).
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bioRxiv - Plant Biology 2021Quote: ... primed reverse transcription of first-strand cDNA synthesis was carried out with 7 mg total RNA using Super ScriptTM III (Invitrogen). Equal volumes of each first-strand reaction were amplified with gene-specific primer pairs ...
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bioRxiv - Biochemistry 2021Quote: 3 biological replicates of wild type and 2 different clones METTL5-KO HeLa cells (clone 1 and 7) were grown to confluency prior to lysis in Trizol (Invitrogen). Total RNA was purified as described above (‘RNA purification’ ...
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bioRxiv - Physiology 2021Quote: ... JC-1 dye was added 10 min prior to recording (ex.488/em.535 and em.590) while CellEvent Caspase 3/7 (ThermoFisher) was added immediately prior to recording (ex.495/em.540).
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bioRxiv - Microbiology 2019Quote: ... was used to quantify the relative levels of cells using a Viia™7 Real-Time PCR Instrument (Applied Biosystems) with 50 ng gDNA template using the following primers ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane and analyzed by western blotting using an anti-His tag antibody (7) or stained with Krypton fluorescent dye (ThermoFisher) according to a company’s instruction ...
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bioRxiv - Bioengineering 2021Quote: Spleens and injection dLNs were collected on day 28 (7 days post-boost) and stored in ice-cold IMDM (Gibco) until further steps ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected into cultured neurons at DIV 7-9 using a calcium phosphate transfection kit (Thermo Fisher, K2780-01). Experiments were performed 2 or 3 days post transfection.
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bioRxiv - Bioengineering 2021Quote: ... Culture medium from day 0 until day 7 was RPMI1640 (HyClone, SH30027.01) plus B-27 serum-free supplement without insulin (Gibco, A1895601). From day 7 and onwards RPMI1640 with B-27 serum free supplement with insulin (Gibco ...
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bioRxiv - Pathology 2021Quote: ... 35 mins in MES buffer for aSyn detection) and transferred to nitrocellulose membranes (0.2 μm) via Iblot (7 mins, 20 mV; Fisher Scientific). Initial blots were tested for optimisations of aSyn retention as labelled by MJFR1 either by pre-blocking heat treatment (microwaved ...
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bioRxiv - Immunology 2021Quote: ... 1 μg/ml -CD28 and 1:50 diluted conditioned supernatant from COS-7 cells transfected with a pcDNA1 vector (Invitrogen) encoding for recombinant chicken IL-2 (a kind gift from prof ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated with 7 µl of resuspended RNA using SuperScript™ III First-strand synthesis system (Thermo Fisher Scientific), and primers A1 and A2 (Table 1 ...
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bioRxiv - Immunology 2020Quote: Cell survival was determined by analyzing forward/sideward scatter properties of cells and simultaneous staining with either Propidium iodide (PI), or 7-amino-Actinomycin D (7AAD eFluor670 dye, and LIVE/DEAD fixable violet stain (ThermoFisher), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of these genes was performed using SYBR Green on a ViiA™ 7 sequence detection system (Applied Biosystems, USA) with two replications for each sample ...
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bioRxiv - Immunology 2021Quote: ... NKp46+ and stained as indicated by manufacturer’s protocol with Cell Event Caspase 3/7 Green Flow Cytometry Assay Kit (Molecular Probes). All samples were analyzed using BD Canto FACS instrument ((BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 2019-nCoV CDC RUO Kit (Integrated DNA Technologies) and QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). PCR amplicons were quantified against in vitro transcribed RNA N gene fragment standard ...
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bioRxiv - Immunology 2020Quote: PBMC were seeded at a concentration of 7-8 × 106 cells/mL in RPMI 1640 medium without phenol red and glutamine (Gibco) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Genomics 2021Quote: ... Apoptosis staining was performed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific, Waltham, MA). Stained cell suspensions were measured with the BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and second strand synthesis performed on the slide with cDNA quantification undertaken with qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA systems) and analysed on the QuantStudio 7-Flex system (ThermoFisher). FFPE samples were cut at the standardised 5 µm thickness onto four 6.5mm x 6.5mm capture areas ...
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bioRxiv - Immunology 2022Quote: ... The amplification reaction was monitored on the ViiA 7 Real-Time PCR System running on the QuantStudio Real Time PCR Software V1.3 (Thermo Fisher). The size of the respective amplicons was confirmed by 2% agarose gel and ethidium bromide staining ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640; Gibco) supplemented with penicillin/streptomycin and 10% FCS ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and COS-7 cells were maintained at 37 ºC and 5% CO2 and cultured in Dulbecco’s modified Eagle media (Life Technologies) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Cell Biology 2022Quote: Human breast cancer (MCF-7) cells were obtained from ATCC and cultured every two days in Minimum Essential Medium (MEM) Alpha media (Gibco) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Cell Biology 2022Quote: Lipid overlay assay was performed using PIP strips (nitrocellulose membrane spotted with 8 phosphoinositides and 7 other biologically relevant lipids) according to the manufacturer’s instructions (Thermo Scientific). Briefly ...