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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1, 3, and 7) with 200 µl Trizol (Invitrogen) per sample and stored at −80 °C until sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2020Quote: The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Biochemistry 2021Quote: ... cell pellets were incubated with 300 µL of 10 mg/mL lysozyme (prepared fresh in sterile H2O) and incubated for 7 minutes at 30°C/250 rpm to allow for efficient lysis by TRIzol reagent (Ambion). 10-15 glass beads (4mm diameter ...
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bioRxiv - Bioengineering 2021Quote: ... All proteins were purified with desalting columns (Zeba Spin Desalting Columns, 0.5 mL with 7 kDa MWCO, Thermo Fisher Scientific) by washing with PBS three times (centrifuging 1500 rcf ...
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bioRxiv - Bioengineering 2020Quote: ... A biphasic solvent overlay was employed to capture patchoulol by adding 7 mL of filter-sterilized n-dodecane (Acros Organics) to each culture (10% v/v) ...
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bioRxiv - Bioengineering 2020Quote: ... The samples were sectioned using Kawamoto’s cryofilm type 2C (SECTION-LAB Co. Ltd., Japan) to 7 µm thickness using a cryotome (CryoStar NX70, Thermo Scientific) according to the Kawamoto protocol [48] ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from HTM cells ± DEX at 7 d (N = 3 per group and donor) using PureLink RNA Mini Kit (Invitrogen). RNA concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Biochemistry 2020Quote: MCF-7 cells (150–1000 cells/well) were plated in Labtek Permanox Plastic Chamber slide system (Nunc, Thermo; cat. #177445) and incubated overnight ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Fractions containing purified protein were pooled and dialyzed overnight against storage buffer (0.02 M sodium phosphate, pH 7) in a 10 kD cut off dialysis cassette (ThermoFisher, 66380). Purified protein was stored at 4 °C for use within days or at −20 °C for longer-term storage.
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bioRxiv - Cancer Biology 2020Quote: ... and the doxycycline-inducible COV434 pIND20 BRG1-2.7 and TOV21G pIND20 ARID1A (generously donated by Dr. Bernard Weissman, University of North Carolina) were cultured in DMEM (Gibco) and supplemented with 10% TET free FBS (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... Eyes were first incubated in 1 mL of high glucose Hanks balanced salt solution/0.5 mg/ml proteinase K (37°C, 7 min) and then in Neurobasal medium (Life Technologies) plus 10% fetal calf serum to stop enzymatic activity ...
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bioRxiv - Plant Biology 2021Quote: ... pistils and young seeds (7 DAP from CT and 5 DAP from HT) were isolated following a protocol for Trizol (Invitrogen). RNA from 26 DAP seeds was isolated using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2020Quote: ... qPCRs were carried out on an Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Applied Biosystems, Foster City, California, USA) using isolated genomic DNA as template ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cultures were grown at 37°C either in M9 media [M9 salts (248510, Difco)] supplemented with 0.4% glucose (6363-53-7, Fisher Scientific), 1 mM MgSO4 (Sigma Aldrich) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A melting curve was generated by monitoring fluorescence at 465 nm/610 nm excitation/emission wavelengths while increasing temperature from 10 °C to 90 °C at a rate of 0.06 °C/s on an Applied Biosystem ViiA 7 instrument (Life Technologies). To calculate Tm values ...
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bioRxiv - Microbiology 2021Quote: ... and qPCR were performed on 5µL of the cDNA libraries diluted at 1:10 using the Precision Plus 2x qPCR Mastermix (Primer Design) according to the manufacturer’s instructions and the Quant Studio 7 Flex (Applied Biosystems). For validation of the translatomic results ...
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bioRxiv - Cell Biology 2020Quote: Cos-7 cells were maintained in DMEM + 10% fetal bovine serum and all transfections were conducted using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 100-µl drop of 0.5% low-melting agarose (FMC, Fig. 1D,E, Fig. S1G) containing a 6:7 ratio of spinal cord medium [MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR was performed using 384-well plates (4titude Ltd, UK) in the ViiA™ 7 System (Applied Biosystems, UK) with the SensiFAST™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... Strip tubes were loaded onto the Genie-II or Genie-III (OptiGene Ltd) and 96-well plates run on a QuantStudio 7 thermocycler (ThermoFisher). Reactions were incubated at 65°C for 30 min with fluorescence acquisition every 30 sec (Genie instruments ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions with RNase T2 were done at room temperature for 10 minutes in 1× Structure Buffer (10 mM Tris pH 7, 100 mM KCl and 10 mM MgCl2; Ambion). Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x 107 MRC5 cells were infected as described above and total RNA was isolated from cells using TRIzol (Invitrogen), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell death was assessed by measuring caspase 3 cleavage using a CellEvent Caspase3/7 Green Flow Cytometry kit (Thermo Fisher), according to the manufacturer’s descriptions ...
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bioRxiv - Cancer Biology 2020Quote: ... was mixed with Taqman assays and analysed in a 384 well QuantStudio Flex 7 (Applied Biosystems Life Tech, Thermo Scientific) in 5μl reaction volumes ...
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bioRxiv - Cancer Biology 2020Quote: ... was mixed with Taqman assays and analysed in a 384 well QuantStudio Flex 7 (Applied Biosystems Life Tech, Thermo Scientific) in 5μl reaction volumes ...
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bioRxiv - Cell Biology 2021Quote: ... The quantitative PCR (Q-PCR) reactions were conducted in triplicate on a ViiA 7 Real-Time PCR system (Thermo Fisher) from 1 μL of cDNA using the PerfeCTa SYBR Green SuperMix reagent (Quantabio Cat# ...
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bioRxiv - Cell Biology 2021Quote: ... The tissues were then digested in room temperature for 7 min in a 3-mL Hank’s solution containing 0.25% Trypsin (Thermo Fisher, 15090046) and 0.1 μg/μL DNase (Sigma D5025 ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher Scientific, 89960; IL). The original murine anti-PD1 monoclonal antibody (63 ...
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bioRxiv - Genetics 2022Quote: ... The volume was filled up to 7 ml with water and 50 units of T4 DNA ligase (Thermo Fisher Scientific) were added ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... and qPCR was performed on 5μL of the cDNA libraries diluted at 1:5 using the Precision Plus 2x 598 qPCR Mastermix (Primer Design) according to the manufacturer’s instructions and the Quant Studio 7 Flex (Applied Biosystems).
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bioRxiv - Immunology 2022Quote: pDC generation: bone marrow cells were isolated (see above) and cultured for 7 days in RPMI-1640 GlutaMAX-I (GIBCO) supplemented with 10% FCS (GIBCO) ...
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bioRxiv - Neuroscience 2022Quote: Differentiated cells on day 15 (8 days suspension culture plus 7 days adherent culture) were harvested and dissociated using TrypLE Select (Gibco). Cells were then passed through a 20 µm strainer and resuspended in 1 x PBS with 0.04% BSA buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 350ng of pCAGIG-VENUS was used to transfect the neurons two days after adding mouse glia (PNI day 7) using Lipofectamine 2000 (ThermoFisher). A full media change using conditioned media was performed 5-6 hours post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec) in a QuantStudioTM 7 Flex Real-Time PCR System (Life Technologies). Protein thermal melting curve were generated using the Protein Thermal Shift TM software ...
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bioRxiv - Neuroscience 2022Quote: ... neural progenitor cells were manually isolated from rosettes and expanded as neurospheres in a suspension culture for 7 days in E6N2 supplemented with 50x B27 lacking retinoic acid (Invitrogen) and 20 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Neuroscience 2022Quote: ... jGCaMP7b-XC or jGCaMP7b-XN and 1 μg cDNA encoding CFP (for labelling the soma area and neurites) were transiently transfected into DIV 5-7 cultured cortical neurons by Lipofectamine 2000 (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Microbiology 2022Quote: ... as per manufacturer’s instructions in 96-well plates and conducted in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Primers were designed using Primer3 94 to target the hucL gene (HucL_fw ...
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bioRxiv - Biophysics 2022Quote: ... The diluted sample was then passed through a Zeba Spin Desalting Column (7 kDa cut-off, Thermo Fisher Scientific, France) equilibrated in acquisition buffer containing 0.005% LMNG (w/v) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted from rats aged postnatal day 5-7 and placed in working buffer (Hank’s balanced salt solution (HBSS) (Life Technologies) with 0.5% chromatographically purified bovine serum albumin (BSA ...
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... 2015) (Supplemental Table 6 and 7) junctions and a high-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, USA) and Sanger sequencing (Solgent ...
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bioRxiv - Microbiology 2021Quote: ... The number of PCR cycles for sufficient DNA for downstream applications was empirically determined by quantitative PCR (ViiA 7 Real-Time PCR System, ThermoFisher) in the presence of 1X Evagreen (20X Evagreen ...
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bioRxiv - Cancer Biology 2020Quote: ... Reactions were performed using the pre-set program of the ABI ViiA 7 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... marrow was extracted from femurs and tibias of 8 to 12 week-old male and female mice and differentiated for 7 days into BMM in Dulbecco’s Modified Eagle’s Medium with glutamine (DMEM, Thermo Fisher Scientific), containing 10% heat-inactivated ...
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bioRxiv - Microbiology 2022Quote: ... targeting a region in the viral ORF1ab gene and using a QuantStudio™ 7 Real-Time PCR System (Applied Biosystems). Data were processed using GraphPad Prism software (V8.0 ...
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bioRxiv - Genetics 2022Quote: ... were combined and concentrated to ∼1 mL and dialyzed using 7 KMWCO Snake Skin dialysis tubing (Thermo Scientific, Waltham, Massachusetts) into 20 mM Tris 150 mM NaCl pH 8.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Real-time quantitative PCR was performed on a ViiA 7 Real-Time PCR System with OptiFlex Optics System (Applied Biosystems) using PowerUp SYBR Green PCR kit (Applied Biosystems) ...