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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Following EDTA dissociation crypts were incubated at 37°C for 7 minutes in TrypLE Express (Thermo Fisher Scientific, 12-604-021) and DNase I (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... proteins were dialyzed overnight using a 7 or 10 kDa molecular weight cut-off Slide-A-Lyzer dialysis cassette (Catalog No. 66380, Thermo Scientific) in high salt buffer (500 mM NaCl and 20 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR reactions (all samples are run in triplicate) were carried out on the QuantStudio™ 7 Flex system (Applied Biosystems), and results were calculated by the delta-delta Ct (ΔΔCt ...
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was run in triplicates using 2-5 ng cDNA in a 384 well plate and the qPCR was performed using VIIA 7 real time PCR instrument from Applied Biosystems.
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bioRxiv - Cancer Biology 2023Quote: Cell viability in untreated tumor organoids was analyzed on day 7 using the LIVE/DEADTM Cell Imaging Kit (Invitrogen, Karlsruhe, Germany) according to the manufactureŕs instructions for exemplary cases.
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Genomics 2023Quote: The RT-qPCR reactions were carried out on 384-well plates using a QuantStudio 7 Flex Real- Time PCR System (ThermoFisher #4485701) using 10 µl reactions (2 µl of cDNA 3.7 ng/µl ...
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bioRxiv - Plant Biology 2023Quote: Total protein isolated from the light-grown 7-day-old seedlings of each sample using a plant protein extraction kit from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... BAL samples were then centrifuged at 3,000 g for 20 min to collect cells and ApoBDs for staining with a combination of SR-DEVD-FMK (caspase 3/7 activity detection dye, Thermo Fisher), annexin A5-V450 and TO-PRO-3 in 1× A5 binding buffer at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: 500,000 MDA-MB-231 cells plated in a six-well dish were transfected with 20 nM of the appropriate siRNA (IDT) or 20 nM of appropriate LNA diluted in OPTI-MEM containing 7 µL of RNAiMAX (Thermo Fisher). Transfections were performed according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The medium was changed every day and cells were passaged at 70%-80% confluency every 5-7 days using PBSCa-/Mg- (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was performed with 2.5 ng of DNA in triplicates using PowerSYBR Green PCR master mix at Quant Studio 7 qPCR machine (both Applied Biosystems) according to the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... Hippocampi of male and female E17 mouse brains from a single litter (7-9 embryos) were dissected in HBSS (ThermoFisher Scientific) supplemented with 1% L-glutamine ...
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bioRxiv - Genetics 2023Quote: ... The qPCR experiments were performed in triplicate in a ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Proliferation was assessed 7 days after treatment with respective drugs using CellTiter-Glo® luminescent Cell Viability Assay (Fisher Scientific, PRG9242). The luminescence signal was measured in a luminometer ...
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bioRxiv - Developmental Biology 2023Quote: ... or Blue ŚGreen qPCR kit (Byozim) on a 7900HT Real Time PCR system or QuantStudio 7 Flex Real-Time-PCR-System (Applied Biosystems). Primer sequence information is provided in supplementary table 4 ...
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bioRxiv - Biophysics 2023Quote: ... The cell-culture medium contained a 1:1 mixture of Eagle’s minimal essential medium (EMEM; for MCF-7) and F12 (Invitrogen, Waltham, MA) with a 2% B-27 supplement (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... and normal tissues were mechanically cut into 3-7 mm fragments and incubated in RPMI 1640 medium (Gibco™, cat. 42401042) containing 1 mg/ml Liberase TL Research Grade (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... beads were resuspended in 20 µl of Elution Buffer mixed with 7 µl of 4X LDS sample buffer (Thermo Fisher, # NP0007) and 3 µl of 10X reducing agent (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... cells were reverse-transfected into an arrayed 384- well plate library containing small interfering RNA (siRNA) or miRIDIAN microRNA mimic constructs (Horizon Discovery, Supplementary Tables 1-7) using Opti-MEM (Gibco 31985070) and Lipofectamine RNAiMAX transfection reagent (Invitrogen 13778150) ...
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bioRxiv - Molecular Biology 2023Quote: ... Movies were collected on a Titan Krios G3i at spot size 7 with a Falcon 4 Direct Detector (Thermo Fisher Scientific) equipped with an energy filter set to 20 eV ...
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bioRxiv - Developmental Biology 2024Quote: ... Real-time PCR amplification and fluorescence detection were carried out using a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher). An initial step (95 °C ...
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bioRxiv - Genomics 2024Quote: ... was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system using standard settings (Applied Biosystems). Expression of genes was normalised to two housekeeping genes (Bactin ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... subsequently buffer exchanged by using 7 K MWCO Zeba Spin Desalting Columns and the concentration was measured by Nanodrop One (Thermofisher Scientific).
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bioRxiv - Biophysics 2023Quote: 7 μM Aβ40 was incubated with varying stoichiometries of claramine and 5% PEG (50% w/w 10 kDa, Thermo Fisher Scientific) in the presence of 20 mM ThT (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... Gene expression was quantified with an SYBR Green real time-PCR using the ViiA™ 7 system (4453535, Thermo Fisher Scientific). β-Actin was used as a housekeeping gene to normalize the amounts of cDNA in each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 mM sodium citrate pH 5.0 buffer containing 7 M urea and 10.5 mM EDTA with 1 U RNase T1 (ThermoFisher Scientific, Waltham, MA) at 55°C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the CSF or plasma SEC fractions 7-10 was then determined using Qubit Protein Assay kit (ThermoFisher Scientific) using 1 μL of sample ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (4368708, ThermoFisher Scientific). Primers for HA (5’- AAACTCTTCGCGGTCTTTCCA-3’ sense sequence and 5’-GATAAGGTAGCTTGGGCTGC-3’ antisense sequence) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 99 °C with shaking for 7 min and 25 μg of protein per sample was analysed with 4-12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... were removed from the culture membrane AT 7 DIV with a scalpel blade into pre-filled bead mill tubes (Fisherbrand: 15555799) containing RNAlater stabilisation solution (ThermoFisher: AM7020) and kept at 4°C until RNA extraction ...
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with solution containing 468 μl of 50 mM ammonium bicarbonate and 7 μg of Trypsin (Thermo Fisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
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bioRxiv - Cancer Biology 2024Quote: ... Biozym Scientific GmbH) was used and analysis was done on a ViiA 7 Real-Time PCR System (4453536, Thermo Fisher Scientific). mRNA levels were normalized to the geometric mean of Cyclophilin A and hypoxanthine guanine phosphoribosyl transferase and/or 18S ...
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of a 1:10 dilution of 1st strand cDNA was assayed by qPCR on Applied Biosystems Viia-7 instruments in 10 µL reactions with PowerUp SYBR Green master mix (Applied Biosystems) according to manufacturer recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (qPCR) was performed using standard SYBR green reagents and protocols on a QuantStudio 7 Real-Time PCR system (Applied Biosystems). All reactions were performed in technical triplicates ...
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bioRxiv - Cell Biology 2024Quote: COS-7 cells were grown at 37°C with 5% CO2 in complete Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific, USA) containing 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: The procedure for cloning KDR CDS was as described before but with the use of the primers listed in Supplemental Table 7 and cloned into the Gateway vector pDONR221 (Thermo Fisher Scientific). The competent cells transformed with the entry vectors were selected for growth on the antibiotic kanamycin (50 µg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was further used for Real-time quantitative polymerase chain reaction (qPCR) performed on QuantStudio 7 Flex system (Invitrogen™ Life Technologies) using Fast SYBR Green Master Mix (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis was performed by quantitative real-time RT-PCR (qRT-PCR) using SYBR Green PCR Master Mix and the ViiA™ 7 Real-Time PCR System (Applied Biosystems). For each target gene ...
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bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).
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bioRxiv - Genetics 2021Quote: ... Blocks were later cut on a cryostat and 7-10 μm thick sections were placed on SuperFrost Plus slides (Cat#FT4981GLPLUS; ThermoFisher; Waltham, MA). Hematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Genetics 2021Quote: ... The purified products were separated and detected by capillary electrophoresis (CE) using the ABI 3130 Genetic Analyzer with POP-7 polymer and GeneScan-120 Liz (Thermo Fisher Scientific). The raw data were analyzed using the GeneMapper ID V3.2 software (Thermo Fisher Scientific) ...