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Citations for Thermo Fisher :
2001 - 2050 of 7360 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Curli fibers were detected via direct fluorescent immunostaining with an α-6X-His antibody (Invitrogen) conjugated to a fluorescent dye ...
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bioRxiv - Synthetic Biology 2021Quote: ... Ni-NTA coated magnetic beads (Dynabeads™ His-Tag Isolation and Pulldown from Invitrogen, USA) were added to the solution and incubated for 10 additional min in order to bind the his-tagged TDF and PKnG proteins ...
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bioRxiv - Bioengineering 2020Quote: ... Isolated PBMCs were then cryopreserved in solution containing 90% heat-inactivated FBS (HI-FBS) (ThermoFisher) and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Systems Biology 2019Quote: ... The His tag was cleaved by overnight incubation at 4 °C with SUMO protease (Invitrogen), after which the sample was loaded again onto a HisTrap FF column to recover the cleaved products ...
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bioRxiv - Developmental Biology 2020Quote: ... and verified by Western blot with primary antibodies against 6x His tag (Invitrogen, MA1-21315), and DLX1 (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... This synthetized gene was cloned into the Champion™ pET302/NT-His expression vector (Thermofisher) via EcoRI and XhoI restriction sites ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme and Fc-portion was removed on HIS-Pur Ni-NTA resin (Thermo Fisher Scientific) and Protein G sepharose (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Anti-6x(HIS) anti-mouse antibody was used to recognize VHH (4A12E6: Invitrogen Rockford, USA) followed by probing with anti-mouse IgG raised in goat (A3562 ...
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bioRxiv - Cell Biology 2020Quote: ... the constructs were all based on a pcDNA3.1 vector containing a V5/His-tag (Invitrogen). Untagged constructs for comparison were prepared by introducing a stop codon into the pcDNA3.1/V5-His constructs using the same site-directed mutagenesis kit as above ...
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bioRxiv - Microbiology 2022Quote: ... Then 1 μl of Dynabeads™ His-Tag Isolation and Pulldown magnetic beads (ThermoFisher Scientific) were added into each well ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% Tween-20) and added to 20 μl Dynabead His-Tag beads (ThermoFisher Scientific; 10103D). After incubation on a roller at 4°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... VarG-HIS bands were excised and diluted with NuPAGE LDS sample buDer (Invitrogen, Carlsbad, CA) mixed with10 mM dithiotreitol (DTT ...
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bioRxiv - Biochemistry 2022Quote: ... each with an N-terminal (His)6 tag from plasmids generated by GeneArt (ThermoFisher Scientific). For each purification ...
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bioRxiv - Immunology 2022Quote: ... containing a hexa-His tag was purified using HisPur Ni-NTA resin (Thermo Fisher Scientific). Expi cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Neuroscience 2022Quote: ... using an Ion PGM Hi-Q View Sequencing Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2023Quote: ... containing 10% (v/v) heat-inactivated fetal bovine serum (HI-FBS; Thermo Fisher Scientific, 10099), penicillin (100 U/ml) ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 10% (v/v) heat-inactivated fetal bovine serum (HI-FBS; Thermo Fisher Scientific, 10099), penicillin (100 U/ml ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Immunology 2023Quote: ... Glycan samples were run with a LIZ 600 DNA ladder in Hi-Di formamide (ThermoFisher) on an ABI 3500xL DNA sequencer and analyzed with GlycanAssure Data Acquisition Software v.1.0 ...
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bioRxiv - Genetics 2023Quote: ... This PCR fragment was TOPO cloned into pcDNA™3.1/V5-His backbone (Invitrogen, V81020). We used in vivo assembly cloning 40,41 for site directed mutagenesis to modify the nucleotide 1 bp upstream of the exon 49 splice junction to each of the alternative nucleotides (Supplementary table 1) ...
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bioRxiv - Biophysics 2023Quote: ... His-tag purification was performed in Pierce disposable polypropylene 5mL disposable columns (Thermo Fisher Scientific) with a wash solution containing TNi 100/300/20 (100 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... The coding region for Hsc70-4 (CG4264) was cloned into pAc5.1/V5-His A (Invitrogen), and into pcDNA™6/myc-His A (Invitrogen) ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... with the Ion PI™ Hi-Q™ Chef Kit (Thermo Fisher Scientific, Catalog # A27198) and Ion PI™ Chip Kit v3 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... or F-vec were cultured in DMEM + 10% HI FBS with Zeocin (Thermo Fisher Scientific) selection ...
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bioRxiv - Molecular Biology 2023Quote: ... One microliter of sample was diluted in 9 ul Hi-Di™ Formamide (Applied Biosystems) containing 0.25 ul GeneScan™ 600 LIZ™ Size Standard ...
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bioRxiv - Neuroscience 2023Quote: ... rattus Piccolo (2622-2937) DNA was cloned into vector pCDNA3.1-myc-his(C) vector (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... The pIgR gene was inserted into the pEF-Myc-His vector (ThermoFisher Scientific, MS, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... The PVDF membrane was then incubated with anti-his mouse primary antibody (Thermo Scientific Pierce) in TBS-T (1:5000 ...
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bioRxiv - Developmental Biology 2023Quote: ... We also subcloned this cassette into a pAc5.1/V5-His A plasmid (Invitrogen #V4110-20) by cutting the cassette out of pUASP-attb-Cry2-CTEV-mCherry using NotI and XbaI (NEB #R3189S and #R0145S ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved in heat-inactivated fetal-bovine serum (HI-FBS, ThermoFisher Gibco™ #12484028, USA) + 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved in heat-inactivated fetal-bovine serum (HI-FBS, ThermoFisher Gibco™ #12484028, USA) + 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B) ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Molecular Biology 2023Quote: ... which were pre-loaded with 10 μL of Hi-Di™ formamide (ThermoFisher, Waltham, USA) and GeneScan™ -400HD ROX™ Size standard (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged LCRIS_00661 and LCRIS_00558 genes with the appropriate restriction sites were ordered from Invitrogen and subcloned into a previously constructed S ...
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bioRxiv - Microbiology 2024Quote: Dynabeads™ His-Tag Isolation and Pulldown magnetic bead solution (Thermo Fisher Scientific, United States) kit was used for the detection of the binding partner of OipA ...
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bioRxiv - Cell Biology 2024Quote: ... Western blotting was performed using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) and GST monoclonal mouse antibody (own antibody) ...
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bioRxiv - Bioengineering 2019Quote: Human embryonic kidney (HEK) 293T cells were obtained from ATCC and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Life Technologies 11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK)-293T cells in log-phase growth were rapidly transfected by using the standard Lipofect Amine reagent (Invitrogen), with GFP-Vector plus helper plasmids to develop virions (Cenciarelli et al. ...
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bioRxiv - Immunology 2019Quote: ... Human embryonic kidney cell-line 293T (HEK 293T) were transfected with the expression vector construct pSegTag2-Emact or the empty pSecTag2 vector (Invitrogen) as control ...
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bioRxiv - Genetics 2021Quote: Low-passage HEK 293FT cells were grown in DMEM supplemented with 10% FBS (D10 media) and passaged using TrypLE (Gibco). For viral plasmid transfection ...
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bioRxiv - Immunology 2021Quote: ... the WNV replicon and C-prM-E plasmids were co-transfected into HEK-293T cells using Lipofectamine 3000TM transfection reagent (Invitrogen). Transfected cells were incubated at 30°C and RVP-containing supernatants were harvested 3-6 days post-transfection and pooled ...
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bioRxiv - Neuroscience 2020Quote: ... One fifth of the HEK-T293 culture was split into two petri dishes of 6cm diameter (Falcon) and transfected with lipofectamine (Invitrogen) and 8µg of either pUb6-tdTOM (i.e. ...
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bioRxiv - Bioengineering 2021Quote: ... and human embryonic kidney (HEK 293T) (ATCC, CRL-3216) cell lines were cultured in high-glucose Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 12634010) media supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HTB-96), and HEK-293T (ATCC, CRL-3216) cells were purchased from ATCC (Manassas, VA, USA) and cultured in DMEM (GIBCO, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... together with the helper plasmids pHelper 1.0 (Gag and Pol) and pHelper 2.0 (VSVG) were transiently cotransfected into HEK 293T cells using Lipofectamine 2000 (Invitrogen, USA) to package lentivirus particles ...
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bioRxiv - Neuroscience 2022Quote: HEK cells were transfected with several Mover constructs one day after plating using PEI transfection in Opti-MEM medium (Gibco). Per coverslip ...
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bioRxiv - Genomics 2022Quote: ... MDA-MB-231 and HEK-293T cell RNAs (5 μg) were incubated with 2 U TURBO DNase for 30 min at 37 °C (ThermoFisher). After DNA digestion ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293T (HEK 293T) cells (ATCC CRL-3216) were cultured at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Neuroscience 2019Quote: ... Media for HEK cells stably expressing NaV1.1 or NaV1.6 also contained 400 μg/ml G418 (Fisher Scientific 10-131-035) to select for transfected cells ...