Labshake search
Citations for Thermo Fisher :
1851 - 1900 of 7360 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... His-tagged hAgo2 was purified from Sf9 cells using a baculovirus system (Thermo Fisher). Cells were lysed in Lysis Buffer (300mM NaCl ...
-
bioRxiv - Microbiology 2022Quote: Pull-down assays were carried out using Dynabeads His-tag Isolation and Pulldown (Invitrogen). The 6His-tagged soluble pilin domains were used as bait ...
-
bioRxiv - Biochemistry 2022Quote: ... and cultured in house) were grown to 70% confluency in DMEM-Hi glucose (Gibco) supplemented with 10% heat-inactivated foetal bovine serum (Sigma) ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were digested and ligated into pcDNA3.1 myc-His B expression vectors (Invitrogen), with a 3xFlag epitope tag also fused to the N-terminus of Ptchd1 ...
-
bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2022Quote: ... redundant anti-His antibody was washed away and 15 μL chemiluminescence substrate (Thermo Scientific) was added onto each well to generate chemiluminescence signal ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
-
bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Junction PCR was performed with Extensor Hi-Fidelity PCR Master Mix (Thermo Fisher Scientific) in a 20 μL reaction using 500 nM of target-specific primers and the following cycling conditions ...
-
bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
-
bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
-
bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
-
bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
-
bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
-
bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
-
bioRxiv - Cell Biology 2022Quote: ... Full length Cavin1 and ΔDR1 mutants were expressed and purified from suspension cells HEK-293F (Invitrogen, Carlsbad, CA, USA) as described previously (33) ...
-
bioRxiv - Cancer Biology 2020Quote: HEK-293T cells were plated at a density of 1.0 × 106 cells/10 cm plate in RPMI 1640 (Gibco) supplemented with 10% heat-inactivated FBS ...
-
bioRxiv - Biochemistry 2019Quote: Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose (Gibco)) supplemented with 10% (v/v ...
-
bioRxiv - Developmental Biology 2019Quote: HEK cells were grown in 6 well plates and transfected with 4µg of each DNA with lipofectamine 2000 (Invitrogen) for 6hrs according to manufacturers instructions.
-
bioRxiv - Bioengineering 2019Quote: HEK 293T cells (authenticated by Bio-Synthesis, Inc. and tested negative for mycoplasma using a kit from Molecular Probes) were plated at low density on poly-L-lysine coated coverslips (18 mm ...
-
bioRxiv - Cancer Biology 2020Quote: RPE1-hTERT and HEK 293T cell lines were purchased from ATCC and cultured in DMEM (41966052; Thermo Fisher Scientific) containing 10% fetal bovine serum (10500064 ...
-
bioRxiv - Neuroscience 2020Quote: HEK 293T cells were cultured at 37°C in 5% CO2 in DMEM+ GlutaMax (Thermo Fisher, cat. no. 10566016) with 10% fetal bovine serum (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... pcRV-Gagpol and pMD2G vectors into HEK-293T cells with lipofectamine 3000 according to manufacturer instructions (Thermo Fisher, LF300001). Target cells were infected with lentivirus supplemented with 5µg/ml polybrene (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... HEK cells were transfected using Lipofectamine 2000 according to different siRNA quantity in Opti-MEM medium (Thermo scientific, USA). The transfected cells were infected with either CHIKV strains PS or IS with MOI 0.1 at 24 hours post transfection (hpt) ...
-
bioRxiv - Molecular Biology 2020Quote: HeLa and HEK-293T mammalian cell lines were maintained in RPMI and DMEM-High glucose (Gibco, ThermoFisher Scientific, USA) media ...
-
bioRxiv - Molecular Biology 2020Quote: HeLa and HEK-293T mammalian cell lines were maintained in RPMI and DMEM-High glucose (Gibco, ThermoFisher Scientific, USA) media ...
-
bioRxiv - Immunology 2022Quote: Proteins were expressed by transient transfection of HEK 293F cells (0.8-1.2 million cells/mL) maintained in Freestyle medium (Life Technologies). A 3:1 ratio of PEImax and expression plasmids (312.5 µg/L cells ...
-
bioRxiv - Biochemistry 2022Quote: A polyclonal HEK cell line that stably expresses AE1 upon tetracycline induction was generated using the TRex system (Invitrogen). Cells were plated in a 96-well plate and incubated overnight with or without 2 μg/ml tetracycline at 37° C ...
-
bioRxiv - Cell Biology 2019Quote: ... C2C12 and Fyn-KD-HEK 293T) were carried out using Lipofectamine 3000 according to the manufacturer’s instruction (Life Technologies). Cells were checked for expression of plasmid of interest after 18 hours post transfection by fluorescence microscopy ...
-
bioRxiv - Microbiology 2020Quote: ... 1.5 × 105 HEK-293A cells were seeded in 12-wells plates and transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with 180 ng pcDNA3-DDX3-HA ...
-
bioRxiv - Immunology 2021Quote: HEK 293T-hACE2 and Vero E6 cells (RRID:CVCL_0574) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) containing 10% fetal bovine serum ...
-
bioRxiv - Cell Biology 2021Quote: Plasmids encoding full length SARS-CoV-2 S (GenBank ID: QHD43416.1) with one or more mutation sites were carried into HEK-293T cells using lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instruction ...
-
bioRxiv - Immunology 2020Quote: ... HEK 293T cells (2.5 × 105 cells/well in 24 well plates) were grown in DMEM (Gibco Cat# 11995-065) with 10% FBS and 1X PSA (100 units/mL penicillin ...
-
bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2019Quote: ... 70% confluent HEK-293T cells were cultured for 24 h w/o antibiotics and transfected (Lipofectamine, Thermo Fisher Scientific) with lentiviral transfection constructs and packaging plasmids (psPAX2 and pMD2.G ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and HEK 293T AAVS1 LP (HEK293T-LP, kind gift from D.M. Fowler) were cultured respectively in RPMI medium 1640 (Gibco) or DMEM medium (Gibco ...
-
bioRxiv - Immunology 2022Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
-
bioRxiv - Neuroscience 2022Quote: Human SH-SY5Y neural cells and HEK-293T cells were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
-
bioRxiv - Microbiology 2023Quote: ... 5×104 HEK 293T and 2.5×104 MDCK-SIAT1 cells were plated in 12 well plates in growth media (DMEM, Invitrogen #19965-092 ...
-
bioRxiv - Immunology 2023Quote: ... HEK 293T cells and HeLa cells were transfected with plasmids using lipofectamine 3000 according to the manufacturer’s instruction (Invitrogen).
-
bioRxiv - Immunology 2023Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Clonal genes were delivered as purified plasmid DNA ready for transient transfection in HEK Expi293 cells (Thermo Fisher Scientific). Cultures in a volume of 1.2 mL were grown to 4 days ...
-
bioRxiv - Bioengineering 2023Quote: All cell culture experiments were performed with HEK-293T cells (ATCC, CRL-3216) grown in 10% FBS / 90% Dulbecco’s Modified Eagle Medium (DMEM; Gibco) in a 37°C / 5% CO2 incubator with 100% relative humidity ...
-
bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
-
bioRxiv - Synthetic Biology 2023Quote: HEK 293T cells were lysed 16 hours post-transfection with 200 µL of M-PER lysis buffer (Thermo Scientific) plus protease inhibitors ...
-
bioRxiv - Microbiology 2023Quote: ... and HEK 293T-hACE2 (BEI Resources, NIAID, NIH, NR-52511) were cultured in Dulbecco’s Modified Eagle Media (DMEM; Gibco) with 10% (vol/vol ...