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Citations for Thermo Fisher :
1951 - 2000 of 7360 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Y-A or YIY-AAA) and FLAG-tagged CDK-1 constructs were expressed in FreeStyle 293-F cells (ThermoFisher Scientific). Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... were transfected with expression vectors for 3xFLAG-tagged wt eIF3g or its mutated forms using Expifectamine 293 according to the manufacturer’s protocol (Thermo Scientific). Cells expressing 3xFLAG-tagged eIF3g (200 ml suspension ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Immunology 2023Quote: ... plasmids encoding for various SCTs were co-transfected with a plasmid encoding for an ER-retained BirA-ligase into FreeStyle 293-F cells (Invitrogen) using the 293-free transfection reagent (Merck ...
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bioRxiv - Biochemistry 2023Quote: The WDR76 knockout cell line was derived from Flp-In™-293 Cell Line purchased from Invitrogen (Carlsbad, CA, USA). The knockout was achieved using RNA-guided CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas9 system (51) ...
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bioRxiv - Immunology 2023Quote: The cells used for expression of the rIgE clones construct were Freestyle 293-F cells (Thermofisher, cat no: K900001/510029). A 1 mL stock of 1*10^7 cells were rapidly thawed from −80°C in 37°C water bath and transferred to 19 mL prewarmed Freestyle™ 293 Expression Medium (Thermofisher ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells were cultured in serum-free FreeStyle 293 Expression media using 125 mL disposable culture flasks (Thermo Fisher Scientific). Cells were shaken constantly at 125 rpm at 37°C with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: Murine neuroblastoma Neuro-2a cells (#CCL-131; ATCC, Manassas, VA, USA) and Flp-In T-REx 293 cells (Thermo Fisher) were maintained in DMEM (D5796 ...
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bioRxiv - Neuroscience 2023Quote: ... 7 x 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM (# 41965062, Thermo Fisher Scientific) containing 10% fetal bovine serum (#16000-044 ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- suspension cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Biochemistry 2023Quote: Expression and purification of secreted SIDT1 and SIDT2 extracellular domain The SIDT1ECD and SIDT2ECD proteins were expressed using FreeStyle 293-F cells (Invitrogen). The cells were cultured in OPM-293 CD05 medium (OPM Biosciences co. ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... EXPI293 cells were diluted to 0.5×105 cells per millilitre 48 hours prior to transfection with FreeStyle 293 Expression Medium (ThermoFisher Scientific). Cells counts were adjusted to 1.5×106 cells/mL immediately prior to transfection ...
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bioRxiv - Biochemistry 2024Quote: ... Stable cell lines for expression were generated using the doxycycline-inducible Flp-In T-Rex 293 system (Thermo Fisher Scientific) according to the manufacturer’s guidelines.
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding both the heavy chain and light chain for each antibody were co-transfected into FreeStyle 293-F cells (Invitrogen) using polyethylenimine ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... The full-length spike proteins were stabilized by introducing 2 prolines at amino acid positions 986 and 987 (Spike 2P) and transiently transfected in FreeStyle 293-F cells using Turbo293 (SpeedBiosystems) or 293Fectin (ThermoFisher). The constructs contained an HRV3C-cleavage site followed by His and/or streptagII tags for purification purposes ...
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bioRxiv - Neuroscience 2024Quote: ... Flp-In T-REx 293 cells were grown in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS; Invitrogen), 100µg/mL zeocin (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) constructs were expressed by transient transfection of FreeStyle 293-F cells (Thermo Fisher) with polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... Adeno-X 293 cells (Takarabio Cat #632271) were transfected with linearized adenoviral vectors using Lipofectamine and Plus Reagent (Thermo Fisher). Cells were dislodged with gentle agitation upon exhibiting late cytopathic effect and were lysed with three freeze-thaw cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293, Invitrogen) and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the heavy chain and light chain of the antibody were transfected at an equimolar ratio into Expi293F cells using ExpiFectamine 293 transfection kit (Gibco) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... 293 cells (106 cells) expressing CD177 were treated by 0.2 unit PI-PLC (cat# P-6466, ThermoFisher Scientific, Waltham, Massachusetts) in 0.5 ml PBS for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Primary human vein endothelial cells (HUVECs) were cultured in human endothelial SFM medium (Invitrogen) containing 20% foetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher) and cultured in AIMV medium (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Genomics 2020Quote: For Hi-C experiments knockdown of CLAMP was validated using the Western Breeze kit (Invitrogen). Antibodies used for detection were a previously described custom rabbit antiCLAMP (1:1,000 ...
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bioRxiv - Genomics 2020Quote: The psep-1::his-58::eGFP transgene was generated using three-site Gateway cloning (Invitrogen) in the MosSCI compatible vector pCFJ150 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10 % heat-inactivated fetal bovine serum (HI FBS, Thermo Fisher Scientific, Loughborough, UK) and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin ...
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bioRxiv - Microbiology 2019Quote: ... A 1:150 dilution of anti-His antibody labelled with AF647 (Thermo Fisher, Waltham, MA) was then added to cells ...
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bioRxiv - Cell Biology 2019Quote: ... The His-mCherry-fusion proteins were purified using a Ni-NTA column (Thermo Fisher Scientific). The eluted proteins were dialyzed in 1 L dialyzed buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Molecular Biology 2019Quote: ... The Jpx E1-E3 isoform was cloned into pEF1/V5-His vector (Invitrogen Cat# V92020), which contains an EF-1α promoter for mammalian expression and a T7 promoter for in vitro transcription ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...
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Genomic approach for conservation and the sustainable management of endangered species of the AmazonbioRxiv - Genomics 2020Quote: ... One microliter of amplification was mixed with 8.5 μL Hi-Di deionized formamide (Applied Biosystems), and 0.5 μL GeneScan 500 LIZ (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved 14His-SUMO and His-Ulp1 were removed by affinity to Ni-NTA Agarose (Invitrogen). The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... under the control of an inducible pBAD promoter (pBAD/His A, Thermofisher, Catalog number 43001) (Supplementary Table 5) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were subcloned into the pAc5.1/V5-His A vector (Invitrogen, V4110-20) with a GFP tag sequence at its 3’ end terminus ...
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bioRxiv - Microbiology 2019Quote: ... with 30% L929 supernatant and 20% fetal bovine serum (HI-FBS, Thermo Scientific, Waltham, MA) for 48 hours at 37°C under 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: His-V5-uL4-CGN mRNAs were obtained using a mMESSAGE mMACHINE T7 kit (Invitrogen #AM1344) using linear DNA templates ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction pellets were dried and resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... the reaction pellets were dried and resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies).
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bioRxiv - Biochemistry 2021Quote: ... His-tagged RRs were purified from clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) or HisPur Cobalt resin (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The his-tag was cleaved using AcTEV protease according to manufacturer’s instructions (Invitrogen, Carlsbad, CA), and the his-tagged protease and noncleaved uSpike protein were removed by binding to Ni-NTA resin ...
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bioRxiv - Cell Biology 2019Quote: ... the above mutants are sub-cloned into pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020) with EcoRI and XhoI (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with Alexa Fluor 488–conjugated 6x-His Tag monoclonal antibody (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2019Quote: ... was added to multiplexed product with Hi-Di™ Formamide (Thermo Fisher Scientific, Waltham, MA) to aid in denaturing ...
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bioRxiv - Microbiology 2020Quote: ... All pBAD inducible plasmids generated in this work were derived from pBAD/His A (Invitrogen). pJNS12 is pBAD-gfp-linker-virB flanked by NcoI and HindIII restriction sites ...