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Citations for Thermo Fisher :
2151 - 2200 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... together with the helper plasmids pHelper 1.0 (Gag and Pol) and pHelper 2.0 (VSVG) were transiently cotransfected into HEK 293T cells using Lipofectamine 2000 (Invitrogen, USA) to package lentivirus particles ...
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bioRxiv - Neuroscience 2022Quote: HEK cells were transfected with several Mover constructs one day after plating using PEI transfection in Opti-MEM medium (Gibco). Per coverslip ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293T (HEK 293T) cells (ATCC CRL-3216) were cultured at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Neuroscience 2019Quote: ... Media for HEK cells stably expressing NaV1.1 or NaV1.6 also contained 400 μg/ml G418 (Fisher Scientific 10-131-035) to select for transfected cells ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA from HEK 293T cells (N = 2 biological replicates) or whole mouse brain (N = 6 biological replicates) was extracted using TRIzol (ThermoFisher) and treated with RNase-free DNase I (Promega) ...
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bioRxiv - Genomics 2020Quote: Live cell images of NIH3T3 cells (Fig. 1B) and HEK 293T cells (Fig. S1B) were taken and digitally recorded using FLoid Cell Imaging Station (ThermoFisher). In the experiments with ActD treatments (Fig ...
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bioRxiv - Bioengineering 2020Quote: ... 2013) and co-transfected with plasmids containing Cas9 effectors into A375 or HEK 293FT cells using Lipofectamine 2000 (ThermoFisher 11668019). The transfected cells were selected with 2 μg ml-1 puromycin for 72 h ...
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bioRxiv - Genomics 2020Quote: HEK 293T and HeLa cells were obtained from ATCC and maintained in DMEM supplemented with 10% FBS (ThermoFisher Scientific, 10082147) and antibiotics at 37°C in 5% CO2.
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney 293T cells (HEK 293T) (Pear et al., 1993) were maintained in Dulbecco’s modified Eagle medium (DMEM; ThermoFisher Scientific) supplemented with FBS ...
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bioRxiv - Immunology 2019Quote: ... Fabs and IgGs were produced by transient transfection in suspension 293F or adherent HEK 293T cells using Lipofecatamine 2000 (Invitrogen) or polyethylenamine (PEI) ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney (HEK) 293T cells were acquired from ATCC and cultured in DMEM media supplemented with 10% fetal bovine serum (FBS; Gibco) and 100 U/mL of penicillin and 100 μg/mL streptomycin (Gibco) ...
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bioRxiv - Zoology 2022Quote: HEK 293T cells were a gift from Kristin Dittenhaffer-Reed (Hope College, USA) and grown in DMEM (ThermoFisher Sci. #11965092) with 10% FBS (ThermoFisher Sci ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant containing lentivirus was generated by seeding 3.8 x 105 cells x ml-1 HEK 293FT cells (Invitrogen R700-07) in 10 cm tissue culture plates (10 ml per plate ...
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bioRxiv - Neuroscience 2024Quote: Chinese Hamster Ovary (CHO) cells were maintained similar to HEK cells with the following differences: Culture media was IMDM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: For expression analysis of truncated WDR47 constructs HEK cells were transfected with different HA tagged Wdr47 constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK-293T cells were transfected with shRNA plasmid and helper plasmids psPAX2 and pMD2.G using Lipofectamine 2000 (Invitrogen #11668027) in Opti-MEM (Invitrogen #22600-050 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2nd generation lentiviral packaging plasmids into HEK 293T cells at 60-80% confluency with 60uL Lipofectamine3000 (Thermo Fisher Scientific; L3000008) and 50uL P3000 reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Blue Native PAGE was performed using lysates prepared from growing HEK-293T cells according to the manufacturer’s protocol (Thermo Fisher). 1ξ107 cells were collected and lysed using 1x Native PAGE lysis buffer including protease inhibitor cocktail and 1% digitonin ...
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bioRxiv - Neuroscience 2023Quote: ... Media in 10 cm TC dishes with HEK 293T cells (at around 70% confluence) were replaced with Opti-MEM (Invitrogen). Then the SSF-MOR plasmid ...
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bioRxiv - Immunology 2023Quote: ... the signal was evaluated after 48 hours by detection of GFP expression in the HEK-293T-hACE2 cells using Attune NxT Acoustic Focusing Cytometer (ThermoFisher) or BD Symphony Flow Cytometry.
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bioRxiv - Genomics 2023Quote: The the HEKs and the HDFs were separated and homogenized in 500 µL of TRIzol reagent (Invitrogen, Carlsbad, CA, USA) using a microhomogenizer following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Human embryonic kidney (HEK) epithelial-like cell line HEK293T was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965-118; GIBCO™, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: pRT074 was lentivirally packaged in HEK 293T cells (ATCC Cat. No. CRL-3216) DMEM complete medium: DMEM (Gibco, 11965-092) supplemented with 10% FBS (VWR ...
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bioRxiv - Microbiology 2023Quote: Approximately 8·106 HEK-293T cells were seeded into T75 flasks previously coated with poly-D-lysine (Gibco™ A3890401). After 16 - 24 h (when cells reached around 90% confluence) ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T and HuTu-80 (CLS, 300218) cell lines were expanded in growth medium (DMEM or DMEM:F12, respectively; Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: The human embryonic kidney 293EBNA1-6E cell line (HEK 293EBNA1-6E) was adapted to growth in suspension in F17 medium (FreeStyleTM F17 Expression Medium, Gibco), supplemented with 4 mM L-Glutamine (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus-compatible expression vectors generated from Gateway Cloning were transfected in HEK-293T cells with Lipofectamine 3000 Transfection Reagent (cat. no. L3000001, Invitrogen) along with psPAX2 and pVSV-G viral packaging plasmids (Addgene ...
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bioRxiv - Immunology 2021Quote: ... and pFUSE2ss-CLIg-hk or pFUSEss-CLIg-hL2 respectively and were co-transfected in equal parts using ExpiFectamine™ 293 Transfection Kit(Gibco) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension HEK293S GnTI− cells expressing dolphin Prestin were grown at 37 °C and 7.8% CO2 in t FreeStyleTM 293 expression medium (Gibco Fisher Scientific) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The expression plasmids encoding HC and LC sequences were co-transfected into Expi293F cells using ExpiFectamine™ 293 Transfection reagents (Gibco). For IgG expression ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Neuroscience 2019Quote: ... 7 × 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM containing 10 % fetal bovine serum (ThermoFisher, USA) and penicillin/streptomycin ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293F cells were cultured at 37 °C with 6% CO2 and 70% humidity in Free Style 293 medium (Thermo Fisher Scientific) supplemented with 1% fetal bovine serum (FBS).
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bioRxiv - Immunology 2019Quote: ... the transfection mix was added to 1L cells at a density of ~1.2×106 cells/ml in FreeStyle 293 Expression Medium (Thermo Fisher - 12338018). The cells were incubated at 37°C and 8% CO2 for 6 days ...
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bioRxiv - Immunology 2021Quote: ... containing additional S383C and D985C substitutions (Henderson et al., 2020) with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Biochemistry 2021Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Biochemistry 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... Expi293F™ cells were grown in Expi293™ media and transiently transfected using ExpiFectamine™293 (all from Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 HexaPro Spike protein for cryoEM analysis was produced in HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m ...
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bioRxiv - Biophysics 2020Quote: Stable inducible Flp-In T-Rex-293 cell line expressing SERCA2a was generated using the Flp-In T-REx Core Kit (Invitrogen, USA) as described (28) ...
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bioRxiv - Microbiology 2022Quote: ... Cells grown to a density of 3 million cells per milliliter were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and grown for 4 days ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... These vectors were either transiently transfected or stably transfected into Flp-In™ 293 cells using Lipofectamine LTX (Thermo Fisher Scientific). 500 ng of DNA and 1 µL of Lipofectamine LTX or 10 µg of DNA and 44 µL of Lipofectamine LTX were used for transfection into each well of the 24-well plate (seeding density 2.0×105 cells/well ...
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bioRxiv - Immunology 2020Quote: ... Expi293F™ cells were grown in Expi293™ media and transiently transfected using ExpiFectamine™293 (all from Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... 293 cells from a pMLINK tetracistronic vector (courtesy of Y. Shi, Tsinghua University, Beijing).45 HEK293 cells were cultured in Freestyle 293 media (Life Technologies), shaking at 125 rpm while incubating at 37 oC with 8% CO2 until a density 2 × 106 cells/ml was reached ...
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bioRxiv - Biochemistry 2021Quote: ... MERS-CoV S2-apex-less (MERS-CoV S2-only construct with residues 811–824 replaced with GGSGGS and residues 1042–1073 replaced with a flexible linker) were expressed in Freestyle 293-F cells (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for 3-5 days ...
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bioRxiv - Immunology 2020Quote: ... spike protein was expressed by transiently transfecting plasmid encoding the HexaPro spike variant2 containing substitutions S383C and D985C3 with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Immunology 2020Quote: ... and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific). After 7 days of culture at 37°C and 5% CO2 ...