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Citations for Thermo Fisher :
1951 - 2000 of 5010 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... cDNA was produced from the extracted RNA using Superscript II enzyme from Invitrogen. RT-qPCR was performed on obtained cDNA using the iQ™ SYBR® Green supermix (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained for 20 min with SYBR™ Green II (Invitrogen™) and imaged using a Bio-Rad ChemiDoc imaging system.
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bioRxiv - Genomics 2023Quote: ... nuclei were counted using trypan blue and an automated hemocytometer (Countess II; Invitrogen) and diluted to a concentration of 1000 cells/µl.
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bioRxiv - Microbiology 2023Quote: ... cDNA first strand was synthesized with SuperScript-II Reverse Transcriptase (Thermo Fisher Scientific) for 10 min at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
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bioRxiv - Neuroscience 2023Quote: ... 100ng of total RNA were used for reverse transcription using SuperScript II (Invitrogen). mRNA levels were assessed by qPCR (Biorad ...
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bioRxiv - Biochemistry 2024Quote: ... using XCell SureLock Mini-Cell system with the XCell II Blot Module (Invitrogen). The membrane was cross-linked 5 min with UV ...
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bioRxiv - Microbiology 2024Quote: ... and 2mM copper (II) sulfate pentahydrate all dissolved in UltraPure distilled water (Invitrogen) (90) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were counted using the Countess II FL Automated Cell Counter (Thermo Fisher). Cell suspensions were adjusted to a concentration of 800-1200 cells per microliter (8e5 - 1.2e6 cells per mL ...
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bioRxiv - Genomics 2024Quote: ... Reverse transcription was conducted with SuperScript II Reverse Transcriptase (Life Technologies, 18064-071) using a barcode-specific primer (P7-pLSmP-ass16UMI-gfp ...
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bioRxiv - Cell Biology 2024Quote: ... were transfected with the bacmid DNAs using Cellfectin II (10362100; Thermo Fisher Scientific).
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bioRxiv - Pathology 2024Quote: ... 1.5 µg of total RNA was reverse-transcribed using Superscript II (Life Technologies). Relative expression levels of genes of interest were determined by real-time RT-PCR using the Absolute SYBR Green ROX Mix (ABgene ...
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bioRxiv - Genetics 2024Quote: ... which were converted into cDNA with Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) according to manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA synthesis was performed with 1ug RNA and SuperScript II reverse transcriptase (Invitrogen) following manufacturer’s instructions and was diluted 1:20 in water before qPCR analysis ...
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bioRxiv - Cell Biology 2024Quote: ... and the destination vector pDestTol2CG2 were recombined using LR Clonase II Plus (Invitrogen). To make the pDestTol2CmC2 TRE:GSP-il1βmat construct ...
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bioRxiv - Biophysics 2024Quote: ... SF9 insect cells were cultured in Sf-900™ II SFM medium (Gibco) supplemented with 1 x GlutaMax (Gibco ...
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bioRxiv - Genetics 2024Quote: AC16 cells were grown on Nunc Lab-Tek II Chamber slides (ThermoFisher, 154461). Cells were fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Immunology 2024Quote: ... reverse transcription was done with SuperScript™ II reverse transcriptase (Invitrogen, Catalog: 18064014), oligo-dT30VN (1 µM ...
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bioRxiv - Neuroscience 2024Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). A Sequant ZIC-pHILIC column (2.1 mm i.d ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was changed to Stage II media: Neurobasal-A media (Gibco, 10888022) with 1% penicillin-streptomycin ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was reverse-transcribed using the SuperScript II Reverse Transcriptase Kit (Invitrogen, USA), following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... and transfected into adherent Sf9 insect cells using Cellfectin II (Invitrogen 10362-100). After 7 days ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell counts were performed with a countess II automated cell counter (Thermo Fisher).
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bioRxiv - Biochemistry 2024Quote: The TC-FlAsH II In-Cell Tetracysteine Tag Detection Kit (Invitrogen catalog #T34561) was used to obtain FlAsH-EDT2 and dimercaprol/British anti-Lewisite (BAL ...
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bioRxiv - Systems Biology 2024Quote: ... mRNA samples were reverse-transcribed using Superscript II Reverse Transcriptase (Thermo Fisher Scientific) and cDNAs were amplified using the Smart-seq2 protocol (Picelli et al. ...
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bioRxiv - Cell Biology 2024Quote: ... followed by counting in chamber slide using Countess II Automated Cell Counter (Invitrogen). Resuspension buffer was added to the nuclei suspension to achieve a final concentration of 700-1200 nuclei/µl ...
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bioRxiv - Systems Biology 2024Quote: ... RNAs were then subjected to reverse transcription using SuperScript II (Life Technologies, # 18064014) or high-capacity cDNA reverse transcription kits (Applied Biosystems ...
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bioRxiv - Systems Biology 2024Quote: ... with 4mg/mL AlbuMAX™ II Lipid-Rich Bovine Serum Albumin (GIBCO™), 1× MACS NeuroBrew-21 with Vitamin A (Miltenyi Biotec) ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse transcription was performed with 500 ng of RNA using superscript II (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... hRPE1 cell were seeded in Lab-TeK II 8-chambered coverglass (Nunc, Rochester, NY) and transfected with GFP-2xHrs or GFP-WIPI2 ...
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bioRxiv - Immunology 2021Quote: ... The RT master mix per well consisted of: 0.2μL SuperScript II 5x buffer (Invitrogen), 0.03μL SuperScript II taq (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... First strand cDNA was made using the Superscript II RT-PCR (Invitrogen, 11904-018) protocol with 100 ng of total RNA for conversion ...
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bioRxiv - Microbiology 2021Quote: ... destination vector using Gateway™ LR Clonase™ II Enzyme Mix (Cat. # 11791020, Invitrogen). pEZY-FLAG-Nsp14 vector was constructed from pDONR223 SARS-CoV-2 Nsp14 vector to the pEZY-FLAG (Cat # 18700 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell count for calculating the growth rate was performed by Countess II (Thermo Fisher).
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bioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription with the input material of 2ng was performed using SuperScrpit II (Invitrogen) in the presence of oligo-dT30VN (1μM ...
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bioRxiv - Developmental Biology 2020Quote: ... We then performed first strand cDNA synthesis using SuperScript II Reverse Transcriptase (Thermo Fisher). We amplified the relevant cDNAs using Phusion polymerase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Cell viability was assessed with Trypan blue exclusion on a Countess II (ThermoFisher Scientific) automated counter and showed a >85% viability ...
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bioRxiv - Neuroscience 2021Quote: ... and converted to cDNA using oligo-dT primers and Superscript II reverse transcriptase (Invitrogen). Quantitative RT– PCR was performed using Power SYBR Green PCR Master Mix (ABI ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell density and viability were measured on a Countess II Automated Cell Counter (Invitrogen). Upon culture termination ...
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bioRxiv - Developmental Biology 2022Quote: ... Total ribonucleic acid (RNA) from 250,000 cells was reverse transcribed by Superscript II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... and as a part of quality control using Platinum SuperFi II DNA Polymerase (Invitrogen). A forward primer Mt2120F (5’-GGA CAC TAG GAA AAA ACC TTG TAG AGA GAG −3’ ...
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bioRxiv - Immunology 2022Quote: ... and cell viability was reassessed using a Countess II Automated Cell Counter (ThermoFisher Scientific). Viabilities from the Countess readings were deemed adequate to proceed with partitioning (>76% live cells per sample ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were counted using a Countess™ II FL (Thermo Fisher Scientific, Durham, NC). All samples exhibited 100% cell death with DAPI staining ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded in Nunc Lab-Tek II Chamber slides (Life Technologies, Carlsbad, USA) and processed for treatment ...
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bioRxiv - Neuroscience 2019Quote: ... Then we performed an LR reaction (GatewayTM LR ClonaseTM II Enzyme mix, Thermo Scientific) to insert the Hr38 enhancer into pZT2.0 to make Hr38-Tomato.
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA was extracted using Phire Animal Tissue Direct kit II (ThermoFisher Scientific, F140WH) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 3µg of mRNA was then reverse transcribed using Superscript II using oligodT primers (Invitrogen). Reverse transcriptase (RT)-PCR was performed at 42°C for 50 min ...
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bioRxiv - Physiology 2019Quote: ... cDNA synthesis (mRNA) was performed using 500ng RNA and SuperScript II (Invitrogen, Paisley UK) and cDNA synthesis (microRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were cloned into the pCR-Blunt II-TOPO vector (Thermo Fisher Scientific) and subcloned into the pLV-EF1a-IRES-Puro vector (Addgene plasmid #85132 ...
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bioRxiv - Developmental Biology 2019Quote: ... The construct was ligated into a pCR Blunt II Topo vector (Thermo Fisher Scientific). The Cdh2-HA insert was then ligated into a p2Lox-eGFP plasmid ...