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Citations for Thermo Fisher :
2101 - 2150 of 5010 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: All cells were grown and transfected on Lab-Tek II chambered #1.5 coverglasses (ThermoFisher) or MatTek dishes with #1.5 coverslip (MatTek Corporation ...
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bioRxiv - Cell Biology 2019Quote: Anti-Ii for live immunostaining was labelled using Monoclonal antibody labeling kit (Molecular Probes) according to manufacturer’s protocol with AlexaFluor™-488 ...
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bioRxiv - Cell Biology 2019Quote: ... Bacmid was purified and transfected into Sf9 cells using Cellfectin II reagent (Thermo Fisher). High-expressing clones were identified and baculovirus was generated for two additional passages ...
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bioRxiv - Genomics 2021Quote: ... Then it was incubated in 5 mg/ml Dispase II (Thermo Fisher Scientific, 17105041) in HBSS (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... coupled to the EASY nLC II high performance liquid chromatography system (Proxeon, Thermo Scientific). Peptide separation was performed on a reverse phase C18 column (Nikkyo Technos) ...
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bioRxiv - Physiology 2021Quote: ... First-strand cDNA was transcribed using random primers and SuperScript II Reverse Transcriptase (Invitrogen). Real-time quantitative PCR was performed using ABsolute SYBR Green ROX mix (Thermo Scientific) ...
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bioRxiv - Physiology 2021Quote: ... and first-strand cDNA synthesis performed (0.5 µg total RNA) with SuperScript II (Invitrogen). PCR was performed using Eppendorf Realplex2 instrument with the gene specific primers as indicated (Supplementary Table 2) ...
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bioRxiv - Physiology 2021Quote: ... minced mechanically and digested with 0.2% collagenase II (Invitrogen, cat. 17101015, Carlsbad, CA, USA) and 0.05% trypsin in PBS for 40 min at 37 °C with slight agitation ...
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bioRxiv - Physiology 2020Quote: ... and first-strand cDNA synthesis performed (2 µg total RNA) with SuperScript II (Invitrogen). PCR was performed using Eppendorf Realplex2 instrument with the gene specific primers (IDT ...
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bioRxiv - Microbiology 2020Quote: ... siRNA (Table S1) was transfected into C6/36 cells with Cellfectin II reagent (Invitrogen). JEV was inoculated at 24 h post transfection ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from 1 µg of RNA using SuperScript II reverse transcriptase (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... Purified RNA was then subject to reverse transcription with SuperScript II Reverse Transcriptase (Invitrogen) and quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2021Quote: ... which were maintained in SFM II 900 medium (Thermo Fisher Scientific, Waltham, MA, USA) at 27°C and 120 rpm ...
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bioRxiv - Biochemistry 2021Quote: Baculovirus were generated by transfecting Sf9 cells using Cellfectin II reagent (Thermo Fisher Scientific), according to the standard Bac-to-Bac protocol or linear polyethylenimidine (PEI MAX ...
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bioRxiv - Cell Biology 2021Quote: ... After cDNA synthesis using SuperScript® II Reverse Transcriptase kit (Invitrogen, Carlsbad, CA, USA) and random hexamers ...
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bioRxiv - Plant Biology 2021Quote: ... Entry clones were recombined into pDEST22 destination vector with the LR-II clonase (Invitrogen). Small scale Y2H assays was performed by co-transforming bait and pray plasmids as described in (De Folter and Immink ...
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bioRxiv - Cancer Biology 2021Quote: ... random primed and reverse transcribed using SuperScript II Reverse Transcriptase (Invitrogen, part # 18064-014) with the addition of Actinomycin D ...
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bioRxiv - Pathology 2021Quote: ... Total ribonucleic acid (RNA) from 250,000 cells was reverse transcribed by Superscript II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... controlled through the use of a tri-gas incubator (ThermoFisher Forma Series II 3130), at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... when an additional volume of 1.6 U/ml of dispase II (Life Technologies, #17105041) was added creating a final concentration of 0.8 U/ml dispase II ...
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bioRxiv - Developmental Biology 2021Quote: ... Counting was performed using the Countess™ II Automated Cell Counter (Applied Biosystems; A27977).
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bioRxiv - Cell Biology 2020Quote: ... using the Gateway LR clonase II Enzyme mix (Thermo Fisher Scientific, cat# 11791-020).
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bioRxiv - Genetics 2021Quote: ... and 2 U of Phusion Hot Start II DNA Polymerase (Thermo Fisher Scientific, F549S) in 1X HF buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Vector recombination was performed using the Gateway LR Clonase II enzyme kit (Thermo Fisher).
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bioRxiv - Immunology 2020Quote: ... cells were seeded on Chamber Slides® (Lab Tek II, 8 well, Thermo Fisher). To monitor acidification of bacteria-containing compartments ...
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bioRxiv - Microbiology 2020Quote: ... and 0.02 U/µl of Phusion® Hot Start II Taq Polymerase (Thermo Scientific). PCR profile included an initial denaturation of 30 s at 98 °C ...
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bioRxiv - Immunology 2021Quote: ... Purified RNA was reverse-transcribed using Superscript II enzyme and random hexamer primers (Invitrogen). Taqman probes for genes of interest were purchased from Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... using the primers shown in Table II and cloned into pcDNA-V5/His (Invitrogen). pCAGGS-EboGP-V5 (strain Zaire 1976 Mayinga ...
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bioRxiv - Immunology 2020Quote: ... Complementary DNA was generated from 1LJμg of total RNA with Superscript II RT (Invitrogen) and random hexamer primers (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... and High Five cells were maintained in suspension in Sf-900 II SFM (Gibco).
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bioRxiv - Immunology 2020Quote: ... at 40.000 cells/well or in 4-well Lab-Tek II chambered coverglass (Nunc) at 100.000 cells/chamber ...
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bioRxiv - Immunology 2021Quote: ... and cells were counted using an automated cell counter Countess II (Thermo Fisher Scientific). Viability of > 60% was maintained after the overnight rest ...
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bioRxiv - Cell Biology 2021Quote: ... 500ng of total RNA was used to synthesize cDNA with SuperScript II (ThermoFisher Scientific) using Random primers (Promega ...
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bioRxiv - Genetics 2020Quote: ... 5mM dithiothreitol and 200 U SuperScript® II Reverse Transcriptase (ThermoFisher Scientific, Illkirch, France). The reaction was incubated at 42°C for 50 min and inactivated by heating at 70°C for 15 min ...
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bioRxiv - Genomics 2021Quote: ... Nuclei concentration was determined using the Countess II FL Automated Cell Counter (ThermoFisher; AMQAF1000).
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bioRxiv - Cancer Biology 2021Quote: ... A homologous recombination reaction was then performed using LR Clonase II (Life Technologies #11791100) with the pDONR223-ZFP36L1- WT or pDONR223-ZFP36L1-MUT plasmids and DOX-On pTripZ-NEO DEST or pLX304-CMV DEST vectors that were both modified to be used as destination vectors for gateway recombination cloning ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were counted using the automated cell counter Countess II (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2022Quote: 3617 and 3T3-L1 cells were plated in LabTek II (ThermoFisher, Waltham, MA, USA) or Cellvis (Mountain View ...
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bioRxiv - Cell Biology 2022Quote: ... coupled to a Proxeon EASY-nLC II liquid chromatography (LC) pump (Thermo Fisher Scientific). Peptides were separated on a 75μm (inner diameter ...
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bioRxiv - Cell Biology 2022Quote: ... which was then transformed into Sf9 cells using Cellfectin II reagent (Invitrogen; cat#10362100) to produce virus stock ...
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bioRxiv - Genetics 2022Quote: ... pmE-zfCre and p3E-MCS1 in a multisite reaction using LR Clonase II (Thermofisher). For transgenic expression of CreERT2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These samples were loaded onto an OnGuard column (Dionex OnGuard II Cartridge) (Thermo Scientific) to remove the sulphate and halogen ...
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bioRxiv - Physiology 2022Quote: ... and reverse transcribed using 1,000 ng of RNA and Superscript II enzyme (18064014, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... operated at 300 kV acceleration voltage with a Falcon II direct electron detector (ThermoFisher) or were acquired on a Glacios electron microscope (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... the transfected cells were supplemented with Protein-Free Hybridoma Medium Supernatant (PFHM-II, Gibco). The supernatant containing secreted mAb was harvested at day 4 and purified using protein A-agarose beads (Thermo Fisher ...
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bioRxiv - Pathology 2022Quote: ... and subsequent RT-PCR was conducted using the Superscript II Reverse Transcriptase kit (Invitrogen). Samples were incubated at 42°C for 50 min followed by incubation at 70°C for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... Cell densities were measured using Countess II FL Automated Cell Counter (Thermo Fisher Scientific). Single mutants on CP29 (k9 ...
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bioRxiv - Plant Biology 2022Quote: ... For all genotyping amplification reactions Phire Hot Start II DNA Polymerase (Thermo Fisher Scientific) was applied.
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized from the fragmented RNA using SuperScript II Reverse Transcriptase (Invitrogen, 18064014) and random primers ...
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bioRxiv - Physiology 2023Quote: ... RNA was treated with DNase and reverse-transcribed using Superscript II reverse transcriptase (Invitrogen). The resulting cDNA was used for real-time RT-PCR on a QuantStudio 5 Real-Time PCR system using RealQ Plus 2x Master Mix Green (Ampliqon ...