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Citations for Thermo Fisher :
1751 - 1800 of 4764 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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miRNA activity contributes to accurate RNA splicing in C. elegans intestine and body muscle tissues.bioRxiv - Molecular Biology 2019Quote: ... we performed several Gateway LR Clonase II plus reactions (Invitrogen, cat. 12538-013) using the destination vector CFJ150 (Frokjaer-Jensen et al ...
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bioRxiv - Bioengineering 2019Quote: ... Copper (II) sulfate pentahydrate (CuSO4·5H2O) was purchased from Acros Organics (Geel, Belgium). Alkyne-functionalized PLP with an N-terminal 4-pentynoic acid (homopropargyl ...
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bioRxiv - Microbiology 2019Quote: ... Each reaction contained 12 µL of AccuPrime™ SuperMix II (Thermo Scientific™), 0.5 µL of forward and reverse primer from a 10 µM stock ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed to cDNA by random hexamers and SuperScript II (Invitrogen). Quantitative real-time PCR was performed following the manufacturer’s protocol by using Power SYBR Green PCR Mix (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: Cell enumeration using the Countess™ II Automated Cell Counter (AMQAX1000, Thermo Fisher) was used for routine cell density and viability estimations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reverse transcription was performed using random hexamer primers (Fermentas) and SuperScript II (Invitrogen), with annealing ...
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using Superscript II™ Reverse Transcriptase (Invitrogen, Life Technologies Ltd.) with sample randomisation ...
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bioRxiv - Systems Biology 2020Quote: ... Junction PCR was performed using Phire Hot Start II DNA Polymerase (Thermo Scientific), and the PCR amplicons were subjected to Sanger sequencing for identification of clones that contained the expected junction sequences at both left and right homology junctions.
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bioRxiv - Cell Biology 2020Quote: ... and used to transfect Sf9 cells using Cellfectin II transfection reagent (Thermo Fisher), and baculovirus was amplified as previously described (Llauró et al. ...
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using Superscript II™ Reverse Transcriptase (Invitrogen, Life Technologies Ltd.) with sample randomisation ...
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bioRxiv - Biochemistry 2021Quote: ... purified bacmid DNA was transfected into Sf9 cells using Cellfectin II (ThermoFisher Scientific). After two rounds of amplification ...
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bioRxiv - Microbiology 2021Quote: ... The inserts were amplified by PCR using a Platinum SuperFi II mastermix (ThermoFisher) and assembled into full length SARS-CoV-2 cDNA clones in the YAC vector pYESL1 using a GeneArt™ High-Order Genetic Assembly System (A13285 ...
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bioRxiv - Neuroscience 2021Quote: ... cells and transfected into Sf9 insect cells using Cellfectin II reagent (ThermoFisher, 10362100). Baculovirus was generated according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Complementary DNA was obtained by reverse transcription (Superscript II, catalog no. 18064014, Invitrogen) of 2 μg RNA with an equimolar mixture of 3’-anchored poly-T oligonucleotides (T18V ...
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bioRxiv - Plant Biology 2021Quote: ... were recombined into pDONR221 vector with Gateway BP clonase II enzyme mix (Invitrogen) to generate the corresponding entry clones ...
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bioRxiv - Biophysics 2021Quote: ... and 5% CO2 in a ThermoForma Series II Water-Jacketed CO2 Incubator (ThermoFisher). Main HEK-293T stocks were grown in T-25 Flasks (Corning ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell numbers and viability were determined using Countess II (Thermo Fisher Scientific, AMQAX1000).
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bioRxiv - Cancer Biology 2020Quote: ... or counting by Trypan exclusion on an automated cell counter (Countess II, ThermoFisher). Luminescence was measured using a microplate reader (Biotek Synergy HTX).
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bioRxiv - Cell Biology 2020Quote: ... and pTcINDEX-GW vectors by recombination using LR clonase II enzyme mix (Invitrogen). The pDEST17 constructs were transformed into Escherichia coli BL21 pLysS and recombinant proteins ...
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bioRxiv - Cell Biology 2020Quote: ... they were plated on a LabTek II chambered coverglass (#155409, Thermo Fisher Scientific) and imaging was performed using the same Spinning-Disk microscope as for embryos ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR products were transferred into pDONR223 in a BP Clonase II (Invitrogen)-mediated recombination reaction ...
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bioRxiv - Microbiology 2020Quote: RNA was reverse transcribed into cDNA using SuperScript II Reverse Transcriptase (Thermo Fisher) with oligo d(T ...
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Bioaugmented sand filter columns provide stable removal of pesticide residue from membrane retentatebioRxiv - Microbiology 2020Quote: ... the PCR mixture contained 12 µl AccuPrime Supermix II (Invitrogen, Eugene, Oregon, US), 0.25 µM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... 5 - 10 μg of total RNA in Gel Loading Buffer II (GLII, Ambion) was loaded per lane on 6% polyacrylamide (PAA ...
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bioRxiv - Immunology 2021Quote: ... lymph nodes were enzymatically digested with 100U/mL of collagenase type II (Gibco) at 37°C for 30 min to liberate lymphocytes and antigen-presenting cell populations ...
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bioRxiv - Molecular Biology 2021Quote: ... First strand synthesis was performed with Random Primer and Superscript II RT (Invitrogen) at 25°C for 10 min ...
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bioRxiv - Immunology 2020Quote: ... or was completed during one-step qPCR using SuperScript II Reverse Transcriptase (Invitrogen). Real-time PCR was performed with the GoTaq qPCR Mastermix (Promega ...
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bioRxiv - Molecular Biology 2020Quote: RNA (20-200 ng) was reverse transcribed with Superscript II Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions using gene-specific reverse (R ...
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bioRxiv - Molecular Biology 2020Quote: 10 µg total RNA samples were mixed with gel loading buffer II (ThermoFisher) and separated by 10% or 15% TBE-urea gels (Novex ...
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bioRxiv - Cell Biology 2021Quote: ... The two sequences were cloned separately into pCR Blunt II-Topo vectors (Invitrogen). For the GalT-pHlemon plasmid ...
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bioRxiv - Immunology 2021Quote: ... Mtb genomes were then quantified using Taqman Universal Master Mix II (Life Technologies) and previously published sigF primer-probe combination (Lin et al. ...
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bioRxiv - Immunology 2022Quote: ... Cell number was determined using a Countess II FL automated cell counter (Invitrogen). Adhesion profile was assessed by two independent observers to determine the total number of adhesions within the peritoneal cavity after surgery and also an arbitrary adhesion score ...
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bioRxiv - Genetics 2022Quote: ... Cells were counted with Trypan blue using a Countess II automated counter (ThermoFisher) and then passaged every 2-3 days ...
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bioRxiv - Plant Biology 2022Quote: ... One microgram of total RNA was used for reverse transcription (Superscript II, Invitrogen). Transcript levels were quantified by quantitative PCR in a Stratagene MX3005P instrument (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... reagents used for the reverse transcription (RT) reaction such as Superscript II (Invitrogen) and RNasein Plus (Promega) ...
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bioRxiv - Bioengineering 2022Quote: ... and 10 nM (1x) of SYBR™ Green II RNA Gel Stain (Invitrogen) in the reaction buffer -containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Bioengineering 2022Quote: ... and 10 nM (1x) of SYBR™ Green II RNA Gel Stain (Invitrogen) in the reaction buffer containing 40 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... For cell count we used Countess II automated cell counter (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2022Quote: ... following this cells were counted using an automated cell counter (Countess II, Invitrogen). If cells were to be treated with para-Amino-Blebbistatin (Cayman Chemical ...
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bioRxiv - Physiology 2022Quote: ... cDNA was generated using random primers and Superscript II reverse transcriptase (Life Technologies). Quantitative real-time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using the SuperScript™ II Reverse Transcriptase (Invitrogen) and qPCRs using the qPCRBIO SyGreen Mix Separate-ROX (NIPPON).
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bioRxiv - Microbiology 2022Quote: ... biofilms were grown in Nunc™ LabTekTm II Chamber Slide™ System (Thermofisher) with 1 mL of diluted culture ...
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bioRxiv - Microbiology 2022Quote: ... RNA was reverse-transcribed using SuperScript® II RT (Invitrogen, Carlsbad, CA, USA), oligo dT and random primers ...
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bioRxiv - Plant Biology 2022Quote: ... The reaction was catalyzed by LR Clonase™ II Mix (Thermo Fisher Scientific). Finally ...
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bioRxiv - Physiology 2022Quote: ... C-IV-II and C-V-alpha (ThermoFisher Scientific, 45-8199; 1:2500). Secondary antibodies used were horseradish peroxidase–conjugated anti-mouse (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). A Sequant ZIC-pHILIC column (2.1 mm i.d ...
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bioRxiv - Plant Biology 2022Quote: ... and cloned into the pDONR207 using a BP Clonase II Kit (Thermo Fisher). Chemically competent Escherichia coli DH5α cells were transformed by the heat-shock method (Dagert and Ehrlich 1979) ...
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bioRxiv - Plant Biology 2022Quote: ... Using the Gateway® LR Clonase™ II kit (Invitrogen Corp., Carlsbad, CA), DDP1 was recombined with Gateway plant destination vectors pK7FWG2 (C- terminus DDP1-GFP ...
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bioRxiv - Neuroscience 2022Quote: ... (ii) Incubation with a buffer containing hydroxylamine (NH2OH, Fisher Scientific, Cat no. 26103) that cleaves cysteine-linked palmitate thioester bonds or with control buffer that instead of hydroxylamine contains Tris ...
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bioRxiv - Molecular Biology 2022Quote: Skin and wounds were incubated in dispase II (5 U/mL, ThermoFisher Scientific) at 4°C overnight ...