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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... 7% CO2 in a humidified incubator at 37°C and passaged by dissociation with Accutase (Thermo Fisher Scientific, A1110501) every 3-5 days ...
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bioRxiv - Biochemistry 2019Quote: ... 0.25 M cell equivalents were run on each lane of a 7% NuPage Tris-Acetate gel (Invitrogen, Carlsbad, CA) and then transferred to an Immobilon-FL PVDF membrane (EMD Millipore ...
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bioRxiv - Physiology 2020Quote: ... Quantitative real-time RT-PCR was performed on a ViiA™ 7 Real-Time PCR System (Applied Biosystems Inc.) The PCR program was ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection of cultured neurons was performed with 300 µl Opti-MEM medium mixed with 7 µl Lipofectamine 2000 (Invitrogen), 1 µl Magnetofection CombiMag (OZ Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Monocytes adhered to glass coverslips in 12-well plates (1.5×106 cells/well) and were subsequently cultured for 7–9 days in RPMI (Gibco) with 10% FBS (Wisent ...
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bioRxiv - Microbiology 2020Quote: ... swabs were directly incubated with 7 mL of Brain Heart Infusion (BHI) broth (Thermo Scientific Microbiology, Cat. No. CM1135B) at 37°C till turbidity was observed (14-16 h for >95% of samples ...
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bioRxiv - Neuroscience 2020Quote: ... the brains were cut into 7-μm thick coronal sections on a microtome (Microm HM 340 E, Thermo Scientific), and mounted on SuperFrost™ Plus glass slides (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Brains were embedded in paraffin and 7 µm-thick sections were cut on a paraffin microtome (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... The number of spheroids was assessed 7 days after plating through an EVOS Cell Imaging System (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... the proteins were subjected to a 7 KDa molecular weight cut-off Zeba Spin Desalting Column (Thermo Fisher Scientific), which was pre-equilibrated with 20 mM HEPES pH7.5 and 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and COS-7 cells were grown in DMEM and RPE1 cells in DMEM/F12 medium (Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2020Quote: ... Data were collected and analyzed using the QuantStudio™7 Flex Software (Applied Biosystems, Thermo Fisher Scientific, MA, USA). Differential gene expression was quantified based on the △ △Ct method using normalized geo-metric means of the two reference genes (Zm00001d002944 ...
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bioRxiv - Plant Biology 2020Quote: ... Data were collected and analyzed using the QuantStudio™7 Flex Software (Applied Biosystems, Thermo Fisher Scientific, MA, USA). Differential gene expression was quantified based on the △ △Ct method using normalized geo-metric means of the two reference genes (Zm00001d002944 ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... seeded with 2,000 cells/well and stained with Hoescht 33258 (1µg/mL) and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at a dilution of 1000x ...
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bioRxiv - Plant Biology 2021Quote: ... 100 g were transferred into a previously sterilized Magenta GA-7 plant culture box (Thermo Fisher Scientific, Schwerte, Germany), sealed and autoclaved again ...
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bioRxiv - Physiology 2020Quote: ... MCF-10 human mammary epithelial cells and COS-7 fibroblast-like monkey cells were maintained in DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 2% penicillin/streptomycin.
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bioRxiv - Immunology 2021Quote: ... biotinylated with 7:1 biotin-to-antibody using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (21435, Thermo Fisher Scientific), was prepared in dilution buffer ...
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bioRxiv - Physiology 2021Quote: ... qPCR was performed on 10ng of cDNA using the SYBR-green method on a QuantStudio 7 Flex (ThermoFisher Scientific) using gene specific primers ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was then applied to a Zeba Spin Desalting Column (7 kDa cut-off, Thermo Fisher Scientific, France) equilibrated in acquisition buffer containing 5% of the detergent concentration used for solubilization and centrifuged 2 min at 1,500 x g and 4°C ...
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bioRxiv - Biophysics 2020Quote: ... and 0.1 mM AMP-PNP) using 0.5-mL Zeba™ spin desalting column (7-kDa MWCO) (Thermo Scientific #89882). AMP-PNP and taxol were added to the flow-through to a final concentration of 1 mM and 10 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... in combination with TaqMan Gene Expression Assays per manufacturer protocol on a ViiA 7 RT-PCR system (Applied Biosystems). The assay IDs for TaqMan Gene Expression Assays used were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were run in a Quant Studio 7 Flex Thermal Cycler (Applied Biosystems, Life Technologies, Eugene, OR, USA). The primer efficiencies were ≥ 95% for all primer pairs ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were run in a Quant Studio 7 Flex Thermal Cycler (Applied Biosystems, Life Technologies, Eugene, OR, USA). The primer efficiencies were ≥ 95% for all primer pairs ...
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bioRxiv - Cell Biology 2022Quote: ... neurons (5-7 DIV) were transfected with 0.35–1.5 μg of total plasmid DNA using Lipofectamine 2000 Transfection Reagent (ThermoFisher, 11668030) and incubated for 18-24 h.
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bioRxiv - Bioengineering 2022Quote: COS-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, high glucose, GlutaMAX Supplement, pyruvate, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Huh-7 (Nakabayashi et al., 1982) and Huh-7.5 (Blight et al., 2002) were cultured in DMEM (Gibco 11965118) supplemented with 10% FBS and non-essential amino acid solution ...
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bioRxiv - Immunology 2022Quote: ... MCF-7 (ATCC HTB-22) breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, 11960-051) containing 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific, # 89960). Ligand binding affinity and sensitivity were assessed using ELISA techniques.
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were mounted to a glass slide with 7 µl Fluoromount-G (Invitrogen, 00-4958-02). They were sealed with nail polish within 10 minutes after mounting ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking buffer was replaced after one hour with 7 µg/mL mouse anti-occludin antibody (cat# 33-1500, Invitrogen) in PBS with 5% goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher, Foster City, CA, USA) using GoTaq qPCR master mix (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher, Foster City, CA, USA) using GoTaq qPCR master mix (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed with PBS and re-suspended in 0,2 % BSA in Dulbecco’s PBS with Sytox Blue or 7-AAD (LifeTechnologies, Thermo Fisher) to exclude dead cells ...
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bioRxiv - Bioengineering 2020Quote: ... gondii GT1 T NCBI Genome assembly version 7/19/2013 genome references with ERCC sequences (obtained from ThermoFisher website). The raw fastq files from sequencing are aligned to the concatenated genomes with STAR aligner (version 2.6.0c ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEAA and insulin as described above for MCF-7 cells and phenol-red free RPMI (Thermo Fisher Scientific), 10% charcoal-stripped fetal bovine serum ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were obtained by pushing the tissue through a 70 µm cell strainer and subsequently cultured at 7 × 106 cells/ml in stimulation medium consisting of RPMI1640 supplemented with 0.5% penicillin/streptomycin (Gibco), 10% FCS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... HuH-7 cell line was obtained from JCRB Cell Bank and cultured in DMEM (Gibco, catalog no. 31885-023) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... qPCR analysis was carried out in duplicate or triplicate on a Viia 7 real-time PCR system (Thermo Fisher). Fold changes in gene expression relative to either input (0hpi ...
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bioRxiv - Synthetic Biology 2020Quote: COS-7 cells were plated on poly-L-lysine coated coverslips and transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA quantification was done by real-time PCR on an ViiA 7 real-time PCR system (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (K0222 ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids pCAG-dsRed and Pak3K297R transfection was performed at 7 d in vitro (DIV) with 1 µg of DNA and 1.5 µl of Lipofectamine (Invitrogen), as described in manufacturer’s manual (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... was used for qRT-PCR in a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, USA). Data were analyzed by QuantStudio Software V1.3 (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Embedded samples were cut serially into slices of 7 µm using a MICRO HM355 (Thermo Fisher Scientific, Walldorf, Germany) and stained with Hematoxylin ...
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bioRxiv - Physiology 2020Quote: ... qPCR was run in a 384-well plate format on a ViiA 7 Real-Time PCR System (Applied Biosystems) using 2x SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Pathology 2019Quote: The infected rice sheaths were incubated with CellTracker™ Blue CMAC Dye (7-amino-4-chloromethylcoumarin, Molecular Probes, C2110) at a final working concentration of 10 μM for 2 h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... both Huh-7 cells and PHH were processed with the TaqMan Fast Advanced Cells-to-Ct Kit (Invitrogen, A35378), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...