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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 genome was amplified by using Ion AmpliSeq SARS-CoV-2 Research Panel (ThermoFisher Scientific, USA) that consists of two pools with amplicons ranging from 125 bp to 275 bp in length and covering >99% of the SARS-CoV-2 genome ...
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bioRxiv - Microbiology 2022Quote: ... The presence of recombinant virus was verified by immunostaining with a monoclonal antibody against the RABV nucleoprotein (FujiRebio, Cat# 800–092) and polyclonal antiserum against the SARS CoV/CoV-2 S1 domain (Thermo Fisher, Cat# PA581798). The filtered virus was then used to inoculate Vero cells seeded in Cellstack Culture Chambers (Corning ...
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bioRxiv - Microbiology 2021Quote: ... anti-SARS-CoV-2 (PA1-41098, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... SARS Cov-2 nucleocapsid (Thermofisher, 1: 1,000).
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bioRxiv - Molecular Biology 2021Quote: ... and spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). The supernatants containing the pseudotype viruses were collected 48 h post-transfection ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike ectodomain and the sRBD were transiently transfected in Freestyle ExpiCHO-S cells (Thermo Fisher). ExpiCHO cells were maintained and transfected according to the manufacturer using the “High Titer” protocol ...
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bioRxiv - Immunology 2021Quote: The codon optimized SARS-CoV-2 Wu01 spike (EPI_ISL_40671) was cloned into pCDNA™3.1/V5-HisTOPO vector (Invitrogen). SARS-2-S global strains (BavP1 EPI_ISL_406862 ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (RBD, 1:1,000, Invitrogen cat. no. PA5-114451). All primary antibodies were diluted in PBST + 5% milk and applied to membranes for ≥16 hours at 4 °C ...
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bioRxiv - Immunology 2022Quote: Codon-optimized SARS-CoV-2 spike protein expression plasmids were generated by cloning of gene fragments (Thermo Fisher) and/or site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit ...
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bioRxiv - Microbiology 2022Quote: ... biotinylated SARS-CoV-2 Spike trimer (Hexapro) was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... HIV reporter pseudoviruses expressing SARS-CoV-2 S glycoprotein and carrying the luciferase gene were produced in Expi293F cells (ThermoFisher Scientific) by co-transfection of the pNL4-3 ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 RBD (BEI Resources NR-52309) was biotinylated with Sulfo-NHS-LC Biotin (Thermo Fisher), then incubated with 1μm fluorescent neutravidin beads (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Genomics 2021Quote: ... NGS was performed using primer sets that tiled the entire SARS-CoV-2 genome (Ion AmpliSeq SARS-CoV-2 Research Panel, ThermoFisher), with library preparation and sequencing performed on Ion Chef and S5 ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein (kindly provided by Eric Fischer, DFCI) was coupled to fluorescent streptavidin beads (Thermo Fisher) and incubated with diluted plasma samples to allow antibody binding to occur ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike gene was amplified by PCR using Phusion Hot Start II DNA polymerase (Thermo Scientific™) and a set of primers recognizing sequences upstream and downstream of the spike gene (Forward ...
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bioRxiv - Microbiology 2020Quote: ... anti-SARS-CoV-2 S (Invitrogen, PA1-41165); anti-SARS-CoV-2 Nucleoprotein (ABIN129544) ...
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bioRxiv - Molecular Biology 2023Quote: ... SARS-CoV-2 N protein (MA1-7404, Invitrogen), TLR3 (ab62566 ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Bioengineering 2021Quote: ... and 2.5 μg of SARS-CoV-2-d18 spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Microbiology 2022Quote: Hybridoma Screening: Expi293F cells were transiently transfected to express SARS-CoV-2 spike (Wuhan) using Lipofectamine 3000 (L3000001, Thermo Fisher) and then incubated with supernatant from the hybridoma cell lines from each fusion ...
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Immunology 2022Quote: ... transfected HEK293T cells were stained with a rabbit polyclonal antibody that recognizes SARS-CoV-2 spike RBD (40592-T62, SinoBiological) and an Alexa Fluor 488-conjugated goat anti-rabbit IgG (A11034, Invitrogen). Cells were then acquired on a BD Accuri C6 plus and analyzed by FlowJo software version 10.6.2.
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bioRxiv - Immunology 2021Quote: HEK293T cells were transiently transfected with plasmids encoding full length SARS-CoV-2 spike variants using lipofectamine 3000 (L3000-001, ThermoFisher) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Codon-optimized sequences for the N or the spike S protein of SARS-CoV-2 were cloned into the pVAX1 expression plasmid (ThermoFisher) optimized for DNA vaccinations referred to as pVAX1-SARS2-N and pVAX1-SARS2-S 81 ...
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bioRxiv - Immunology 2020Quote: The prefusion ectodomain of the SARS-CoV-2 Spike protein was transiently transfected into suspension-adapted ExpiCHO cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Immunology 2021Quote: HEK293T cells were transiently transfected with plasmids encoding full length SARS-CoV-2 spike variants using lipofectamine 3000 (L3000-001, ThermoFisher) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Protein labelled cells were stained with biotinylated SARS-CoV-2 S1 (ACRO biosystems – S1N-C82E8) and detected with streptavidin AF647 (Invitrogen – S21374). Cells were stained with 7-AAD viability staining solution at 5 μl/1×106 cells to determine live cells ...
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bioRxiv - Immunology 2021Quote: ... pcDNA3.1-SARS CoV-2 SΔCT using lipofectamine 2000 (ThermoFisher). This approach was used to generate pseudoviruses specific to SARS-CoV-2 variant strains ...
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bioRxiv - Microbiology 2021Quote: ... antibodies targeting the SARS-CoV-2 nucleoprotein (N) (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 E protein was purchased from ThermoFisher Scientific (Catalog# RP-87682) ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 nucleocapsid (Invitrogen #PA5-81794; 1:1000), Spike (CST #56996S ...
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bioRxiv - Immunology 2023Quote: ... recombinant SARS-CoV-2 S protein (RP-87680, Invitrogen) or recombinant SARS-CoV-2 spike RBD protein (40592-V08H ...
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bioRxiv - Immunology 2021Quote: ... all PE labelled and prefusion SARS-CoV-2 S-Avi tag conjugated with streptavidin Alexa-Fluor 647 (Life Technologies). SARS-CoV-2-specific IgG+ memory B cells were sorted and seeded on MSC (mesenchymal stromal cells ...
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bioRxiv - Microbiology 2021Quote: ... and prefusion SARS-CoV-2 S with a biotinylated Avi-tag conjugated to Streptavidin Alexa-Fluor 647 (Life Technologies). SARS-CoV-2 S-specific IgG+ memory B cells were sorted by flow cytometry via gating for PE negative and Alexa-Fluor 647 positive cells ...
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bioRxiv - Immunology 2021Quote: ... and prefusion SARS-CoV-2 S with a biotinylated avi tag conjugated to Streptavidin Alexa-Fluor 647 (Life Technologies). SARSCoV-2 S-specific IgG+ memory B cells were sorted by flow cytometry via gating for PE negative and Alexa-Fluor 647 positive cells ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 and SARS-CoV were expressed in Hi5 cells using the Bac-to-Bac baculovirus system (Invitrogen). Briefly ...
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bioRxiv - Immunology 2021Quote: HEK293 cells were transiently transfected with SARS-CoV-2-S fragments expression vectors using Lipofectamine 2000 Transfection reagent (Thermo Fisher Scientific). Two days later ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Biochemistry 2022Quote: ... and transfected with 24 µg of plasmid encoding either the wildtype or E406W SARS-CoV-2 spike protein using Lipofectamine 2000 (Life Technologies). Four hours at transfection ...
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bioRxiv - Microbiology 2021Quote: A codon optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Microbiology 2022Quote: A codon-optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Microbiology 2021Quote: ... The positive control was addition of 10 μg/mL of SARS-CoV-2 spike protein antibody (pAb, T01KHuRb) (ThermoFisher, MA, USA) at the time of infection.
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bioRxiv - Microbiology 2021Quote: Point mutations to generate the mutants were introduced by site-directed mutagenesis of phCMV-SARS-CoV-2-Spike using Q5 polymerase (Thermo Scientific) and validated by sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...