Labshake search
Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... One millilitre of fresh sap or control sample was freeze-dried using a SpeedVac concentrator unit (model Savant SPD121P, Thermo Scientific, Waltham, MA, USA) at 40 □ ...
-
bioRxiv - Biochemistry 2022Quote: ... samples were incubated with 5 µg anti-FLAG antibody bound to Protein G Dynabeads (Invitrogen).
-
bioRxiv - Molecular Biology 2023Quote: ... added to anti-Flag or normal mouse IgG antibody-conjugated Protein G Dynabeads (Invitrogen 10003D), and rotated for 2 hours at 4 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lysates were adjusted to 1 mg/ml by IP buffer and protein complexes were immunoprecipitated by anti-FLAG magnetic beads (MilliporeSigma) or anti-HA magnetic beads (Thermofisher) at 40C for 4 h ...
-
bioRxiv - Plant Biology 2021Quote: ... a mouse anti-FLAG primary monoclonal antibody was used (anti-DYKDDDDK FG4R, 1 μg/mL overnight at 4 °C, Invitrogen), and horseradish peroxidase (HRP)-coupled goat anti-mouse IgG was used as a secondary antibody horseradish peroxidase (0.5 μg/mL for 1 hr at 21 °C ...
-
bioRxiv - Biophysics 2022Quote: ... pCDNA3-oep-FLAG was linearized with NotI and transcribed using an mMESSAGE mMACHINE T7 Kit (Invitrogen) according to the manufacturer’s recommendations.
-
bioRxiv - Developmental Biology 2021Quote: ... and Flag-fbl-D238A (250 pg/blastomere) mRNAs were synthesized with the mMessage mMachine kit (Ambion). To reveal the injected side ...
-
bioRxiv - Biochemistry 2022Quote: ... The cell suspension was transferred to a 96-well V-bottom plate in duplicate and fluorescently labeled with the anti-FLAG epitope tag antibody and a goat anti- mouse IgG secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: Mtb was grown as described above and processed for ChIP experiments using purified anti-PhoP (Alpha Omega Sciences) or anti-FLAG (Thermo Scientific) antibody and protein A/G agarose beads (Pierce ...
-
bioRxiv - Plant Biology 2021Quote: ... and added with 10 μl of the appropriate magnetic beads (anti-MyC beads, Thermo Fisher or anti-FLAG beads, Merck Millipore) and incubated for 1 hr at 4 °C ...
-
bioRxiv - Molecular Biology 2019Quote: ... with the addition of 30 µl of protein A (for anti-H3K9me) or protein G (for anti-FLAG) Dynabeads (Thermo Fisher). Library preparation was performed as described in (Homer et al. ...
-
bioRxiv - Immunology 2021Quote: ... Cell lysates were incubated with anti-FLAG or anti-Myc antibodies in the presence of protein G or A magnetic beads (Thermofisher Scientific) for 2 hours at 4°C while slowly rotating ...
-
bioRxiv - Developmental Biology 2021Quote: Primary antibodies were rabbit anti-eIF4E-5 (1:500; #4524, this work) or rabbit anti-FLAG (1:500; Invitrogen, PA1-984B), and secondary antibodies were Alexa Fluor 488-conjugated anti-rabbit (1:200 ...
-
bioRxiv - Cell Biology 2024Quote: ... Translation sites in fixed cells were labeled anti-Flag labeling (FUJIFILM, Wako) followed by Cy3 or Alexa 594 conjugated anti-mouse secondary antibodies (Jackson Immunoresearch and Invitrogen). In live cells ...
-
bioRxiv - Cell Biology 2021Quote: ... For immunofluorescence analysis (for method see below): Rabbit polyclonal anti-FLAG 1:500 (PA1-984B, Invitrogen) and mouse monoclonal anti- TOM20 1:400 (sc-17764 ...
-
bioRxiv - Microbiology 2022Quote: ... Supernatants were incubated with 50 µl FLAG magnetic beads (Pierce™ Anti-DYKDDDDK, Thermo Fisher Scientific) for 2 hours ...
-
bioRxiv - Plant Biology 2021Quote: ... Virp1 was detected by a monoclonal mouse anti-FLAG antibody (Thermo Fisher Scientific; 1:1,000 dilution). HRP-conjugated anti-mouse serum (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2023Quote: ... guinea pig anti-FLAG (1:500) (48) and IB4 (Alexa 647 conjugated) (Invitrogen, I32450, 1:500).
-
bioRxiv - Plant Biology 2023Quote: ... Flag-fusion protein purification was performed using Pierce™ Anti-DYKDDDDK Magnetic Agarose (Thermo Scientific, A36797) according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2024Quote: ... The supernatant was incubated with equilibrated anti-Flag antibody coupled magnetic beads (Thermo Scientific™, A36797) for 2 hours at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then incubated with an Alexa Fluor™ 488-labeled anti-FLAG antibody (L5; Invitrogen, Cat # MA1-142 ...
-
bioRxiv - Pathology 2021Quote: ... Flag-CHCHD10 S59L or Flag alone were transfected in HeLa cells using lipofectamine 2000 (Invitrogen). Twenty-four hours post transfection ...
-
bioRxiv - Plant Biology 2020Quote: ... Anti-FLAG antibodies were cross-linked to Dynabeads® M-270 Epoxy (Thermo Fisher Scientific, cat# 14311D) for immunoprecipitation ...
-
bioRxiv - Plant Biology 2020Quote: ... Anti-FLAG antibodies were cross-linked to Dynabeads® M-270 Epoxy (Thermo Fisher Scientific, cat# 14311D) for immunoprecipitation ...
-
bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
-
bioRxiv - Developmental Biology 2020Quote: ... anti-FLAG M8823 Millipore Sigma) conjugated to 50µl protein A/G magnetic beads (Dynabeads, Thermo Fisher Scientific) overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... IPs of FLAG-tagged PRRC2B and PRRC2B fragments were performed using anti-DYKDDDDK Magnetic agarose (ThermoFisher Scientific). Beads were mixed with 70 μl of SDS loading buffer (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mg of lysate was immunopurified with 20 μL of M2 Anti-FLAG magnetic beads (Thermo Fisher) and FLAG tagged material eluted using an excess of FLAG peptide (Sino biological ...
-
bioRxiv - Biophysics 2023Quote: ... while transgenes were detected using Millipore Sigma F3165 (anti-FLAG)—both of which were followed by Invitrogen 31430 (anti-mouse-HRP).
-
bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged ARL15 proteins were immunoprecipitated using 50 μL of Pierce Anti-DYKDDDDK Magnetic Agarose (ThermoFisher Scientific) for 2 hours at 4 ºC ...
-
bioRxiv - Immunology 2021Quote: ... cloned into pCMV-Flag vector (Invitrogen), and used as the plasmid standard after its identity was confirmed by sequencing ...
-
bioRxiv - Neuroscience 2021Quote: Reactions were undertaken with wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) purchased from ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293 stable cells overexpressing Flag-INTS118 and Flag-Ago215 were cultured in DMEM media (Gibco, #11965-084) containing puromycin and supplemented with 10% FBS (Atlas Biologicals ...
-
GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... The leftover samples were precipitated with anti-FLAG antibody which was prebound to Protein G Dynabeads (Thermo Fisher). The 50 µL of saved chromatin and immunoprecipitated DNA were de-crosslinked in a 65°C water bath overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... the anti-3×FLAG antibody used for immunoprecipitation was cross-linked to beads by dimethyl pimelimidate (Thermo Fisher). Immunoprecipitation was performed using mouse anti-FLAG M2 or mouse non-immune IgG (negative control ...
-
bioRxiv - Biochemistry 2023Quote: ... FLAG and Pgk1 antibodies were detected using a DyLight 800 coupled anti-mouse IgG secondary antibody (SA535521; Invitrogen). ALFA-tagged proteins were detected using an 1:1000 diluted rabbit anti-ALFA antibody (N1505 ...
-
bioRxiv - Microbiology 2023Quote: ... immunoprecipitation of Hfq-RNA complexes with an anti-Flag primary antibody using DynabeadsTM Protein A (Thermo Fisher, 10002D) overnight at 4 ℃ ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were transfected on chamber slides with pCDH-MGA-FLAG or pCDH-MGAΔDUF-FLAG using lipofectamine 2000 (Invitrogen). Cells were harvested 3 days post transfection and fixed for immunofluorescent staining.
-
bioRxiv - Physiology 2022Quote: ... pcDNA3.1(+)-FLAG-mGCNA was generated by cloning FLAG-mGCNA cDNA into pcDNA3.1(+) (catalog no. V790-20, ThermoFisher Scientific) between KpnI and EcoRI sites ...
-
bioRxiv - Cell Biology 2019Quote: ... FLAG-Con1mut-SPOP167-374 and FLAG-Con1-SPOPmut were subcloned into pcDNA™4/TO (Thermo Fisher Scientific) using BamHI and NotI ...
-
bioRxiv - Cell Biology 2019Quote: The coding sequence of EPO with a C-terminal Flag was cloned into pDEST-pcDNA5-BirA-FLAG (Invitrogen) using NEBuilder HiFi DNA assembly cloning kit (NEB) ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were seeded and transfected for 48h with pcDNA3.1-CISD1-FLAG or pcDNA3.1-CISD2-FLAG using Lipofectamine3000 (Invitrogen) as per manufacturer instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... the DYKDDDK epitope (FLAG; ThermoFisher, RRID: AB_2536846) 1:500 ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse Flag-Tag monclonal antibody(Thermo Fisher, WB 1:1000 dilution ...
-
bioRxiv - Molecular Biology 2021Quote: ... FLAG (1:1000, ThermoFisher Scientific #MA1-91878) and H3 (1:10,000 ...
-
bioRxiv - Microbiology 2023Quote: ... 100 μl of FLAG peptide (Thermofisher A36805) dissolved in PBS to a final concentration of 1.5 mg/ml ...
-
bioRxiv - Biochemistry 2024Quote: ... FLAG-tagged Fzd4 in pcDNA3.1 (Thermo Fisher), or both Tspan12 and Fzd4 (300 ng each receptor per mL of cells) ...
-
bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
-
bioRxiv - Molecular Biology 2021Quote: ... 12 µg anti-FLAG M2 antibody was coupled to 1.5 mg of magnetic Dynabeads M270 Epoxy (Thermo Fisher Scientific), as per manufacturer’s instructions ...