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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... MHC Class II-APC-eF780 (clone M5/114.15.2; Invitrogen), CD11c-PE-eF610 (clone N418 ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were treated with Exo-Sap (ExoSAP-IT™, Thermo Fisher, USA) to remove unutilized primers and dNTPs ...
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bioRxiv - Microbiology 2021Quote: Purified substrates and cleavage product mixtures were dephosphorylated by incubating with SAP (ThermoFisher, USA) at 37°C for 1 h according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... we purified the amplified DNA using USB Exo-SAP-IT (Affymetrix, Santa Clara, CA) and bidirectionally sequenced the amplicons using an ABI 3130 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting DNA from the PCR reaction was purified via EXO-SAP cleanup (Affymetrix). Individual EXO-SAP reactions consisted of 1.55ul dH2O ...
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bioRxiv - Microbiology 2021Quote: ... FLAG epitope was detected by mouse anti-FLAG M2 primary (Invitrogen, 1:1000 dilution) and goat anti-mouse IgG HRP secondary (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-V5 and Anti-Flag monoclonal antibodies were from Invitrogen, with all other primary antibodies from Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... at a concentration of 2μg per sample and anti-mouse MHC class II antibody conjugated to FITC (Clone: M5/114.15.2) (Invitrogen, USA) at a concentration of 5μg per sample ...
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bioRxiv - Microbiology 2021Quote: ... followed by purification using the Exo-SAP-IT PCR product clean-up reagent (Thermofisher, Australia) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... MHC-II IA/IE (M5/114, PerCP-eFluor710, Invitrogen 46532180), CD11b (M1/70 ...
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bioRxiv - Cell Biology 2023Quote: ... in M5 stabilization media (human endothelial SFM (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-flag (PA1-984B, Invitrogen, 1/1,000). ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Flag-tag (Thermo Fisher Scientific; MA1-91878), anti-GST (CST ...
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bioRxiv - Microbiology 2023Quote: ... rabbit monoclonal anti-Flag 8H8L17 (ThermoFisher; 1:500), mouse anti-HRP2 clone 2G12 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662), guinea pig anti-bovine insulin (Linco ...
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bioRxiv - Cancer Biology 2024Quote: ... 10ug of rabbit anti-FLAG antibody (Invitrogen; #740001) was added and allowed to incubate overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Flag-Tdrd3-N and Flag-Tdrd3-C were synthesized with an mMESSAGE mMACHINE SP6 kit (Ambion), and the resulting mRNAs (2 μg ...
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bioRxiv - Neuroscience 2022Quote: ... Background staining was blocked in 5% serum and incubated with either an anti-MHCII (1:500, M5/114.15.2; Thermo Fisher) or a pSer129 (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of antibody-conjugated beads/resin (FLAG: EZview Red ANTI-FLAG M2 Affinity Gel, Sigma-Aldrich; HA: Pierce Anti-HA Agarose, Thermo Scientific) for 30-60 in at 4°C with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... anti-NDUFA2 (this study), anti-FLAG (MilliporeSigma, F3165) and anti-ATPsynß (Life Technologies, A21351). Secondary antibodies used were goat anti-rabbit horseradish peroxidase (PI31460 from Pierce ...
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bioRxiv - Immunology 2022Quote: ... Super Bright 645-labeled MHC II (M5/114.15.2, Thermo Fisher Scientific), BV421-labeled PD-L1 (MIH5 ...
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bioRxiv - Immunology 2023Quote: ... Super Bright 645-labeled MHC II (M5/114.15.2, Thermo Fisher Scientific), BV421-labeled PD-L1 (MIH5 ...
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bioRxiv - Microbiology 2021Quote: ... Pull-down of GST-SeDJ-1 with Flag-SeASF1-28a was detected using an anti-Flag (Invitrogen). Each experiment was repeated at least three times.
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bioRxiv - Synthetic Biology 2020Quote: ... Anti-flag mouse monoclonal antibody-Dylight 650 conjugate (Thermofisher), targeting the bait-BirA ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was blotted with anti-flag (Invitrogen #740001) or anti-ubiquitin antibodies (Invitrogen #PA1-10023 ...
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bioRxiv - Microbiology 2023Quote: ... probed with mouse anti-FLAG primary antibody (Thermo Scientific) and goat anti-mouse HRP-linked secondary antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used are Mouse anti-Flag (Invitrogen, 1:1000); Rabbit anti-DDX6 (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... Incubations were performed with mouse anti-FLAG or mouse anti-V5 (ThermoFisher Scientific), and secondary anti-mouse IgG coupled with alkaline phosphatase (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-DYKDDDDK epitope (FLAG) tag (Thermo Scientific Pierce, MA1-91878), Anti-HA (Thermo Scientific Pierce ...
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bioRxiv - Microbiology 2022Quote: ... MX2-Flag proteins were detected using anti-Flag monoclonal M2 and secondary antibody conjugated to Alexa 594 (Invitrogen, A21203). Endogenous NUP358 was detected using rabbit anti-NUP358 antibody (Abcam ...
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bioRxiv - Evolutionary Biology 2023Quote: ... after enzymatic clean up with Exo-SAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) applying a 10x dilution of the Exo-SAP-IT but otherwise following the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Conjugated anti-HA-HRP (Cat#12013819001, MilliporeSigma) and anti-FLAG-HRP (Thermal Fisher Scientific) antibodies were used to detect HA- and FLAG-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... MHC II (I-A/I-E) (SuperBright645, Thermo Fisher, 1:200,M5/114.15.2), MHC I (H-2Kb/Db ...
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bioRxiv - Immunology 2019Quote: ... or anti-Flag antibody (FG4R, Thermo Fisher Scientific, MA1-91878-HRP) at 2 μg ml-1 (1:500 dilution from stock ...
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bioRxiv - Cell Biology 2021Quote: ... 10-20 μL rat anti-FLAG magnetic beads (Thermo Scientific A36797) were added to 1 mL of the centrifuged cell culture medium (above) ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were probed with rabbit anti-FLAG (1:5000; Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... to detect FLAG-Nav1.5 and rabbit anti-GFP (Thermo Fisher, G10362) to detect GFP-Nav1.5 ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were then mixed with anti-FLAG antibody (Thermal Fisher Scientific) for 1 h at 4 °C and incubated with protein-G magnetic beads (Bio-rad ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with a primary rabbit anti- FLAG antibody (Invitrogen) diluted 1:1500 for 3 hours ...
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bioRxiv - Immunology 2023Quote: ... Anti-flag antibody was pre-incubated with protein G Dynabeads (Invitrogen), and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Rabbit anti-FLAG (PA1-984B) was purchased from Invitrogen (Carlsbad, CA). Rabbit antibodies against Giantin (ab80864) ...
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bioRxiv - Biochemistry 2023Quote: ... 100 μl of M2 anti-Flag affinity gel (Thermo Fisher Scientific) was added to the soluble extract for 2 hours 30 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Immunological detection was performed with anti-FLAG-tag antibody (ThermoFisher Scientific) diluted 1:1000 in 1xTBST buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Precleared lysates were immunoprecipitated using M2 anti-Flag magnetic beads (Invitrogen) and protein eluted by boiling in loading buffer ...
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bioRxiv - Genetics 2023Quote: ... and mouse anti-FLAG (1:200) (MAI-91878, Thermo Fisher Scientific). Cells were labeled with Alexa Fluor 568-conjugated goat anti-rabbit IgG ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 1 hr at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... For staining FLAG tagged receptors we used anti-FLAG M1 antibody (1:100) labelled with DyLight594 (Thermo Scientific, Cat. no. 46412) dye in presence of 2 mM CaCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... syp-mCh and SSF-tagged opioid receptors using the lipofectamine method were incubated for 15 minutes with Alexa-647 conjugated anti-FLAG antibody (1/1000, M1 antibody from Sigma, Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher) before neurons were washed 3 times with HBS and mounted on the microscope in HBS ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were transfected with 15 μg of pMDS-TetOn3G-kozak-FLAG-GOI plasmids (containing FLAG-KRASWT or FLAG-KRASG12D or FLAG-KRASG12V or FLAG-KRASG12C as GOI) using Lipofectamine 2000 (Invitrogen, 11668-019) according to the manufacturer’s instructions in OPTI-MEM reduced serum medium (Gibco™ ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant His6-mEGFP-FLAG and MBP-eIF2A-His6-FLAG was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol ...