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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Flag eluates were analysed by SDS-PAGE on 4-12% polyacrylamide gels (NuPAGE, Invitrogen, Cat# NP0322BOX) with colloidal Coomassie staining (Roti-blue ...
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bioRxiv - Plant Biology 2022Quote: ... clarified extracts were incubated with FLAG-M2-conjugated Protein G Dynabeads (Thermo Fisher Scientific, Waltham, Massachusetts) for one hour ...
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bioRxiv - Biochemistry 2023Quote: Flag-ACKR3 was expressed in sf9 insect cells using the pFastBac baculovirus system (Thermo Fisher Scientific). Cells were grown in suspension in EX-CELL 420 medium (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... primary antibody and 488 (FLAG) Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer), then washed and imaged as described above.
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bioRxiv - Cell Biology 2023Quote: High titer virus expressing Flag-WRN was used to infect Hi5 insect cells (Thermo Fisher Scientific) at an MOI of approximately 10 ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti Flag-HA-V5 vector was modified from pLenti6-V5 gateway vector (Thermo Fisher scientific V49610) by Gibson cloning ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid pYES2-pGAL-FLAG-htt25QP-GFP-URA3 was linearized by restriction enzyme XagI (Thermo Fisher Scientific) to replace huntingtin (htt ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 ug of empty vector or FLAG-POLR3B was transfected per well using lipofectamine 2000 (Invitrogen). After 24h ...
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bioRxiv - Cell Biology 2024Quote: ... Ub-X-YFP-FLAG expressing vectors (0.2 µg) were used with the pcDNA 3.1 vector (Invitrogen) as the control vector to adjust the total plasmid amount by 0.5 µg per 35 mm dish ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-Igα Fab fragment was prepared with Pierce Fab Micro preparation kit (Thermo Scientific) using immobilized papain according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... we used the AlexaFluor 647 Tyramide Superboost (goat anti-rabbit) kit (ThermoFisher, Carlsbad, CA) as per the manufacturers’ protocol for detection of anti-ZikV E protein.
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies (Anti-OxPhos Complex Kit (Life technologies, cat. no. 457999) and Anti-MTCO1 antibody (Abcam ...
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bioRxiv - Plant Biology 2021Quote: ... SuperSignal West Dura Detection Kit was used to detect anti-tubulin (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: Anti-Ii for live immunostaining was labelled using Monoclonal antibody labeling kit (Molecular Probes) according to manufacturer’s protocol with AlexaFluor™-488 ...
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bioRxiv - Developmental Biology 2020Quote: ... The anti-SMA antibody was directly labeled using Zenon direct labeling kit (Invitrogen/ThermoScientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... anti-CD56 and LIVE/DEAD Fixable NearIR Dead cell stain kit (Thermo Fisher Scientific). Double p24 intracellular staining was performed after permeabilization using the BD Cytofix/Cytoperm kit (BD ...
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bioRxiv - Bioengineering 2024Quote: Anti-elastase activity was determined using the EnzChek™ Elastase Assay kit (Invitrogen™). A 1.5-fold dilution series of A1AT was prepared ...
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bioRxiv - Developmental Biology 2021Quote: ... Pogz-/- mESCs were transfected with pCAG-IRES-Puro-3×Flag-Pogz vector using Lipofectamine 2000 (Gibco, 11668019).
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for 1 hour (Sigma Aldrich M2 Flag at 1:1,000; Invitrogen 22C5D8 Pgk1 at 1:6,000; Roche 12CA5 HA at 1:2,000; Invitrogen R960-25 V5 at 1:2,000 ...
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bioRxiv - Biochemistry 2021Quote: ... The purity and concentration of p300-FLAG were assessed by 8% SDS-PAGE using BSA standards (ThermoFisher) and by western blot with an anti-FLAG antibody (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed overnight at 4°C with monoclonal α-FLAG (#31430, ThermoFisher Scientific; 1:10,000) antibody diluted in 1×TBS-T buffer containing 3% bovine serum albumin ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transfected with pcDNA3-FLAG-ATR and GFP (pLL3.7) in the presence of Lipofectamine 2000 (Invitrogen). 48 hours after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected with 200 ng of Flag-tagged FOXQ1 using Lipofectamine 2000 (Thermo Fisher, Waltham, USA). After 24 hours ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells were transfected with the pCS2-FLAG empty and protein coding constructs using Turbofect (ThermoFisher Scientific). After 48 hrs the cells were crosslinked with 1% formaldehyde (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg of Flag-Strep-Strep-(FSS)-tagged LRRK2RCKW cDNA and Lipofectamine 2000 reagent (ThermoFisher Scientific, USA) were used according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Adnp-/- mESCs were transfected with pCAG-IRES-Puro-3×Flag-Adnp vector using Lipofectamine 2000 (Gibco, 11668019). 48 hours later ...
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bioRxiv - Biochemistry 2021Quote: ... followed by a short linker FLAG-epitope tag and C-terminal Histidine6 was synthesised by GeneArt (Invitrogen). To generate a FLAG-free HuACE2 fusion ...
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bioRxiv - Biochemistry 2022Quote: ... 3×FLAG-BioID2 constructs were generated by performing a Gateway LR Clonase II reaction (Invitrogen, ThermoFisher Scientific) into the pDEST-pcDNA5-FRT/TO-BioID2-3×FLAG backbone (Hesketh et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 3×FLAG-BioID2 constructs were generated by performing a Gateway LR Clonase II reaction (Invitrogen, ThermoFisher Scientific) into the pDEST-pcDNA5-FRT/TO-BioID2-3×FLAG backbone (Hesketh et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and the resulting supernatants were subjected to coimmunoprecipitation (CO-IP) with antibodies against V5 or Flag (Invitrogen), before being pulled down by the BeyoMag™ Protein A+G beads (Beyotime [24] ...
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bioRxiv - Molecular Biology 2022Quote: ... Pogz−/− ESCs were transfected with pCAG-IRES-Neo-3×Flag-Pogz vector using Lipofectamine 3000 (Gibco, L3000008). 48 hours later ...
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bioRxiv - Plant Biology 2023Quote: ... pUBQ10:ZF108-FLAG-TRB3: the cDNA sequence of TRB3 was cloned into pENTR/D-TOPO vectors (Invitrogen), and then to the destination vector pMDC123-UBQ10:ZF-3XFLAG-GW by LR reaction (LR Clonase II ...
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bioRxiv - Molecular Biology 2023Quote: ... MITFmi-Flag in A375 cells for 24 hours before harvested by NativePAGE Sample Buffer (Thermo Scientific, #BN2003). We then performed Blue native PAGE (Wittig et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins of interest were labelled with antisera against the FLAG tag (Thermo Fisher Scientific, Waltham, MA, USA) and ATP5A (Abcam ...
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bioRxiv - Immunology 2024Quote: ... FLAG-tagged constructs were PCR amplified with Gateway adapters and cloned into pDONR221 using BP Clonase (Invitrogen). Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... for anti-eEF3 and anti-G-6-PDH secondary antibodies and developed with Pierce ECL plus western blotting substrate kit (Thermo Scientific 32132).
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bioRxiv - Microbiology 2021Quote: ... Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) 15 min prior to recordings ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
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bioRxiv - Microbiology 2021Quote: ... the murine vasculature was labelled with 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) were injected to label and visualize the human vessels ...
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bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530). Mice were infected by intravenous injection of 100 µl of the bacterial inoculum (107 CFU total ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were probed with species-specific donkey anti-rabbit or goat anti-mouse HRP-conjugated secondary antibody and detected with enhanced chemiluminescence (ECL) detection kit (Thermo Fisher, Carlsbad, CA).
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bioRxiv - Biochemistry 2019Quote: ... 1 μL of antibody (α-Flag-M2 antibody, Sigma-Aldrich / α-HA, Biolegend/ α-TAP, Thermo Fisher Scientfic) was added to the lysate and rotated for 3 hours ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Cell Biology 2019Quote: HEK293doxIRE1β cells were established by subsequent transduction of low passage HEK293 cells (obtained from ECACC) with lentiviral particles encoding the pLenti3.3 doxycycline-controlled regulator and pLenti6.3/V5/ERN2-FLAG (Invitrogen). ERN2-FLAG coding sequence was introduced by Gateway cloning ...
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bioRxiv - Biochemistry 2021Quote: Flag-tagged UCH37 (WT, C88A, ΔCTD, ΔCTD/C88A, EWI, EWI/C88A) were cloned into pcDNA5/FRT/TO (Invitrogen). Stable cell lines that inducibly express these proteins were constructed from Flp-InTM T-RExTM 293 Cells following manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... pVSV-G and pLJM1-3×Flag-BUD13-6HIS into HEK293T cells using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015). Lentiviruses were collected from conditioned media after 48 h co-transfection and filtered through 0.2μm syringe filter ...
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bioRxiv - Cell Biology 2021Quote: ... 2×106 RPE-1 cells were seeded per 10 cm dish and transfected with 3.75 μg Myc-PLK4 and 3.75 μg pcDNA5 FLAG-BirA construct using 15 μL Lipofectamine 3000/P3000 (ThermoFisher). Cells were harvested 24 h posttransfection ...
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bioRxiv - Microbiology 2022Quote: ... shRNAs targeting BUD23 or overexpressing ORF11-FLAG were seeded in triplicate in 12-well culture plates (Thermo Fisher) at a density of 3 × 105 cells per well and grown for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... RPE1 hTERT p53-KO FLAG-Cas9 and 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% FBS ...