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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: The double-stranded linkers were converted into single-stranded to successively allow the second strand synthesis by Shrimp Alkaline Phosphatase (1 U/μl SAP, catalog num. 78390, Affymetrix) and Uracil-Specific Excision Reagent (1 U/μl USER ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 x 105 cells were incubated with anti-FLAG antibody (Thermo Fisher), diluted 1:100 in PBS for 60 minutes at 10°C ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-FLAG (M2, WB 1:1,000, IF 1:500, ThermoFisher #F1804); chicken anti-synaptophysin (IF 1:500 ...
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bioRxiv - Genomics 2022Quote: ... and an anti-Flag-PE-Cy7 (Thermo Fisher Scientific, 25-6796-42) at a 1:300 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µg of the respective antibodies (Anti-ERα; ThermoFisher MA5-13065 or Anti-Flag; Sigma F3165) were added to the lysate and incubated on a rotating wheel at 4°C overnight.
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bioRxiv - Cell Biology 2021Quote: ... The rat anti-FLAG magnetic beads and the mouse anti-HA magnetic beads (Thermo Scientific 88836) were used for co-IP.
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-H3K27me3 (1:1000) and mouse anti-FLAG (1:200; Thermo Fisher Scientific #MA1-91878). Cells were labeled with Alexa Fluor 568-conjugated goat anti-rabbit IgG (Invitrogen #A-11036) ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted proteins were determined by Western blotting with anti-V5 and anti-FLAG antibodies (Invitrogen) and visualized by the horseradish peroxidase-conjugated secondary antibodies (ZSGB-BIO ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA underwent another final second round IVT step at 37 °C overnight and followed EXO-SAP treatment (Affymetrix 78200) for 15 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... pENTR-ATP6V1H-FLAG was produced using the pENTR/D-TOPO Cloning Kit (Invitrogen). pLenti-PGK-ATP6V1H-FLAG-Puro was subsequently generated by gateway cloning with LR clonase (Invitrogen) ...
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: ... pENTR-Flag-CASP6 was produced using the pENTR/D-TOPO Cloning Kit (Invitrogen) with the CASP6_OHu19575_pcDNA3.1(+)-N-DYK plasmid ...
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bioRxiv - Biophysics 2019Quote: ... FLAG beads (Thermofisher) were washed into assay buffer (100 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG (Invitrogen, 740001); V5 (Abcam ...
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bioRxiv - Biophysics 2021Quote: ... The antibodies used include anti-flag monoclonal antibody (mouse, Abcom) and anti-pgk1 Polyclonal Antibody (rabbit, Invitrogen). The secondary antibodies used include Peroxidase Affini Pure goatanti-mouse IgG and goat anti-rabbit IgG (Jackson) ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-afadin (clone D1Y3Z) or Alexa Fluor 488 anti-DYKDDDDK (flag) tag monoclonal antibody (L5, Life Technologies). The plasma membrane was stained with CF594 dye conjugated wheat germ agglutinin (Biotium) ...
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bioRxiv - Immunology 2021Quote: HEK293T cells were transfected with FLAG-Empty Vector (FLAG-EV) and FLAG-SP140 plasmids using Lipofectamine 2000 (Invitrogen) for 48 hours ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was loaded onto anti-DYKDDDDK (FLAG) affinity resin (Thermo Scientific, #. A36803), followed by extensive wash ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunopurified by an anti-FLAG antibody conjugated onto Dynabeads Protein G (Invitrogen). The beads were washed three times by wash buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated with 100 μL of anti-DYKDDDDK (FLAG) resin (Thermo Fisher Scientific) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were immunoprecipitated using anti-FLAG antibody coupled to Dynabeads Protein A (Invitrogen) for 3 hours at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... the protein extract was immunoprecipitated using an anti-FLAG antibody (Thermo Scientific, USA), and the co-immunoprecipitated proteins were detected using an anti-GFP antibody (Thermo scientific).
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bioRxiv - Cancer Biology 2023Quote: ... anti-FLAG epitope tag (DYKDDDDK) 1:500 (Thermo fisher, catalog no. PA1-984B); anti-Cyclin B1 (D5C10 ...
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bioRxiv - Microbiology 2024Quote: ... anti-FLAG (1:1000, M2 Sigma Aldrich F1804, or PA1-984B Thermo scientific), anti-GAPDH (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Immunology 2021Quote: HEK293T cells were seeded into 10-cm dishes and transfected with 25μg FLAG-Empty Vector (FLAG-EV) and FLAG-SP140 plasmids using Lipofectamine 2000 (Invitrogen). 48 hours after transfection ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: M5-T4L was expressed in Sf9 cells using the Bac-to-Bac Baculovirus Expression System (Invitrogen). Cells were infected at a cell density of 4.0 × 106 cells/millilitre with 10 μM atropine ...
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bioRxiv - Genomics 2023Quote: ... rabbit anti-H3K9me3 (1:200), rabbit anti-H3K27me3 (1:1000) (Frapporti et al., 2019) and mouse anti-FLAG (1:200) (Thermo Fisher Scientific Cat#MA1-91878 ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary (anti-flag mouse 1:200, Cell Signalling) and secondary antibody (anti-mouse Alexa 488 1:500, Thermofisher) were diluted in blocking buffer solution and incubated overnight at 4°C or 1 hour at RT ...
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bioRxiv - Genomics 2022Quote: ... anti-mouse 800 (only used in Figure 8E,F to detect mouse anti- FLAG) (1:5000, Invitrogen A32730). Anti-Sloth1 and Anti-Sloth2 antibodies (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Myc-Arhgef18 and/or the septin complex (Flag-SEPTIN2, Flag-SEPTIN6, Flag-SEPTIN7, GFP-SEPTIN9) on 6-well tissue-culture plates using Lipofectamine 3000 (Invitrogen) and harvested 18–24 h later ...
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bioRxiv - Cell Biology 2022Quote: ... The backbone for the Flag and Flag-His6 vectors is pcDNA3.0+ (Invitrogen), and that for the GFP vector is pEGFP-C1 (Takara Bio Inc).
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bioRxiv - Developmental Biology 2020Quote: ... the supernatant was incubated with anti-FLAG-M2 affinity agarose beads (Thermo Fisher Scientific) at 4°C O/N ...
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bioRxiv - Cell Biology 2022Quote: ... Thirty microliters of pre-equilibrated Anti-FLAG L5 Magnetic Beads (Thermo Fisher Scientific, A36798) and RNase A (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Remaining supernatant was used for immunoprecipitation using anti-FLAG M2 agarose beads (Thermo Fisher) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Fixed cells were incubated with rabbit anti-FLAG antibody (1:250 dilution, Thermo Fisher) overnight ...
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bioRxiv - Genetics 2020Quote: ... Zip3 ChIP was performed using anti-FLAG antibody and Protein G Dynabeads (Life technologies). For Msh5 western blot ...
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bioRxiv - Biochemistry 2021Quote: ... or a rabbit anti-FLAG antibody (cat# PA1-984B, 1:1000; Thermo Fisher Scientific) for 1 h at RT followed by an appropriate near-infrared secondary antibody (1:15,000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... M1 anti-FLAG antibody (In house) labeled with Alexa Fluor 647 (Thermo Fisher Scientific) was incubated with the cells at 2.5 μg/mL for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μg anti-FLAG preincubated with 50 μl protein G–conjugated Dynabeads (Thermo Fisher) was mixed with the supernatant for 3 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysates were incubated with pre-equilibrated Pierce™ anti-FLAG affinity resin (ThermoFisher) for 2 hours at 4°C with agitation ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on the anti-Flag affinity resin (Thermo Fisher Scientific, A36803) and incubated at 4 ℃ for 2h ...
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bioRxiv - Cell Biology 2024Quote: ... and FLAG were detected by immunoblotting with primary anti-CPY (1:5000; Invitrogen 10A5B5), anti-Dpm1 (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Flag antibody (ThermoFisher F1804); Alexa Fluor™ 555 Tyramide SuperBoost™ Kit ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... FLAG (PA1-984B, Invitrogen). Streptavidin-IRDye800 was from LI-COR (925-32230) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were stained with anti-FLAG 1:400 and anti-rabbit Alexa Fluor 488 1:1,000 (Invitrogen - A-11008) to identify transfected cells ...
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bioRxiv - Genomics 2022Quote: ... Indicated antibodies (anti-Flag, 1:2000, 12793S, Cell Signaling Technology; anti-Rabbit-IgG, 1:2000, G-21234, Thermo Scientific; anti-β-actin ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 μl of CHAMP buffer containing 4 nM Alexa488-conjugated anti-Flag antibody (Alexa Fluor 488 antibody labeling kit, Thermo Fisher; monoclonal BioM2, Sigma-Aldrich) was injected to wash off unbound RNP and label DNA-bound RNP complex ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... For pharmacology experiments M2 and M5 mAChR DNA was cloned into a pEF5/FTR/V5 vector (Invitrogen) using the Flp-In-CHO cell system (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: The newly constructed pcDNA5-FRT-HaloTag7-Flag and pcDNA5-FRT-Rpn13(1–128)-HTP-FLAG-FLAG plasmids were co-transfected with the pOG44 plasmid (Invitrogen #V6005-20) containing Flp recombinase into Flp-In 293 cells (Invitrogen R750-07 ...
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bioRxiv - Plant Biology 2021Quote: 5 μg of RNA from 5-day-old seedlings was first treated with 1 unit of Shrimp Alkaline Phosphatase (SAP; USB Products, Affymetrix, Inc.; Cleveland, OH, USA) in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen ...