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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The presence of SARS-CoV-2 RNA was determined by conventional reverse transcription PCR using the SuperScript®III One-Step RT-PCR System with Platinum® Taq DNA Polymerase kit (Invitrogen) in a 25μl reaction volume containing 12.5μl of 2X buffer ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 subgenomic RNA of the E gene was reverse transcribed and copy numbers quantified by real-time PCR using the SuperScript III OneStep RT-PCR Kit (Invitrogen Life Technologies) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed and viral genome copies quantified by real-time PCR using the SuperScript III OneStep RT-PCR Kit (Invitrogen Life Technologies) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: One-step RT-PCR was performed on 2ul total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232-1583 of the PPRV N gene using published primers NP3 and NP4 (Couacy-Hymann et al 2002 ...
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bioRxiv - Immunology 2021Quote: ... Real-time RT-PCR was used for mRNA quantification (7500 Fast Real-Time PCR system, Applied Biosystems Inc., Foster City, California, USA). All primers were designed to cover exon-exon boundaries to avoid amplification of genomic DNA ...
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bioRxiv - Microbiology 2020Quote: ... The amount of the intracellular RNA was estimated by real-time quantitative PCR performed with the RNA UltraSense™ One-Step Quantitative RT-PCR System (Applied Biosystems) by using the following primer sets ...
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bioRxiv - Immunology 2019Quote: ... Real-time RT-PCR was used for mRNA quantification (7500 Fast Real-Time PCR system, Applied Biosystems Inc., Foster City, California, USA). All primers were designed to cover exon-exon boundaries to avoid amplification of genomic DNA ...
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bioRxiv - Biochemistry 2021Quote: ... The viral titers were determined by the qRT-PCR method using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific) and a sequence of primers and probe for the E gene53 ...
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bioRxiv - Neuroscience 2021Quote: We performed gene expression analysis by quantitative real-time RT-PCR (qPCR) using SYBR Green PCR Master Mix (Thermo Fisher Scientifics, #4309155) and the ViiA™ 7 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... the RNA was subjected to reverse transcription PCR for cDNA synthesis according to the RT-PCR kit (Applied Biosystems, Cat. No. 4368814). The relative gene expression was measured according to 2-ΔΔCT methods ...
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bioRxiv - Genetics 2022Quote: ... The SARS-CoV-2 infection was screened and confirmed by at least one quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) assay with the Applied Biosystems 7500 Fast RT-PCR System (Thermo Fisher Scientific), using a protocol previously described for the detection of 2019 novel Coronavirus (2019-nCoV ...
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bioRxiv - Synthetic Biology 2022Quote: ... situated between nsP4 and the viral 3’ untranslated region (UTR) were reverse-transcribed and PCR-amplified using the SuperScript™ IV One-Step RT-PCR System (ThermoFisher, #12594025) and the 26S-F primer and pooled SinRev primer ...
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bioRxiv - Microbiology 2022Quote: ... The viral cDNA was amplified from extracted RNA through one-step reverse transcription-PCR amplification by using SuperScript III One-Step RT-PCR system with High Fidelity Platinum Taq DNA Polymerase (Invitrogen, Life Technologies, USA) with degenerate primers (10uM MBTuni-12M and MBTuni-13 ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR was performed using 1 µl of eluted RNA and a SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) as described by the manufacturer.
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription quantitative PCR (RT-qPCR) assays were performed using the Quantstudio 5 Real-time PCR system (Applied Biosystems, Waltham, MA, USA). Premixes were prepared for each amplification reaction using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted with NucliSens easyMAG (BioMérieux) and quantified by quantitative real time PCR (RT-qPCR) using SuperScript™ III Platinum™ One-Step qRT-PCR Kit (Invitrogen) in a CFX96 Thermal Cycler (BIORAD) ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription and PCR were performed on 35ng of total RNA per well using AgPath-ID One-Step RT-PCR Reagents (4387424, Thermo Fisher Scientific) with the following TaqMan primer-probe sets (all Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time reverse-transcription PCR (RT–qPCR) assays were performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). The primer sequences were listed in S1 Table ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative RT-PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO Life Science) with the StepOnePlus Real-time PCR System (Thermo Fisher Scientific). Results were normalized using the means of Actb and Gapdh as reference genes ...
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bioRxiv - Microbiology 2023Quote: ... real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using Taqman probe (Mm00458221 ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed with duplicates on a MicroAmp™ Fast Optical 96-Well Reaction Plate (Themo Fisher Scientific) using the QuantStudioTM 5 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time reverse transcription polymerase chain reactions (RT-PCRs) were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Life Technologies). Data were normalized to housekeeping genes (Tubulin ...
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bioRxiv - Physiology 2023Quote: ... Quantitative RT-PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO Life Science) with the StepOnePlus Real-time PCR System (Thermo Fisher Scientific), as in our previous studies (26 ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was reverse transcribed using Superscript III One-Step RT PCR Kit with Platinum® Taq High Fidelity enzyme followed by amplification using Platinum® PCR SuperMix High Fidelity (Invitrogen). A 2200bp amplicon spanning gag and protease was generated by nested PCR as detailed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time quantitative PCR (RT-qPCR) was performed on the QuantStudio™ 5 Real-Time PCR System 384-well (Applied Biosystems, #A28140) using SenSMix™ SYBR® No-ROX buffer (Meridian Bioscience) ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA was subjected to reverse transcription PCR for cDNA synthesis according to the RT-PCR kit (Applied Biosystems, Cat. No. 4368814). The relative gene expression was measured according to 2−ΔΔCT methods ...
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bioRxiv - Microbiology 2024Quote: ... and the rRT-PCR was performed using the Path-ID™ Multiplex One-Step RT-PCR Kit (Thermo Fisher, Waltham, MA, USA) under following conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR of the cDNA was performed using a primer set for the N gene of RSV (45) and ACTB (Applied Biosystems, TaqMan Assay ID: Hs99999903_m1) as a housekeeping gene ...
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bioRxiv - Plant Biology 2020Quote: ... and primers in 10 μL of reaction and an ABI7500 real-time PCR system (Life Technologies). The expression of each gene was normalized to the expression of Actin and quantified using the 2-ΔCt method ...
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bioRxiv - Bioengineering 2020Quote: ... and primer-probe assays (IDT) on a Step One Plus Real-Time PCR System (Applied Biosystems). The AR-V7 primer-probe assay included ...
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bioRxiv - Immunology 2019Quote: ... qRT-PCR was performed using commercially available Taqman primer/probes for cd5 and ubc (Thermo Fisher).
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bioRxiv - Microbiology 2019Quote: ... qPCR was performed using specific primers and the SYBR Green PCR master mix (Applied Biosystems, USA) in a 20 μl reaction system ...
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bioRxiv - Genetics 2019Quote: ... and 0.2 µM each primer in a 7500 Fast Real-Time PCR System (Applied Biosystems, USA). RT-qPCR conditions were as follows ...
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bioRxiv - Immunology 2021Quote: ... and TaqMan Primer/Probes was run on QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) with standard settings ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR was performed with target-specific primers using the TaqMan Gene Expression Assay (Applied Biosystems) or the Fast SYBR Green Assay (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... and the primers listed in table S4 on a Quantastudio Real-time PCR instrument (Applied Biosystems). ☐-actin levels were used to normalize input amounts ...
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bioRxiv - Immunology 2021Quote: ... Primers and diluted cDNA samples were prepared with Power SYBR Green PCR Master Mix (Applied Biosystems). Amplification cycles using the iQ5 system (BioRad ...
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bioRxiv - Biochemistry 2020Quote: ... SuperScript® III First-Strand Sythesis SuperMix for qRT-PCR and primers were purchased from Invitrogen, Carlsbad.
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bioRxiv - Cell Biology 2021Quote: ... The following primers were used for amplification on a StepOne Real Time PCR system (Applied Biosystems) (OPTN ...
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bioRxiv - Systems Biology 2021Quote: ... Primers were resuspended in 15μL PCR master mix (0.2μL Phusion High Fidelity DNA Polymerase (Thermo Scientific), 0.4μL 10 mM dNTP Solution (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was subjected to PCR using SYBR Select Master Mix using gene specific primers (Applied Biosystems). Fold increases in gene expression with respect to control samples (indicated in each figure legend ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was subjected to PCR using SYBR Select Master Mix using gene specific primers (Applied Biosystems). Fold increases in gene expression with respect to control samples (indicated in each figure legend ...
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bioRxiv - Immunology 2021Quote: ... 0.4 mM of each primer forward and SYBR Green PCR Master Mix (4309155E, Applied Biosystems Ins.). All quantification reactions were controlled with a melting curve and primer efficiency was tested with standard curves ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP and mCherry expression was assessed using specific primers(39) and SybrGreen PCR reagents (Applied Biosystems). Quantitative real-time PCR (QRT-PCR ...
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bioRxiv - Immunology 2020Quote: ... with specific primers (Supplementary Table S1) in an ABI 7300 Real Time PCR System (Applied Biosystems). Results were analyzed using SDS2.4 software.
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bioRxiv - Neuroscience 2020Quote: ... 0.5 µL Illumina PCR Primer Cocktail and 4.5 µL of 0.6x 2xSYBR green I (Invitrogen, S7563). From the results of this qPCR we determined that each library required 4-5 additional PCR cycles in order to achieve optimal amplification without compromising library complexity ...
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bioRxiv - Cell Biology 2022Quote: ... the primers listed in Supplementary Table 11 and a 7500 Real Time PCR System (Applied Biosystems). Gene expression levels were quantified relative to GAPDH expression using the ΔCt method ...
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bioRxiv - Plant Biology 2019Quote: ... and gene-specific primers (Table S2) on a StepOne Plus Real Time PCR machine (Life Technologies).
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bioRxiv - Immunology 2019Quote: ... 0.4 mM of each primer forward and SYBR Green PCR Master Mix (4309155E, Applied Biosystems Ins.) All quantification reactions were controlled with a melting curve and primer efficiency was tested with standard curves ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μl of qPCR primer and 10 μl of SYBR® Green PCR reagents (Life Technologies) were added to each well ...