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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... amplified with indicated primers using the Power SYBR Green PCR Master Mix (Thermo Fisher, # 4367659), and analyzed by QuantStudio 5 Real-Time PCR (Thermo Fisher).
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bioRxiv - Physiology 2022Quote: ... each specific primer at 200 nM and SYBR Green PCR Master Mix (Thermo Fisher Scientific), following the manufacturer’s conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Nextera primers matching those used in the first PCR step and SYBR Green I (Invitrogen), the following qPCR program was performed ...
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bioRxiv - Cancer Biology 2024Quote: ... and following probes and primers on a 7300 Real-Time PCR System (Applied Biosystems, CA). Primer/probes for mouse TNF-a gene were purchased from Applied Bioscience (Mm00443258_m1) ...
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bioRxiv - Cell Biology 2023Quote: To remove leftover primers 6 μL ExoSAP-IT For PCR Product Clean-Up (Affymetrix – 78200) was added and the sample was incubated at 37°C for 15 min and then chilled and kept at 4°C.
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bioRxiv - Genetics 2024Quote: ... and then sequenced using the PCR primers on an ABI 3730 capillary sequencer (Life Technologies). The sequence data were analyzed using Unipro UGENE v1.32.0 (49–51)
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bioRxiv - Microbiology 2020Quote: ... The RT-qPCR was assessed on a Real-time PCR StepOne Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). PCR thermal cycling conditions had an initial step at 94 °C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Semi-quantitative radioactive PCR (RT-PCR) was carried out in the presence of 32P-dCTP with AmpliTaq DNA polymerase (Thermo Fisher), and real-time quantitative RT-PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Semi-quantitative radioactive PCR (RT-PCR) was carried out in the presence of 32P-dCTP with AmpliTaq DNA polymerase (Thermo Fisher), and real-time quantitative RT-PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative RT-PCR was performed and analyzed according to the protocols of StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific) with 2× SYBR green master mix (Quanta Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNAs were subjected to RT-PCR using the Dream Taq Green PCR master mix (Thermo Scientific, San Jose, CA, USA). Quantitative real time PCR (q-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... reverse-transcribed with High Capacity Kit (Life Techonologies) and analyzed by quantitative RT-PCR with a Step One Real-time PCR system as described above (Applied Biosystems).
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bioRxiv - Neuroscience 2019Quote: ... H3R and D1R were amplified by real-time (RT)-PCR using 1 μL cDNA and power SYBER green PCR Master Mix (Applied Biosystems) on a 7500 Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... RNA samples were subjected to qRT-PCR analysis using AgPath-ID One-Step RT-PCR Kit (Applied Biosystems; Thermo Fisher Scientific). The primer and probe sequences targeting SARS-CoV-2 N gene included ...
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bioRxiv - Microbiology 2020Quote: ... RNA samples were subjected to qRT-PCR analysis using AgPath-ID One-Step RT-PCR Kit (Applied Biosystems; Thermo Fisher Scientific). The primer and probe sequences targeting SARS-CoV-2 N gene included ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted TNA samples were analyzed by reverse transcription quantitative PCR (RT-qPCR) using TaqMan probes on the QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) as described previously (Osman et al. ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed and viral genome copies quantified by real-time PCR using the SuperScript III OneStep RT-PCR Kit (Invitrogen Life Technologies) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 subgenomic RNA of the E gene was reverse transcribed and copy numbers quantified by real-time PCR using the SuperScript III OneStep RT-PCR Kit (Invitrogen Life Technologies) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using Sensitive RT HS-PCR Mix SYBR (A&A Biotechnology) on One Step Plus Real-Time PCR System (Applied Biosystems). The list of qPCR primers is provided in Table S8.
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative RT-PCR was performed according to the protocol of Power SybrGreen PCR Master Mix (Applied Biosystems, Foster City, CA, USA). The detailed protocol is described in Table S3.
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bioRxiv - Neuroscience 2022Quote: ... Real-time polymerase chain reaction (RT-PCR) was performed on a 7500 Real-time PCR System (Applied Biosystems, Carlsbad, CA, USA) with Universal SYBR Green Master Mix (4913914001 ...
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bioRxiv - Microbiology 2022Quote: The reverse transcription quantitative PCR (RT-qPCR) assays for the detection of TMPRSS2 were performed using the Quantstudio 5 Real-time PCR system (Applied Biosystems). Premixes were prepared for each amplification reaction ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qRT-PCR) reactions were implemented with Platinum™ SYBR™ Green qPCR SuperMix - UDG (Invitrogen, Thermo Fisher Scientific) using the ABI 7500 real-time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qRT-PCR) reactions were implemented with Platinum™ SYBR™ Green qPCR SuperMix - UDG (Invitrogen, Thermo Fisher Scientific) using the ABI 7500 real-time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Real time analyses by two-step RT-PCR was performed for quantification of miRNA on a 7500 REAL TIME PCR SYSTEM (Applied Biosystems) or Bio-Rad CFX96TM real time system using Applied Biosystems Taqman chemistry based miRNA assay system.
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bioRxiv - Immunology 2020Quote: Single cells were sorted in 96-well PCR plates in 9 µl of RT/pre-amp mix from the CellsDirect One-Step qRT-PCR Kit (Life Technologies) and were kept at −80°C at least overnight ...
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bioRxiv - Microbiology 2020Quote: ... All rRT-PCR testing was done using Superscript III One-Step RT-PCR System with Platinum Taq Polymerase (Thermo Fisher Scientific). Each 25-μL reaction contained 12.5 μL of the reaction mix ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR assay was performed using the AgPath-ID One-Step RT-PCR Buffer and Enzyme Mix (Life Technologies, Carlsbad, CA). AgPath-ID One Step RT PCR reactions were carried out in 20µl reactions with 1X RT-PCR Buffer ...
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bioRxiv - Immunology 2022Quote: ... To evaluate the expression levels of certain genes we used TaqMan MGB gene expression assays and TaqMan Universal PCR Master Mix on Viia7 RT-PCR machine (Applied Biosystems). Gene expression was standardised to an endogenous reference gene 18s rRNA (Hs99999901_s1 ...
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bioRxiv - Plant Biology 2020Quote: ... To confirm that ndufb10 and ndufa5 KO lines were lacking target gene expression Reverse-Transcriptase PCR (RT-PCR) was performed on cDNA (RevertAid Reverse Transcriptase; Thermo Scientific) synthesized after RNA extraction.
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bioRxiv - Molecular Biology 2020Quote: Real time analyses by two-step RT-PCR was performed for quantification of miRNA and mRNA levels on a 7500 REAL TIME PCR SYSTEM (Applied Biosystems) or Bio-Rad CFX96™ real time system using Applied Biosystems Taqman chemistry based miRNA assay system ...
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bioRxiv - Immunology 2020Quote: ... and 5’-AATGGGAAGTCACGAAGGTG-3’(reverse) and quantitative real-time RT–PCR was conducted on a StepOne Plus PCR instrument (Applied Biosystems). The NF-κB-luciferase (Luc2p ...
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bioRxiv - Developmental Biology 2020Quote: ... 1:10 cDNA dilution was used for each reaction of quantitative RT-PCR analysis performed on an ABI Prism HT 7900 real-time PCR detection system (Applied Biosystems) equipped with SDS software version 2.4 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed using a Fast SYBR Green Master Mix using the OneStep instrument (Applied Biosystems). The obtained values were normalized to either HPRT1 or GAPDH genes ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded in technical quadruplicates for q-RT-PCR on a QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher, 4485691). The ΔΔCT method was used to provide gene expression values after normalising to the known reference gene B2M ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded in technical quadruplicates for q-RT-PCR on a QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher, 4485691). The ΔΔCT method was used to provide gene expression values after normalising to the known reference gene B2M ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We did One-step real-time RT-PCR (Step-One Plus real-time PCR thermocycler, Applied Biosystems, Foster City, CA, USA), using total nucleic acids extracted by Trizol® (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time quantitative PCR (RT-qPCR) reactions were performed in the Quant Studio6 Flex Real Time PCR System (ABI, Thermo Fisher) using the SYBR Select PCR Master Mix (ABI ...
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bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR was performed in 96-well plates using an ABI PRISM 7300 Real-time PCR System (Applied Biosystems) and the relative gene expression for the different genes was calculated from the crossing threshold (Ct ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription PCR (RT-PCR) was carried out after cDNA synthesis using the High Capacity cDNA reverse transcription kit (Applied Biosystems). Real-time quantitative reverse transcription PCR (qRT- PCR ...
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bioRxiv - Genomics 2023Quote: The real-time quantitative PCR (RT-qPCR) assays were performed using the QuantStudieo 5™ Real-Time PCR System (Applied Biosystems). Amplifications were carried out in 25 μL reaction solutions containing 12.5 μL 2x PerfeCTa SYBR green SuperMix (2X ...
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bioRxiv - Bioengineering 2023Quote: ... Total RNA was then reverse-transcribed to cDNA and real-time polymerase chain reaction (PCR) was performed using the Applied Biosystems StepOne Plus RT-PCR with SYBR Green Master Mix (Applied Biosystems). Murine gene names and primer sequences are listed in the Supporting Information (Table S1) ...
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bioRxiv - Cancer Biology 2024Quote: ... was synthesized from 1000 ng of RNA using the Maxima First Strand cDNA Synthesis Kit for PCR with reverse transcription (RT–PCR) (Thermo Fisher). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-quantitative Reverse Transcription PCR (RT-qPCR) was carried out using Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, USA) and primers for germ layer differentiation and pluripotency marker genes (Suppl ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression levels were determined by quantitative real-time polymerase chain reaction (RT-PCR) (StepOnePlusTM Real-Time PCR System; Applied Biosystems) with the 2-ΔΔCT method [35] ...
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bioRxiv - Neuroscience 2023Quote: ... The expression of specific mRNAs was assayed using fluorescence-based quantitative real-time PCR (RT-qPCR) (Fast-SYBR PCR Master Mix; Applied Biosystems). Quantification reactions were performed in Duplicate for each sample using the ‘delta-delta Ct’ method ...
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bioRxiv - Microbiology 2023Quote: ... Quantification of transcripts was carried out by quantitative reverse transcription polymerase chain reaction (RT-PCR) using SYBR Green PCR Master Mix (Applied Biosystems) in a QuantStudio 6 Flex Real-Time instrument (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed in MicroAmp optical 384-well plates using the QuantStudio 6 Flex RT-PCR system (Thermo Fisher). The default thermocycling program was used (95ºC for 10 min ...
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bioRxiv - Immunology 2023Quote: ... Quantitative RT-PCR was performed using Universal SYBR Green Supermix with StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA). Data was graphed as a 2ΔΔ CT fold change with the gene expression data being normalized to the house keeping gene RPS18.
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bioRxiv - Genetics 2023Quote: ... Quantitative real-time RT-PCR (qPCR) was performed on the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA), using the SYBR-Green Master Mix (Applied Biosystems ...