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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... RT-PCR reactions were performed in a 20 μL mixture containing 1× Power SYBR™ Green PCR Master Mix (Thermofisher Scientific), 0.5 μmol/L of each primer ...
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bioRxiv - Immunology 2023Quote: ... Quantitative polymerase chain reaction(PCR) was conducted with PerfeCTa SYBR-Green FastMix-Rox(Quanta-Biosciences) and the StepOnePlus rt-PCR system(Applied Biosystems). We used the 2-ΔCT method for statistical analysis ...
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bioRxiv - Developmental Biology 2024Quote: ... and quantitative real-time PCR (RT-qPCR) was carried out in a 7500 PCR System using TaqMan Fast Advanced Master Mix (Applied Biosystems). The following Taqman assays probes were used ...
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bioRxiv - Microbiology 2024Quote: ... Real-time RT-PCR was performed in 96-well plates using an ABI PRISM 7300 Real-time PCR System (Applied Biosystems), and the relative gene expression for the different genes was calculated from the crossing threshold (Ct ...
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bioRxiv - Microbiology 2019Quote: ... / Bac1115Probe (5’-CAACGAGCGCAACCC-3’) primer and probe set targeting a conserved region of bacterial 16S rRNA genes using a QuantStudio3 platform (Thermo Fisher Scientific, Waltham, MA) (ref ...
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bioRxiv - Neuroscience 2024Quote: ... using a custom probe/primer set (ITRJET(FAM)-APMF3G9) created via Custom TaqMan® Gene Expression Assays Design Tool (Applied Biosystems, Foster City, CA) and designed to bind to a fragment of the expression vector including 3’-ITR and JeT promoter sequence ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time qRT-PCR reactions were set up using FastStart Universal SYBR Green Master Mix (Thermo Fisher Scientific) and thermal cycling was performed using a StepOnePlus (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... PCR products (0.4 μL of each set) were added to 10 μL of HiDi™-Formamide (Applied Biosystems), containing 0.3 μL of the GeneScan™HD 500 ROX™ Size Standard (Applied Biosystems) ...
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bioRxiv - Physiology 2022Quote: ... and qPCR reactions were set up and performed on the StepOne-Plus Real-Time PCR System (Applied Biosystems) as previously described (Wilson ...
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bioRxiv - Neuroscience 2024Quote: ... 140 ng cDNA was used to set-up a PCR reaction using Platinum® Taq mix (Invitrogen™) (Supplementary Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... The coding sequence of OsSWEET11b was PCR amplified without STOP codon (primers: Table S1) using Phusion High-Fidelity PCR polymerase (Thermo Fisher Scientific) and cloned into the Gateway donor vector pDONR221 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Diluted cDNA was used for qRT-PCR using gene specific primers (Table S2) and Power SYBR Green PCR Master Mix (Applied Biosystems, A25742). Gapdh was used as an endogenous control.
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bioRxiv - Cancer Biology 2022Quote: ... This single-stranded cDNA was then used to perform quantitative real-time PCR (qRT-PCR) with specific primers using PowerUP SYBR Green 2x Master Mix (Thermo Fisher Scientific) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR amplification was performed with barcoded reverse PCR primers in 25 cycles in Taq DNA Pol reaction buffer 1 × (Thermo Fisher Scientific), with 1 μM of each reverse barcoded primer and forward primer (Table SI1) ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was performed with specific primers (Supplemental Table S4) using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Cell Biology 2024Quote: ... was performed on 12 ng cDNA with SybrGreen PCR mastermix (LifeTechnologies) and specific primers (supplementary Table 2) and run in an ABI? real-time PCR machine (Applied Biosystems, ThermoFisher Scientific). The raw data was analysed through -ΔCt as described in Supplementary Methods.
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative PCR analyses were performed using primers listed in Supplementary Table 1 and SYBR Green PCR Master Mix (Applied Biosystems; ref 4385614). The relative mRNA levels were calculated using the 2-ΔΔCt method [76] ...
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bioRxiv - Neuroscience 2020Quote: ... Synthetic oligos were cloned into the GW/EmGFP-miR vector (Invitrogen), which contains sequences derived from miR-155 (see Fig ...
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bioRxiv - Molecular Biology 2019Quote: ... We also measured miR-375-3p (Thermo Fisher Assay ID #00564), which was not included on the array ...
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bioRxiv - Cancer Biology 2019Quote: ... Anti-miR-146a (AM-34a) (ID: AM11030) were obtained from Ambion, CD24 siRNA (a pool of 3 target specific siRNAs ...
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Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancerbioRxiv - Cell Biology 2022Quote: ... Cells were transfected using either a miR-206 miRNA mimetic (Thermofisher) or negative control mimetic #1 (Thermofisher ...
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bioRxiv - Molecular Biology 2019Quote: ... 10-nM mirVana hsa-miR-21 specific inhibitor (Thermo Fisher Scientific), or mirVana miRNA inhibitor Negative Control (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and miR inhibitor negative control (CAT#4464076) were purchased from Ambion/Life Technologies.
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected using either a miR-206 miRNA mimetic (Thermofisher) or negative control mimetic #1 (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... synthetic cel-miR-39-3p (Thermo Fisher Scientific, Waltham, MA, USA) was added to samples during the cell/MP lysis step as a spike-in control ...
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bioRxiv - Cancer Biology 2024Quote: For the functionality validation of miR-34a mimic (MC11030, Thermo Fisher), PC3 cells were seeded onto individual wells of a 96-well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-miR miRNA inhibitors (AM10801, Ambion, Applied Biosystems, Foster City, US) and corresponding controls were reverse transfected in HepG2 cells in triplicates as described above and a glucose uptake assay (Glucose Uptake-GloTM Assay ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-miR miRNA inhibitors (AM10801, Ambion, Applied Biosystems, Foster City, US) and corresponding controls were reverse transfected in HepG2 cells in triplicates as described above and a glucose uptake assay (Glucose Uptake-GloTM Assay ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... including the predicted target sequences of miR-891b (Thermo Fisher Scientific), were used to construct 3’UTR reporter vectors ...
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bioRxiv - Biochemistry 2021Quote: ... 500 ng RNA of the total isolated RNA were used as a template for the synthesis of cDNA using Oligo(dT) primers and the Superscript™ II RT protocol (Invitrogen). qPCR was performed using ORA qPCR Green ROX L Mix (HighQu ...
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bioRxiv - Molecular Biology 2021Quote: Reverse Transcription (RT) was performed with 2 μg total RNA and using 3.2 μg random hexamer primers (ThermoFisher Scientific, Cat# SO142) and Transcriptor Reverse Transcriptase (Roche ...
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bioRxiv - Genetics 2019Quote: ... This RNA was converted to cDNA using an oligo dT primer (RT-Uni) and Superscript III (ThermoFisher Scientific, Waltham, MA, USA) for use as a PCR template ...
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bioRxiv - Microbiology 2021Quote: ... The product was used as a template for reverse transcription (RT) with a linker-specific reverse primer using TaqMan Reverse Transcription Reagents from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Aliquots of the RNA preparations were subjected to RT with primer PI (5’-AGGCTTGCAAACGGAGTCTAA-3’) and RevertAid reverse transcriptase (Thermo Scientific). RT products were amplified by PCR with Thermus thermophilus DNA polymerase (Biotools ...
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bioRxiv - Cell Biology 2021Quote: ... Individual cells were sorted into 96-well plates containing 4uL of lysis buffer with specific barcoded RT primers (final concentration: 0,2% Triton, 0,4 U/μL RNaseOUT (Thermofisher Scientific, Waltham, Massachussetts), 400nM idx_RT_primers) ...
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bioRxiv - Immunology 2021Quote: ... RRV cDNA was generated from 10 μl of serum derived RNA or 1 μg of tissue derived RNA using a sequence-tagged (indicated with lower case letters) RRV-specific RT primer (5′-ggcagtatcgtgaattcgatgcAACACTCCCGTCGACAACAGA-3′) with SuperScript IV reverse tran-scriptase (Life Technologies). RRV genomes were quantified by RT-qPCR using a tag sequence-specific reverse primer (5′-GGCAGTATCGTGAATTCGATGC-3′ ...
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bioRxiv - Molecular Biology 2022Quote: Immunopurifed RNA (45 ng) was reverse transcribed (RT) with U6 and U2 snRNA reverse primers using SuperScript™ III Reverse Transcriptase (ThermoFisher), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1μg of RNA was reverse transcribed to cDNA by using oligo-dT primers and MMLV-RT (Invitrogen, Waltham, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Reverse Transcription (RT) was performed with 1 μg total RNA and using 0.2 μg random hexamer primers (ThermoFisher Scientific, Cat# SO142) and 200 U SuperScript® IV Reverse Transcriptase (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: 10 ng of total RNA was reverse transcribed with MegaplexTM miRNA-specific stem-loop RT Primers Human Pool A v 2.1 (Thermo Fisher Scientific) and TaqMan® MicroRNA reverse transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse Transcription (RT) was performed on 500 ng of RNA with random primers and SuperScript II Reverse Transcriptase (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR reaction was performed using the ViiA7 Real-Time PCR System (Thermo Fisher, USA) in 384-well plates ...
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bioRxiv - Plant Biology 2019Quote: Viral progeny was analyzed by reverse transcription (RT)-PCR using RevertAid reverse transcriptase (Thermo Scientific) and Phusion DNA polymerase followed by electrophoretic separation of the amplification products in 1% agarose gels that were stained with ethidium bromide ...
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bioRxiv - Cell Biology 2020Quote: Real-time RT-PCR was performed in 96-well optic plates (Applied Biosystems, N801-0560) in an Applied Biosystems ABI7500 thermocycler ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-qPCR was performed on an ABI 7900HT Fast Real Time PCR machine (Applied Biosystems) and analysed on SDS Biosystem software ...
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bioRxiv - Immunology 2021Quote: ... cDNA was obtained using Verso cDNA kit Reverse Transcription for RT-PCR (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT–qPCR was performed with Real-Time PCR System (Applied Biosystems, Quantstudio 7 Flex), using SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Thermal cycling was run on a Step-One Plus RT-PCR thermal cycler (Applied Biosystems) with the following cycle parameters ...