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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: H9 human embryonic stem cells were maintained in Essential 8 (E8) medium (Gibco A1517001) on iMatrix-511 (Matrixome ...
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bioRxiv - Immunology 2024Quote: ... M2 macrophages were plated into Lab-Tek 8-chamber slides (Nunc, Thermo Fisher Scientific) and were stained with PKH26 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole cell extracts were resolved by 8-16% Tris-glycine polyacrylamide gel (Invitrogen XP08165BOX), transferred to PVDF membranes with iBlotTM 2 transfer device (Thermo Fisher IB21001 ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Propionic acid (99%, ACROS Organics, CAS 79-09-4), 1,4-diaminobutane (99% ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Cat. No. M6494, ThermoFisher Scientific, Waltham, MA, USA) to differentiate the range of toxic and nontoxic palmitic acid concentrations ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Pathology 2020Quote: ... citric acid (Fisher Scientific, Cat. No. A104-3, Hampton, NH), sodium phosphate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled to a pH of 7.4 with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cell Biology 2020Quote: ... Tissue was then transferred to 1% fibronectin-coated chambered slides (μ-Slide 8 Well Glass Bottom, Ibidi #80827, for live imaging; Nunc™ Lab-Tek™ II Chamber Slide with removable wells ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate volume was increased to 1 ml and 8 μg of antibody were used in combination with Protein G Dynabeads M-280 (Thermo Scientific) as described above ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested before the cultures reached OD600 = 1 and were labeled with 8 mg/ml EZ-Link Sulfo-NHS-LC-biotin (ThermoFisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS (pH 8) and incubated with 1 mg/ml of EZ-link Sulfo-NHS-biotin (Thermo Fisher) in PBS (pH 8 ...
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bioRxiv - Genetics 2022Quote: ... or one of five Hsa21 genes (100 ng/well; 1 row, 8 wells or technical replicates per unique cDNA) using Lipofectamine 2000 (Invitrogen, 11668030) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Microbiology 2020Quote: ... The pH was adjusted to pH 8-8.5 with 1 M NaHCO3 and 50 µg of Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2021Quote: ... Solubilized membranes were mixed 1:1 with binding buffer (20 mM Tris pH 8/1 M NaCl/20 mM imidazole) and loaded onto a HisPur Ni-NTA (Thermo Scientific) column equilibrated with 20 mM Tris pH 8/750 mM sucrose/500 mM NaCl/10 mM imidazole/0.2% DDM (equilibration buffer) ...
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bioRxiv - Biochemistry 2020Quote: ... High-resolution mass spectra and MS/MS fragmentation pattern of 1-8 were recorded using a Q Exactive Plus mass spectrometer (Thermo Scientific). Chromatography for determination of amino acyl hydroxamate was performed on a Waters ACQUITY H-class UPLC system coupled to a Xevo TQ-S micro (Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Cell Biology 2022Quote: ... and 4.8 µL cDNA (diluted 1:20 prior to use) was added to each well of qPCR plates (REF 4346906, Life Technologies). Samples were run on a StepOnePlus™ RT-qPCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... Differentiated iNeurons were then cryogenized as aliquots of 1 · 106 to 8 · 106 cells in heat inactivated fetal bovine serum (FBS, 10270-106, Thermo Fisher) containing 5% v/v dimethyl sulfoxide (DMSO) ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Genetics 2023Quote: ... IP and input DNA were purified using ChIP elution buffer (250 mM NaCl, 1% SDS, 10 mM Tris-Cl pH 8, 1mM EDTA) and incubation with proteinase K (ThermoFisher Scientific) overnight at 65°C ...
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bioRxiv - Genomics 2023Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Biochemistry 2023Quote: ... Freshly isolated mitochondria were resuspended in 50 μl of click-it lysis buffer (50 mM TRIS-HCl pH 8, 1% SDS, 250 U/mL Universal Nuclease [Thermo Fisher]) and incubated on ice for 15 min ...
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bioRxiv - Immunology 2024Quote: ... cleanup beads were used (0.8:1 beads/sample ratio) and then eluted in 10 µL of DNase/RNase-Free Distilled Water (10977- 049, ThermoFisher®). cDNA was quantified by the Quant-iT PicoGreen dsDNA Assay Kit (P7581 ...
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bioRxiv - Cell Biology 2022Quote: ... 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Cat. no. 15630-080,) and 1% penicillinstreptomycin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH = 8.5) (ThermoFisher Scientific, Waltham, MA), 4-benzoylbenzyl-trimethylammonium chloride (PLPP ...
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bioRxiv - Neuroscience 2023Quote: ... at 1% B with Solvent A as 0.1% formic acid in water (Thermofisher Optima LS118-4) and Solvent B as 0.1% formic acid in acetonitrile (Thermofisher Optima LS120-4) ...
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bioRxiv - Bioengineering 2023Quote: ... was reduced on the 78 ± 3 nm cores using 1 ml of 1 mM L-ascorbic acid (AA) (Fisher Scientific), making core@shell structures ...
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bioRxiv - Biophysics 2021Quote: ... the soluble fraction was filtered and incubated for 3 h at 4 °C with ∼6 ml of HisPurTM Ni-NTA resin (Thermo Fisher Scientific). The beads were subsequently washed with ∼400 ml lysis buffer (without protease inhibitors or Arginine/Glutamic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies #D1306) was used for nuclei staining in conjunction with secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (Invitrogen, # D1306)
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bioRxiv - Bioengineering 2020Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen D1306) for 30 min at room temperature.
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bioRxiv - Cell Biology 2021Quote: ... DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (Invitrogen, D1306). Protein G–Sepharose (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific) staining according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) was then added immediately at a concentration of 1:1000 to label DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... or 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probes) was applied as a nuclear counterstain ...