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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Bacteria were aliquoted in brain heart infusion media (BHI; Fisher Scientific, DF0037-17-8) and stored at −80°C to decrease variability between replicates due to freeze-thaw cycles ...
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bioRxiv - Microbiology 2023Quote: ... HCT-8 cells were plated on 12-mm-diameter glass coverslip (Thermo Fisher Scientific) in 24-well tissue culture plates and incubated until confluent ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were grown in Essential 8 Flex Basal Media (Gibco, A28583-01, Thermofisher) on vitronectin-coated plates (07180 Stemcell technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were grown in Essential 8 Flex Basal Media (Gibco, A28583-01, Thermofisher) on vitronectin-coated plates (07180 Stemcell technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected for 8 h with indicated plasmids in Lipofectamine 3000 (Invitrogen) and then allowed for 24-h recovery from transfection by changing a fresh medium before subsequent experiments.
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bioRxiv - Cell Biology 2023Quote: ... per 106 cells and 0.8 (low) or 8 U (high) U RNase I (Ambion) per 106 cells added ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial suspensions were sonicated and centrifuged in a F21-8×50y rotor (Thermo Scientific) at 4°C for 15 min at 20,000 xg ...
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bioRxiv - Neuroscience 2023Quote: ... with 50 ul transferred into each well of an 8-well chamber slide (Nunc™ Lab-Tek™ II Chamber Slide™ System ...
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bioRxiv - Molecular Biology 2023Quote: Cells were seeded into an I-Bidi 8-well chambered slide (ThermoFisher, Carlsbad, California) at a density of 4 x 104 in 500 µL DMEM containing 10% FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then transferred to 8-well imaging plates (No. 1.5 glass. ThermoFisher Scientific) and incubated at 37°C in 250 μls of 1 μM FLIPPER-TR probe (Spirochrome ...
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bioRxiv - Cell Biology 2023Quote: H9 (WA09) cells were maintained with Essential 8 Flex media (#A28558501, Thermo Fisher Scientific) in feeder-free conditions on vitronectin (VTN-N ...
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bioRxiv - Biochemistry 2023Quote: ... with 8 kD dialysis membrane (SpectraPor 7 8000 Dalton MWCO; 11425919, Fisher Scientific UK). For syntaxin-6 the buffer used for dialysis was 10 mM Na-phosphate,150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... The primary cultures were maintained for 8 days in Neurobasal-A medium (Gibco™) supplemented with 1% rat serum (Biowest) ...
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bioRxiv - Neuroscience 2024Quote: ... B5525]) and subjected to SDS-PAGE using 8-16% Tris-glycine gels (Invitrogen, EC60485BOX). Proteins were transferred onto polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Microbiology 2023Quote: Human ileocecal adenocarcinoma (HCT-8) cells (ATCC) were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM Tris pH 8) for Western blot experiments or in TRIzol (Invitrogen, 15596026) for RNA extraction ...
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bioRxiv - Microbiology 2023Quote: ... proteins were separated by SDS-PAGE (Bolt NuPAGE 8% Bis-Tris plus gel; Invitrogen) and transferred using 1X liquid transfer buffer (Invitrogen) ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: 8-well cell culture chamber slides (Lab-Tek II; #154534, Thermo Fisher Scientific, USA) were analyzed using a DeltaVision RT microscope (GE Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... On day 8 cells were frozen in fetal bovine serum (FBS, Gibco, 10437-028) supplemented with 10% DMSO ...
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bioRxiv - Microbiology 2024Quote: ... and filtrates pH was adjusted to pH 8 (Thermo Scientific Eutech pH 700 Meter) with HCl ...
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bioRxiv - Microbiology 2024Quote: ... 30μg of protein was loaded in an 8-16% SDS-PAGE gel (ThermoFisher Scientific) and transferred to an Immobilon-P PVDF membrane ...
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bioRxiv - Microbiology 2024Quote: ... Denatured lysates were separated on NuPage 8-16% Tris-glycine Midi Protein gels (Invitrogen) and transferred to polyvinylidene difluoride membrane (Immobilon ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were maintained on Matrigel-coated plates in Essential 8 Medium (E8; Life Technologies). 0.5 mM EDTA was used for lifting cells while passaging ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were seeded in 8-well glass incubation chambers (5000 cells/well, Nunc 155409) with 0.2 ml of cultured medium ...
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bioRxiv - Molecular Biology 2024Quote: ... They were cultured in Essential 8™ Medium (E8) (Thermo Fisher Scientific, Cat. #A1517001) on Vitronectin XF™ (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Propionic acid (99%, ACROS Organics, CAS 79-09-4), 1,4-diaminobutane (99% ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Cat. No. M6494, ThermoFisher Scientific, Waltham, MA, USA) to differentiate the range of toxic and nontoxic palmitic acid concentrations ...
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bioRxiv - Pathology 2020Quote: ... citric acid (Fisher Scientific, Cat. No. A104-3, Hampton, NH), sodium phosphate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled to a pH of 7.4 with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2024Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2024Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cell Biology 2020Quote: ... Tissue was then transferred to 1% fibronectin-coated chambered slides (μ-Slide 8 Well Glass Bottom, Ibidi #80827, for live imaging; Nunc™ Lab-Tek™ II Chamber Slide with removable wells ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate volume was increased to 1 ml and 8 μg of antibody were used in combination with Protein G Dynabeads M-280 (Thermo Scientific) as described above ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested before the cultures reached OD600 = 1 and were labeled with 8 mg/ml EZ-Link Sulfo-NHS-LC-biotin (ThermoFisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS (pH 8) and incubated with 1 mg/ml of EZ-link Sulfo-NHS-biotin (Thermo Fisher) in PBS (pH 8 ...
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bioRxiv - Genetics 2022Quote: ... or one of five Hsa21 genes (100 ng/well; 1 row, 8 wells or technical replicates per unique cDNA) using Lipofectamine 2000 (Invitrogen, 11668030) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Microbiology 2020Quote: ... The pH was adjusted to pH 8-8.5 with 1 M NaHCO3 and 50 µg of Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2021Quote: ... Solubilized membranes were mixed 1:1 with binding buffer (20 mM Tris pH 8/1 M NaCl/20 mM imidazole) and loaded onto a HisPur Ni-NTA (Thermo Scientific) column equilibrated with 20 mM Tris pH 8/750 mM sucrose/500 mM NaCl/10 mM imidazole/0.2% DDM (equilibration buffer) ...
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bioRxiv - Biochemistry 2020Quote: ... High-resolution mass spectra and MS/MS fragmentation pattern of 1-8 were recorded using a Q Exactive Plus mass spectrometer (Thermo Scientific). Chromatography for determination of amino acyl hydroxamate was performed on a Waters ACQUITY H-class UPLC system coupled to a Xevo TQ-S micro (Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Cell Biology 2022Quote: ... and 4.8 µL cDNA (diluted 1:20 prior to use) was added to each well of qPCR plates (REF 4346906, Life Technologies). Samples were run on a StepOnePlus™ RT-qPCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... Differentiated iNeurons were then cryogenized as aliquots of 1 · 106 to 8 · 106 cells in heat inactivated fetal bovine serum (FBS, 10270-106, Thermo Fisher) containing 5% v/v dimethyl sulfoxide (DMSO) ...
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bioRxiv - Immunology 2024Quote: ... cleanup beads were used (0.8:1 beads/sample ratio) and then eluted in 10 µL of DNase/RNase-Free Distilled Water (10977- 049, ThermoFisher®). cDNA was quantified by the Quant-iT PicoGreen dsDNA Assay Kit (P7581 ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...