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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: hESCs (H9 cells) were cultured in Essential 8 Medium (Thermo Fisher Scientific, A1517001) on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... Organoids were grown in 24 well plates (or 8-well chambered coverglass, Nunc LabTek II ...
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bioRxiv - Developmental Biology 2024Quote: ... hPSCs were maintained as undifferentiated colonies in Essential 8 medium (Thermo Fisher, A1517001) on vitronectin (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... aliquots of parasite cultures were incubated in 8 µM Hoechst 33342 (ThermoFisher Scientific) for 20 min at room temperature and then fluorescence was measured using a CytoFlex S (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... and phagocytosis was assessed by live imaging in 8-well chambers (Nunc 155409) coated with collagen IV (Corning 354233 ...
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bioRxiv - Biochemistry 2024Quote: ... and 8 U of RNA were digested with 800 U RNaseI (AM2294, Invitrogen) in 80 µL reaction volume at 22 °C and 1’400 rpm for 1 h.
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bioRxiv - Genetics 2024Quote: ... PCR products were visualized by running PCR reactions on 8% TBE gel (Invitrogen) and staining with SYBR Safe (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were rinsed in 3% acetic acid (Fisher Scientific) for 3 minutes and incubated at room temperature for 30 minutes in 1% Alcian Blue 8GX (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... and 3-mercaptopropionic acid were purchased from ACROS ORGANICS. Hydrogen tetrachloroaurate(III ...
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bioRxiv - Developmental Biology 2023Quote: ... acidified to pH2-3 by trifluoroacetic acid (Thermofisher, 85183), and desalted with a Pierce C18 spin column (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: The metabolism of 4-hydroxyphenylacetic acid (p-hydroxyphenylacetic acid; Acros Organics, Product code: 121710250) and gentisic acid (2,5-dihydroxybenzoic acid ...
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bioRxiv - Cell Biology 2021Quote: ... Serial block-face imaging was performed at 1.8 KeV and 800 pA using the ICD and TLD detector in backscattered mode and the ASV 4 software (Thermo Fisher Scientific, Hillsboro, Oregon, USA). The block was milled at a current of 750 pA with 10 nm slices and images were acquired at a resolution of 10 nm/pixel with a dwell of 5 μs and a line average of 2 for a total z-depth of about 15 μm ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Biophysics 2019Quote: ... N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine] (Thermo Fisher Scientific, Inc., Waltham, MA). Y-ATRAM (Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed twice in 1 × PBS and lysed in 8 M urea supplemented with 1 × Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, USA). Insoluble cellular material was removed by centrifugation at 18,000 ×g for 20 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001, respectively). The cells were maintained so that once the iPSC colonies were confluent ...
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bioRxiv - Cell Biology 2023Quote: mTERT-immortalized mouse CCD cells were cultured as described previously (8, 9) in 1:1 DMEM/F-12 (Gibco GlutaMAX™; Fisher #10565018) with 5% FBS (Gibco One Shot™ ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA#4: 5’-AUAGCGUUUCUUCUAACUGGGCAGC-3’ (Invitrogen). siRNAs #2 and #4 significantly decreased the mRNA levels to the greatest extent and both had the same mitotic phenotypes ...
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bioRxiv - Biophysics 2023Quote: ... 7-Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin (CPM) (Invitrogen. USA), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% Triton X-100 in PBS for 20 minutes, then were actin stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) nucleic acid stain (Thermo Fisher Scientific, USA) in PBS for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... All sections were mounted with 50μl of ProLong Gold Antifade mounting media containing a fluorescent nucleic acid dye 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, P36931) before imaging ...
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bioRxiv - Immunology 2019Quote: ... or 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher).
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bioRxiv - Neuroscience 2019Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies) staining was added to visualize nuclei.
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bioRxiv - Neuroscience 2021Quote: ... 4’,6- diamidino-2-phenylindole (DAPI; Invitrogen D1306) was added during the secondary antibody incubation at a concentration of 700 ng/ml ...
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bioRxiv - Neuroscience 2019Quote: ... counterstained with DAPI (4’,6-diamidino-phenylindole, Invitrogen) for 45 min (both steps at room temperature) ...
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bioRxiv - Pathology 2021Quote: ... 4’,6-Diamidino-2-phenylindole (DAPI, D21490, ThermoFisher) stain was done for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen). Confocal analyses of stained slides were performed using a TCS SP8 Laser Scanning Spectral Confocal Microscope (LEICA Microsystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher) and ActinRed™ 555 ReadyProbes™ (Molecular Probes ...
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bioRxiv - Neuroscience 2022Quote: DAPI (4′,6-diamidino-2-phenylindole, D1306, Invitrogen) staining was performed by incubation at 1:500 for 10 min in DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... DAPI (4’, 6-diamidino-2-phenylindole, ThermoFisher Scientific) was applied to samples at a concentration of 0.1μg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... DAPI (4’ −6’ -diamino-2-phenylindole, dilactate; Invitrogen) was used for nuclear staining ...
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bioRxiv - Bioengineering 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher) was added for 30 min at room temperature before the secondary antibodies were washed out in PBS 3x for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, D3571) was added for 5 minutes in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... DAPI (4-6-diamindino-2-phenylindole; Molecular Probes) was used to detect DNA ...
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bioRxiv - Microbiology 2020Quote: ... washed in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA, USA) two times ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 25-35ug nuclear extract was separated on home-made SDS-PAGE gels or NuPAGE™ 3-8% Tris-Acetate gels (Life Technologies, for large Mediator subunits). Gels were blotted onto nitrocellulose membranes using the Trans-Blot Turbo transfer system (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The inhibition in cell viability exerted by different treatments for one or three cycles of 48h was evaluated by using the soluble tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay (Life Technologies, Eugene, USA). In living cells ...
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bioRxiv - Zoology 2020Quote: ... 2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-23491-52-3 (Hoechst 33342, trihydrochloride, trihydrate, Life Technologies, H3570) in water for 20 min ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 3.2 μg of p0G44 (pSB2380) and 1 μg of pSB2860/pSB2863 were mixed with 8 μL of Lipofectamine2000 (Invitrogen 11668027) in 400 μL of DMEM (no supplements ...
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bioRxiv - Molecular Biology 2021Quote: 1×104 A549 cells were grown in poly(D-lysine)-coated borosilicate glass Lab-Tek 8-well chambers (Thermo Scientific) and stained with MitoTracker Green FM (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37C incubator (ThermoFisher). Prior to plating ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). For σ28 purification ...
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bioRxiv - Cell Biology 2021Quote: ... dried peptide sample was resuspended in 200 mM HEPES (pH 8) and incubated for 1 h at room temperature with one of the TMT10-plex reagents (ThermoFisher) solubilized in 100% anhydrous ACN ...