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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... 1×104 transfected cells were seeded into each well of 8-well Lab-Tek™ II chamber slides (ThermoFisher Scientific) for immunostaining analysis ...
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bioRxiv - Biochemistry 2022Quote: ... coated Corning dishes at 37°C in a 5% CO2 incubator and gently dissociated for passaging at 1:8 ratio every second day with Stem Pro Accutase (Gibco). Cells were washed with Dulbecco’s Phosphate Buffer Saline cell culture grade.
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bioRxiv - Microbiology 2021Quote: ... HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day or more prior to infection ...
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bioRxiv - Immunology 2021Quote: HEK203-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day or more prior to infection ...
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bioRxiv - Immunology 2020Quote: HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day prior to infection ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Genomics 2021Quote: ... The tubes were then supplemented with 630 μl chloroform/isoamyl alcohol (24:1, pH 8, Acros Organics, Cat. No.: 327155000) and bead beat at setting 6 for 40 sec using the FastPrep-24™ Classic Instrument (MP Biomedicals ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples at the Cambridge laboratory were stained with a 1:2500 dilution (8 µM) of Hoechst 33342 (peak excitation: 351 nm, peak emission: 461 nm) (#62249, ThermoFisher) for 30 mins ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mL Lysis Buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific), and a 45-s process in a FastPrep-24 (MP Biomedicals ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated in 20 mL 33 loading buffer diluted to 13 in lysis buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific)) ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resuspended in 500µL of Sonication buffer (SDS 1%, 10mM EDTA, 50mM Tris HCl pH 8) with an anti-protease cocktail (A32965, ThermoFisher) and sonicated for 15” at 4°C (30s ON ...
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bioRxiv - Cancer Biology 2023Quote: ... Stably transduced cell lines were generated by infection over-night in the presence of 8 μg/ml polybrene and 48 hours after infection were selected with 1 μg/mL Puromycin (Gibco) for three days ...
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bioRxiv - Genomics 2023Quote: ... medium containing 2 µg/ml puromycin and 8 µg/ml polybrene (HCT116-Cas9) and 1 µg/ml (iPSC-iCas9) or no (NPCs- iCas9) puromycin (GIBCO) was exchanged ...
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bioRxiv - Neuroscience 2023Quote: ... SARK and HMW tau extracts (8 ng total tau per well) were incubated with 1% lipofectamine 2000 transfection reagent (ThermoFisher) in opti-MEM (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fed in full media for 1 h prior to 8 h starvation in Earle’s Balanced Salt Solution (EBSS; Life Technologies).
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bioRxiv - Biophysics 2023Quote: ... Then we detached them using trypsin and plated a 1:10 dilution in DMEM on an 8-well chamber (Lab-Tek 1.0 borosilicate glass, Thermo Fisher), which we let grow overnight ...
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bioRxiv - Physiology 2023Quote: Animals were culled using a schedule-1 method followed by injection of the common bile duct with 1 mg/mL collagenase NB 8 (Serva) in RPMI 1640 (Gibco) and pancreas dissection ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher) and loaded onto a MonoQ 5/50 GL column for anion exchange chromatography ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher), then concentrated to ∼0.5 mL and loaded onto a Superose 6 Increase 10/300 column pre-equilibrated in GF150 buffer ...
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bioRxiv - Cell Biology 2020Quote: 4.5 g/l glucose (Life technologies), 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2020Quote: ... 4.5 mM DTT (Thermo Fisher Scientific), 0.45% Nonidet P-40 (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 4.5 g/L Glucose (Gibco, A2494001) and 2mM EDTA ...
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bioRxiv - Genomics 2019Quote: ... 4.5 μl Proteinase K (Thermo Scientific), and 12 μl 5M NaCl was then added and the ChIP was incubated at 65°C overnight to revert formaldehyde cross-linking ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 4.5 (Ambion, cat n° AM9722). RNA was precipitated with isopropanol ...
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bioRxiv - Immunology 2021Quote: ... high glucose 4.5 g/L (Gibco, Life Technologies Italia ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4.5 g/liter D-glucose (Gibco), 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... 4.5 g/L D-glucose (Gibco). The human breast cancer cell lines ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4.5 g/liter D-glucose (Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemias were determined on day 3 by staining with SYBR Green 1 nucleic acid stain (Invitrogen) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Microbiology 2019Quote: ... day 1 (24 hours) and day 3 (72 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... DiIC18(3) dye (6 mg; Invitrogen, Carlsbad, CA, USA) was dissolved in 99.5% methylene chloride (300 μL ...
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bioRxiv - Immunology 2019Quote: ... 3-4 drops of blood were collected in 1 mL MEM medium (Gibco) supplemented with 2000 U/L heparin (Ratiopharm) ...
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bioRxiv - Genomics 2022Quote: ... The cells were then transfected with 3 μg of the plasmid constructs using 4.5 μL of Lipofectamine 2000 (Thermofisher), as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes (min ...
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bioRxiv - Developmental Biology 2023Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes at room temperature (RT) ...
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Grand Island, NY, USA) for 15 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... then incubated in 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Bioengineering 2022Quote: ... cell nuclei were stained with 1:1000 DAPI (4′,6-Diamidino-2-Phenylindole, Dihydrochloride, ThermoFisher) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific). Alexa Fluor 568 phalloidin was used to stain filamentous actin (F-actin ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). Antibodies are listed in the Key Resources Table ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were stained with 4’,6-diamidino-2-phenylindole (DAPI, 1:10K, Thermo Fisher Scientific) and mounted onto Superfrost slides (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and all sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher, 1:1000).
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bioRxiv - Immunology 2020Quote: ... the sections were stained with DAPI (4′, 6-diamidino-2-phenylindole; 1 μg/ml; Invitrogen). In the immunofluorescence study ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was done with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000; Thermo Fisher) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000; Invitrogen, Eugene, OR). The sections were mounted onto gelatinized slides and left to dry (at least 2 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (D1306, 1:10,000, Invitrogen) diluted in 1X PBST (1:10,000) ...