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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... (75 mm i.d. 3 2 cm, Acclaim PepMap100 C18 3 mm, 100 A°, ThermoFisher Scientific) and separated over an EASY-Spray column ...
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bioRxiv - Neuroscience 2022Quote: RNA was 3’ labelled with biotin using Pierce™ RNA 3’ End Biotinylation Kit (ThermoFisher) and purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... Head kidney pieces (3 mm × 3 mm) were collected in RNA later (Thermo Fisher Scientific), transferred to 500 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Biophysics 2023Quote: ... 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine (DiI) and 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine (DiD)) were purchased from ThermoFisher Scientific and used without additional purification ...
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed following 3 × 3 min incubations with ACK Lysing Buffer (Gibco). The remaining white blood cells were washed with PBS twice and plated for surface staining ...
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bioRxiv - Biophysics 2022Quote: ... 25mm round glass coverslips were cleaned by incubation with etch solution (5:1:1 ratio of H2O: H2O2 (50% wt in H2O stabilised, Fisher Scientific): NH4OH (ACS reagent ...
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bioRxiv - Microbiology 2019Quote: ... 1 to 5 μl of purified ‘DNA’ and 1 U of the hot-start Taq Platinum polymerase (Invitrogen, Carlsbad, CA, USA). GoTaq (Promega ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed in Pierce IP Lysis Buffer (25 mM Tris HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol) (Thermo Fisher Scientific) containing 1% protease and phosphatase inhibitors on ice and protein concentration was measured by BCA ...
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bioRxiv - Cell Biology 2021Quote: ... 25uM Glutamax and 5% FBS to mouse Neuron culture media (same as mouse Plating media without FBS in ratio of 1:1. (Gibco #17504044)
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bioRxiv - Microbiology 2020Quote: ... samples were washed 5 times with 1× PBS and subsequently incubated with a solution containing 1 µg/mL WGA-txRED (ThermoFisher Scientific), 1 µg/mL 4’ ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Immunology 2019Quote: ... Single suspension of cells were cultured in 48-well plates in the presence of 30 μg mL−1 MOG35–55 for 2h followed by addition of Brefeldin A (5 μg mL−1; Affymetrix/eBioscience) for 4h ...
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bioRxiv - Developmental Biology 2021Quote: Primary antibodies were rabbit anti-eIF4E-5 (1:500; #4524, this work) or rabbit anti-FLAG (1:500; Invitrogen, PA1-984B), and secondary antibodies were Alexa Fluor 488-conjugated anti-rabbit (1:200 ...
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bioRxiv - Immunology 2021Quote: ... The initial inoculum (passage 1) was prepared to dilute the clinical samples (1:5) in non-supplemented DMEM low glucose (Gibco®). The inoculum was then added to the flask containing Vero cells and maintained for 1 hour at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The quality and concentrations of Mf-folD (24.5 ng ·μL-1) and Mf-ftfL (31.3 ng ·μL-1) were determined using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 293T cells were cultured at 37°C in a 5% CO2 humidified incubator in Dulbecco’s modified Eagle’s medium (DMEM)/F12 (1:1) with GlutaMAX™ (Thermo Fisher, UK) media supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Neuroscience 2023Quote: Age synchronized worms were washed thrice with 500 μl M9 buffer in 1.5 ml microfuge tube and incubated in dark for 1 h in 1 ml M9 containing 5 µl DiD dye (Vybrant™ DiD Cell-Labeling Solution, ThermoFisher) at ∼20-30 rpm ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The total dissociated single cells were resuspended in 500 μL sorting buffer (25 mM HEPES, 5 mM EDTA, 1% BSA, in HBSS) containing Normal Rat Serum (1:100; Invitrogen, 10710C) and TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Microbiology 2023Quote: ... pylori pellets were resuspended in PBS and normalized to an OD600 of 1 prior to incubation with 5 μL DNase buffer and 1 unit Turbo DNase I (Life Technologies). After 15 min incubation ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... 1 × TE buffer (5 mM Tris, pH 8.0 and 1 mM EDTA) containing 15% (w/v) PEG-8000 (Fisher Scientific, BP2331), and 510 mM NaCl was added to the origami sample at 1:1 volume in an Eppendorf tube and mixed gently ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 µg plasmids (PBL-CAG-tdTomato-PBR: CMV-PBase = 1:1) were introduced into cells with Lipofectamine 3000 (Thermo Fisher Scientific) using the standard protocol ...
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bioRxiv - Genomics 2023Quote: ... plugs were washed 4 times in 1×Wash Buffer (BNG) and 5 times in 1× TE Buffer (ThermoFisher Scientific, Waltham, MA). Then ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then stained with secondary antibodies diluted 1:500 in 3% BSA and DAPI (Invitrogen D3571) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Constructs were incubated for 1 hour at 37°C with 3 mM Fluo3 AM calcium indicator (ThermoFisher Scientific) in differentiation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Iba1 and caspase-3 were labelled with goat anti-rabbit Alexa Fluor 555 (Invitrogen, A21429; 1:500 dilution), and GFAP and HSP were labelled with goat anti-mouse Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... stained with anti-cleaved caspase 3 secondary antibody (goat anti-rabbit Alexa Fluor 647, Thermo Fisher; 1:400) and anti-Ecadherin secondary antibody (goat anti-mouse Alexa Fluor 555 ...
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bioRxiv - Biophysics 2019Quote: ... 4-(2-(6-(dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)-,hydroxide (di-4-ANEPPS) was purchased from Invitrogen. It was dissolved in ethanol and added to the dried lipid film at a 12:1 lipid:probe molar ratio ...
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bioRxiv - Bioengineering 2020Quote: ... the collagen solution was prepared by mixing 50% (v/v) of 3 mg/mL type 1 collagen (ThermoFisher) with 2.5% of 1N NaOH ...
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bioRxiv - Physiology 2019Quote: ... and 1 µL of either dystrophin (Dmd) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe/primer mix (Applied Biosystems; Mm00464475_m1 and Mm99999915_g1 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were transfected with constructs (co- transfections were performed at 1:3 ratio) using Lipofectamine 2000 (Invitrogen), as described by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... D3 was cultured in DMEM/F12 (3:1) medium supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 8 ng/mL Cholera Toxin (CELL technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... which was performed using a 1:3 ratio of 100 nmol/L siRNAs with Lipofectamine 2000 reagent (Invitrogen) diluted in OptiMEM (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... 10 μg of extracted proteins (n = 3) were solubilized in 1 × NuPage® LDS Sample buffer (Invitrogen, USA) and was run in a 15 well 12% Tris-Gly precast gel (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was stopped by ethanol precipitation: 1 μl 3 M RNAse-free sodium acetate (pH 5.5) (Ambion), 1 μl RNAse-free glycogen (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Braun) were cultured in 3 parts DMEM and 1 part Ham’s F-12 Nutrient Mix (Thermofisher scientific 11765054), supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of DNA was added to 100 μL of optiMEM as well as 3 μL Lipofectamine (Invitrogen) to a separate 100 μL of optiMEM ...
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bioRxiv - Cancer Biology 2021Quote: ... Coverslips were washed 3 times with PBS before the Alexa fluor secondary antibody (1 in 500) (Fisher Scientific) was added and the cells incubated for a further 1 hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged 1:3 when they reached 80% confluence by harvesting cells with 0.25% trypsin EDTA (Gibco).
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bioRxiv - Immunology 2022Quote: ... 1-3 million cisplatin-treated cells were thawed and transferred into Nunc 96 DeepWell polystyrene plates (Thermo Fisher). After two washes with Barcode Perm buffer (Fluidigm) ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron BA.1 (B.1.1.529) were propagated to passage three on Calu-3 cells in Advanced DMEM/F12 (Gibco), supplemented with HEPES ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were incubated for 30 min in standard media with CellEvent Caspase 3/7 (1:400, C10423, Invitrogen) prior to imaging to observe apoptosis.
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bioRxiv - Neuroscience 2020Quote: ... 4) counterstained with the nuclear dye TO-PRO-3 (diluted 1:10’000; Life Technologies, Inc., Gaithersburg, MD, USA). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was diluted 1:3 in distilled water and digested in 0.2 mg/ml proteinase-K (Invitrogen; 25530049) for 10 min at 55 °C ...
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bioRxiv - Cell Biology 2021Quote: ... blocked and stained for F-actin (phalloidin-Alexa Fluor 647; Invitrogen; 1:100 in 3% BSA in PBS) for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: Neocortex from C57Bl/6 pups (postnatal day 1-3) was dissected in Hibernate-A medium (#A1247501, Gibco, USA), digested with papain (#LS003124 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by another 3 washes with DPBS and incubation in 1:500 Alexa Flour 488 (ThermoFisher Scientific, A32723) and 1:10000 DAPI (VWR ...