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Citations for Thermo Fisher :
1751 - 1800 of 2630 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: 8 x 103 HeLa-ACE2 cells and 3.2 x 104 HeLa or HeLa-sgLRRC15 cells (1:4 ratio) were co-plated per well in 8-well chamber slides (Nunc). The following day ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... or 3.8 x 106/ml (Δfut1s) and grown for three days in the presence/absense of 100µM alkyne fucose (Invitrogen C10264), after which parasites were washed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... An S2 cell pellet from a 5 mL culture was resuspended with 400 μL buffer A (50 mM Tris, pH 8, NaCl 150 mM, 20% glycerol and 1x protease inhibitor; Cat# A32965, ThermoFisher). Then the cell lysate was frozen rapidly in liquid nitrogen and thawed in room temperature water bath ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary murine hepatocytes were isolated from livers of WT and mTORLEC mice at 8-12 weeks of age and maintained in DMEM (Gibco) with 10% fetal bovine serum as previously reported (22) ...
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bioRxiv - Biophysics 2021Quote: ... was cultured in Baby Hamster Kidney (BHK)-21 cells at 37°C for 8-10 h with Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, CA, USA) supplemented with 100 U/ml penicillin ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 (2 x 103 cells/well) and MDA-MB-453 (8 x 103 cells/well) were seeded in 96-well plates (Nunc) and allowed to attach overnight ...
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bioRxiv - Cell Biology 2020Quote: ... the GFP-B16 tumor spheroids were transferred to Lab-Tek II cover-glass-bottomed 8-chamber slides and imaged in HBSS supplemented with 2% FBS (all from Invitrogen) using a 63×/1.4 NA oil-immersion objective.
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were loaded for each group in each well and run on 8-16% sodium dodecyl sulfate–polyacrylamide gel (ThermoFisher) for electrophoresis and then transferred to the PVDF membrane ...
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bioRxiv - Cell Biology 2022Quote: ... and 300 μl of resuspended cells in growth medium were transferred to a well of an 8-well chambered coverglass slide (Nunc). Cells were then imaged 48 hours after addition of growth medium using a Zeiss LSM 880 confocal microscope.
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bioRxiv - Immunology 2022Quote: ... 8-16 barcoded samples were pooled and loaded on one Ion 530 chip using the Ion Chef Instrument (ThermoFisher Scientific) and sequenced on the Ion S5 System with 550 flows.
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed on 8-well glass slides at 90% confluent cells using Lipofectamine® 3000 (Cat: L3000075, Invitrogen Co.), following the manufacturer’s instructions and 250 ng of plasmid DNA per well were transfected ...
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bioRxiv - Neuroscience 2022Quote: SOSRS imaged before day 14 were grown on Nunc™ Lab-Tek™ III 8-well chamber slides (Thermo Scientific) and fixed in paraformaldehyde for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: Differentiated cells on day 15 (8 days suspension culture plus 7 days adherent culture) were harvested and dissociated using TrypLE Select (Gibco). Cells were then passed through a 20 µm strainer and resuspended in 1 x PBS with 0.04% BSA buffer ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Neuroscience 2022Quote: Dry protein pellets of both ovary and hemolymph samples were dissolved and reduced in 8 M urea (Fisher Scientific, 45000234)/70 mM ammonium bicarbonate (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... GE Health Sciences) surface was conditioned by injecting 350 mM EDTA (diluted from 0.5 M, pH 8 stock, no. 15575020; Thermo Fisher) at 10 ul/min for 1 minute followed by extensive washing with running buffer to remove residual EDTA ...
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bioRxiv - Developmental Biology 2022Quote: ... the hPSC-PAX3 reporter line was used and the induction efficiency was reported along the 8 day experimental timeline using an inverted microscope (EVOS, Invitrogen). The PAX3 directional bias upon internal force generation was then evaluated in similar manner as the EdU ...
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bioRxiv - Biophysics 2022Quote: ... 2 µg fresh bacmid DNA was transfected into 2 mL Sf9 cells at 0.9×106 cells/2 mL with 8 µL Cellfection II (Thermo Fisher) following manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... 100,000 cells per well were seeded in Nunc™ Lab-Tek™ chambered coverglass 8 well plates (Thermo Fisher Scientific) 24 h prior to transfection ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... alkylated using iodoacetamide in 8M urea denaturation buffer (50mM Tris-HCl, pH 8) and digested overnight in 50mM ammonium bicarbonate using trypsin (ThermoFisher) at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were boiled for 5min at 95°C before being loaded on a 3-8% NuPAGE Tris-Acetate gel (Invitrogen). Western blots were quantified using ImageJ ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep and 8 μg/mL puromycin (to ensure retention of TMPRSS2) with 5% CO2 in a 37°C incubator (ThermoFisher). Cells were trypsinized using 0.05% trypsin and plated to be at 90% confluence the following day ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Electroporated neurons were then transferred to pre-warmed neuronal media and plated at a density of 2,000 cells per well in an 8-well glass Lab-Tek slide (Thermo Scientific) pre-coated with poly-D-lysine (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were heated to 99 °C for 15 min and aliquots (5 µL) resolved by SDS-PAGE on 8 % – 16 % Tris-glycine minigels (Invitrogen) (150 V ...
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bioRxiv - Physiology 2021Quote: CMs isolated from WT and cMKO mice (8-10 weeks) labelled with NucBlue™ Live ReadyProbes™ Reagent (ThermoFisher Scientific) and Tetramethylrhodamine Ethyl Ester Perchlorate (TMRE ...
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bioRxiv - Neuroscience 2020Quote: To quantify oxidative stress/ ROS we used either 8 oxo-dG stainings or CellROX® assays (Thermo Fisher; Green Reagent). 8 oxo-dG (8-oxo-2’-deoxyguanosine ...
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bioRxiv - Microbiology 2021Quote: ... 1×104 transfected cells were seeded into each well of 8-well Lab-Tek™ II chamber slides (ThermoFisher Scientific) for immunostaining analysis ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were plated on poly-L-lysine hydrobromide-coated six-well culture plates for 8 days in Neurobasal Medium (GIBCO) supplemented with B27 ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was extracted from HNs after 8 days of tough decoy and shRNA treatment using TRIzol Reagent (Invitrogen, 15596026) in combination with RNeasy micro kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... carrying the mutation R273C was first amplified by PCR using the primers hp53-1 (5’-CACCATGGAGGAGCCGCAGTCAGATCC-3’) and hp53-8 (5’-GGATCCTCAGTCTGAGTCAGGCCCTTCTGTCTTG-3’) and cloned into the pENTR/D-TOPO vector (ThermoFisher) generating the entry vector pENTR p53(R273C ...
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bioRxiv - Microbiology 2022Quote: Hemolymph sporozoites collected on days 19 to 24 post-infection were centrifuged for 5 min at 450 x g and 4 °C in an 8-well Lab-Tek chamber slide (Nunc) or a 384-well plate (Greiner) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Systems Biology 2022Quote: ... The consistency of plasmid expression throughout different experiments was ensured by transfecting 80 ng of each plasmid per well of an 8-well Lab-Tek™ chambered cover glass slides (0.8 cm2 surface area, ThermoFisher) and the amount of plasmid was scaled up proportional to the culture-well surface area for other culture-plates used ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown for six days at 37 °C with 8% CO2 shaking 125 rpm according to the manufacturer’s protocol (Thermo Fisher). Cell cultures for Spike-W and Spike Omicron BA.1 and BA.2 were harvested six days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... coated Corning dishes at 37°C in a 5% CO2 incubator and gently dissociated for passaging at 1:8 ratio every second day with Stem Pro Accutase (Gibco). Cells were washed with Dulbecco’s Phosphate Buffer Saline cell culture grade.
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bioRxiv - Molecular Biology 2022Quote: ... Cas9 protein was prepared by mixing 12 μl of Alt-R Cas9 protein (62 μM) with 8 μl of buffer R (Invitrogen). gRNA duplexes and Cas9 were then mixed gently and incubated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: Transduced BJ-hTERT cells were seeded in 6-well plates or 8-well chamber slides (Nunc™ Lab-Tek™). Cells were fixed in 4% paraformaldehyde in PBS (Alfa Aesar ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of samples (50-100 µg) were resolved by SDS-polyacrylamide gel electrophoresis in 3–8% Tris-Acetate gels (Thermofisher). Gels were wet-transferred in 1x NuPAGE Transfer Buffer (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... 8% Tris-glycine gels at 150 volts for 2.25 hr or pre-poured NuPAGE 3-8% Tris-Acetate gel (Invitrogen; EA03752) for 2.15 hr (Figure 1C ...
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bioRxiv - Cell Biology 2019Quote: ... dorsal root ganglion neurons were trimmed and isolated from postnatal 8-10 day SD rat spinal cords in HBSS (Invitrogen), and digested in 2.5 U/ml dispase II (Roche ...
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bioRxiv - Molecular Biology 2019Quote: Samples were loaded onto 8% SDS-polyacrylamide gels or 4–12% Tris/HEPES-SDS Bolt™ precast gels (Thermo-Invitrogen) for electrophoresis ...
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bioRxiv - Biophysics 2019Quote: QDs were functionalized with benzyl guanine by mixing 5 μl of 8 μM amino (PEG) QDs emitting at 655 nm (ThermoFisher) with 2 μl of 20 mM BG-GLA-NHS (NEB ...
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bioRxiv - Cell Biology 2019Quote: Cells expressing G12V Rac1A under a tetracycline-inducible promoter along with GFP-WASP or RFP-tagged active Rac marker were resuspended in LoFlo and seeded to a final concentration of 5×105 cells/ml on a borosilicate glass 8 well chamber (ThermoFisher) or on a 35 mm glass bottom dish (MatTek) ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...