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Citations for Thermo Fisher :
1551 - 1600 of 2630 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... we seeded cells onto Geltrex-coated Nunc Lab-Tek 8-well chambered coverglass (Thermo Scientific, cat. 12-565-470) at a density of 4-5×104 cells per well ...
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bioRxiv - Immunology 2021Quote: ... and proteins with a molecular weight above 100 kDa were separated on 3-8% Tris/Acetate gels (Life Technologies) in MOPS Buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Digestion products were resolved in 3-8% tris-acetate SDS-PAGE followed and visualized by Zinc staining (Life Technologies). Images acquisition (Chemi-Doc Camera ...
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bioRxiv - Neuroscience 2020Quote: ... primary DP cells from P5-8 Tgfbr2fl/fl molars were grown in alpha-minimum essential medium (alpha-MEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Systems Biology 2021Quote: ... 75,000 cells were seeded into collagen-1 (Cultrex #3442-050-01) coated 8-well plates (Thermo Fisher Scientific 267062) in GM ...
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bioRxiv - Bioengineering 2021Quote: A standard endodermal differentiation protocol calls for HUES-8 spheroids to be cultured in MCDB131 medium (Thermo Fisher Scientific) containing 200 ng/mL Nodal or 100 ng/mL Activin A (both obtained from R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 3-8% Tris-Acetate gels (Invitrogen, Carlsbad, CA), as described previously [27] ...
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bioRxiv - Biochemistry 2020Quote: ... and 8 pmol of purified DNA was then used for in vitro transcription with T7 TranscriptAid kits (Thermo Scientific). The resulting RNA was purified with Agencourt RNAClean XP beads supplemented with an additional 12% of PEG-8000 and quantified via NanoDrop ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were washed extensively (8 times) in extraction buffer before being resuspended in 1 ml of Trizol (Invitrogen, 15596026) and vortexed vigorously ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Bioengineering 2021Quote: The tissue was washed with 40 μl 1X RT buffer containing 8 μl Maxima 5x RT Buffer (EP0751, Thermofisher), 1 μl RNase Inhibitor (30281 ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells, ATCC CCL-244) were maintained in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: Cells seeded into 8-chamber culture slides (Nunc™ Lab-Tek™ II Chamber Slide™ System, ThermoFisher Scientific) (0.4×106 cells/well ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 μg of whole cell lysate were resolved on a 3-8% Tris-Acetate NuPAGE (Invitrogen) and analyzed by immunoblotting with anti-SPTBN1 ...
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bioRxiv - Immunology 2020Quote: ... following SDS-PAGE in reducing and non-reducing conditions using NuPAGE™ 3-8% Tris-Acetate gels (Life Technologies). Purified proteins were dialyzed overnight against PBS using Slide-A-Lyzer® dialysis cassettes (10 kDa MW cut-off ...
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bioRxiv - Microbiology 2020Quote: ... BS-C-1 cells were seeded into Nunc™ 8-well Lab-Tek II chambered coverglass slides (Thermo Scientific) to obtain 50 to 70% confluence on the day of tracking ...
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bioRxiv - Cell Biology 2021Quote: WA09 (H9) hESC line was purchased from WiCell (wicell.org) and maintained in Essential 8 medium (A151700, Thermo Fisher Scientific) on hESC-qualified growth factor reduced Geltrex-coated (A1413302 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged (0.05% Trypsin EDTA, #25300–054, Gibco, Waltham, MA and 5mg/mL soybean Trypsin inhibitor, #9035-81-8, Gibco) and seeded at 4,000 cells/cm2 into 1× 60 mm and 1× 35 mm collagen-coated culture dishes ...
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bioRxiv - Microbiology 2022Quote: ... pH 8) at room temperature in PierceTM Slide A Lyzer® Mini Dialysis Units (Thermo Fisher Scientific, Waltham, MA). Turbidity measurements at A340 nm were collected in order to quantify the reaction progress by using NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... at 37°C and 8% CO2 to a density of 3.5×106 cells/mL in FreeStyle suspension media (Gibco) supplemented with 2% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of the supernatant was used for SDS-PAGE on 8-12% Tris-Glycine gels (NOVEX™, Invitrogen) with Tris-Glycine SDS Running buffer (NOVEX™ ...
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bioRxiv - Cell Biology 2022Quote: ... livers were perfused in situ with Hank’s balanced salt solution without calcium containing 8 mM HEPES (Thermo Fisher Scientific) via the inferior vena cava after cutting the portal vein to allow outflow of the perfusate ...
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bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: DRG cultures were prepared as described with the neurons plated on 8-well Chamber Slides (154534; Nunc Lab-Tek) coated with poly-D-lysine (P0899 ...
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bioRxiv - Neuroscience 2021Quote: ... HeLa digest was fractionated into 8 fractions using a high pH reversed-phase peptide fractionation Kit (ThermoFisher, Cat. #84868). Twenty μg of HeLa digest was loaded to a C18 solid phase spin column and eluted with a serial concentration of ACN (5% to 50%) ...
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bioRxiv - Plant Biology 2021Quote: ... and PpEF1α were cloned into pCR™8/GW/TOPO™ or pCR™-Blunt (Thermo Fisher Scientific, Inc.). To analyze virulence and protein accumulation in N ...
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bioRxiv - Neuroscience 2020Quote: ... The individual colonies were picked to 24-well matrigel-coated plate containing Essential 8 Medium (E8, Thermo Fisher Scientific) between days 17-28 and passaged with 0.5 mM EDTA weekly ...
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bioRxiv - Neuroscience 2020Quote: ... of brain grey matter tissue was homogenized in 500 μL of 8 M urea lysis buffer (8 M urea, 100 mM Na2HPO4, pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher) using a Bullet Blender (NextAdvance) ...
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bioRxiv - Neuroscience 2021Quote: A solution of 2 μM of Calcein-AM and 8 μM of ethidium homodimer-1 (both from L3224, ThermoFisher) in DPBS (14190-144 ...
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bioRxiv - Microbiology 2022Quote: Between 7×104 and 1.2×105 HepG2 cells (ATCC) per well were seeded in an 8-well Lab-Tek chamber slide (Nunc), precoated with rat tail collagen type I (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
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bioRxiv - Genetics 2022Quote: ... Cells were pelleted at 3,500 x g for 8 minutes and the pellet resuspended in 1 ml of Trizol (Invitrogen) and incubated for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
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bioRxiv - Microbiology 2022Quote: ... interferon-β treated (250 U/ml for 8 h) or infected with influenza WSN (MOI 0.02 for 24 h) using TRIzol (Invitrogen). Biologic triplicate sample RNA was sent for library preparation and paired-end RNA-seq by Novogene (BioProject PRJNA667475) ...
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bioRxiv - Neuroscience 2019Quote: Single-molecule FRET measurements were carried out using ~30 pM of labelled tau in phosphate buffer (40 mM potassium phosphate, 50 mM KCl, pH 7.4) in 8-chambered Nunc coverslips (ThermoFisher) passivated with poly(ethylene glycol ...
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bioRxiv - Immunology 2019Quote: ... while proteins above 200 kDa were blotted on a 3-8% Tris-Acetate gels in MOPS Buffer (Life Technologies). Proteins were separated at 200V for approximately one hour and transferred onto either PVDF or nitrocellulose membrane using the TransBlot Turbo Transfer (BioRad ...
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bioRxiv - Genetics 2019Quote: ... undifferentiated cells were grown in a feeder-free culture conditions using defined Essential 8 medium combined with vitronectin-(Gibco)-coated surfaces ...
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bioRxiv - Microbiology 2019Quote: ... 115 rpm and 8% CO2 following the Max Titer protocol with an additional feed on day five (Thermo Fisher). Culture supernatants were harvested 13 to 14 days after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... 1 × 104 293T-GRET/-PalmGRET cells were seeded per well into a glass-bottom 8-well chamber (#1.5, Nunc™ Lab-Tek™ II Chamber Slide™ System ...
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bioRxiv - Cell Biology 2020Quote: ... Vector particles were produced from second day supernatants conditioned by 293FT cells transduced using 8 μg/ml polybrene (Invitrogen) (Szulc and Aebischer ...
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bioRxiv - Neuroscience 2021Quote: ... Indels were introduced by transfecting 8×105 cells with 1.5 μg plasmid in 100 μl scale using the Neon Transfection System (ThermoFisher) with one pulse at 1500 millivolts for 30 milliseconds (∼40-70% transfection efficiency) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human oral cancer cell line OC3-IV2 cells have been described [8–10] and were cultured in a 1:1 ratio of Dulbecco’s modified Eagle medium (DMEM; Invitrogen) and keratinocyte serum-free medium (KSFM ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were polymerized for 2 h at 37°C and then digested with 8 U/ml Proteinase K (Invitrogen) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 min) and resuspended in 1 ml PBS containing 8 µl UltraPure BSA (50mg ml−1; AM2616, ThermoFisher Scientific) and filtered over Flowmi 40µm cell strainers (VWR ...
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bioRxiv - Molecular Biology 2021Quote: ... On the 15th day of reprogramming N2B27 medium was switched to complete Essential 8 medium (E8; Thermo Fisher Scientific), which was then changed every two days ...
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bioRxiv - Molecular Biology 2021Quote: The droplets were re-injected in the first pico-injector device with the shorter re-injection channel (Figure S1C) and coalescence-induced merging with a poly(A) solution consisting of 26.6 mM Tris-HCl pH 8 (Invitrogen), 15.8 mM DTT (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products (~240-290 bp) were purified by gel extraction on an 8% TBE polyacrylamide gel (Thermo Fisher Scientific) and isopropanol precipitation ...
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bioRxiv - Biophysics 2021Quote: ... P2 viruses (8%) were used to infect tsA201 cells grown in Freestyle 293 Expression Medium in suspension culture (ThermoFisher). Infected cells were incubated for an initial 12 h at 37 °C before 10 mM sodium butyrate was added ...
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bioRxiv - Immunology 2020Quote: ... fraction was separated by centrifugation at 10,000 x g for 30 minutes at 2-8 °C (Sorvall Lynx 6000, ThermoFisher). The IB pellet was washed with 20 mM HEPES (pH 8.0) ...