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Citations for Thermo Fisher :
1651 - 1700 of 2630 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Epithelial layer was removed by incubation (20 min, 37°C) in 10 ml HBSS (Ca-Mg-) containing EDTA (0.5M, pH 8, Invitrogen) and HEPES (1M ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of sample solution was loaded in chambered coverglasses with 8 wells (Nunc LabTek II or ibidi μSlides), which had been pre-coated with poly-L-lysine to facilitate cell attachment ...
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bioRxiv - Developmental Biology 2023Quote: ... and p-Smad1/5/8 and thoroughly analyzed under a fluorescent light microscope (EVOS FL, Life Technologies, Carlsbad, CA). Single-plane laser scanning confocal photomicrographs were taken from every other section using a laser scanning confocal microscope (Zeiss LSM 710 ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from larvae in groups of 8 using TRIzol according to manufacturer instructions (Invitrogen; 15596–018). RNA samples were then DNase-treated with Ambion Turbo DNase according to manufacturer’s instructions (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: HCT116 and UMUC3 cells were transferred to 8 well glass chamber slides and maintained in Dulbecco’s Modified Eagle Medium (Invitrogen) with 10% tet-free fetal bovine serum ...
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bioRxiv - Plant Biology 2023Quote: ... the coding sequence of each protein was PCR-amplified and subcloned into pCR™8/GW/TOPO® (Invitrogen). To generate binary plasmids ...
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bioRxiv - Cancer Biology 2023Quote: TR14 cells for interphase FISH were grown in 8-chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™) to 80 % confluence ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 95C and subjected to NuPage 3-8% Tris-Acetate gel (#EA0375PK2, Invitrogen). Separated proteins were transferred to cellulose membrane using a semi-dry transfer method and blocked in TBS-T with 5% dry milk containing 2% normal goat serum for 1hr ...
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bioRxiv - Microbiology 2023Quote: HCT-8 cells were plated at 4.5 x 105 cells per 12-mm-diameter glass coverslip (Thermo Fisher Scientific) in 24-well tissue culture plates and incubated until confluency (∼24 h) ...
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bioRxiv - Biophysics 2023Quote: ... were grown at 37 °C and 8% CO2 to a density of 3.5 × 106 cells/mL in FreeStyle suspension media (Gibco) supplemented with 2% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... the culture medium in the top channel inlet reservoir was replaced with Essential 8 medium (Cat. #: A1517001, ThermoFisher, USA), and perfusion commenced ...
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bioRxiv - Cell Biology 2023Quote: ... S12+ media was changed at day 8 and replaced at day 10 with RPMI (ThermoFisher Scientific, Waltham, MA, USA) media supplemented with 213 µg/µL L-ascorbic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... were plated in 8 µL of 10µM OA-supplemented phenol-red– free Roswell Park Memorial Institute (RPMI) complete growth media (Gibco) into black-walled clear-bottom 1536-well plates ...
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bioRxiv - Bioengineering 2023Quote: ... The solution was transferred to dialysis membranes (12 -14 kDa molecular weight cut off; Fisher Scientific 21-152-8) and dialyzed against deionized water for 7 days and subsequently lyophilized (LABCONCO).
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bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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bioRxiv - Cell Biology 2023Quote: ... or 30.000 cells (exo-erythrocytic development) were seeded per well of an 8-well Permanox Lab-Tek chamber slide (Nunc). After two hours of incubation with sporozoites at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Each sample (8 μl) was analyzed by LC-MS/MS on an LTQ Orbitrap XL (Thermo Scientific, Waltham, MA) coupled to the nanoACQUITY UPLC system (Waters) ...
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bioRxiv - Plant Biology 2023Quote: ... the coding sequence of MYB36 [8] was Gateway-cloned into a pDONR221 entry vector using BP clonase II (Invitrogen) according to manufacturer’s description ...
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bioRxiv - Molecular Biology 2023Quote: ... were isolated from adult mice aged 8 weeks and was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% fetal bovine serum (FBS, GIBCO, Brazil) in a humidified atmosphere at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... The RNase T1 ladder was prepared by denaturing 0.4 pmol of 5′ labelled Aar with 8 µL of 1× sequencing buffer (Ambion) at 95°C for 1 min ...
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bioRxiv - Molecular Biology 2023Quote: 8–10 ng of plasmid pools (pHPSC1142, pHPSC1160, pHPSC1117) were used in PCR using Phusion polymerase (Thermo Fisher F530S) or Phusion Flash High-Fidelity PCR Master Mix (Thermo Fisher F548L) ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel attached were transferred to a well of a 6-well plate with 1 ml of digestion solution (0.5% Triton X100, 1 mM EDTA pH 8 (AM9260G, Ambion), 50 mM Tris pH 8 (AM9855G ...
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bioRxiv - Cell Biology 2023Quote: ... 8 µg of protein was separated on a NuPAGE 4–12% Bis-Tris gel (Life technologies, Carlsbad, CA, USA). Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P ...
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bioRxiv - Bioengineering 2023Quote: ... Whole conditioned media from flasks and bioreactors was 0.2 μm filtered prior to first round UC at an RCFmax of 133,900 x g (Sorvall WX ultraCentrifuge, ThermoFisher; Fiberlite F37L-8×100 Fixed-Angle Rotor, ThermoFisher, k factor=168 ...
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cells were imaged in 8-well Nunc™ Lab-Tek™ II chambered coverglass (Cat #154409; Thermo Scientific). All images were taken as single slices at approximately mid-plane using a Zeiss LSM880 inverted laser scanning confocal microscope equipped with Zen software ...
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bioRxiv - Biochemistry 2023Quote: ... Handcast SDS-PAGE gels (8%, 10%, 15%, or 4-18% gradient) containing 0.5% (v/v) trichloroethanol (Acros Organics 139441000) for stain-free imaging (79 ...
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bioRxiv - Physiology 2024Quote: ... maintained at 8°C and coupled to an Orbitrap ELITE™ Hybrid Ion Trap-Orbitrap Mass Spectrometer (ThermoFisher Scientific). 5 µL of extract were injected into an Agilent Poroshell 120 EC-C18 column (2.1 x 100 mm ...
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bioRxiv - Plant Biology 2024Quote: ... in each condition total RNA was extracted from a pool of 8 leaves and extracted using TRIzol reagent (Invitrogen). Three independent biological replicates were analysed ...
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bioRxiv - Bioengineering 2024Quote: ... and treated with purified viral particles resuspended in 0.8 mL DMEM containing 8 µg/mL polybrene (Thermo Fisher Scientific). The cells were spinfected by centrifuging the six-well plates at 1050 x g for 90 min at 30 °C and the returned to the 37 °C incubator for another 48 h ...
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bioRxiv - Plant Biology 2024Quote: ... 8 and 6 hours were then transferred to MS medium containing zeocin with Actinomycin D (Thermo Fisher, J60148.LB0) or zeocin with DMSO (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Colons and small intestines (without caecum) from female mice (8-20wks) were isolated and transferred into RPMI-1640 (Gibco) medium supplemented with L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 8-10 times in PBS for 5 minutes and incubated with DAPI (0.1 mg/ml in PBS; #62248, Invitrogen) to stain nuclei ...
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bioRxiv - Microbiology 2024Quote: ... HCT-8 cells were cultured in maintenance medium RPMI 1640 with GlutaMAX™ and HEPES supplements (ThermoFisher Scientific # 72400047) plus 5% foetal calf serum (FCS ...
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bioRxiv - Zoology 2024Quote: ... injected embryos were frequently cultured through stage 8 in HL saline containing roughly 1% commercial penicillin-streptomycin solution (Gibco).
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bioRxiv - Biochemistry 2023Quote: ... SDS-PAGE was carried out on either NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in Table S5.
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bioRxiv - Developmental Biology 2024Quote: ... Medium was replaced daily from 8 hpf and supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) to inhibit melanogenesis ...
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bioRxiv - Molecular Biology 2024Quote: Circular mtDNA was first linearized by adding 12.5 U/μL MssI enzyme (Pmel, 8-bp endonuclease) (ThermoFisher, MA, USA) for 2 h at 37 °C in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected into HepaCam enriched astrocytes seeded in 8-well chamber slides using the lipofectamine LTX reagent (0.6μl) and plus reagent (0.2μl, Thermo Fisher Scientific, A1262) containing 40μl Opti-MEM following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Frozen samples (n=8) were OCT embedded and sectioned at 10μm on a Cryostar NX70 cryostat (Thermo Fisher Scientific). Capture sections were fixed with methanol ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted at 800g for 10 min at 4°C and washed twice in buffer 1 and once in freezing buffer (1M Tris pH 8, 100% glycerol, 100mM MgAc2, 0.5M EDTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion). Nuclei were resuspended in freezing buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL reactions were prepared with 8 nM SWR1C mixed with 0.5 μM phosphate sensor (fluorophore-conjugated phosphate binding protein, ThermoFisher) in remodeling buffer ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel stuck to it were transferred to a 6-well plate with 1 ml of digestion solution (0.5 % Triton X100, 1 mM EDTA, pH 8 (AM9260G, Ambion), 50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: For the co-immunoprecipitation (CoIP) 8 μl affinity-purified anti-CP31A antibody (Kupsch et al., 2012) was bound to 50 μl Dynabeads ProteinG (Invitrogen) under rotation (15 rpm) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
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bioRxiv - Cell Biology 2020Quote: ... the strips were washed 3 times in PBS-T before incubation in primary antibody (GST Tag Mouse anti-Tag, Clone: 8-326, Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 3.2 μg of p0G44 (pSB2380) and 1 μg of pSB2860/pSB2863 were mixed with 8 μL of Lipofectamine2000 (Invitrogen 11668027) in 400 μL of DMEM (no supplements ...
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bioRxiv - Cell Biology 2020Quote: ... IP and lysate samples were separated by SDS-PAGE on precast 3-8% tris-acetate and 4-12% bis-tris acrylamide gels (Invitrogen) followed by transfer to 0.2 μm nitrocellulose membranes (Amersham) ...