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Citations for Thermo Fisher :
1851 - 1900 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). For σ28 purification ...
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bioRxiv - Genetics 2019Quote: A set of 4x 40 µl of PCR reaction was mixed with 8 µl 6x DNA load dye (Thermo Scientific) and run on a 2% TBE agarose gel (UltraPure ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Cancer Biology 2020Quote: ... ATM and ATR in the CE samples as follows: the samples were loaded on 4-8% (w/v) Tris-acetate SDS-PAGE gels (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... were lysed in urea buffer (8 M urea, 100 mM NaHPO4, pH 8.5) with HALT protease and phosphatase inhibitor (Pierce/Thermo Scientific) and processed at the Emory Proteomics Core ...
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bioRxiv - Microbiology 2021Quote: ... A 1ml-overlay of 1:1 mixture of 2X Minimal Essential Medium (MEM; Hyclone, SH30008.04) with 8% FBS and 1.5% low melting agarose (ThermoFisher Scientific, R0801) was placed in the wells ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 mg Orange G in 10 mL H2O) was added to each reaction and run on an 8% TBE gel (Invitrogen) (180 V ...
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bioRxiv - Neuroscience 2020Quote: ... siRNAs and Gapmers or shRNAs were introduced to primary hippocampal neurons (5–8 days in vitro (DIV)) using Lipofectamine RNAiMAX or Lipofectamine 2000 (Invitrogen) according to manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Purified proteins were dialyzed in Spectra-Por7 semi-permeable dialysis membranes (8 kDa cut-off; Thermo Fisher Scientific, Waltham, MA) for 48 hours against conservation buffer (20 mM Tris pH=8.0 ...
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bioRxiv - Biochemistry 2020Quote: Cells were trypsinized and seeded at 20,000 cells/well in an 8-well chambered coverglass (Nunc™, 12-565-470) with fresh DMEM (+ ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were then transferred to pre-warmed neuronal media and plated at a density of 2,000 cells per well in an 8-well glass Lab-Tek slide (Thermo Scientific) pre-coated with poly-D-lysine (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... seeded at 1.5 x 105 neurons per coverslip in Neuron Medium (8 % FBS, 2 % B-27, [Gibco, 17504-044], Penicillin [200 U/mL]/Streptomycin [200 μg/mL] [Gibco, 15140-130] ...
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bioRxiv - Neuroscience 2020Quote: A craniotomy was performed on 8-week-old mice and 200nl of a 10 μg/ml solution of Hoechst 33342 (ThermoFisher) was injected in multiple spots in 200 – 300 μm depth ...
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bioRxiv - Molecular Biology 2019Quote: Alternative oxidase (AOX) cDNA from Pacific oyster (Crassostrea gigas) (Genbank, #ACL31211) (8) was cloned into the pcDNA6A mammalian expression vector (Invitrogen). First ...
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bioRxiv - Cell Biology 2021Quote: Extracts were analyzed by SDS–PAGE using a 4-12% Bis–Tris or 3-8% Tris-Acetate NuPAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... Mφ were plated in an 8-well chamber slide (Nunc Lab-Tek II Chamber Slide System, ThermoFisher scientific, Massachusetts, USA) (50,000 cells/well) ...
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bioRxiv - Genomics 2020Quote: ... The DNA was eluted in 150 μL 10 mM Tris-HCl pH 8 and the biotinylated molecules captured with Dynabeads M280 (Invitrogen) according to the manufacturer’s protocol but using150 μL Bead Binding buffer (Phase Genomics ...
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bioRxiv - Bioengineering 2021Quote: ... was performed by incubating the tissue-attached HDMI-array in 40 μl RT reaction solution containing 8 μl Maxima 5x RT Buffer (EP0751, Thermofisher), 8 μl 20% Ficoll PM-400 (F4375-10G ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured on 8-well Nunc™ Lab-Tek™ II chambered coverglass (155409; Thermo Scientific™, Waltham, MA). For gene expression studies ...
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bioRxiv - Cell Biology 2021Quote: ... dried peptide sample was resuspended in 200 mM HEPES (pH 8) and incubated for 1 h at room temperature with one of the TMT10-plex reagents (ThermoFisher) solubilized in 100% anhydrous ACN ...
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bioRxiv - Plant Biology 2020Quote: ... five-day-old seedlings were transferred to new MS plates supplemented with or without 8 μM zeocin (Invitrogen Life Technologies). For aluminium treatments ...
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bioRxiv - Plant Biology 2020Quote: ... five-day-old seedlings were transferred to new MS plates supplemented with or without 8 μM zeocin (Invitrogen Life Technologies). For aluminium treatments ...
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bioRxiv - Systems Biology 2020Quote: ... We then added 8 μL/well of RT mix to the plate: 4 μL of 5x RT Buffer (Thermo Scientific), 0.35 μL of SUPERase-In RNAse Inhibitor (20 U/μL ...
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bioRxiv - Microbiology 2021Quote: ... Transfection was performed on 8-well glass slides at 90% confluent cells using Lipofectamine® 3000 (Cat: L3000075, Invitrogen Co.), following the manufacturer’s instructions and a total of 250 ng of DNA per well was transfected ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Microbiology 2021Quote: 8 x 103 HeLa-ACE2 cells and 3.2 x 104 HeLa or HeLa-sgLRRC15 cells (1:4 ratio) were co-plated per well in 8-well chamber slides (Nunc). The following day ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... or 3.8 x 106/ml (Δfut1s) and grown for three days in the presence/absense of 100µM alkyne fucose (Invitrogen C10264), after which parasites were washed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... An S2 cell pellet from a 5 mL culture was resuspended with 400 μL buffer A (50 mM Tris, pH 8, NaCl 150 mM, 20% glycerol and 1x protease inhibitor; Cat# A32965, ThermoFisher). Then the cell lysate was frozen rapidly in liquid nitrogen and thawed in room temperature water bath ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary murine hepatocytes were isolated from livers of WT and mTORLEC mice at 8-12 weeks of age and maintained in DMEM (Gibco) with 10% fetal bovine serum as previously reported (22) ...
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bioRxiv - Biophysics 2021Quote: ... was cultured in Baby Hamster Kidney (BHK)-21 cells at 37°C for 8-10 h with Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, CA, USA) supplemented with 100 U/ml penicillin ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 (2 x 103 cells/well) and MDA-MB-453 (8 x 103 cells/well) were seeded in 96-well plates (Nunc) and allowed to attach overnight ...
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bioRxiv - Cell Biology 2020Quote: ... the GFP-B16 tumor spheroids were transferred to Lab-Tek II cover-glass-bottomed 8-chamber slides and imaged in HBSS supplemented with 2% FBS (all from Invitrogen) using a 63×/1.4 NA oil-immersion objective.
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were loaded for each group in each well and run on 8-16% sodium dodecyl sulfate–polyacrylamide gel (ThermoFisher) for electrophoresis and then transferred to the PVDF membrane ...
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bioRxiv - Cell Biology 2022Quote: ... and 300 μl of resuspended cells in growth medium were transferred to a well of an 8-well chambered coverglass slide (Nunc). Cells were then imaged 48 hours after addition of growth medium using a Zeiss LSM 880 confocal microscope.
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bioRxiv - Immunology 2022Quote: ... 8-16 barcoded samples were pooled and loaded on one Ion 530 chip using the Ion Chef Instrument (ThermoFisher Scientific) and sequenced on the Ion S5 System with 550 flows.
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed on 8-well glass slides at 90% confluent cells using Lipofectamine® 3000 (Cat: L3000075, Invitrogen Co.), following the manufacturer’s instructions and 250 ng of plasmid DNA per well were transfected ...
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bioRxiv - Neuroscience 2022Quote: SOSRS imaged before day 14 were grown on Nunc™ Lab-Tek™ III 8-well chamber slides (Thermo Scientific) and fixed in paraformaldehyde for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: Differentiated cells on day 15 (8 days suspension culture plus 7 days adherent culture) were harvested and dissociated using TrypLE Select (Gibco). Cells were then passed through a 20 µm strainer and resuspended in 1 x PBS with 0.04% BSA buffer ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Neuroscience 2022Quote: Dry protein pellets of both ovary and hemolymph samples were dissolved and reduced in 8 M urea (Fisher Scientific, 45000234)/70 mM ammonium bicarbonate (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... GE Health Sciences) surface was conditioned by injecting 350 mM EDTA (diluted from 0.5 M, pH 8 stock, no. 15575020; Thermo Fisher) at 10 ul/min for 1 minute followed by extensive washing with running buffer to remove residual EDTA ...
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bioRxiv - Developmental Biology 2022Quote: ... the hPSC-PAX3 reporter line was used and the induction efficiency was reported along the 8 day experimental timeline using an inverted microscope (EVOS, Invitrogen). The PAX3 directional bias upon internal force generation was then evaluated in similar manner as the EdU ...
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bioRxiv - Biophysics 2022Quote: ... 2 µg fresh bacmid DNA was transfected into 2 mL Sf9 cells at 0.9×106 cells/2 mL with 8 µL Cellfection II (Thermo Fisher) following manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... 100,000 cells per well were seeded in Nunc™ Lab-Tek™ chambered coverglass 8 well plates (Thermo Fisher Scientific) 24 h prior to transfection ...