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Citations for Thermo Fisher :
1601 - 1650 of 2630 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: Induced pluripotent stem cells were maintained in E8™ medium with Essential 8™ supplement (Gibco-Thermo Fisher Scientific) and passaged once every 5-6 days using Dispase (StemCell Technology) ...
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bioRxiv - Neuroscience 2019Quote: Induced pluripotent stem cells were maintained in E8™ medium with Essential 8™ supplement (Gibco-Thermo Fisher Scientific) and passaged once every 5-6 days using Dispase (StemCell Technology) ...
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bioRxiv - Genetics 2020Quote: ... The PCR was performed in MicroAmp Fast Optical 96-Well Reaction Plates or Optical 8-Well Strips (Applied Biosystems) by first heating to 95°C for 5 minutes before running through 40 cycles of 95°C for 15 seconds ...
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bioRxiv - Genetics 2020Quote: ... Samples (5 μL each) were loaded into wells of a NuPAGE Tris-acetate 3 – 8% polyacrylamide gel (ThermoFisher Scientific) and electrophoresed for 60 min at 15 volts/cm ...
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bioRxiv - Cell Biology 2020Quote: ... Clinical hESCs were maintained in commercially available Essential 8™ (E8) basal medium (Gibco, Carlsbad, MO, USA; A15169-01) with Essential 8™ Supplement (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: BMDMs were seeded at 1×105 cells/well in 8 wells Nunc Lab-Tek II Chamber Slide (Thermo Scientific). The next day ...
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Biochemistry 2021Quote: ... and for nucleic acids using a 12.5 % polyacrylamide/8 M urea gel visualized by SYBR Gold staining (ThermoFisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fibroblast chemotaxis was measured using 24-well Nunc (8-µm pore size) Transwell inserts (Thermo Fisher, Waltham, MA, USA). Following confluence ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysosomal ExRai AMPKAR was made by inserting PCR-amplified LAMP149 generated using primers 7-8 into HindIII (ThermoFisher FD0504)/BamHI-digested ExRai AMPKAR backbone ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells; ATCC CCL-244) were cultured in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2019Quote: Huh-7 and C6/36 cells were grown in 8 well chambers (LAB-TEK®, Nalgene Nunc International, USA) for 24h ...
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bioRxiv - Microbiology 2021Quote: Bone marrow derived macrophages (BMDMs) were generated from 6-8 week old BALB/c mice in RPMI-1640 (Gibco) containing 10% foetal calf serum ...
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bioRxiv - Immunology 2020Quote: ... the implants were washed once with 1XPBS and placed in 8-well chamber slides (Thermo Fisher Scientific, Waltham, MA) (Figure 1) ...
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bioRxiv - Immunology 2021Quote: ... 8 µm sections were cut using a cryostat and sections were mounted on SuperFrost Plus glass slides (ThermoFisher Scientific). Sections were incubated in ice-cold acetone for 10 min prior to blocking in 5% BSA in PBS ...
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bioRxiv - Immunology 2020Quote: ... cells were quickly lysed in 8% Lithium dodecyl sulfate LDS loading buffer preheated at 85°C (Invitrogen Cat#NP0007) and incubated with stirring at 500 rpm for 5 min at 85°C (Eppendorf ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were seeded at 70% confluency in 8 well chambers and transfected with lipofectamine 2000 (ThermoFisher Scientific, 11668030). For RAW macrophages ...
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bioRxiv - Microbiology 2021Quote: ... 115 rpm and 8% CO2 following the Max Titer protocol with an additional feed on day five (Thermo Fisher). Culture supernatants were harvested 13 to 14 days after transfection ...
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bioRxiv - Immunology 2022Quote: ... Ampho cells were washed with OptiMEM and transfected with 8 ug of retroviral vector using Lipofectamine 3000 (16 μL) and PLUS reagent (40 μL) following manufacturer’s instructions (Invitrogen) 113 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in Essential 8 (made in-house based on (Chen et al., 2011)) on Vitronectin (Life Technologies), and were maintained at 37°C under a humidified atmosphere of 5% CO2 in air ...
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bioRxiv - Cancer Biology 2022Quote: HCI-EC-23 and Ishikawa cells were grown in regular media in 8-well chamber slides (Thermo Scientific, 177402PK). Cells were washed with 1x PBS and then fixed using 4% formaldehyde (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... and were detached from the 8-well chamber slides using 100 μL Trypsin-EDTA (ThermoFisher Scientific, catalog no. 25200056). Cells were then resuspended using 100 μL of DMEM (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... NCC primary cultures were performed in 4-well plates or in 8-well Chambered Coverglass (Nunc, cat. no. 154511) coated with fibronectin ...
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bioRxiv - Cell Biology 2022Quote: ... Cells used for microscopy were plated in LabTek 8 well dishes and were transiently transfected using Lipofectamine 3000 (ThermoFisher) using 2.5 mg/ml of CD63-pHluorin plasmid DNA (gift from D.M Pegtel27 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and plated onto coverslips (1:2 split) or Nunc 8 well chamber slides (Thermo Fisher # 155409PK) (20-40k cells per well).
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bioRxiv - Immunology 2022Quote: ... using 4.5 mL to transform 8 to 10 million PBMCs in B cell growth medium (Medium A containing CpG [Invitrogen, oligo ZOEZOEZZZZZOEEZOEZZZT] at the 10 μmole scale [desalted] ...
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bioRxiv - Genetics 2022Quote: ... 8 μl of the eluted DNA were transformed into 20 μl of electrocompetent cells (MegaX DH10B, Thermo Fisher Scientific) according to the manufacturer’s protocol using the ECM 399 electroporator (BTX) ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: Fibroblasts were seeded in an 8-well glass-bottomed chamber (#155411; Nunc Lab-Tek Thermo Scientific, Waltham, MA, USA), three days prior to the experiment ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were lysed in 8 M urea lysis buffer (8 M urea, 10mM Tris, 100 mM NaH2PO4, pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher). Homogenization was performed using a Bullet Blender (Next Advance ...
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bioRxiv - Immunology 2022Quote: ... T cell subsets before injection and recovered at day 8 post-HCT were frozen in Trizol (Thermo Fisher Scientific) and conserved at −80°C until analysis.
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bioRxiv - Systems Biology 2022Quote: ... The protein fractions were quantified using an 8 point Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat.# PI23225), and 20 µg of protein per sample diluted to 35µL using NuPAGE LDS sample buffer (1x final conc. ...
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bioRxiv - Plant Biology 2023Quote: ... Yields (8-21 μg at 350-900 ng/μL) were determined using the Qubit fluorometer (ThermoFisher, Waltham, MA, USA). Fragments of >40 Kbp were collected for library preparation using BluePippin pulsed-field electrophoresis (Sage Science ...
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bioRxiv - Molecular Biology 2022Quote: ... For the RNase T1 ladder 0.2 pmol of labeled sRNA was incubated with 8 μL of 1x sequencing buffer (Ambion) at 95 °C for 1 min followed by the addition of 1 μL RNase T1 (0.1 U/ μl ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from samples collected within 8 days of symptoms onset using either KingFisher Flex System (ThermoFisher Scientific) or Extracta 96 (Loccus ...
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bioRxiv - Cancer Biology 2023Quote: Corning costar plates (#3422 and #3428) with polycarbonate membrane and 8 micron pore size were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Blood was collected from hearts of freshly sacrificed mice to tubes coated with 0.5 M EDTA pH 8 (Invitrogen) to prevent clotting ...
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bioRxiv - Immunology 2023Quote: ... and finally glued with picodent twinsil extrahart (Picodent) to the bottom of 8- or 16-well LabTek chambers (Nunc), from which the original glass bottom had been removed ...
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bioRxiv - Cell Biology 2023Quote: ... 25% of the total immunoprecipitated material (per condition) was loaded onto a 3-8% Tris-Acetate gel (Thermo Fisher), fully resolved gels were transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2023Quote: ... and loaded to gels (8 μL/well) for SDS-PAGE and Western blotting (NuPage Novex system, Thermo Fisher Scientific) to 0.45 μm nitrocellulose membranes (BioRad laboratories).
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bioRxiv - Neuroscience 2023Quote: ... at DIV 8 the astrocytes were transfected with the expression plasmids using Lipofectamine LTX with Plus Reagent (Thermo Scientific) per the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... IMCD3 cells were seeded in 8-well chamber slides (Nunc™ Lab Tek™ II Chamber Slide™ System) followed by siRNA treatment the next day with either the control siRNA or Adcy3 ...
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bioRxiv - Microbiology 2023Quote: HCT-8 cells were plated at 4.5 x 105 cells per 12-mm-diameter glass coverslip (Thermo Fisher Scientific) in 24-well tissue culture plates and incubated until confluency (∼24 h) ...
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bioRxiv - Biophysics 2023Quote: ... were grown at 37 °C and 8% CO2 to a density of 3.5 × 106 cells/mL in FreeStyle suspension media (Gibco) supplemented with 2% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 95C and subjected to NuPage 3-8% Tris-Acetate gel (#EA0375PK2, Invitrogen). Separated proteins were transferred to cellulose membrane using a semi-dry transfer method and blocked in TBS-T with 5% dry milk containing 2% normal goat serum for 1hr ...
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bioRxiv - Cell Biology 2023Quote: ... boiled at 95°C for 5 min and loaded into a NuPAGE 3-8% Tris-Acetate Gel (Thermo Scientific) along with HiMark™ Pre-stained Protein Standard (Thermo Scientific LC5699) ...
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bioRxiv - Immunology 2023Quote: ... Biopsies were first transferred in the dissociation medium consisting of 10 ml HBSS (free of calcium and magnesium) containing EDTA (0.5M, pH 8, Invitrogen) and HEPES (1M ...
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bioRxiv - Genomics 2023Quote: ... A second reaction mixture (8 μL/reaction) containing the reaction buffer (5µL of First strand Buffer 5X, Invitrogen, France), DTT (Dithiothreitol ...
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bioRxiv - Cancer Biology 2023Quote: TR14 cells for interphase FISH were grown in 8-chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™) to 80 % confluence ...
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bioRxiv - Immunology 2023Quote: ... for 8 minutes at room temperature and then stopped using 3M hydrochloric acid (Fisher Scientific, Cat No. A144-212). Plates were read at 490nm using a BioTek Synergy2 plate reader and Gen5 (v3.09 ...
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bioRxiv - Cancer Biology 2023Quote: TR14 cells for interphase FISH were grown in 8-chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™) to 80 % confluence ...